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All Journal Buletin Peternakan JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Ilmu Ternak Veteriner agriTECH Indonesian Journal of Biotechnology Widyariset Livestock and Animal Research Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Widyariset Journal of Tourism Sustainability
Zaenal Bachruddin
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281
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Pengaruh Inokulasi Lactobacillus plantarum dan Saccharomyces cerevisiae terhadap Fermentasi dan Kecernaan In Vitro Silase Kulit Buah Kakao Muhammad Askari Zakariah; Ristianto Utomo; Zaenal Bachruddin
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9294

Abstract

The objective of this study was to identify the effect of L. plantarum and S. cerevisiae mixed culture inoculation into cocoa pods silage on chemical composition and in vitro digestibility. The four treatments were: 1 kg freshly harvested cocoa pods without inoculants as control (K); K + L. plantarum (KLp); K + S. cerevisiae (KSc); and K + L. plantarum and S. cerevisiae mixture (KLp+Sc) 0.1% dry matter, Cassava meal were added in all treatments. Each treatment was replicated 3 times, and then fermented for 21 days. Parameters observed in current study were gas test production, ruminal fermentation parameter, and in vitro digestibility. The collected data were analyzed by one-way analysis of variance and followed by Duncan’s new Multiple Range Test for data with significant differences. Result showed that the mixed culture Lp+Sc inoculation increased (P<0.05) chemical quality of cocoa pods by reducing fibre fraction and increase NFE contents, increased degradation rate, degradation theory, reduced rumen pH, and propionate acid production, without affecting acetate to propionate ratio, microbial protein synthesis, and digestibility of cocoa pod silage.
THE EFFECT OF MOLASSES, Lactobacillus plantarum, Trichoderma viride, AND ITS MIXTURES ADDITION ON THE QUALITY OF TOTAL MIXED FORAGE SILAGE Vian Dwi Chalisty; Ristianto Utomo; Zaenal Bachruddin
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.17337

Abstract

The objective of this research was to determine the effect of molasses, Lactobacillus plantarum, Trichoderma viride, and its mixtures addition on the quality of total mixed forage silage. Molasses was added 4% (w/w), L. plantarum 0,1% (v/w), and T. viride 0,1% (v/w). Each treatment was made 3 replication and then fermented for 21 days anaerobically. Variables measured were physical, chemical, and biological quality includes color, odor, texture, fungi, pH, lactic acid content, and ammonia. Data were analyzed using the analyses of variance Completely Randomized Designs. If there was significant among the treatment, it followed by a test of the average between two treatments with Duncan’s New Multiple Range Test. Test the quality of physical showed a yellowish green color, smell sour, dense texture, and the presence of the fungus little / no fungi. The addition of molasses alone or a mixture of molasses and L. plantarum/T. viride lowered pH and ammonia (P <0.05), while the lactic acid content increased (P <0.05). The addition of molasses or water-soluble carbohydrate is a must to produce good quality total mixed forage silage that shown with pH 3.60, lactic acid content 4.28% DM, ammonia content 0.43% DM.
EFFECT OF LIGNOSELULOLITIC FUNGUS TO ENZIMATIC ACTIVITY, FIBER FRCTION, AND DIGESTIBILITY ON FERMENTATION PROCESS OF COCOA POD Engkus Ainul Yakin; Zaenal Bachruddin; Ristianto Utomo; Ria Millati
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i3.22657

Abstract

The study was conducted to determine the enzyme activity, fiber fraction and digestibility in the fermentation process of cocoa pod. The substrate was used the cocoa pod while the fungi used Phanerochaete chrysosporium, Pleurotus ostreatus and Schizophyllum commune. Preparation of cocoa pod was chopped, finely ground and then dried. Preparation of fungi by growing fungi in liquid medium. Research methodology was the fermentation conducted with different fungi used four treatments and five replications. T1 = fermentation of cocoa pod without fungi addition, T2 = fermentation of cocoa pod with P. chrysosporium addition, T3 = fermentation of cocoa pod with  P. ostreatus addition, and T4 = fermentation of cocoa pod with S. commune. Fermentation used Erlenmeyer 250 ml and weight of cocoa pod was 100 gram. Fungi added on 5% from substrat weight bassis dry matter. Variables observed was enzyme activity, fiber fraction and digestibility . This study was designed using research design completely randomized design with a unidirectional pattern analysis of variance. Significant variables followed Duncan’s multiple range test. The results showed fermentation used P. chrysosporium has highest lignin peroxidase enzyme activity of 0.52±0.04 U/mL and mangan peroxidase 0.06±0.00 U/mL, neutral detergent fiber 75,54±0,41%, acid detergent fiber 68,10±0,30%, lignin 26,86±0,19%, cellulose 27,17±0,25%, hemicellulose 6,77±0,52%, dry matter digestibility 69,70±0,43% and organic matter digestibility 69,59±1,03%. The conclusion from this research that the fermentation by using fungi P. chrysosporium addition has the best result to degradated lignin. 
THE EFFECT OF LACTIC ACID BACTERIA AND DIFFERENT LEVEL OF CARBOHYDRATE SOURCES ADDITION ON TOFU WASTE INDUSTRY FERMENTATION Zaenal Bachruddin
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i3.23677

Abstract

A The purpose of this research was to figure out the affinity value of soluble carbohydrates from pollard with lactic acid bacteria (LAB) and its effect on tofu waste silage. Lactic acid bacteria (LAB) isolates used as inoculum were commerce LAB isolates (B13-1) and yolk LAB isolates (K6-3). The selected of LAB isolates used in the tofu waste silage were tofu waste (TW) and pollard (P) with different proportion (70:30), (60:40), and (55:45). The result showed that the addition of soluble carbohydrates sources in the tofu waste silage did not affect lactic acid and pH value. However LAB addition significantly increased lactic acid (P<0.05). The TW:P proportion (55:45) produced the highest lactic acid concentration with the value of 3.54%DM with pH value 3.90. Proportion of TW:P gave significantly different effect on dry matter (DM) and organic matter (OM) (P<0.01). Meanwhile, LAB addition presented significant difference in declining of dry matter percentage (P<0.01) but it showed non-significant effect on organic matter percentage from the tofu curd silage. It can be concluded that pollard addition with the value of 45% shows that tofu waste silage has the highest lactic acid concentration and ideal pH value.
The Potential of Hydrolysate from Rabbit Meat Protein as an Angiotensin Converting Enzyme Inhibitor Edy Permadi; Jamhari Jamhari; Edi Suryanto; Zaenal Bachruddin; Yuny Erwanto
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.31495

Abstract

This research aimed to investigate the rabbit meat hydrolysate potential as an angiotensin-converting enzyme (ACE) inhibitor. Indonesian local rabbit meats were used in this study. The research was conducted in Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, from August 2016 to February 2017. The local rabbit meats were hydrolyzed by pepsin, trypsin, and pancreatic. The obtained hydrolysates were then analyzed to identify the water-soluble protein content. The molecular weight of the hydrolysates were also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The ACE inhibitory properties of the hydrolysates were analyzed in vitro. The results showed that pepsin, trypsin, and pancreatic hydrolysis showed a significant effect on the water-soluble protein content of rabbit meat (p<0.05). The water-soluble protein of rabbit meat hydrolysed by pepsin, trypsin, and pancreatic were 9.41, 7.66, and 9.75 mg/mL respectively. The molecular weight of the rabbit meat hydrolysate were increased from 10 to 43 kDa; 17 to 43 kDa; and 10 to 43 kDa, after hydrolysed by by pepsin, trypsin, and pancreatic respectively. Furthermore, the ACE inhibitory properties ) of the hydrolysed rabbit meat by pepsin, trypsin, and pancreatic were 439, 170, and 380 μg/mL, respectively. The rabbit meat hydrolysate showed a potential to be ACE inhibitor after hydrolyzed with pepsin, trypsin and pancreatic. Moreover, it also showed a promising potential to be used as bioactive components in different pharmaceutical applications. The highest ACE inhibitory capability was showed on trypsin hydrolysis with the total of 65.45% and 170 μg/mL ACE inhibition
Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility Rahma Fitriastuti; Lies Mira Yusiati; Budi Prasetyo Widyobroto; Zaenal Bachruddin; Chusnul Hanim
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.35591

Abstract

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.
Bacteriocin Activity of Lactic Acid Bacteria Isolated from Rumen Fluid of Thin Tail Sheep Okti Widayati; Zaenal Bachruddin; Chusnul Hanim; Lies Mira Yusiati; Nafiatul Umami
Buletin Peternakan Vol 43, No 3 (2019): BULETIN PETERNAKAN VOL. 43 (3) AUGUST 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i3.36837

Abstract

The objective of this study was to determine the activity and the stability of bacteriocin from lactic acid bacteria (BAL) isolated from rumen fluid of thin-tail sheep under the temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). Lactic acid bacteria obtained by isolation, selection, and identification of thin-tailed sheep rumen fluid were used for bacteriocin production. The crude bacteriocin was partially purified using 70% ammonium sulfate, then was dialysis for 12 hours. The obtained bacteriocin then tested its inhibitory activity against E.coli (representing Gram-negative) and S. aureus (representing Gram-positive) under temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). The data of bacteriocin activity based on pH, temperature, and the length of storage were analyzed with factorial, then when there was a significant difference of variable because treatment was continued with Duncan's Multiple Range Test (DMRT) test. The results showed that the bacteriocin activity of the three types of BAL against S.aureus is greater than E.coli. The highest activity was shown in pH 3, while the lowest activity was shown at pH 10 (P<0.01). The highest activity was shown at a heating temperature of 100°C, while the lowest activity was shown at a heating temperature of 80°C (P<0.01). The activity of bacteriocin produced by BAL 0 A, BAL 1 A, and BAL 4 C tended to be stable to the heating temperature of 80, 100, and 121°C but decreased with increasing pH value (pH 3, 7, and 10). The best of bacteriocin activity was found at pH 3 (acid), heating at 100°C, and stored at -8°C for 14 days.
Pengembangan Isolat Bakteri Asam Laktat AST 6 Dari Saluran Pencernaan Ikan Sebagai Agensia Probiotik Astuti Astuti; Zaenal Bachruddin; Supadmo Supadmo; Eni Harmayani
Jurnal Pendidikan Matematika dan Sains No 2 (2006): Jurnal Pendidikan Matematika dan Sains Tahun XI
Publisher : Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jpms.v11i2.12432

Abstract

Penelitian ini bertujuan untuk mengetahui pengembangan isolat bakteri asam laktat AST 6 dari saluran pencernaan ikan sebagai agensia probiotik dan juga untuk mengetahui pengaruh penggunaan inokulum mikrobia Bakteri Asam Laktat AST 6 terhadap daya tahan medium yang mengandung garam empedu. Garam empedu dengan konsentrasi 0%; 0,05%; 0,10%; 0,15%; dan 0,20% ditambahkan dalam medium MRS yang ditumbuhi BAL AST 6 dengan masing-masing tiga ulangan. Pertumbuhan BAL dievaluasi setiap jam berdasarkan perubahan densitas, penurunan pH maupun kecepatan tumbuh spesifiknya. Produksi asam laktat diukur pada puncak fase eksponensial. Data yang diperoleh dianalisis menggunakan analisis variansi dengan Rancangan Acak Lengkap (RAL) pola searah, dan jika terdapat perbedaan di antara reratanya maka dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa isolat BAL AST 6 dari saluran pencernaan ikan toleran terhadap garam empedu sampai pada konsentrasi 0,15%. Penambahan garam empedu 0,15% dan 0,20% secara nyata (p0,05) menurunkan densitas biomassa pada jam ke-22 dari 0,982 (tanpa garam empedu) menjadi 0,802 dan 0,808. Kecepatan tumbuh spesifik mulai menurun secara berturut-turut dari 0,0974 menjadi 0,0838; 0,0765; 0,0510; dan 0,0589 g BK sel/jam. Garam empedu berturut-turut secara nyata (p0,05) juga menurunkan produksi asam laktat pada jam ke-21 dari 0,645 menjadi 0,477; 0,361; 0,376; dan 0,352 mg/ml. Penurunan pH secara nyata (p0,05) terjadi pada jam ke-22 pada semua konsentrasi garam empedu. Kesimpulannya bahwa isolat BAL AST 6 dari saluran pencernaan ikan masih mampu tumbuh sampai konsentrasi garam empedu 0,15% dan dapat berpotensi sebagai agensia probiotik.Kata kunci: bakteri asam laktat, garam empedu, probiotik, densitas, pH, kecepatan tumbuh spesifik, asam laktat
EFFECT OF MIXING THE CALF FECES WITH COASTAL SANDYSOIL ON THE GROWTH OF CORN PLANT (Zea mays) Agung Prabowo; Soemitro Padmowijoto; Zaenal Bachruddin; Abdul Syukur
Widyariset Vol 14, No 2 (2011): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.375 KB) | DOI: 10.14203/widyariset.14.2.2011.305-312

Abstract

This research was intended to determine the effect of combination calf feces and coastal sandy-land on the growth of corn plant. This experiment was arranged in a 2x3x2 factorial pattern of completely randomized design. First factor was calf feces of with and without microbe treatment, second factor was three levels of feces incubation which were 0 day (I-0), 20 days (I-20), and 40 days (I-40), and third factor was fecal dosages of 15 ton/ha (D15) and 30 ton/ha (D30). Parameters of plant height, dry, and wet weight of roots and vegetative, chemical and physical properties of mixture of calf feces and coastal sandy-land were observed. The plant height was observed weekly. There was a signifi cant difference (P<0.05) on combination of feces without and with microbe treatment,fecal incubation time and dosage on the plant height. There was a tendency of increasing fecal dosage and fecal incubation time on the increase of plant height. This study suggests that calf feces could improve the corn plant growth in coastal sandy-land.
In Vitro Stability of Phytase from Recombinant Bacteria E. Coli BL21 (DE3) EAS1-AMP Adi M.P. Nuhriawangsa; Sajidan Sajidan; Zaenal Bachruddin; Ali Wibowo
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 9, No 1 (2011): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.v9i1.4742

Abstract

The objective of the research was to inquire the Km, Vm, activity, intracellular phytase stability exposed to pH variation, temperature variation and protease (pepsin and pancreas) in vitro. The phytase was produced from recombinant bacteria E. coli BL21(DE3) EAS1-AMP using 1.5 mM IPTG as inducer. Intracellular enzyme was extracted via freeze shock and centrifugation. Pure enzyme was acquired through NI-NTA agarose column. The enzyme was then tested for Km, Vm, phytase activity and stability against pH, temperature and protease. Treatment levels for stability against protease were P0: without protease, P1: addition of pepsin, P2: addition of pepsin and pancreas, and the data were statistically analyzed using analysis of variance of one-way Completely Randomized Design. Crude intracellular phytase had Vm 6.39 υM/sec, Km 34.82 υM, and 277 units activity. Intracellular phytas was stable at pH 4–6 and 0–550 C. Protease level influenced the activity of intracellular phytase (P<0.05). Intracellular phytase was stable against pepsin but not pancreas.
Co-Authors Abdul Syukur Abdul Syukur Abdul Syukur Adi M.P. Nuhriawangsa Agung Prabowo Agung Prabowo Agung Prabowo Ali Mursyid Wahyu Mulyono Ali Wibowo Astuti Astuti Bambang Suwignyo Budi Prasetyo Widyobroto Chusnul Hanim Dewi Haryani Susilastuti, Dewi Haryani Edy Permadi Engkus Ainul Yakin Eni Harmayani Eni Harmayani Fathur Rahman Galih Pawening Hari Hartadi Harwanto Harwanto Harwanto Harwanto Jamhari Jamhari Lies Mira Yusiati Made Kutanegara, Pande Mohamad Soejono Mugiono (Mugiono) Muhammad Askari Zakariah Muhammad Nur Cahyanto N. Nofisulastri Nafiatul Umami Nofisulastri N, Nofisulastri Pawening, Galih Rahma Fitriastuti Ria Millati Risa Antari Ristanto Utomo Ristianto Utomo Ristianto Utomo Ristianto Utomo Sajidan Sajidan Soemitro Padmowijoto Soemitro Padmowijoto Soemitro Padmowijoto Supadmo Supadmo Suryanto, Edi Vian Dwi Chalisty Wahidin Teguh Sasongko Widayati, Okti Yuny Erwanto Zuprizal (Zuprizal)