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Triwidodo Arwiyanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary

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MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.855 KB) | DOI: 10.23960/j.hptt.11564-71

Abstract

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.
POTENSI TIGA GENUS BAKTERI DARI TIGA RIZOSFER TANAMAN SEBAGAI AGENSIA PENGENDALI HAYATI PENYAKIT LINCAT Heru Adi Djatmiko; Triwidodo Arwiyanto; Bambang Hadisutrisno; Bambang Hendro Sunarminto
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 9, No 1 (2007)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.9.1.40-47

Abstract

Objectives of this research were to characterize three genera of bacteria isolated from three of crop rhizosphere, and to measure the ability of the antagonistic bacteria in suppressing lincat disease caused by Ralstonia solanacearum and Meloidogyne incognita. The research showed that the sixth bacteria were able to utilize some carbon and nitrogen compounds, degrade macromolecules, grew at different temperatures and salt contents, and grew at medium with chitin and pectin.  The bacteria isolated from pepper rhizosphere (Pf51, Ba4, Ba22), groundnut (Pf83), and eggplant (S4 dan S7) was included to fluorescent pseudomonads (Pf51 and Pf 83), Bacillus spp. (Ba4 and Ba22), and Streptomyces spp. (S4 and S7).  The sixth bacteria having the ability in antagonist.  The bacteria isolate having the best ability in suppressing Ralstonia solanacearum and Meloidogyne incognita was Streptomyces spp. (S4). The bacteria isolate having the best ability in suppresssing R. solanacearum by antibiosis and the inhibition mechanism by bacteriostatic was S4
PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN Bacillus spp. DAN Pseudomonad fluoresen CHRISNAWATI CHRISNAWATI; NASRUN NASRUN; TRIWIDODO ARWIYANTO
Jurnal Penelitian Tanaman Industri Vol 15, No 3 (2009): September 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n3.2009.116-123

Abstract

ABSTRAKPenelitian pengendalian penyakit layu bakteri nilam (Ralstoniasolanacearum) menggunakan Bacillus spp. dan Pseudomonad fluoresen dikebun petani nilam di Nagari Kajai, Pasaman Barat, Sumatera Barat telahdilakukan pada bulan Mei sampai November 2006. Penelitian ini bertujuanuntuk mendapatkan Bacillus spp. dan Pseudomonad fluoresen yangberpotensi untuk mengendalikan penyakit layu bakteri, dan meningkatkanpertumbuhan dan produksi nilam. Isolat Bacillus spp. Bc 26; Bc 80 dan Bc81 dan Pseudomonad fluoresen Pf 101; Pf146 dan Pf 170 dalam bentukkombinasi sebagai perlakuan yang diisolasi dari rizosfer nilam sehat, dandiseleksi berdasarkan kemampuan antagonistik terhadap R. solanacearumsecara in vitro di laboratorium dan in planta di rumah kaca KP BalittroLaing Solok. Isolat Bacillus spp. dan Pseudomonad fluoresen tersebutdiintroduksikan ke nilam dan dibiarkan selama 1 minggu sebelum ditanam.Tanaman yang telah diperlakukan dengan isolat Bacillus spp. danPseudomonad fluoresen ditanam pada kebun nilam yang telah terinfeksioleh bakteri patogen pada bulan Mei 2006. Perlakuan yang diuji disusundalam rancangan acak kelompok (RAK) dengan 3 ulangan. Parameterpengamatan adalah perkembangan penyakit layu bakteri meliputi masainkubasi dan intensitas penyakit, pertumbuhan dan produksi tanaman.Hasil penelitian menunjukkan bahwa kombinasi isolat Bacillus spp. Bc26dan Pseudomonad fluoresen Pf101 dapat mengendalikan penyakit layubakteri nilam lebih baik dibandingkan dengan isolat Bacillus spp. Bc 26dan Pseudomonad fluoresen Pf 101 secara terpisah dan isolat Bacillus spp.dan Pseudomonad fluoresen lainnya secara kombinasi dan terpisah.Kombinasi isolat Bacillus spp. Bc 26 dan Pseudomonad fluoresen Pf 101dapat menunda masa inkubasi gejala penyakit layu bakteri dari 21 harisetelah tanam (HST) menjadi 63 HST dan menekan intensitas penyakitlayu bakteri dari 63,90% menjadi 14,67%. Di samping itu kombinasikedua isolat tersebut dapat meningkatkan pertumbuhan tanaman sepertitinggi tanaman dari 35,53 cm menjadi 52,77 cm, jumlah daun total dari32,00 daun/tanaman menjadi 104,67 daun/tanaman, jumlah tunas dari10,33 tunas/tanaman menjadi 25,33 tunas/tanaman, berat basah daun dari16,20 g/petak menjadi 81,73 g/petak dan berat kering daun dari 5,44 g/petak menjadi 27,15 g/petak. Hasil percobaan ini menunjukkan bahwakombinasi isolat Bacillus spp. Bc 26 dan Pseudomonad fluoresen Pf 101mempunyai kemampuan tertinggi dalam mengendalikan penyakit layubakteri dan meningkatkan pertumbuhan tanaman nilam di lapang.Kata kunci: Pogostemon  cablin  Benth,  penyakit  layu  bakteri,pengendalian, Bacillus spp., Pseudomonad fluorescentABSTRACTUse of Bacillus sp. and Fluorecent Pseudomonad to Control Bacterial Wilt Disease on Patchouli PlantThe study of controlling bacterial wilt disease on patchouli plant(Ralstonia solacearum) with Bacillus spp. and Fluorescent pseudomonadwas carried out in farmer field in Kajai Village, West Pasaman, WestSumatra from May to November 2006. The aims of the study were to findout the effectiveness of Bacillus spp. and Fluorescent pseudomonad forcontrolling bacterial wilt disease, and increasing plant growth andproduction. Isolates of Bacillus spp. Bc 26, Bc 80, and Bc 81, andFluorescent pseudomonad Pf 101, Pf 146 and Pf 170 in combination orseperation as treatments were isolated from the rhizosphere of healthypatchouli plant, and selected based on antagonistic activity on R.solanacearum in vitro at the laboratory and in planta at green house of KP.Balittro Laing Solok. Isolates were inoculated on patchouli plant andremained for one week before planting. The plants, treated with Bacillusspp. and Fluorescent pseudomonad isolates, were planted in the fieldinfected with pathogen bacterial in May 2006. The treatment was arrangedin a randomized block design (RBD) with three replications. Theassessment parameters were incubation period, disease intensity, plantgrowth and production of patchouli plants. The results showed thatcombination of Bacillus spp. and Fluorescent pseudomonad could controlthe bacterial wilt disease better than Bacillus spp. Bc 26 and Fluorescentpseudomonad seperately, and the other Bacillus spp. and Fluorescentpseudomonad either in combination or separation. Combination ofBacillus spp. Bc26 and Fluorescent pseudomonad Pf 101 delayed theincubation period from 21 to 63 days and decreased the disease intensity ofbacterial wilt from 63.90 to 14.67%. In addition combination of bothisolates could affect the increase of plant growth, i.e plant height from35.53 to 52.77 cm, total numbers of leaves from 32.00 to 104 leaves/plant,budding numbers from 10.33 to 25.33 budding/plant, wet weight of leavesfrom 16.20 to 81.73 g/plot, and dry weight of leaves from 5.44 to 27.15g/plot. The results of the experiment showed that Bacillus spp. Bc 26 andFluorescent pseudomonad Pf 101 isolates have the highest activity oncontrolling the bacterial wilt disease and increase the growth of patchouliplant in the field.Key words: Patchouli, Pogostemon cablin Benth, bacterial wilt disease,biological control, Bacillus spp., Fluorescent pseudomonad
PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN PSEUDOMONAD FLUORESEN NASRUN NASRUN; CHRISTANTI CHRISTANTI; TRIWIDODO ARWIYANTO; IKA MARISKA
Jurnal Penelitian Tanaman Industri Vol 11, No 1 (2005): Maret 2005
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v11n1.2005.19-24

Abstract

ABSTRAKPenelitian pengendalian penyakit layu bakteri nilam (Ralstoniasolanacearum) menggunakan pseudomonad fluoresen di kebun petaninilam Desa Situak Pasaman Barat, Sumatera Barat telah dilakukan padabulan Oktober 2003 sampai dengan Juni 2004. Penelitian ini bertujuanuntuk mendapatkan pseudomonad fluoresen yang berpotensi untukmengendalikan penyakit layu bakteri, dan meningkatkan pertumbuhandan produksi nilam. Isolat pseudomonad fluoresen Pf 63, Pf 90, Pf 91, Pf147, dan Pf 180 sebagai perlakuan diisolasi dari rizosfer nilam sehat, dandiseleksi  berdasarkan  kemampuan  antagonistik  terhadap  R.solanacearum secara in vitro di Laboratorium Bakteriologi TumbuhanFakultas Pertanian UGM. Isolat pseudomonad fluoresen tersebutdiintroduksikan ke nilam dan diadaptasikan selama 1 minggu sebelumditanam. Tanaman yang telah diperlakukan dengan isolat pseudomonadfluoresen ditanam pada kebun yang telah terinfeksi dengan patogen padabulan Oktober 2003. Percobaan disusun dalam rancangan acak kelompok(RAK) dengan 6 ulangan. Parameter pengamatan adalah masa inkubasi,intensitas penyakit, pertumbuhan tanaman dan produksi minyak nilam.Hasil penelitian menunjukkan bahwa isolat pseudomonad fluoresendapat mengendalikan penyakit layu bakteri dengan perpanjangan masainkubasi 6-52 hari dan penekanan intensitas penyakit 31,11 – 50,56%.Disamping itu isolat pseudomonad fluoresen dapat mempengaruhipeningkatan pertumbuhan tanaman yaitu tinggi tanaman (6,7 – 26,3 cm),jumlah daun (4,6 – 30,1 daun/tanaman) dan berat kering daun (24,5 –154,3 g/tanaman), dan produksi minyak nilam terutama jumlah minyak(4,8 – 22,3 ml/tanaman). Hasil percobaan ini menunjukkan bahwa isolatPf 91 mempunyai kemampuan antagonistik tertinggi dalam mengen-dalikan penyakit layu bakteri di lapangan.Kata kunci : Nilam, Pogostemon cablin Benth, penyakit layu, bakteri,pengendalian hayati, pseudomonad fluoresenABSTRACTControlling bacterial wilt disease on patchouli plant withfluorescent pseudomonadThe study of controlling bacterial wilt disease on patchouli plant(Ralstonia solanacearum) with fluorescent pseudomonad was carried outin a farmer’s field in Situak Village West Pasaman, West Sumatera fromOctober 2003 to June 2004. The aims of the study were to find out theeffectiveness of fluorescent pseudomonad for controlling bacterial wiltdisease, increasing plant growth and production. Isolates of fluorescentpseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments wereisolated from the rhizosphere of healthy patchouli plant, and selectedbased on antagonistic activity on R. solanacearum in vitro at theLaboratory of Plant Bacteriology, Faculty of Agriculture, UGM. Theisolates were inoculated on patchouli plant and adapted for one weekbefore planting. The plants treated with fluorescent pseudomonadisolates were planted in the field infected with pathogen on October2003. The experiment was arranged in a randomized block design(RBD) with six replications. The assessment parameters were incubationperiod, disease intensity, plant growth and production of patchouli plants.The results showed that fluorescent pseudomonad isolates could controlthe bacterial wilt disease and delay the incubation period 6-52 days anddecrease the disease intensity 31,11–50,56%. In addition fluorescentpseudomonad isolates could affect the increase of plant growth, i.e. plantheight ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dryweight of leaves (24,5 – 154,3 g/plant), and plant production, especiallyoil content (4,8 – 22,3 ml/plant). The results of the experiment showedthat Pf 91 isolate had the highest antagonistic activity on controlling thebacterial wilt disease on field.Key words : Patchouli, Pogostemon cablin Benth, wilt disease,bacterial, biological control, fluorescent pseudomonad
Karakteristik Morfologi Dan Fisiologi Bakteri Endofit Dan Rizobakteri Dari Tanaman Cengkeh Sehat Fina Dwimartina; Tri Joko; Triwidodo Arwiyanto
Jurnal Agro Wiralodra Vol. 4 No. 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v4i1.58

Abstract

One of the determining factors in disease control is the use of superior seeds which have good quality and quantity. The use of biological control agents that form a symbiotic mutualism in their host plants can be used as an effort to obtain quality clove seeds. Endophytic bacteria and rhizobacteria have been reported as potential biological control agents for many plant diseases because of their safety in humans and non-target organisms. The purpose of this study was to identify the physiological and morphological characteristics of endophytic and rhizobacterial isolates obtained from clove plants. A total of 46 endophytic bacterial isolates were isolated from healthy clove roots, stems, leaves and flowers. Also, 66 isolates were isolated from the soil around the rhizosphere area. Based on the results of the identification of physiological and morphological characteristics, it is evident that the endophytic and rhizobacterial bacteria tested have the same character as Bacillus spp.
KARAKTERISASI PATOGEN HAWAR DAUN BAKTERI SECARA FENOTIPIK PADA BAWANG MERAH (ALLIUM CEPA L. KELOMPOK AGGREGATUM) Asrul Asrul; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 26, No 1 (2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.313 KB)

Abstract

The research aimed at determining the types of pathogen associated with bacterial leaf blight diseases of shallots. The bacteria were isolated and characterized based on the  morphological, biochemical and physiological morphology of their colony and cell.  There were eight isolates of pathogenic bacteria from pure culture.  Generally, the isolates have Gram-negative characters, short rod-shaped cells, have peritrikus flagellum and mucoid, yellow or beige colonies, round, convex or flat forms, and is shiny. The isolates react positively to catalase, urease, levan, indole production, and tween 8 tests. They also can live at temperature between 20 – 370C, pH 5 – 7 and tolerant to NaCl content ranging from 0 – 8.5%. The isolates react negatively to oxidases, reduce nitrates, fluorescent pigments, arginine, gelatin and starch.  Based on these characteristics, the isolates found generally have a closer resemblance to the properties of P. ananatis with a similarity coefficient of 88%  than bacteria X. axonopodis pv. allii with a similarity coefficient of 78%.  The symptoms appeared in the plant leaves were wilted (water soaked), shrinking, curving down, chlorosis, necrosis, and dieback.
POTENTIAL EFFECT OF RHIZOBACTERIA Streptomyces sp. AND Bacillus sp. TO GEMINIVIRUS INFECTION AND RED CHILI (Capsicum annum L.) QUALITY PRODUCTION Azizah Ridha Ulilalbab; Sri Sulandari; Triwidodo Arwiyanto; Sedyo Hartono
Jurnal Agroteknologi Vol 14, No 1 (2023): Agustus 2023
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v14i1.24689

Abstract

The chili yellow leaf curl disease is one of the biggest obctacle in chili’s production in Indonesia. This diseases only transmitted by whitefly (Bemisia tabaci). One of the disease controlling method is by using Plant Growth Promoting Rhizobacteria (PGPR) to reduce pesticide application for the health and environment. The aim of this research is to find out the effectiveness of Streptomyces sp. and Bacillus sp. in supressing yellow leaf curl disease, plant growth and chili harvest. This research has conducted at the experimental farmer’s field in Harjobinangun, Pakem, Sleman, Special Region of Yogyakarta. Using varieties of chili Twist 42, Streptomyces isolates which from Bacteriology Laboratory collection’s with 10-8 CFU/ml and Bacillus isolates which is from Mycrobiology Laboratory collection with  10-8 CFU/ml. The result of this research showed that the treatment of combination between Bacillus sp. and Streptomyces sp. could decrease and supress disease incidence and disease intensity if it compared with others treatment. Parameters of plant growth included plant high, stem diameter, root length, root volume and number of productive branch showed that single of Streptomyces sp. and Bacillus sp. without combination were better than combination and untreated.  The single application Streptomyces sp. or Bacillus sp. could increase amount of total number and weight of fruits.
Karakterisasi Patogen Hawar Daun Bakteri Secara Fenotipik Pada Bawang Merah (Allium cepa L. Kelompok Aggregatum) Asrul Asrul; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 26 No 1 (2019): April
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/agrolandnasional.v26i1.735

Abstract

Penelitian ini bertujuan untuk menentukan jenis patogen yang berasosiasi dengan bawang merah sakit hawar daun bakteri.Penelitian dilakukan dengan metode isolasi dan karakterisasi bakteri berdasarkan sifat morfologi koloni dan sel bakteri, serta biokimiawi dan fisiologis. Hasil isolasi dan karakterisasi diperoleh 8 isolat murni bakteri patogenik yang berasosiasi dengan bawang merah sakit.Umumnya isolat memiliki sifat gram negatif, sel berbentuk batang pendek, mempunyai flagel peritrikus, koloni berwarna kuning, atau krem, berbentuk bundar, cembung, rata, mucoid dan mengkilap. Isolat bereaksi positif terhadap uji katalase, urease, levan, produksi indol, tween 80, dapat hidup pada suhu antara 20 - 37OC, ph 5 - 7 dan toleran pada kandungan NaCl 0 - 8,5%. Isolat bereaksi negatif terhadap oksidase, reduksi nitrat, pigmen fluorescen, arginine, gelatin dan pati.Berdasarkan sifat-sifat tersebut, isolat yang ditemukan umumnya memiliki kemiripan lebih dekat dengan sifat-sifat bakteri P. ananatis (koofisien kemiripan 88%) dibanding bakteri X. axonopodis pv. allii (koofisien kemiripan 78%). Gejala yang ditimbulkan berupa daun layu kebasah-basahan (water soaked), mengkerut, melekuk kebawah, klorosis, nekrosis, dan mati pucuk.
SELEKSI MUTAN ANTIBIOSIS Bacillus subtilis B315 UNTUK PENGENDALIAN Ralstonia solanacearum Pr7 Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Agrin Vol 18, No 1 (2014): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2014.18.1.213

Abstract

Bacillus subtilis B315 adalah bakteri antagonis terhadap patogen tanaman seperti Ralstonia solanacearum penyebab penyakit layu bakteri.  Salah satu mekanisme antagonisme adalah antibiosis.  Mutan antibiosis dibuat untuk membuktikan bahwa B. subtilis B315 mempunyai mekanisme antibiosis dalam mengendalikan R. solanacearum.  Tujuan penelitian adalah untuk menyeleksi mutan antibiosis B. subtilis B315 dibandingkan dengan B. subtilis B315 tipe alaminya, 2) mendeteksi sifat antibiosis dari  B. subtilis B315.  Metode yang digunakan adalah eksperimen melalui mutagenesis dengan EMS, seleksi mutan berdasarkan pengujian antibiosis, waktu generasi, pola nutrisi dan konsistensi koloni.  Sifat antibiosis dideteksi dengan ekstraksi metabolit sekunder.  Hasil penelitian menunjukkan bahwa mutagenesis dengan EMS yang optimum adalah pada waktu 274,7 menit dengan kematian maksimum 81,7%, Terdapat tiga kelompok mutan antibiosis yaitu yang tidak menghambat R. solanacearum, menghambat dengan zona hambatan 1-3 mm dan menghambat  dengan zona hambatan >3 mm. Mutan antibiosis terpilih yang kehilangan sifat  menghambat, namun waktu generasi dan pola nutrisi serta konsistensi koloni sama dengan B. subtilis B315 tipe alami adalah mutan M16.  Sifat antibiosis B. subtilis B315 ditunjukkan dengan metabolit sekunder yang diekstrak dengan metanol, menghasilkan puncak spot yang berbeda dengan mutan antibiosis M16. Kata kunci: Bacillus subtilis B315, mutan antibiosis, pengendalian, Ralstonia solanacearum  Pr7 ABSTRACT            Bacillus subtilis B315 is an antagonistic bacterium against plant pathogens such as Ralstonia solanacearum that causes bacterial wilt disease.  One of the antagonistic mechanisms is antibiosis. Antibiosis mutant is made to prove that B. subtilis B315 has an antibiosis mechanism in controlling R. solanacearum.  Aims of the research were 1) to select the B. subtilis B315 antibiosis mutant compared with B. subtilis B315 wild type, and  2) to detect antibiosis characters owned by B. subtilis B315.  The method used was an experiment through mutagenesis with EMS, mutant selection based on antibiosis test, generation time, nutrition pattern and colony consistency.  Antibiosis characters were detected by extraction of secondary metabolites.  Results of the research performed that optimal mutagenesis with EMS was at 274.7 minutes by maximum lethality of 81,7%.  There were 3 groups of antibiosis mutants i.e. not inhibiting R. solanacearum, inhibition with 1-3 mm of inhibiting zone, and inhibition with >3 mm of inhibiting zone.  The selected antibiosis mutant lost its inhibiting character, but the generation time and the nutrition pattern  and the colony consistency similar to B. subtilis B315 wild type was the M16 mutant.  Antibiosis characters of B. subtilis B315 were shown by secondary metabolites extracted with methanol to produce the peak spot that was different from the M16 antibiosis mutant. Key words: Bacillus subtilis B315, antibiosis mutant, control, Ralstonia solanacearum  Pr7 
Potensi Minyak Kayu Putih dan Minyak Serai Dapur dalam Menekan Faktor Virulensi Bakteri Xanthomonas oryzae pv. oryzae: Potensi Minyak Kayu Putih dan Minyak Serai Dapur dalam Menekan Faktor Virulensi Bakteri Xanthomonas oryzae pv. oryzae Alifia Nur Ayusma; Triwidodo Arwiyanto; Widiastuti, Ani
Jurnal Fitopatologi Indonesia Vol. 20 No. 4 (2024): Juli 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.4.195-203

Abstract

Penyakit hawar daun yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo), merupakan salah satu jenis penyakit yang penting pada tanaman padi dan dapat menyebabkan kehilangan hasil mencapai 10-50%. Upaya pengendalian penyakit hawar daun bakteri pada tanaman padi dengan minyak kayu putih dan minyak serai dapur belum banyak dilaporkan. Tujuan penelitian ialah mengevaluasi daya hambat dan penekanan faktor virulensi dari minyak kayu putih dan minyak serai dapur terhadap Xoo kode isolat BaK_2 secara in vitro. Pengujian daya hambat dilakukan dengan metode double layer. Pengujian faktor virulensi dilakukan terhadap pembentukan biofilm, pembentukan eksopolisakarida (EPS), serta motilitas X. oryzae pv. oryzae. Hasil penelitian menunjukkan bahwa minyak kayu putih maupun minyak serai dapur dapat menghambat pertumbuhan X. oryzae pv. oryzae secara in vitro. Potensi minimum inhibitory concentration (MIC) pada masing-masing yaitu 15% pada minyak kayu putih dan 5% pada minyak serai dapur. Faktor virulensi pada tiga pengujian, menunjukkan bahwa minyak kayu putih dan minyak serai dapur tidak berpengaruh signifikan terhadap pembentukan biofilm, namun dapat menurunkan pembentukan EPS dan mampu membuat pergerakan bakteri minimum inhibitory concentration menjadi terbatas baik secara swimming motility maupun secara twitching motility.
Co-Authors . Supriyanto Achmadi Priyatmojo Alifia Nur Ayusma Anggita Rahmawati Ani Widiastuti, Ani Ari Astuti Arif Wibowo Asrul Asrul Asrul Asrul Asrul Azizah Ridha Ulilalbab Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hendro Sunarminto Bambang Trianom CHRISNAWATI CHRISNAWATI CHRISTANTI CHRISTANTI Christanti Sumardiyono Didik Indradewa Didik Indradewa Djati Widhityarini Dwimartina, Fina Elizabeth Handini Gembong Dalmadiyo Heru Adi Djatmiko I. Hartana I. Hartana IKA MARISKA Irpawa, Dea Musytari Intan Isna Maulida JAKA WIDADA Jaka Widada Jaka Widada Kharis Haryono Lisa Navitasari Murti, Rudi Hari NASRUN NASRUN NASRUN NASRUN Nur Prihatiningsih Putri Laeshita Riska Awalia Putri Rudi Hari Murti Saputra, Rachmad SEDYO HARTONO Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sudarmadi Sudarmadi Suhartiningsih Dwi Nurcahyanti Tati Suharti Tati Suharti Toekidjo Martoredjo Toekidjo Martoredjo Tri Joko Tri Joko Tri Joko Tri Joko Tri Retno Widyastuti Wulandari, Rani Agustina Y. M. S. Maryudani Yenny WURYANDARI