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WIDOWATI BUDIJASTUTI
Department Of Biology Faculty Of Mathematics And Natural Science Universitas Negeri Surabaya
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The Vulnerable Fishing Cat Prionailurus viverrinus from Wonorejo Mangroves, Surabaya, Indonesia Based on Morphology and Molecular Data Kuntjoro, Sunu; Rahayu, Dwi Anggorowati; Budijastuti, Widowati; Winarsih, Winarsih
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.29425

Abstract

The fishing cat (Prionailurus viverrinus) is a protected animal. Currently, the population in nature is declining due to over-fishing and changes in environmental quality. The existence of this fishing cat is an interesting finding that must be immediately studied at the morphological and genetic levels for certainty of its species. This study aimed to identify fishing cat from Wonorejo Mangroves, based on morphology and Cyt b genes analysis. The results of Cyt b identification obtained a DNA sequence length of 398 bp with a similarity value of the five Prionailurus sp. samples between 96.75 to 98.97%. The identification using molecular data consisted of five variations of nucleotide bases, and the average value of the genetic distance with the ingroup was 1.0%., The Cyt b DNA markers analysis succesfully identified fishing cat from Wonorejo Mangroves as Prionailurus viverrinus. 
Profil Miskonsepsi Peserta Didik Kelas XI Menggunakan Four-Tier Diagnostic Test dan Remediasi pada Submateri Transpor Membran Hidayati, Nurul; Budijastuti, Widowati
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 13 No. 2 (2024)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v13n2.p465-472

Abstract

Abstrak Miskonsepsi menjadi faktor terhadap penerimaan konsep pada peserta didik. Miskonsepsi perlu diidentifikasi dan diremediasi untuk mencegah kesalahpahaman terhadap konsep selanjutnya. Miskonsepsi adalah keyakinan konseptual peserta didik namun tidak sejalan dengan kebenaran di kalangan ahli. Penelitian ini bertujuan untuk menganalisis profil konsepsi peserta didik melalui instrumen four-tier diagnostic test dan mendeskripsikan hubungan antara remediasi Concept Attainment Model (CAM) dengan profil konsepsi peserta didik. Penelitian ini merupakan penelitian kuantitatif disertai survei dengan rancangan one group pretest-posttest design yaitu adaptasi soal four-tier diagnostic test, uji soal pretest, perlakuan CAM, uji soal posttest, analisis hasil penelitian dengan tabel kategori dan kriteria tingkat miskonsepsi, membandingkan hasil pretest dan posttest, dan wawancara. Subjek dalam penelitian ini adalah peserta didik XI-B Madrasah Aliyah Negeri 1 Pasuruan yang berjumlah 35 orang. Hasil penelitian menunjukkan bahwa rata-rata profil konsepsi siswa sebelum perlakuan yaitu miskonsepsi 15%, tidak paham konsep 8%, paham sebagian 49%, dan paham konsep 28%. Setelah perlakuan Concept Attainment Model (CAM), rata-rata profil konsepsi peserta didik miskonsepsi menurun menjadi 7%, tidak paham konsep menurun menjadi 2%, paham sebagian menurun menjadi 24%, dan paham konsep meningkat menjadi 67%. Hal ini membuktikan bahwa terdapat pengaruh atau perubahan sebelum dan sesudah diberikan remediasi pembelajaran Concept Attainment Model (CAM). Kata kunci: miskonsepsi, transpor membran, remediasi, Concept Attainment Model (CAM). Abstract Misconceptions are a factor in students' acceptance of concepts. Misconceptions need to be identified and remediated to prevent further misunderstanding of the concept. Misconceptions are students' conceptual beliefs that are not in line with the truth among experts. This research aims to analyze students' conceptual profiles using a four-tier diagnostic test instrument and describe the relationship between Concept Attainment Model (CAM) remediation and students' conceptual profiles. This research is quantitative research accompanied by a survey with a one-group pretest-posttest design, namely adaptation of four-tier diagnostic test questions, pretest question test, CAM treatment, posttest question test, analysis of research results using category tables and criteria for misconception levels, comparing pretest results and posttest, and interview. The subjects in this research were 35 students of XI-B Madrasah Aliyah Negeri 1 Pasuruan. The results of the research showed that the average profile of students' conceptions before treatment was 15% misconception, 8% did not understand the concept, 49% partially understood, and 28% understood the concept. After the Concept Attainment Model (CAM) treatment, the average conceptual profile of students with misconceptions decreased to 7%, not understanding the concept decreased to 2%, partial understanding decreased to 24%, and understanding the concept increased to 67%. This proves that there is an influence or change before and after being given Concept Attainment Model (CAM) learning remediation. Keywords: misconceptions, membrane transport, remediation, Concept Attainment Model (CAM).
FURTHER STUDY ON TWO SPECIES OF LOACH FISHES (Cypriniformes: Nemacheilidae: Nemacheilus) BASED ON MORPHOLOGY AND MOLECULAR DATA Rahayu, Dwi Anggorowati; Kuntjoro, Sunu; Budijastuti, Widowati; Winarsih, Winarsih; Ambarwati, Reni; Nugroho, Endik Deni; Basith, Abdul; Kurniawan, Nia; Haryono, Haryono
BIOTROPIA Vol. 30 No. 3 (2023): BIOTROPIA Vol. 30 No. 3 December 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1942

Abstract

The identities of two local loaches, Nemacheilus chrysolaimos (Valenciennes, 1846) and N. fasciatus (Valenciennes, 1846) from six rivers, were obtained through a comprehensive examination of their morphology and molecular characteristics in Biltar Regency, East Java, Indonesia. Therefore, this study identified Nemacheilus spp. from Blitar based on morphology and partial sequence of COI. The meristic data obtained for N. chrysolaimos included DII. 7–8 (dorsal fin), AI. 3–5 (anal fin), PI. 9 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). On the other hand, N. fasciatus exhibited the following meristic data, namely D II 7–8 (dorsal fin), AI. 6 (anal fin), PI. 9–10 (pectoral fin), VI. 6–7 (ventral fin), and C. 17 (caudal fin). A significant difference was observed in the morphometric characteristics of N. fasciatus across various sampling sites, as determined by the Kruskal-Wallis Test. Furthermore, the nucleotide base composition sequences of Nemacheilus spp. consisted of Thiamine (T), Cytosine (C), Adenine (A), and Guanine (G) with a mean of 29.565%, 32.023%, 23.88%, and 16.244%. Maximum Likelihood (ML) and Minimum Evolution (ME) phylogenetic analysis was also conducted using the Kimura 2 Parameter model to establish two major clades on Nemacheilus spp. and one out-group significantly different from the Nemacheilus spp. The results showed that these major clades exhibited a close relationship at 100% bootstrap support and were grouped under the genus Nemacheilus. The study on Nemacheilus spp. from the Blitar locality differentiated COI sequences between N. fasciatus and N. chrysolaimos. Additionally, N. chrysolaimos, as inferred from reference sequences, was identified as the ancestral species to N. chrysolaimos MZB 26540 and MZB 26539. ABGD analyses, employing a prior maximal distance of 0.025, also indicated the separation of these species into distinct partitions. The integration of morphology and genetic data for Nemacheilus spp. should provide valuable insights for future genetic population studies and conservation initiatives.
Co-Authors Abdul Basith Afannudin, Mas Agustina Fajar Rini Aisyah, Ramadhani Nur Akbarirrahman, Siti Nur Amritha, Malna Levy Aris Dwi Winanda Azizah, Vania Nur Dwi Anggorowati Rahayu Endik Deni Nugroho Faizah, Ulfi Fida Rahmadiarti Firda Dwi Novitasari Flaviana Claudia Andayani Haryono Haryono Indang Mustiko Rini Khaleyla, Firas Khayra Ummatin Nawfa Marinta, Madha Martika, Shinta Dwi Munawaroh, Nur Azizatul Muslih, Lindawati MUSLIMIN IBRAHIM Nia Kurniawan Ningrum, Tutut Novitasari, Firda Dwi Nugroho, Trias Nurfitrianto, Hendrik Nurul Hidayati Nuzulah, Dewi Firdausi Pramesti, Hervanny Putri Purnama, Erlix Rakhmad Raharjo, Raharjo Rahmadiarti, Fida Rani, Mustika Reni Ambarwati Rian Ningsih Pramunita Rudiana Agustini Sabila, Sefrinda Byartika Sari, Debby Verama Sifak Indana Sisilia Anita Adan Sunu Kuntjoro Suryanti Suryanti Sutiyanti, Ekris TARZAN PURNOMO Tri Wahyuning Utami Utami, Tri Wahyuning Vania Nur Azizah Wafiroh, Zaimatul Wahono Widodo Wihdatul Istiqah Winanda, Aris Dwi Winarsih Winarsih Zahrin Nur Azizah Zaimatul Wafiroh ZAINUL ARIFIN IMAM SUPARDI