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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agroland : Jurnal Ilmu-Ilmu Pertanian Berkala Ilmiah Pertanian Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Tanaman Industri Jurnal Ilmu-Ilmu Pertanian Indonesia JURNAL AGROTEKNOLOGI Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian Indonesian Journal of Agricultural Research Jurnal Agro Wiralodra Jurnal Penelitian Tanaman Industri (Littri) Agroland: Jurnal Ilmu-ilmu Pertanian
Triwidodo Arwiyanto
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary

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Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro Saputra, Rachmad; Arwiyanto, Triwidodo; Wibowo, Arif
Indonesian Journal of Agricultural Research Vol. 2 No. 3 (2019): InJAR, Vol. 2, No. 3, November 2019
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1041.563 KB) | DOI: 10.32734/injar.v2i3.1389

Abstract

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.
The characterization, pathotype distribution and genetic diversity of Xanthomonas oryzae pv. oryzae in rice production centers of the Special Region of Yogyakarta, Indonesia Irpawa, Dea Musytari Intan; Arwiyanto, Triwidodo; Sulandari, Sri
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224262-273

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a pathogenic bacterium that induces bacterial leaf blight in rice. This disease is challenging to treat due to the presence of multiple pathotypes that can harm the plants. Characterizing and determining the distribution of pathotypes and genetic diversity of Xoo in the Special Region of Yogyakarta is essential for evaluating the appropriate approach to managing rice leaf blight in different districts of Yogyakarta. This study aimed to ascertain the characterization, distribution of pathotypes, and genetic diversity of Xoo in the Special Region of Yogyakarta. This study involved the isolation and characterization of Xoo from various rice production centers in Yogyakarta. The distribution of Xoo pathotypes was determined using five differential varieties (Tetep (4251), PB 5 (4827), Java 14 (11022), Kencana Bali (4477), and Kuntulan (1529)). Pathogenicity testing was conducted on six common varieties used by Yogyakarta farmers (IR64, Ciherang, C4, Mekongga, Menthik Wangi, and Inpari 42). Additionally, molecular characterization of Xoo was performed. The bacterial leaf blight that affects rice plants in the Yogyakarta region, caused by Xoo, is identified by yellow circular colonies. It exhibits a negative Gram staining response, positive catalase activity, negative oxidase activity, and does not hydrolyse starch. The Xoo pathotypes identified in Yogyakarta are IV, VIII, and XI. All six prevalent cultivars utilized by farmers in Yogyakarta are susceptible to Xoo. Out of each pathotype, four isolates were chosen, and they were divided into two distinct groups based on the DNA banding pattern they formed. Among these isolates, three had the lowest base sequence at 200 bp, while one isolate had a different DNA banding pattern with the lowest base sequence between 250–300 bp.
Co-Authors . Supriyanto Achmadi Priyatmojo Alifia Nur Ayusma Anggita Rahmawati Ani Widiastuti, Ani Ari Astuti Arif Wibowo Asrul Asrul Asrul Asrul Asrul Azizah Ridha Ulilalbab Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hendro Sunarminto Bambang Trianom CHRISNAWATI CHRISNAWATI CHRISTANTI CHRISTANTI Christanti Sumardiyono Didik Indradewa Didik Indradewa Djati Widhityarini Dwimartina, Fina Elizabeth Handini Gembong Dalmadiyo Heru Adi Djatmiko I. Hartana I. Hartana IKA MARISKA Irpawa, Dea Musytari Intan Isna Maulida JAKA WIDADA Jaka Widada Jaka Widada Kharis Haryono Lisa Navitasari Murti, Rudi Hari NASRUN NASRUN NASRUN NASRUN Nur Prihatiningsih Putri Laeshita Riska Awalia Putri Rudi Hari Murti Saputra, Rachmad SEDYO HARTONO Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sudarmadi Sudarmadi Suhartiningsih Dwi Nurcahyanti Tati Suharti Tati Suharti Toekidjo Martoredjo Toekidjo Martoredjo Tri Joko Tri Joko Tri Joko Tri Joko Tri Retno Widyastuti Wulandari, Rani Agustina Y. M. S. Maryudani Yenny WURYANDARI