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All Journal Teknosains: Media Informasi Sains dan Teknologi Al-Kimia Chimica et Natura Acta Automotive Experiences Molekul: Jurnal Ilmiah Kimia
Maswati Baharuddin
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Environmental Science Materials Science & Nanotechnology Mechanical Engineering Physics

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Isolasi dan Identifikasi Bakteri Pendegradasi Fenol yang Bersumber Dari Danau Tempe Kabupaten Wajo Sulawesi Selatan Fitriana Fitriana; Maswati Baharuddin; Sappewali Sappewali
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (847.388 KB) | DOI: 10.24252/al-kimia.v4i2.1694

Abstract

Water pollution in Tempe Lake was consequence by people activity in around of  Tempe Lake, such as domestic waste and agriculture waste. One of the dangerous pollutions which was resulted from the waste was phenol pollution. Phenol waste reduction efforts on Tempe Lake could be solved by biodegradation process using bacteria. This research aimed to: 1) Get the bacteria which was able to degrade phenol on samples sourced from Tempe Lake, Wajo South Sulawesi and 2) Identify the type of bacteria that can degrade phenol sourced from Lake Tempe, Wajo South Sulawesi. The Metods used in the research was making of media, isolation and purification of bacteria, identification of bacteria and testing the ability of phenol degradation. Bacterial identification tests include staining gram and biochemical tests (TSIA, SIM, MR-VP, urea, citric and sugar fermentation). The degradation test was performed using UV-Vis spectrophotometry with colorimetric method using follin reagent. The results showed that the bacterial identified as the genus Enterobacter spp and Klebsella spp which were gram negative bacteria. Test  of phenol degradation at 48 hour incubation using UV-Vis shows that bacterial isolates C1F was able to degrade phenol 500 ppm to 3,091 ppm, bacterial isolate S1F was able to degrade phenol 500 ppm to 5,1153 ppm and bacterial isolate H2F was able to degrade phenol 500 ppm to 7,7834 ppm.
Potensi Instrumen FTIR dan GC-MS dalam Mengkarakterisasi dan Membedakan Gelatin Lemak Ayam, Itik dan Babi St Chadijah; Maswati Baharuddin; Firnanelty Firnanelty
Al-Kimia Vol 7 No 2 (2019): DECEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.727 KB) | DOI: 10.24252/al-kimia.v7i2.7521

Abstract

Penelitian ini bertujuan mengkarakterisasi gelatin tulang kaki ayam, kulit itik dan kulit babi serta membedakan profil asam lemak dari ketiga material tersebut. Metode yang digunakan: proses curing dan hidrolisis dengan variasi suhu. Hasil yang diperoleh dikarakteisasi dengan FTIR dan GC-MS. Hasil yang diperoleh menunjukkan gelatin yang dihasilkan dari tulang kaki ayam, kulit babi dan kulit itik pada suhu 80ᵒC masing-masing 2.02%; 9.33% dan 1.1%. Kadar air dari tulang kaki ayam, kulit babi, dan kulit itik yaitu 11.19%; 7.73% dan 7.7%. kadar air tersebut telah memenuhi standar kadar air SNI yaitu maksimum 16%. Hasil karakterisasi gelatin dengan FTIR  menunjukkan serapan gugus fungsi yang spesifik. Pada spektrum FTIR gelatin kulit babi terdapat gugus N−H dan O−H (3433,79 cm-1), CH2 (2931,01 cm-1), C═O (1655,21 cm-1), N−H dan C-N (1544,38 cm-1), N−H (1237,39 cm-1) dan gugus C−O (1079,69 cm-1). Peak yang dihasilkan kulit babi lebih sedikit. Sedangkan  GC-MS mampu membedakan komponen asam lemak babi dengan asam lemak ayam dan itik. Diperoleh hasil bahwa komposisi asam lemak utama pada lemak babi adalah asam oleat C18:1 (58,79%), stearat C18:0 (11,66%) dan palmitat C16:0 (11,44%). Komponen asam lemak utama pada lemak babi murni secara keseluruhan memiliki asam arakidonat dan asam eikosenat yang tidak terdeteksi pada lemak lain.
ANALISIS PERBEDAAN KANDUNGAN LIPIDA MIKROALGA (Tetraselmis chuii dan Nannochloropsis oculata) PADA AIR LAUT DAN AIR PAYAU Maswati Baharuddin
Teknosains Vol 5 No 1 (2011): JANUARI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v5i1.164

Abstract

This research quality eksperiment with different extracted of lipid microalgae Tetraselmis chuii dan Nannochloropsis oculata from two places from sea water and  brachis water  with  pH and salinity to be different. Culture to do for eigh day just process extracted to do for ± three day. Extraction of lipid that had been done using chloroform : methanol (2:1) . product research can to be different  growth and pregnancy womb content of lipid  that can to do with use sea water media and brachis water media. The result of the research have found that % total lipid will obtained more when growing by using brackish water medias that is obtained counted 15,9 % for the Tetraselmis of chuii and 17,51% for the Nannochloropsis of oculata, while if using sea-water media only obtained 14,8 % and 16,3%. This matter is influenced by some factors like temperature, light intensity, and salinitas of pH.
PENGARUH SUHU DAN pH TERHADAP HIDROLISIS CMC OLEH ENZIM SELULASE DARI ISOLAT BAKTERI LARVA KUPU-KUPU COSSUS COSSUS Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Teknosains Vol 8 No 3 (2014): Desember
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v8i3.1837

Abstract

Cellulase enzymes can be isolated from microorganisms that are resistant to high pH and temperature. Cellulase enzyme has a different character depending on the source and the enzyme environment. This study aimed to characterize the cellulase enzymes from bacteria isolates CC2 dan CC4 which includes the determination of the optimum pH and temperature of the enzyme activity. In this study, an enzyme produced from isolates of bacteria larva Cossus cossus by centrifugation cold 4 ° C with a speed of 3000 rpm for 15 min to obtain a crude extract of the enzyme cellulase. Determination of pH performed using sitrat acid buffer(3; 3,6; 4; 4,6; 5;5, dan 5) and phosphate buffer with pH variation (6,0; 6,5; 7,0; 7,5; dan 8,0), while for the determination of done an optimum temperature variations in temperature (20, 30, 40, 50, 60, 70, 80, dan 90) ° C enzyme activity were further tested using the Nelson-Samogy measured on a UV-Vis spekrofotometer at a wavelength of 545 nm. Result showed greatest activity isolat CC2 at pH 7,5 enzyme cellulase activity of 15,8806 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 19,4121 x 10-4 U / mL. Greatest activity isolat CC4 at pH 4 enzyme cellulase activity of 15,4069 x 10-4 U / mL while the optimum temperature of 70 ° C with the activity obtained at 20,3487 x 10-4 U / mL
OPTIMALISASI LABORATORIUM RISET KIMIA FAKULTAS SAINSTEK UIN ALAUDDIN MAKASSAR DALAM MENINGKATKAN KUALITAS HASIL PENELITIAN MAHASISWA Sitti Chadijah; Maswati Baharuddin; Syamsidar HS
Teknosains Vol 9 No 1 (2015): JANUARI
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v9i1.1855

Abstract

Laboratorium riset merupakan salah satu laboratorium padajurusan kimia fakultas sains dan Tekhnologi. Laboratorium ini mempunyaifungsi sebagai laboratorium pendidikan dan Penelitian. Selama inipengembangan laboratorium riset telah dilakukan. Untuk mengetahuibagaimana optimalisasi laboratorium ini maka dilakukan penelitian dengantujuan (1) Untuk mengetahui optimalisasi kinerja laboratorium riset kimiadalam melayani mahasiswa yang melakukan penelitian, dan (2) Untukmengetahui kualitas hasil penelitian mahasiswa dengan menggunakaninstrumen di laboratorium riset kimia. Dalam melakukan penelitian inidilakukan pengukuran terhadap 3 variabel yaitu : personil, metode analisisdan peralatan. Indikator personal terbentuk dari tiga faktor atau konstrukyaitu: Mampu memberikan penjelasan dan informasi yang memadai (X11),Problem solvent (X12), Kompeten dalam bidangnya (X13). Sedangkanindikator metode analisis (X2) terbentuk dari beberapa konstruk, yaitu:Terdapat SOP dan Instruksi kerja (X21), Desain hasil sesuai Denganharapan (X22), memenuhi standar Keselamatan dan Kesehatan kerja (X23),Metode Kerja sesuai dengan standar ISO (X24).Peralatan (X3) terbentukdari beberapa konstruk, yaitu: Ketersediaan bahan / pereaksi (X31),Ketersediaan Peralatan (X32), Peralatan sesuai dengan spesifikasi(X33).Sedangkan untuk pengumpulan data kami menggunakan metodepengumpulan data dengan menggunakan observasi, wawancara dan angketatau kuisioner. Dari penelitian yang telah kami lakukan diperoleh (1)Optimalisasi laboratorium berdasarkan variabel personil, metode analisisdan peralatan dapat meningkatkan pelayanan terhadap mahasiswa yangmelakukan penelitian dan (2) Penggunaan laboratorium riset denganmengoptimalkan variabel personil, metode analisis dan peralatan dapatmeningkatkan kualitas hasil penelitian mahasiswa..
Isolation and Identification of Cellulolytic Bacteria from Gut of Horn Beetle Larvae (Oryctes rhinoceros L.) Riskawati, Riskawati; Natsir, Hasnah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6848

Abstract

The horn beetle larvae (Oryctes rhinoceros L.) contain symbiotic bacteria that are used to digest and degrade cellulose as food so that it has the potential to produce cellulase enzymes. This study aims to isolate, characterize and identify microbial symbionts from horn beetle larvae that have the potential to produce cellulase enzymes.The methods in this study include morphology and physiology identification of bacteria, qualitative and quantitative activity tests and species determination using 16S rRNA sequencing technique. Based on the results of morphological observations, five bacterial isolates were taken which has the potential as a cellulase producer is indicated by the presence of a clear zone that is produced when a qualitative test is carried out using congo red staining with different cellulolytic indices. Based on the quantitative bacterial activity test using UV-Vis, the highest activity was found in PES3 isolates at 1.62 x 10-2 and PES5 at 1.61 x 10-2. Species determination results found that PES3 isolates belonged to the genus Acinetobacter and PES5 belonged to Pseudomonas. In addition to the isolates obtained for the environment and the industrial sector, cellulolytic bacteria can provide added value such as hydrolyze cellulose waste into alternative fuels.
Isolation and Molecular Identification of Amylolytic Bacteria from Oryctes rhinoceros L. Larvae Decomposing Empty Palm Oil Fruit Bunches Uto, Sahriani; Arfah, Rugaiyah; Dali, Seniwati; Baharuddin, Maswati
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.6957

Abstract

Oryctes rhinoceros L. is an organism that helps the decomposition of oil palm empty fruit bunches (OPEFB). In the larvae's intestines, there are symbiotic bacteria that are used in the process of food degradation in the digestive system, one of which is amylolytic bacteria. This study aims to isolate and molecular identify amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The techniques are used to screen and isolate bacteria from horn beetle larvae. Bacterial identification was accomplished by microscopically identifying amylase-producing bacterial isolates, performing biochemical tests on selected bacterial isolates, quantifying amylase enzyme activity, and molecularly identifying 16S rRNA. The results of screening and bacterial isolation obtained five isolates. The largest amylolytic bacterial colony index value was obtained in the EA3 isolate, which was 1.370 mm. Bacterial isolates with the highest activity were found in isolates coded EA1 and EA2, namely 0.049 U/mL and 0.0479 U/mL. According to the findings of 16S rRNA molecular identification, isolates EA1 and EA2 had similarities with the bacteria Ochrobactrum sp. and Pseudomonas mendocina.
Chitinase Enzyme-Producing Endophytic Bacterias From the Roots of the Plant Gembolo (Dioscorea bulbifera): Isolation, Characterization and its Potential as an Antifungal Agent Yunita, Vivi Alfi; Natsir, Hasnah; Ahmad, Ahyar; Baharuddin, Maswati
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.9422

Abstract

Chitinase is an enzyme of the chitinolytic group that has many roles in agriculture, especially as an antifungal, because chitin is one of the constituent components of the fungal cell wall. This study aimed to isolate and identify endophytic bacteria from gembolo (Dioscorea bulbifera) roots and to characterize the chitinase enzyme from these endophytic bacteria to be used as an antifungal. Isolation and identification of gembolo plant root endophytic bacteria using PCR method of 16S rRNA gene amplification and sequencing. Characterization of the chitinase enzyme produced includes determining of optimum pH, temperature, and substrate UV-Vis spectrophotometer. Antagonistic test of the chitinase enzyme and endophytic bacterial isolates (isolate K4) Fusarium oxysporum. The results showed that bacterial isolate K4 had with chitinolytic index of 2.45 mm. Electrophoresis results with PCR 16s rRNA gene; the length of the amplified fragment is the position of 1300 bp. By doing the BLAST process in GenBank, the bacterial isolate has 97.93% similarity with Enterobacter cloacae. Then, this endophytic bacteria is called Enterobacter cloacae K4-G. This bacterium produced chitinase enzyme reaching maximum chitinase activity at the 38 hours with an activity of 0.0312 U/mL. The chitinase characterization results of E. cloacae K4-G showed that the optimum conditions were reached at at pH 6, temperature 45 ᵒC, and 2.5% substrate with a chitinase activity value of 0.2467 U/mL. Chitinase enzyme and bacteria Enterobacter cloacae K4-G can inhibit the growth of the fungus Fusarium oxysporum. Therefore, Chitinase from Enterobacter cloacae K4-G can be used as an antifungal pathogen in plants.
Antibacterial and Antioxidant Activities of Cutibacterium acnes on Jatropha gossypifolia leaves and Pommetia pinata Bark Zahra, Ummi; Nur, Arfiani; Kurniati, Nunung; Yusril, Yusril; Baharuddin, Maswati
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.54007

Abstract

Cutibacterium acnes is a bacterium that can cause inflammation of the skin tissue and lead to acne. Inhibitory activity treatment can be carried out using natural compounds unique to Indonesia as a tropical country, namely Pometia pinnata stem bark and red Jatropha gossypifolia leaves. Pometia pinnata stem bark and red Jatropha gossypifolia leaves are known to have the potential for antibacterial activity. This study aims to combine the two plants as anti-bacterial agents against Cutibacterium acnes and as antioxidants. The method used in this study was maceration, employing ethanol and ethyl acetate solvents for antibacterial and antioxidant activity tests, specifically paper disc diffusion and DPPH assays. The results showed that J. gossypifolia and Pometia pinnata leaf extracts contain flavonoids, steroids, terpenoids, alkaloids, and tannins. The antibacterial activity of a mixture of ethanol extracts from J. gossypifolia and Pometia pinnata leaves against Cutibacterium acnes bacteria was strong, categorized at a concentration of 25%. Additionally, there was no significant difference in the antioxidant capacity between the mixture of ethanol and ethyl acetate extracts. The antioxidant capacity value was approximately 53% AEAC at a concentration of 500 ppm.
Isolation and Characterization of Cellulase Enzyme from Sago Bettle Larvae Febryanti, Amalyah; Baharuddin, Maswati; Abeng, Tendri
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.48974

Abstract

Sago beetle larvae are larvae that consume cellulose and convert it into simple compounds with the help of cellulase enzymes. The enzyme is produced by bacteria found in the larvae's intestines. This study aims to characterize the cellulase enzyme from R8W bacteria, which is a cellulolytic bacterium and derived from sago beetle larvae. The characterization in this research included determination of the optimum temperature, the optimum pH, and the optimum substrate concentration of the enzyme. The methods in this study consisted of the production of enzymes; characterization of cellulase enzyme by DNS method; and measurement of cellulase enzyme activity on natural substrates. The results showed that the cellulase enzyme R8W bacterial isolates from beetle larvae were in optimum conditions respectively at a temperature of 50 °C (enzyme activity of 0.070 U/mL), pH 8 (enzyme activity of 0.069 U/mL), substrate concentration of 2% (enzyme activity of 0.063 U/mL); cellulase enzyme activity of R8W bacterial isolates from sago beetle larvae on rice husk cellulose as a natural substrate was 0.103 U/mL. The characteristics of the cellulase enzyme of R8W bacterial isolate from sago beetle larvae had an optimum temperature of 50 °C, an optimum pH of 8, and an optimum substrate concentration of 2%.
Co-Authors Abd. Rauf Patong Abdul Karim Abeng, Tendri Ahyar Ahmad Ahyar Ahmad Aisyah Aisyah Al Maratun Sholihati Andi Nur Fitriani Abubakar Arfah, Rugaiyah Arfah, Rugaiyah Andi Arfiani Nur Asriani Ilyas Febryanti, Amalyah Firnanelty, Firnanelty Fitriana Fitriana Fitriyani, Jeni Hasnah Natsir Ismawanti Ismawanti Karisma, Karisma Kurniati, Nunung Maming Maming Muhammad Zakir Musyarrifah Musyarrifah Nurdia Asdar Nursiah La Nafie Rina Dwismar Riskawati Riskawati, Riskawati Santi Santi Sappewali Sappewali Sappewali, Sappewali Seniwati Dali Sitti Chadijah St Chadijah Sufriyana Ali Syamsidar HS Ummi Zahra, Ummi Uto, Sahriani Wahyu Rizandi Wahyuti, Wahyuti Yunita, Vivi Alfi Yusril Yusril