Article Per Year (5 Year)
p-Index From 2021 - 2026
2.533
P-Index
This Author published in this journals
All Journal AL KAUNIYAH Alchemy : Journal of Chemistry Teknosains: Media Informasi Sains dan Teknologi Jurnal Agro Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research Walisongo Journal of Chemistry Lontara Abdimas : Jurnal Pengabdian kepada Masyarakat Automotive Experiences Molekul: Jurnal Ilmiah Kimia
Maswati Baharuddin
Universitas Islam Negeri Alauddin Makassar
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Nursing Physics Public Health

Published : 35 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 35 Documents
Search

 1   2   3   4 
Potensi Energi Listrik dari Microbial Fuel Cell (MFC) Menggunakan Substrat Molase dan Bakteri Pseudomonas sp. Ilmi Wahyuni; Heri Heriyono; Aisyah Aisyah; Maswati Baharuddin; Iswadi Ibrahim Patunrengi
ALCHEMY:Journal of Chemistry Vol 10, No 1 (2022): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v10i1.12154

Abstract

Sugarcane molasses have been reported as potential biomass to produce electricity from its metabolic processes through the microbial fuel cell (MFC) system. However, it is important to improve electrical generation by using both appropriate and readily available substrates and microorganisms. This study aimed to determine the current and potential difference as well as the power density generated from the metabolic process of the molasses substrate. A dual-chamber of MFC was arranged in series to generate electrical current. The anode chamber contained a mixture of molasses substrate, potassium phosphate buffer pH 7, and Pseudomonas sp. The cathode chamber contained 0.2 M KMnO4 electrolyte solution. Measurement of current and potential differences was conducted every 4 hours for 36 hours. The results showed that the maximum current, potential difference, and power density were 1656 mV, 1582 µA, and 1794.37 mW/m2, respectively.Keywords: dual chamber, microbial fuel cell, molasses, Pseudomonas sp.  Molase telah banyak dilaporkan sebagai salah satu sumber energi listrik yang potensial dengan menggunakan sistem microbial fuel cell (MFC). Namun demikian, produksi energi listriknya perlu ditingkatkan dengan menggunakan substrat dan mikroorganisme yang tepat dan mudah diperoleh. Tujuan dari penelitian ini adalah untuk mengetahui arus dan beda potensial serta nilai kerapatan daya yang dihasilkan dari proses metabolisme substrat molase menggunakan bakteri Pseudomonas sp. Penelitian ini menggunakan sistem MFC kompartemen ganda sebanyak dua sel yang dirangkai seri. Ruang anoda berisi campuran substrat molase, buffer kalium fosfat pH 7 dan bakteri Pseudomonas sp. Adapun ruang katoda berisi larutan elektrolit KMnO4 0,2 M. Pengukuran arus dan beda potensial dilakukan setiap 4 jam selama 36 jam. Berdasarkan hasil penelitian yang dilakukan didapatkan nilai arus, beda potensial maksimum dan kerapatan daya masing-masing sebesar 1656 mV, 1582 µA dan 1794,37 mW/m2.Kata kunci: dua sel, microbial fuel cell, molase, Pseudomonas sp
Enkapsulasi Asap Cair Tandan Kosong Kelapa Sawit (Elaeis guineensis Jacq.) dan Uji Aktivitas Antijamur Riskawati; Nurul Magfira; Maswati Baharuddin; Sappewali; Fitria Azis
Al-Kimia Vol 10 No 1 (2022): JUNI
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v10i1.25723

Abstract

Oil palm empty bunches is lignocellulose that can be processed into liquid smoke with the content of acid compounds and phenol compounds that function as a natural preservative. The purpose of this study to find out the characteristics of liquid smoke, see the effect of encapsulan on the liquid smoke encapsulation process, and see the antifungal activity of A. flavus and A. niger. The method used is pyrolysis process at a temperature of 400 oC to produce liquid smoke and encapsulation process by using variation of coating material using spray dryer with micro sized result. The result showed that the liquid smoke characteristics can be seen from its chemical content that is the dominant phenol compound and has the highest phenol content among the parts found in oil palm that is 68,15%. Based on analysis it can be seen that the best encapsulation process is by the addition of chitosan on maltodextrin with water content 5.78%, phenol content of 43.03 ppm and has a high rate of encapsulation efficiency of 45.87%. The liquid smoke microcapsules could inhibit the growth of fungi with the highest value of liquid smoke concentration, that is 3% with inhibitory value of 8.30 mm.
Production, Purification and Characterization of Cellulase from Cossus cossus Larvae Maswati Baharuddin
Al-Kimia Vol 10 No 1 (2022): JUNI
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v10i1.28071

Abstract

This study aimed to purify and characterize the biochemical properties of cellulase from bacterial isolates of C. cossus larvae, to determine the effect of adding crude extract cellulase and pure enzymes to the hydrolysis of rice straw. The research steps included purification of enzymes by ion exchange chromatography and gel filtration chromotography, as well as SDS-PAGE electrophoresis analysis. Pure cellulases are characterized by their biochemical properties and applied in the hydrolysis of cellulose. The results showed that the characteristics of an optimum pH of 5.6, an optimum temperature of 70⁰C and a substrate concentration of 1.5% CMC, and a molecular weight of 36.49 kDa.
Karakterisasi Enzim Amilase Isolat Bakteri R2M Larva Kumbang Sagu dari Luwu Utara Maswati Baharuddin; Nur Alfina; Amalyah Febryanti; Fitria Azis; Wulan Wahyuningsih
Chimica et Natura Acta Vol 10, No 2 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n2.37334

Abstract

Karakterisasi ekstrak kasar enzim amilase isolat bakteri R2M dari larva kumbang sagu telah dilakukan. Penelitian ini bertujuan untuk mengetahui aktivitas enzim amilase pada suhu, pH, dan konsentrasi substrat optimum terhadap substrat amilum murni dan amilum dari kulit singkong. Penentuan aktivitas enzim amilase menggunakan metode asam 3,5-dinitrosalisilat (DNS) yang diukur dengan spektrofotometer visible pada panjang gelombang (λ) 540 nm. Hasil penelitian menunjukkan bahwa enzim amilase isolat bakteri R2M larva kumbang sagu yang direaksikan dengan substrat amilum optimum pada suhu 70°C, pH 4, dan konsentrasi substrat 1,25% dengan aktivitas enzim masing-masing sebesar 0,116 U/mL; 0,049 U/mL; dan 0,044 U/mL. Aktivitas enzim amilase isolat bakteri R2M larva kumbang sagu yang direaksikan dengan substrat pati kulit singkong pada kondisi optimum (pH 4, suhu 70 °C, dan 1,25% amilum) sebesar 0,077 U/mL. Dengan demikian, enzim amilase isolate R2M yang diisolasi dari larva kumbang sagu mampu bekerja optimum pada konsentrasi substrat 1,25%; suhu 70°C, dan pH 4.
ISOLASI DAN IDENTIFIKASI KHAMIR PADA LARVA Cossus Cossus PENGHASIL SELULASE Maswati Baharuddin; Nurul Khaerah; Nurul Fadillah; Sappewali Sappewali; Fitria Azis
Teknosains Vol 16 No 3 (2022): September-Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i3.29381

Abstract

Larva Cossus cossus adalah serangga ordo Lepidoptera yang hidup di pohon menteng (Baccaurea racemosa) dari Desa Lejja Soppeng. Larva dapat menghasilkan selulase untuk menghidrolisis selulosa menjadi gula sederhana (glukosa) dengan memutuskan ikatan 1,4-glikosidik dalam selulosa. Selulase tekstil digunakan dalam industry detergen, makanan, dan kertas yang dapat diproduksi oleh mikroba seperti selulolitik ragi. Tujuan penelitian ini untuk mengetahui jenis isolat khamir dan bakteri serta aktivitas selulase simbion khamir larva C. cossus. Metode penelitian meliputi isolasi dan pemurnian khamir, identifikasi khamir dan uji aktivitas selulase yang dilakukan setengah kuantitatif (uji yodium dan uji Congo Red) serta uji kuantitatif dengan metode asam dinitrosalisilat (DNS) yang diukur dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri dan 3 isolat jamur yang bersimbiosis dengan larva C. cossus. Isolat dari bakteri Acinotobacter, Pseudomonas dan Bacillus. Isolat dari jamur diidentifikasi secara molekuler diidentifikasi isolat memiliki kesamaan DNA 94% sebagai Paphiopedilum concalor dan kesamaan 90% sebagai strain Cyberlindnera jadinii.
STUDY OF SECONDARY METABOLITES OF Jatropha gossypifolia LEAF EXTRACT AND ITS ACTIVITY AGAINST Propionibacterium acne Nunung Kurniati; Maswati Baharuddin; Ummi Zahra
Teknosains Vol 16 No 3 (2022): September-Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i3.29721

Abstract

Jarak merah merupakaan salah satu tanaman endemik Indonesia. Kandungan metabolit sekunder yang ada pada tanaman ini berpotensi untuk dijadikan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui potensi metabolit sekunder ekstrak jarak merah dalam menghambat pertumbuhan bakteri Propinium acne. Metode pengujian meliputi skrining fitokimia dan pengujian antibakteri dilakukan dengan metode sumuran. Hasil penelitian menunjukkan bahwa ekstrak daun jarak merah memiliki metabolit sekunder yaitu flavonoid, alkaloid dan steroid. Ekstrak etil asetat dan etanol daun jarak merah mampu menghambat pertumbuhan bakteri Propionibacterium acne dengan zona hambat berturut-turut sebesar 14,43 mm dan 9,37 mm.
Efektivitas ekstrak N-Heksana daun tembelekan (Lantana camara L.) dan mimba (Azadirachta indica A. Juss) terhadap mortalitas keong mas (Pomacea canaliculate L.) Mirna Mirna; Maswati Baharuddin; Ummi Zahra; Sappewali Sappewali
Jurnal AGRO Vol 10, No 1 (2023)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/24080

Abstract

Golden snail is one of the potential pests for agricultural failure in Indonesia. One of the methods used to control golden snail pests is the use of botanical pesticides. The purpose of this study was to determine the potential of L. Camara and A. Indica extracts as botanical pesticides to control the golden snail. The stages of research activities consisted of : extractionusing the maceration method; phytochemical screening; application of N-Hexane extract from tembelekan leaves and neem leaves; Observation of the golden snail mortality. The experimental method used was a completely randomized design (CRD) with nine treatments and three replications. Observations were made every 6 hours for 48 hours. Analysis of mortality data using ANOVA and DMRT follow-up test. The results showed that the secondary metabolite compounds from tembelekan and neem extracts that act as botanical pesticides are flavonoids, alkaloids, and triterpenoids. The most effective treatment in controlling the golden snail was the E3 treatment with a combination of 15% tembelekan n-hexane extract and 15% neem n-hexane extract achieving a 100% mortality percentage at 36 hours. Based on the analysis, it is known that the treatment of tembelekan and neem leaf extracts had a significant effect on golden snail mortality with Fcount (3.22) > Ftable (2.51).ABSTRAK Keong mas merupakan salah satu hama potensial terhadap kegagalan pertanian di Indonesia. Salah satu cara yang digunakan untuk mengendalikan hama keong mas yaitu menggunakan pestisida nabati. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak L. Camara dan A. Indica sebagai pestisida nabati untuk mengendalikan keong mas. Tahapan kegiatan penelitian terdiri dari: esktraksi dengan metode maserasi; skrining fitokimia; pengaplikasian ekstrak N-Heksana daun tembelekan dan daun mimba; pengamatan mortalitas keong mas. Metode eksperimen dengan rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan sembilan perlakuan dan tiga ulangan. Pengamatan dilakukan setiap 6 jam selama 48 jam. Analisis data mortalitas dengan menggunakan ANOVA dan uji lanjut DMRT. Hasil penelitian menunjukkan bahwa golongan senyawa metabolit sekunder dari ekstrak tembelekan dan mimba yang berperan sebagai pestisida nabati yaitu flavonoid, alkaloid, dan triterpenoid. Perlakuan yang paling efektif dalam mengendalikan keong mas yaitu pada perlakuan E3 dengan kombinasi ekstrak n-heksana tembelekan 15% dan ekstrak n-heksana mimba 15%. Berdasarkan analisisnya, diketahui bahwa perlakuan ekstrak daun tembelekan dan mimba tersebut memiliki pengaruh nyata terhadap mortalitas keong mas dengan Fhitung (3,22)>Ftabel (2,51).
Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati, Riskawati; Natsir, Hasnah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Isolation and Molecular Identification of Amylolytic Bacteria from Oryctes rhinoceros L. Larvae Decomposing Empty Palm Oil Fruit Bunches Uto, Sahriani; Arfah, Rugaiyah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6957

Abstract

Oryctes rhinoceros L. is an organism that helps the decomposition of oil palm empty fruit bunches (OPEFB). In the larvae's intestines, there are symbiotic bacteria that are used in the process of food degradation in the digestive system, one of which is amylolytic bacteria. This study aims to isolate and molecular identify amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The techniques are used to screen and isolate bacteria from horn beetle larvae. Bacterial identification was accomplished by microscopically identifying amylase-producing bacterial isolates, performing biochemical tests on selected bacterial isolates, quantifying amylase enzyme activity, and molecularly identifying 16S rRNA. The results of screening and bacterial isolation obtained five isolates. The largest amylolytic bacterial colony index value was obtained in the EA3 isolate, which was 1.370 mm. Bacterial isolates with the highest activity were found in isolates coded EA1 and EA2, namely 0.049 U/mL and 0.0479 U/mL. According to the findings of 16S rRNA molecular identification, isolates EA1 and EA2 had similarities with the bacteria Ochrobactrum sp. and Pseudomonas mendocina.
Chitinase Enzyme-Producing Endophytic Bacterias From the Roots of the Plant Gembolo (Dioscorea bulbifera): Isolation, Characterization and its Potential as an Antifungal Agent Yunita, Vivi Alfi; Natsir, Hasnah; Ahmad, Ahyar; Baharuddin, Maswati
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9422

Abstract

Chitinase is an enzyme of the chitinolytic group that has many roles in agriculture, especially as an antifungal, because chitin is one of the constituent components of the fungal cell wall. This study aimed to isolate and identify endophytic bacteria from gembolo (Dioscorea bulbifera) roots and to characterize the chitinase enzyme from these endophytic bacteria to be used as an antifungal. Isolation and identification of gembolo plant root endophytic bacteria using PCR method of 16S rRNA gene amplification and sequencing. Characterization of the chitinase enzyme produced includes determining of optimum pH, temperature, and substrate UV-Vis spectrophotometer. Antagonistic test of the chitinase enzyme and endophytic bacterial isolates (isolate K4) Fusarium oxysporum. The results showed that bacterial isolate K4 had with chitinolytic index of 2.45 mm. Electrophoresis results with PCR 16s rRNA gene; the length of the amplified fragment is the position of 1300 bp. By doing the BLAST process in GenBank, the bacterial isolate has 97.93% similarity with Enterobacter cloacae. Then, this endophytic bacteria is called Enterobacter cloacae K4-G. This bacterium produced chitinase enzyme reaching maximum chitinase activity at the 38 hours with an activity of 0.0312 U/mL. The chitinase characterization results of E. cloacae K4-G showed that the optimum conditions were reached at at pH 6, temperature 45 ᵒC, and 2.5% substrate with a chitinase activity value of 0.2467 U/mL. Chitinase enzyme and bacteria Enterobacter cloacae K4-G can inhibit the growth of the fungus Fusarium oxysporum. Therefore, Chitinase from Enterobacter cloacae K4-G can be used as an antifungal pathogen in plants.
Co-Authors Abd. Rauf Patong Abdul Karim Abeng, Tendri Ahyar Ahmad Ahyar Ahmad Aisyah Aisyah Al Maratun Sholihati Amalyah Febryanti Amalyah Febryanti Amalyah Febryanti Andi Nur Fitriani Abubakar Arfah, Rugaiyah Arfiani Nur Asriani Ilyas Febryanti, Amalyah Firnanelty, Firnanelty Fitria Azis Fitria Azis Fitriana Fitriana Fitriyani, Jeni Hasnah Natsir Heri Heriyono Ika Prestianti Ilmi Wahyuni Irma Rahayu Ismawanti Ismawanti Jeni Fitriyani Karisma Karisma Karisma, Karisma Kurniati, Nunung Mahmudah, Rafiah Maming Maming Mirna Mirna Muhammad Zakir Musyarrifah Musyarrifah Nunung Kurniati Nur Alfina Nur Asmi Nurdia Asdar Nursiah La Nafie Nurul Fadillah Nurul Khaerah Nurul Magfira Patunrengi, Iswadi Ibrahim Rina Dwismar Riskawati Riskawati Riskawati, Riskawati Rugaiyah Andi Arfah Rustiah, Wa Ode Santi Santi Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali, Sappewali Seniwati Dali Sitti Chadijah Sitti Chadijah Sjamsiah, Sjamsiah St Chadijah St. Chadijah Sufriyana Ali Syamsidar HS Ummi Zahra, Ummi Uto, Sahriani Wahyu Rizandi Wahyuti Wahyuti Wulan Wahyuningsih Yunita, Vivi Alfi Yusril Yusril