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All Journal AL KAUNIYAH Alchemy : Journal of Chemistry Teknosains: Media Informasi Sains dan Teknologi Jurnal Agro Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research Walisongo Journal of Chemistry Lontara Abdimas : Jurnal Pengabdian kepada Masyarakat Automotive Experiences Molekul: Jurnal Ilmiah Kimia

Maswati Baharuddin

Universitas Islam Negeri Alauddin Makassar

Author-ID : 239898

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Nursing Physics Public Health

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Produksi dan Uji Aktivitas Enzim Selulase dari Bakteri Bacillus subtilis Al Maratun Sholihati; Maswati Baharuddin; Santi Santi
Al-Kimia Vol 3 No 2 (2015): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Enzyme cellulose can be produced from bacteria Bacillus subtilis. This bacteria is a kind of bacteria which grounded on the genus that can degrade cellulose to glucose. Cellulase enzyme acquired different optimum pH and temperature depends on the bacteria. This research aim to produce and analyze the activity of the cellulose enzyme from bacteria Bacillus subtilis at optimum pH and temperature. The research of this enzyme cellulose derived by prolific process and rejuvenation bacteria Bacillus subtilis on the nutrition culture, nutrition selective, and nutrition production that executed by cold centrifugation to obtain extract or the enzyme cellulose at the temperature of 4°C, 3500 rpm within 15 minutes. On the determine pH with variety pH 5,6 using buffer acetat and pH 6,0; 6,5; 7,0; 7,5; and 8.0 using phosphate buffer while on the determine temperature using variety temperature 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C which the next make an experiment activity enzyme cellulose by method Nelson-Somogy that measured in spectrophotometer UV-Vis of λ 540 nm. The result shows that the highest enzyme activity at optimum pH of 6, 0 is 4,3661 10ˉ3 U/mL and in optimum temperature of 30°C as is 5,6609 10ˉ3 U/mL.

Isolasi dan Identifikasi Bakteri Pendegradasi Fenol yang Bersumber Dari Danau Tempe Kabupaten Wajo Sulawesi Selatan Fitriana Fitriana; Maswati Baharuddin; Sappewali Sappewali
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Water pollution in Tempe Lake was consequence by people activity in around of Tempe Lake, such as domestic waste and agriculture waste. One of the dangerous pollutions which was resulted from the waste was phenol pollution. Phenol waste reduction efforts on Tempe Lake could be solved by biodegradation process using bacteria. This research aimed to: 1) Get the bacteria which was able to degrade phenol on samples sourced from Tempe Lake, Wajo South Sulawesi and 2) Identify the type of bacteria that can degrade phenol sourced from Lake Tempe, Wajo South Sulawesi. The Metods used in the research was making of media, isolation and purification of bacteria, identification of bacteria and testing the ability of phenol degradation. Bacterial identification tests include staining gram and biochemical tests (TSIA, SIM, MR-VP, urea, citric and sugar fermentation). The degradation test was performed using UV-Vis spectrophotometry with colorimetric method using follin reagent. The results showed that the bacterial identified as the genus Enterobacter spp and Klebsella spp which were gram negative bacteria. Test of phenol degradation at 48 hour incubation using UV-Vis shows that bacterial isolates C1F was able to degrade phenol 500 ppm to 3,091 ppm, bacterial isolate S1F was able to degrade phenol 500 ppm to 5,1153 ppm and bacterial isolate H2F was able to degrade phenol 500 ppm to 7,7834 ppm.

Potensi Instrumen FTIR dan GC-MS dalam Mengkarakterisasi dan Membedakan Gelatin Lemak Ayam, Itik dan Babi St Chadijah; Maswati Baharuddin; Firnanelty Firnanelty
Al-Kimia Vol 7 No 2 (2019): DECEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Penelitian ini bertujuan mengkarakterisasi gelatin tulang kaki ayam, kulit itik dan kulit babi serta membedakan profil asam lemak dari ketiga material tersebut. Metode yang digunakan: proses curing dan hidrolisis dengan variasi suhu. Hasil yang diperoleh dikarakteisasi dengan FTIR dan GC-MS. Hasil yang diperoleh menunjukkan gelatin yang dihasilkan dari tulang kaki ayam, kulit babi dan kulit itik pada suhu 80ᵒC masing-masing 2.02%; 9.33% dan 1.1%. Kadar air dari tulang kaki ayam, kulit babi, dan kulit itik yaitu 11.19%; 7.73% dan 7.7%. kadar air tersebut telah memenuhi standar kadar air SNI yaitu maksimum 16%. Hasil karakterisasi gelatin dengan FTIR menunjukkan serapan gugus fungsi yang spesifik. Pada spektrum FTIR gelatin kulit babi terdapat gugus N−H dan O−H (3433,79 cm-1), CH2 (2931,01 cm-1), C═O (1655,21 cm-1), N−H dan C-N (1544,38 cm-1), N−H (1237,39 cm-1) dan gugus C−O (1079,69 cm-1). Peak yang dihasilkan kulit babi lebih sedikit. Sedangkan GC-MS mampu membedakan komponen asam lemak babi dengan asam lemak ayam dan itik. Diperoleh hasil bahwa komposisi asam lemak utama pada lemak babi adalah asam oleat C18:1 (58,79%), stearat C18:0 (11,66%) dan palmitat C16:0 (11,44%). Komponen asam lemak utama pada lemak babi murni secara keseluruhan memiliki asam arakidonat dan asam eikosenat yang tidak terdeteksi pada lemak lain.

ANALISIS PERBEDAAN KANDUNGAN LIPIDA MIKROALGA (Tetraselmis chuii dan Nannochloropsis oculata) PADA AIR LAUT DAN AIR PAYAU Maswati Baharuddin
Teknosains Vol 5 No 1 (2011): JANUARI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v5i1.164

This research quality eksperiment with different extracted of lipid microalgae Tetraselmis chuii dan Nannochloropsis oculata from two places from sea water and brachis water with pH and salinity to be different. Culture to do for eigh day just process extracted to do for ± three day. Extraction of lipid that had been done using chloroform : methanol (2:1) . product research can to be different growth and pregnancy womb content of lipid that can to do with use sea water media and brachis water media. The result of the research have found that % total lipid will obtained more when growing by using brackish water medias that is obtained counted 15,9 % for the Tetraselmis of chuii and 17,51% for the Nannochloropsis of oculata, while if using sea-water media only obtained 14,8 % and 16,3%. This matter is influenced by some factors like temperature, light intensity, and salinitas of pH.

PENGARUH SUHU DAN pH TERHADAP HIDROLISIS CMC OLEH ENZIM SELULASE DARI ISOLAT BAKTERI LARVA KUPU-KUPU COSSUS COSSUS Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Teknosains Vol 8 No 3 (2014): Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v8i3.1837

Cellulase enzymes can be isolated from microorganisms that are resistant to high pH and temperature. Cellulase enzyme has a different character depending on the source and the enzyme environment. This study aimed to characterize the cellulase enzymes from bacteria isolates CC2 dan CC4 which includes the determination of the optimum pH and temperature of the enzyme activity. In this study, an enzyme produced from isolates of bacteria larva Cossus cossus by centrifugation cold 4 ° C with a speed of 3000 rpm for 15 min to obtain a crude extract of the enzyme cellulase. Determination of pH performed using sitrat acid buffer(3; 3,6; 4; 4,6; 5;5, dan 5) and phosphate buffer with pH variation (6,0; 6,5; 7,0; 7,5; dan 8,0), while for the determination of done an optimum temperature variations in temperature (20, 30, 40, 50, 60, 70, 80, dan 90) ° C enzyme activity were further tested using the Nelson-Samogy measured on a UV-Vis spekrofotometer at a wavelength of 545 nm. Result showed greatest activity isolat CC2 at pH 7,5 enzyme cellulase activity of 15,8806 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 19,4121 x 10-4 U / mL. Greatest activity isolat CC4 at pH 4 enzyme cellulase activity of 15,4069 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 20,3487 x 10-4 U / mL

OPTIMALISASI LABORATORIUM RISET KIMIA FAKULTAS SAINSTEK UIN ALAUDDIN MAKASSAR DALAM MENINGKATKAN KUALITAS HASIL PENELITIAN MAHASISWA Sitti Chadijah; Maswati Baharuddin; Syamsidar HS
Teknosains Vol 9 No 1 (2015): JANUARI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v9i1.1855

Laboratorium riset merupakan salah satu laboratorium padajurusan kimia fakultas sains dan Tekhnologi. Laboratorium ini mempunyaifungsi sebagai laboratorium pendidikan dan Penelitian. Selama inipengembangan laboratorium riset telah dilakukan. Untuk mengetahuibagaimana optimalisasi laboratorium ini maka dilakukan penelitian dengantujuan (1) Untuk mengetahui optimalisasi kinerja laboratorium riset kimiadalam melayani mahasiswa yang melakukan penelitian, dan (2) Untukmengetahui kualitas hasil penelitian mahasiswa dengan menggunakaninstrumen di laboratorium riset kimia. Dalam melakukan penelitian inidilakukan pengukuran terhadap 3 variabel yaitu : personil, metode analisisdan peralatan. Indikator personal terbentuk dari tiga faktor atau konstrukyaitu: Mampu memberikan penjelasan dan informasi yang memadai (X11),Problem solvent (X12), Kompeten dalam bidangnya (X13). Sedangkanindikator metode analisis (X2) terbentuk dari beberapa konstruk, yaitu:Terdapat SOP dan Instruksi kerja (X21), Desain hasil sesuai Denganharapan (X22), memenuhi standar Keselamatan dan Kesehatan kerja (X23),Metode Kerja sesuai dengan standar ISO (X24).Peralatan (X3) terbentukdari beberapa konstruk, yaitu: Ketersediaan bahan / pereaksi (X31),Ketersediaan Peralatan (X32), Peralatan sesuai dengan spesifikasi(X33).Sedangkan untuk pengumpulan data kami menggunakan metodepengumpulan data dengan menggunakan observasi, wawancara dan angketatau kuisioner. Dari penelitian yang telah kami lakukan diperoleh (1)Optimalisasi laboratorium berdasarkan variabel personil, metode analisisdan peralatan dapat meningkatkan pelayanan terhadap mahasiswa yangmelakukan penelitian dan (2) Penggunaan laboratorium riset denganmengoptimalkan variabel personil, metode analisis dan peralatan dapatmeningkatkan kualitas hasil penelitian mahasiswa..

PRODUKSI BIOETANOL DARI JERAMI PADI (Oryza sativa L.) dan KULIT POHON DAO (Dracontamelon) MELALUI PROSES SAKARIFIKASI DAN FERMENTASI SERENTAK (SFS) Maswati Baharuddin; Sappewali Sappewali; Karisma Karisma; Jeni Fitriyani
Chimica et Natura Acta Vol 4, No 1 (2016)
Publisher : Departemen Kimia

Bioetanol merupakan energi alternatif yang dapat diproduksi dari biomassa seperti jerami padi dan biomassa berlignoselulosa. Biomassa lignoselulosa tersedia terus menerus dalam jumlah yang banyak, tetapi hanya dimanfaatkan sebagai pakan ternak, dan kadang-kadang sisanya dibakar. Jerami padi dan pohon dao memiliki komponen selulosa, hemiselulosa dan lignin. Penelitian ini bertujuan untuk mengetahui kadar glukosa yang dihasilkan melalui proses hidrolisis jerami padi dan pohon dao menggunakan enzim selulase serta menentukan kadar etanol yang dihasilkan dari proses fermentasi glukosa menggunakan Saccharomyces cerevisiae. Proses sakarifikasi dan fermentasi serentak (SFS) dilakukan dalam reaktor yang sama. Hasil penelitian menunjukkan bahwa fermentasi selama 3, 5, 7 dan 9 hari dihasilkan kadar dan konsentrasi bioetanol yang berbeda. Dari penelitian yang dilakukan kadar glukosa jerami padi adalah 105 mg/L dan dari kulit pohon dao sebanyak 216 mg/L.Volume bioetanol tertinggi pada hari ke-7 dengan tingkat kemurnian tertinggi pada pohon dao 0,97% pada jerami padi dengan 0,24%.

Peluang Usaha "Pembuatan Sabun Cuci Piring" Untuk Meningkatkan Pendapatan Keluarga Di Kelurahan Paropo Kec. Panakukang Kota Makassar Wa Ode Rustiah; St. Chadijah; Maswati Baharuddin; Sjamsiah Sjamsiah
Lontara Abdimas : Jurnal Pengabdian Kepada Masyarakat Vol 1 No 1 (2020): Juni
Publisher : Lembaga Penelitian Dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Abstract The problem that occurs in the Paropo sub-district, Panakkukang district, Makassar, is the large number of family heads with high dependence on the using of cleaning soap from dirt on an object. The results of a conducted survey, people generally switch to using liquid dishwashing soap, due to the many advantages of liquid soap, also practical because it is available in bottle form. However, limited funds, lack of knowledge about chemicals how to make liquid soap, and how to make it, until finally becoming an obstacle in opening up business opportunities going forward. The material was delivered through counseling using a combination of lecture, discussion and evaluation methods, and also continued to provide training with demonstrations on how to make liquid soap. From the community service activities, it provides enormous benefits to the community. It can be seen from the community's desire to make liquid soap independently for use among their families. Then start ordering raw materials to provide guidance in terms of good and durable packaging, so that this activity becomes a mover in marketing the results of the home industry.

Isolation of Cellulose-Degrading Bacteria from Luwu Timur in Oil Palm Empty Fruits Bunch Amalyah Febryanti; Maswati Baharuddin; Irma Rahayu
Walisongo Journal of Chemistry Vol 4, No 2 (2021): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v4i2.9173

The oil palm empty fruit bunch (OPEFB) is one of the solid wastes produced by industrial factories. These bunches are the habitat of cellulolytic bacteria. This study aims to identify the morphology and determine the potential microorganisms in degrading oil palm empty fruit bunches waste. The methods used in this study were isolation and observation of bacterial morphology, bacterial screening, degradation test of empty oil palm fruit bunches waste, and enzyme activity test with DNS method using UV-Vis spectrophotometer at a wavelength of 530 nm. A collection of 14 isolates were obtained from the isolation process. We obtained 14 isolates from the sample, the largest clear zone was shown by isolate S10. The isolate also exhibited the highest cellulose-degrading activity after 7 days incubation period with a degrading percentage of 13.27%. After incubation for 2 days, the morphological characteristics of the bacteria produced have circular and medium-sized colonies, yellow, colonies wave edges and flat colonies, elevation convex and smooth surface. The resulting enzyme activity was 0.1308 U/mL. Therefore, isolate S10 was suspected to have the potential to degrade plastic.

SKRINING MIKROBA PENDEGRADASI PLASTIK DARI TANAH DAN UJI BIODEGRADASI DENGAN FOURIER TRANSFORM INFRARED (FTIR) Nur Asmi; Maswati Baharuddin; Amalyah Febryanti
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.19826

AbstrakSaat ini pencemaran lingkungan telah menjadi permasalahan global, tidak hanya dirasakan oleh negara maju tetapi juga dialami oleh negara berkembang. Penyebab terjadinya pencemaran tersebut ialah adanya benda asing masuk ke dalam lingkungan, sehingga mengakibatkan kerusakan komposisi dari komposisi awalnya. Salah satu penyebab pencemaran lingkungan adalah kehadiran limbah plastik. Limbah tersebut merupakan limbah yang sangat sulit didegradasi. Salah satu solusi alternatif yang dilakukan dalam mendegradasi plastik adalah dengan menggunakan mikroorganisme. Penelitian ini bertujuan untuk melakukan skrining mikroorganisme dalam mendegradasi plastik. Jenis plastik yang digunakan sebagai sampel adalah High-Density Polyethylene (HDPE). Metode yang digunakan dalam penelitian ini adalah skrining mikroba dan uji biodegradasi dengan memanfaatkan Fourier Transform Infrared Spectroscopy (FTIR). Hasil yang diperoleh dalam studi ini adalah terdapat 17 jumlah isolat. Zona bening besar ditunjukkan oleh isolat A12P. Potensi biodegradasi terbesar selama 4 bulan dengan persentase 17,9104% ditunjukkan oleh isolat A12P. Hasil degradasi menunjukkan bahwa kehadiran gugus C=O mengindikasikan terjadinya reaksi oksidasi dan kinerja enzim oleh mikroba. Oleh karena itu, isolat A12P memiliki potensi untuk mendegradasi plastik.AbstractNowadays environmental pollution has become a global problem, which is not only experienced by developed countries but also by developing countries. The cause of this pollution is the presence of foreign objects entering the environment resulting in damage to the composition of the original composition. One of the causes of environmental pollution is the presence of plastic waste. This waste is very difficult to degrade. One of the alternative solutions to degrade plastics is to use microorganisms. This study aims to screen microorganisms to degrade plastics. The type of plastic used as the sample was High-Density Polyethylene (HDPE). The method used in this research was microbial growth screening and biodegradation testing using Fourier transform infrared spectroscopy (FTIR). The results obtained 17 isolates. The largest clear zone was shown by isolate A12P. The greatest biodegradation potential for 4 months with a percentage of 17.9104% was shown by isolate A12P. The results of degradation indicated that the presence of the C=O group caused the occurrence of oxidation reactions and enzyme performance by microbes. Therefore, A12P isolate has the potential to degrade plastics.

Co-Authors Abd. Rauf Patong Abdul Karim Abeng, Tendri Ahyar Ahmad Ahyar Ahmad Aisyah Aisyah Al Maratun Sholihati Amalyah Febryanti Amalyah Febryanti Amalyah Febryanti Andi Nur Fitriani Abubakar Arfah, Rugaiyah Arfiani Nur Asriani Ilyas Febryanti, Amalyah Firnanelty, Firnanelty Fitria Azis Fitria Azis Fitriana Fitriana Fitriyani, Jeni Hasnah Natsir Heri Heriyono Ika Prestianti Ilmi Wahyuni Irma Rahayu Ismawanti Ismawanti Jeni Fitriyani Karisma Karisma Karisma, Karisma Kurniati, Nunung Mahmudah, Rafiah Maming Maming Mirna Mirna Muhammad Zakir Musyarrifah Musyarrifah Nunung Kurniati Nur Alfina Nur Asmi Nurdia Asdar Nursiah La Nafie Nurul Fadillah Nurul Khaerah Nurul Magfira Patunrengi, Iswadi Ibrahim Rina Dwismar Riskawati Riskawati Riskawati, Riskawati Rugaiyah Andi Arfah Rustiah, Wa Ode Santi Santi Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali Sappewali, Sappewali Seniwati Dali Sitti Chadijah Sitti Chadijah Sjamsiah, Sjamsiah St Chadijah St. Chadijah Sufriyana Ali Syamsidar HS Ummi Zahra, Ummi Uto, Sahriani Wahyu Rizandi Wahyuti Wahyuti Wulan Wahyuningsih Yunita, Vivi Alfi Yusril Yusril

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