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All Journal HAYATI Journal of Biosciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Jurnal Hortikultura Jurnal Matematika & Sains AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology Acta Pharmaceutica Indonesia BERITA BIOLOGI Jurnal Biodjati Jurnal Mikologi Indonesia Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Asian Journal of Agriculture Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian 3BIO: Journal of Biological Science, Technology and Management Makara Journal of Science Archipelago: Jurnal Pengabdian Kepada Masyarakat Iain Ternate Jurnal Kesehatan Bakti Tunas Husada Biodiversitas Journal of Biological Diversity
I Nyoman Pugeg Aryantha
Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Other

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ISOLASI DAN IDENTIFIKASI BAKTERI PENAMBAT NITROGEN UNTUK PEMBUATAN BIOFERTILIZER asrul, asrul; Pugeg Aryantha, I Nyoman
Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Vol 15 No 1 (2021): Mei 2021
Publisher : Universitas Islam Balitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35457/viabel.v15i1.1386

Abstract

Nitrogen is a macro nutrient needed by plants. Generally, people use inorganic fertilizers to fulfill nitrogen nutrients in plants. The problem then is, the continuous use of synthetic nitrogen fertilizers has a direct negative impact on the soil and a derivative impact on human health. The use of microorganisms, in this case bacteria, to provide nitrogen to plants can be done by isolating it and making it a biological fertilizer agent. Nitrogen fixing bacteria was isolated on the land of the oil palm plantation of PT Astra Agro Lestari. The isolated nitrogen-fixing bacteria were then tested quantitatively for their ability to fix nitrogen. The bacteria with the highest nitrogen fixing ability were then identified by sequencing their DNA nucleotide bases so that the bacterial strains were identified. The result is that there are 13 bacteria that are able to fix nitrogen with the codes J1, J3, Q5, L1, L11, J31, D1, M6, M5, R1, P2, J4 and C7. The quantitative test shows that bacteria with code D1 are the best at fixing nitrogen in the form of NH4, namely 0.27 ppm. The results of D1 bacterial DNA nucleotide base sequencing showed that the putitive Bacillus aerius strain 24K with identical values ​​and query cover reach
Antibacterial activity of mycelial extract from a local fungus, Sclerotium rolfsii Zahra, Muhandinni; Aryantha, I Nyoman Pugeg; Sukrasno, Sukrasno; Suhardi, Veronica Sri Harjati
Indonesian Journal of Biotechnology Vol 30, No 3 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.108408

Abstract

Mycelium‐to‐sclerotium differentiation in fungi involves not only morphological but also biochemical changes throughout the process, which may contribute to their persistence and be a possible source of bioactive compounds. This study aims to evaluate the antibacterial activity and identify the bioactive compound in the local isolate Sclerotium rolfsii. Fungal culture was grown in media containing potato extract (20 g/L), dextrose (20 g/L), and peptone (5 g/L) for 27 days under static conditions at room temperature. Mycelium, sclerotium and filtrate were collected every three days and extracted with methanol, followed by evaporation and antibacterial screening. Significant activity was observed in day three of mycelial extract, which showed morphology of initial sclerotium formation (MIC 0.39 mg/mL) against B. subtilis and E. coli. An improved extraction method (sequential extraction) was employed for mycelial sample on the third day. N‐hexane and ethyl acetate extracts exhibited stronger activities (0.20 mg/mL). Ergosterol was identified after TLC‐bioautography, radial chromatography, and NMR elucidation analysis. S. rolfsii mycelium (third day‐sclerotial initiation) was found to contain ergosterol, demonstrating strong defense against bacteria, and possibly related to sclerotium‐differentiation metabolites. These findings may pave the way for more extensive studies of sclerotium differentiation as an interesting phenomenon of fungal development and bioactive compound origins.
Laboratory screening of three local entomopathogenic fungal isolates against third-instar Spodoptera frugiperda LILIAN RIZKIE; TJANDRA ANGGRAENI; I NYOMAN PUGEG ARYANTHA; AGUS SUSANTO
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100181

Abstract

Abstract. Rizkie L, Anggraeni T, Aryantha INP, Susanto A. 2026. Laboratory screening of three local entomopathogenic fungal isolates against third-instar Spodoptera frugiperda. Asian J Agric 10: g100181. https://doi.org/10.13057/asianjagric/g100181. The fall armyworm (Spodoptera frugiperda) is an important pest of maize in tropical production systems, including Indonesia. In the field, control of this pest still relies mainly on synthetic insecticides, making the evaluation of local biological control agents necessary. This study tested three local entomopathogenic fungal isolates, morphologically assigned to Beauveria, Metarhizium, and Lecanicillium, against third-instar S. frugiperda larvae at 10⁶-10⁹ conidia mL-¹. Day-7 mortality and sublethal responses were analyzed with generalized linear models, while LT and LC values were estimated by probit analysis. At 10⁹ conidia mL-¹, mortality reached 85.0% for the Beauveria isolate and 90.0% for the Metarhizium isolate, compared with 63.3% for the Lecanicillium isolate. LT₅₀ values at this concentration were 2.75, 2.56, and 5.08 days, respectively. LC estimates outside the tested concentration range were treated as assay-based comparative indices, not as absolute toxicity thresholds. After 48 h of sublethal exposure, relative growth rate, relative consumption rate, and efficiency of conversion of ingested food were reduced, especially in the Beauveria and Metarhizium treatments. Overall, the Beauveria and Metarhizium isolates showed stronger laboratory performance against S. frugiperda larvae than the Lecanicillium isolate. Molecular confirmation and greenhouse or field validation are still required before practical application can be considered.
Optimization of Ganoderma lucidum Mycelium Production for Biomaterial Production on Solid Medium with Bamboo and Empty Palm Oil Bunch Variation Mustika Dewi; Rakha Pradipa Bhagaskara Susanto; I Nyoman Pugeg Aryantha
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 2 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.2.5

Abstract

The chemical composition of sengon wood is ideal for the nutritional needs of fungi, particularly wood fungi. Fungi obtain their energy by degrading the lignocellulosic components (cellulose, hemicellulose, and lignin) found in wood. Although sengon is an excellent substrate, its availability is limited, and its price continues to rise. Potential alternative cultivation media include bamboo and oil palm, which are the natural habitats of Ganoderma lucidum. This study investigates the potential of bamboo sawdust and shredded empty palm oil fruit bunches (EFB) as alternative substrates for growing G.lucidum. Currently, G. lucidum is cultivated using a cereal-based medium with sengon sawdust waste. This study examines the growth of G. lucidum in media containing bamboo sawdust and EFB as an alternative media derived from organic waste. The experiment involves three stages: inoculating G. lucidum into media with varying concentrations of bamboo sawdust and EFB, adapting the fungus to complex media with different concentrations of these materials, and finally substituting sengon sawdust with bamboo sawdust or EFB in a control medium to observe growth parameters. The results showed that most media supplemented with bamboo sawdust and EFB, except for the 2% and 4% bamboo sawdust concentrations in the first stage, significantly increased growth (p < 0.05). However, in the second stage, only certain concentrations (25% bamboo sawdust and 50% EFB) achieved the same growth rate as the control. In the third stage, G. lucidum in media modified with bamboo showed significantly faster growth, although the loss of organic matter was not significantly different. Although bamboo and EFB did not increase the biomass production of G. lucidum mycelium both showed potential as alternatives. These findings indicate that these materials are suitable for use as a growth medium for G. lucidum.
Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum Indah Juwita Sari; Indria Wahyuni; Rida Oktorida Khastini; Ewi Awaliyati; Andriana Susilowati; Enggar Utari; I Nyoman Pugeg Aryantha
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13191

Abstract

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.
Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus Dewi Peti Virgianti; Desi Natalia; I Nyoman Pugeg Aryantha
Biodiversitas Journal of Biological Diversity Vol. 21 No. 4 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210449

Abstract

Abstract. Virgianti DP, Natalia D, Aryantha INP. 2020. Short Communication: New record of Stenotrophomonas sp. as endosymbiont bacteria in Rhizopus microsporus. Biodiversitas 21: 1678-1685. Rhizopus sp. are closely related to humankind in many aspects of human life. Several species of Rhizopus are important in food, agriculture, and health industries. Rhizopus microsporus has endosymbiont bacteria that has been identified as Burkholderia sp. This study aimed to identify the endosymbiont bacteria in R. microsporus isolated from Moringa oleifera Lam leaves. Ring technique, simple method of agar heap technique and antibiotic technique, was conducted to purify the hyphae and to eliminate ectosymbiont and contaminant bacteria outside the hyphae. The presence of endosymbiont bacteria in R. microsporus was determined by using LIVE/DEAD® BacLight Bacterial Viability Kits L13152 and the Fluorescent in Situ Hybridization (FISH) method using EUB338 probes. It was suggested that the culturable endosymbiont bacteria was identified as Stenotrophomonas sp. based on the phylogenetic tree using the 16S rDNA
Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem Rahayu Fitriani Wangsa Putrie; I Nyoman Pugeg Aryantha; IRIAWATI IRIAWATI; Sarjiya Antonius
Biodiversitas Journal of Biological Diversity Vol. 21 No. 7 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210728

Abstract

Abstract. Putrie RFW, Aryantha INP, Iriawati, Antonius S. 2020. Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem. Biodiversitas 21: 3084-3093. The natural environment majorly influences plant microbiome diversity, in terms of endophytic and rhizobacteria. Pineapple (Ananas comosus L. Merr) has been identified as one of host plants with a capacity to grow in semiarid ecosystems. The intrinsic microbial symbiont composition is recognized in a specific ecological niche. The scientific information available is limited to the inoculants' ability to reduce plant stress, although the diversity in a semiarid ecosystem has not been studied. The aim of this research is to investigate the diversity of endophytic and rhizosphere bacteria obtained from pineapple in a semiarid ecosystem. A total of 117 culturable microbial isolates, dominated by Gram-positive bacteria were obtained from pineapple (88) and rhizosphere (29). The metagenomic analysis showed higher diversity and abundance in rhizospheres compared to the endophytes. Actinobacterium was identified as the most populated colony, while Proteobacterium was more prominent in the host plant. The Bacillus genera were recognized as the dominant symbiont genus. Therefore, the culturable isolates were further developed as candidate inoculants for microbe databases. This is expected to be useful in broadening theoretical knowledge.
Co-Authors Adhityo Wicaksono Adi Pancoro Adi Pancoro AGUS SUSANTO ANDINI WARIH WEDARINGTYAS Andriana Susilowati Ari Widodo asrul, asrul asrul Darah Ibrahim DEMI TRISTAN DJAJADI Desi Natalia Dewi Peti Virgianti Dwiantara, Wahyu Setyaji Enggar Utari Ewi Awaliyati FENNY MARTHA DWIVANY HERI SETIADI Indah Juwita Sari Indria Wahyuni IRIAWATI IRIAWATI Irmanida Batubara Karlina, Yenni Khusnul, Khusnul KUSUMA, ALI BUDHI Latifah Kosim Darusman LILIAN RIZKIE LISTYA UTAMI KARMAWAN Mamat Kandar Mashuri Waite Meti Kusmiati Mustafa, Fransisca Astrid Mustika Dewi Nampiah Sukarno Nuryani Rustaman Panca Jarot Santoso Panca Jarot Santoso Rahayu Fitriani Wangsa Putrie Rahmat Ariffudin Rakha Pradipa Bhagaskara Susanto Reza Raihandhany Rianti Nurpalah Rida Oktorida Khastini Rizkita Rachmi Esyanti Rohani Cinta Badia Ginting Sarjiya Antonius Sihegiko Kanaya Sony Suhandono SONY SUHANDONO Suhardi, Veronica Sri Harjati Sukrasno Sukrasno Sukrasno Sukrasno Sukrasno, Sukrasno Susanti Wulandari Syarifah A. Rashid TATI KRISTIANTI TJANDRA ANGGRAENI Tutus Gusdinar Kartawinata Utut Widyastuti Wahyu Setyaji Dwiantara WINDI SILVANI JEMSI Yenni Karlina Yuniar Mulyani Zahra, Muhandinni