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All Journal Jurnal Fitopatologi Indonesia IPTEKMA SOCA: Jurnal Sosial Ekonomi Pertanian AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan Cropsaver : Journal of Plant Protection
I G. R. M. TEMAJA
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Medicine & Pharmacology

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KERAGAMAN MIKOFLORA TANAH PADA HABITAT TANAMAN PISANG DI BALI l MADE SUDARMA; D.N. SUPRAPTA; Rai Maya Temaja
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 6 No 1 (2011)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Fungi in the soil plays an important role in maintaining the health and quality ofland, one of several indicators of soil health that is the diversity of soil fungi. This study was done in order to know the soil fungi diversity in the soil of banana plants habitat. The soil samples were collected from three regencies in Bali, i.e. Karangasem, Klungkung and Jembrana which are the main banana growing areas in Bali. Soil sampling was done in two sites in each regency, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm, with three replication. Soil microbes population density particularly for bacteria, fungi and actinomycetes were determined based on plate account technique, while the microbes diversity was determined based on Diversity Index of Shannon-Wiener. Diversity index of soil fungi of all soil samples ranged from 0.8785 to 2.1458 (criteria oflow to moderate), with population densities ranging from 1.1 x 104 to 2.8 x 104 cfu / g soil. Evenness index at all sites soil samples obtained ranged from 0.6688 to 0.9766, this means the fungus species found there are no outstanding domination. Similarity index on all soil samples showed less than 0.5, which means one does not have a kinship with each other. Physicochemical factors that affect population density of soil fungi on the banana plant habitats: organic C, total N, available P, available K, soil moisture content (air dry capacity and field capacity), sand and clay. W hile soil physicochemical factors that influence the number of species (diversity) of soil fungi namely: C-organic content, total N and the dust has positive influence on the number of species in banana plants habitat, whereas soil sand content negatively affected the number of types of soil fungi.
Uji Efektivitas Fungisida Alami dan Sintetis dalam Mengendalikan Penyakit Layu Fusarium pada Tanaman Tomat yang Disebabkan oleh Fusarium oxysporum f.sp. lycopersici LASTRI APRIANI; DEWA NGURAH SUPRAPTA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT The Test of Natural and Synthetic Fungicides Effectiveness in Controlling Fusarium Wilt of Tomato Plants Caused by Fusarium oxysporum f.sp. lycopersici Fusarium wilt caused by Fusarium oxysporum f.sp. lycopersici is one of important diseases in tomato plants. The emersion of the disease can result in huge loss for farmers. The use of synthetic fungicides that has been widely utilized all this time by farmers to control diseases caused by Fusarium oxysporum f.sp. lycopersici has adverse environmental impacts, therefore it is required biological fungicide that is more environmentally friendly. This study is purposed to testing the effectiveness of some natural fungicides and synthetic fungicide, is like a betel leaf, Trichoderma harzianum fungus, Klebsiella pneumoniae, and benomyl for suppress F. oxysporum f.sp. lycopersici and restrained Fusarium wilt diseases . The result of this study shows that the biological fungicide of betel leaf extract can inhibit the pathogenic F. oxysporum f.sp. lycopersici by 76.11 % and the percentage of inhibition potency of other benomyl fungicides reaches 61.11 % , while Klebsiella pneumoniae is by 54.42 % , and 20.98 % for Trichoderma harzianum on PDA medium. The result of field test shows that the extract of betel leaf can suppress Fusarium wilt by 5 % , T. harzianum , benomyl and K. pneumoniae are able to suppress Fusarium wilt respectively by 2.5 %. The use of T. harzianum and K. pneumoniae as biological control has the same effectiveness with synthetic fungicide in order to suppress Fusarium wilt.   Keywords: fusarium wilt, fusarium oxysporum f.sp. lycopersici, biological fungicide
Infeksi Virus Mosaik pada Tanaman Kedelai di Bali I WAYAN WIKA CENDANA PUTRA; I MADE SUDANA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Mosaic Virus Infections in Soybean Plants in Bali This study aimed to detect Soybean Mosaic Virus (SMV), Cucumber Mosaic Virus (CMV), and Potyvirus that infect soybean plants in Bali. The study was conducted from January to March 2017 located in nine observation locations of soybean crops throughout Bali. Observations were made on the symptoms found in the field and the incidence of the disease was determined by the Elisa (Enzyme Linked Immunosorbent Assay) serology test by using SMV, CMV, and Potyvirus antibodies.Field observations showed that the percentage of disease incidence with mosaic symptoms was 50%. Serologic assay results showed that in all samples tested were infected by CMV, SMV and Potyvirus and multiple infections between CMV-SMV, CMV-Potyvirus, and Potyvirus-SMV. Serological test results from the mosaic symptomatic samples showed the highest CMV infection occurred in Klungkung (60%), and the lowest were in Buleleng, Jembrana, Tabanan and Bangli respectively (30%). The highest SMV infection occurred in Denpasar (60%) and the lowest occurred in Karangasem (30%). The highest Potyvirus infection occurred in Denpasar (30%) and the lowest occurred or did not occur Potyvirus infection in Jembrana and Bangli (0.0%). Through serological tests, soybean plant samples from nine observation sites of soybean plants throughout Bali were positive against the antisera of SMV, CMV, and Potyvirus.
The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali NI LUH PUTU CITRA INNOSENSIA; I GEDE RAI MAYA TEMAJA; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The percentage of Bean Common Mosaic Virus (BCMV) carried by seeds and detection of virus position inside long beans (Vigna sinensis L.) seeds in Bali Long bean (Vigna sinensis L.) is a horticulture crop with an essential economic value in Indonesia and Bali. Long beans productivity in Bali reached 6023 tons in 2013, and decreased to 5157 tons in 2014. Cases of disease that lowers the production of long beans in Bali were caused by Bean Common Mosaic Virus (BCMV) infection by 56.06%. BCMV can be brought by the seeds (seed-borne disease) and can infect long beans plants since the early stage of development. This research aims to evaluate the percentage of BCMV transmission by commercial long beans seeds, commonly used by farmers in Bali, and observe the location of BCMV inside the seeds. The morphological observation was used to evaluate the percentage of BCMV brought by the seeds by observing BCMV specific symptoms appear on two weeks old long beans seedlings. To observe the location of BCMV inside the long bean seeds, harvested seeds from an infected plant were separated to collect the cotyledon and embryo. The RT-PCR technique was used as a method in this research. The result showed the percentage of BCMV brought by long beans seeds used by farmers in Bali categorized as high. The percentage of BCMV brought by seeds from the highest to the lowest were; variety A 48.39%, variety B 46.66%, variety C 43.59%, variety D 37.83%, variety E 22.86%, and variety F 22.72%. The variation of the symptoms was mosaic vein banding, malformation, and dwarf plant with wrinkled leaves. The BCMV brought by long bean seeds was resides on the embryo.
Efektivitas Trichoderma sp. yang Ditambahkan pada Kompos Daun untuk Pengendalian Penyakit Layu Fusarium pada Tanaman Stroberi (Fragaria sp.) di Desa Pancasari Kabupaten Buleleng SONIA ASHA HASARI; I GEDE RAI MAYA TEMAJA; I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Affectivity of Trichoderma sp. that Added in Leaf Compost to Control Fusarium Wilt Diseases on Strawberry Plants (Fragaria sp.) in Pancasari Village Buleleng RegencyThis research purpose in order to find the best concentrations of Trichoderma sp. which is added into the leaf compost to control fusarium wilt disease on strawberry plant. This research was conducted on December 2017 to March 2018 in Plant Diseases Laboratory of Udayana University and Pancasari Village, Sukasada District, Buleleng Regency. The experimental design used a Randomized Block Design (RBD) with six treatments and four replications, for each treatment consists of ten plant units. Observation variables in this research are disease percentage, Trichoderma sp. population on the ground, plant height, yield, and the weight of plants. The results showed that Trichoderma sp. treatment which were added into leaf compost is more effective to control the fusarium wilt diseases on strawberry plants rather than control treatments. P5 is the best treatment as it has the lowest disease percentage for around 20%, stimulate the highest growth of the plants into 25.8 cm, and has the highest yields 187 g.
Potensi Bakteri Endofit Dalam Menekan Pertumbuhan Phytophthora Palmivora (Butler) Secara In Vitro SUSAN CAMILA FOEH; I GEDE RAI MAYA TEMAJA; KHAMDAN KHALIMI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.4, Oktober 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Potential of Endophytic Bacteria in Suppressing The Growth of Phytophthorapalmivora (Butler) in Vitro The purpose of this study was to determine the potential of endophytic bacteria in controlling cocoa fruit rot caused by the fungus Phytophthora palmivora. The studywas conducted in vitro and direct test on cocoa fruit. The results of the inhibitory testshowed that 4 isolates of endophytic bacteria had the best inhibition for suppressingthe growth of Phytophthora palmivora. The in vitro inhibitory test showed, thetreatment of TN41 isolates was able to inhibit Phytophthora palmivora with thehighest inhibition percentage of 92.52% when compared to the control at theobservation of 5 days after inoculation. The direct test on cocoa fruit showed thetreatment of TN41 isolate had the least area of spot that is 0.20 mm2 at 7 days after inoculation. Further research needs to be conducted related to the stability ofendophytic bacteria isolate TN41 as an antagonist agent against Phytophthorapalmivora.
Kisaran Inang Bean Common Mosaic Virus (Bcmv) Penyebab Penyakit Mosaik Pada Tanaman Kacang Panjang (Vigna sinensis L.) A. A. GEDE PUTRA ADHITYA; I GEDE RAI MAYA TEMAJA; NI NENGAH DARMIATI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Host range of Bean Common Mosaic Virus (BCMV) causal agent of mosaic diseases on long yard bean  (Vigna sinensis L.) This objective of the study is order to find out the host range of BCMV and symptom variation. DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) menthod was used in this study. The study consisted of (1) Host range evaluation by mechanical inoculation, (2). Symptom observation on tested plants, (3) Serological test with DAS–ELISA. The result indicated that BCMV can infected Leguminoceae plants (peanuts, long yard beans, and beans); Solanaceae plant (tomatoes); Cucurbetaceae plant (cucumbers); Amaranthaceae plant (spinach); and Balsaminaceae plant (henna flowers). DAS-ELISA analysis shown that those plants were infected by BCMV. The symptoms variation were mosaic, vein banding,  wrinkled, and curved on commercial long yard beans (aura and panah merah varieties); wrinkled on commercial long yard beans (KPK and pusaka hijau varieties), cucumber and spinach; wrinkled and rolled on beans; wrinkled and curved upside on henna flowers.   Key word : bean common mosaic virus ,  long yard beans, DAS-ELISA
Identifikasi Virus Penyebab Penyakit Mosaik pada Tanaman Melon (Cucumis melo L.) di Bali RIZWAN FEBIANTA BRAHMANA; TRISNA AGUNG PHABIOLA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Viruses that Causes Mosaic Diseases in Melons(Cucumis melo L.) in Bali The aims of this research was to identify of some virus that infects melons in Bali. The first step of this research was to take samples in Jembrana Regency, TabananRegency, Gianyar Regency, Denpasar City, and Badung Regency. The next step wasdoing virus detection at Laboratory of Plant Diseases, Faculty of Agriculture, UdayanaUniversity. Variations of symptoms found in the field were mosaic, yellow leaves,malformations, and burning spots on the leaves. The percentage of virus attacks inGianyar and Badung was the highest percentage (60.00%), Jembrana (57.14%),Denpasar (42.85%), and Tabanan (37.50%). To know type of virus that causes thisdisease, molecular identification was conducted. Extraction was the first step to gettotal RNAs from symptomatic leaves. Total RNAs was transcribed intoComplementary (c) DNAs using reverse transctiption-polymerase chain reaction (RTPCR)technique. Polymerase chain reaction (PCR) was conducted using cDNA and three primer pairs of CABYV-489-F/Pol-G-R, MABYV-262-F/Pol-G-R andCIFOR/CIREV. Primers would be use to amplified a part of coat protein. CABYV489-F/Pol-G-Rand MABYV-262-F/Pol-G-R is specific primer, and CIFOR/CIREV would be use to amplified gene of Potyvirus. Based on the results of molecular with PCR it was found that melons in Bali were positively infected with viruses from thePotyvirus group with a size of around 683 bp in samples from Gianyar and Denpasar.
Deteksi Molekuler Cucumber Mosaic Virus (Cmv) pada Tanaman Gamal (Gliricidia Sepium) Sebagai Barier pada Pertanaman Cabai IDA BAGUS GDE PRANATAYANA; I GEDE RAI MAYA TEMAJA; KETUT YULIADHI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Molecular Detection of Cucumber Mosaic Virus (CMV) In Gliricidia Plant (Gliricidia sepium) as Barrier at Chili pepper Crop The aims of the study are in order to symptoms characteristic identity the CMV infection on gliricidia plant and symptoms characteristic the CMV infection on chili pepper plant, and order to presence identity the CMV on gliricidia plant and presence the CMV on chili papper plant at Kerta village base on molecular analysis. The serology method was used to detect CMV virus is DAS-ELISA (Double Antibody Sandwich-Enzyme Link Immunosorbent Assay) and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) was used for molecular analysis. The study include 1) the observation of mosaic in field, 2) preservation of plant samples (chili pepper and gliricidia) infected with mosaic virus, 3) serology test with DAS-ELISA, 4) molecular detection with RT-PCR. The research proved that symptoms characteristic the CMV infection on gliricidia plant identical with symptoms characteristic the CMV infection on chili pepper plant and presence the CMV on gliricidia plant identical with presence the CMV on chili pepper plant. symptoms of mosaic characteristic in gliricidia and chili pepper found on the field among others, mosaic (striped), contract and roll with variations of symptoms that light green mosaic with chlorotic and dark green mosaic symptoms in gliricidia and chili pepper plants. DAS-ELISA test results showed gamal and chili pepper samples infected positive of Cucumber Mosaic Virus (CMV). RT-PCR technique successfully amplify the target DNA fragment size of 657 bp. Based on these studies, the farmers need to be aware of the existence of the plant used as a plant barrier on chilli crop, because gliricidia potential as alternative hosts of CMV.   Key word : Chili pepper, cucumber  mosaic virus, gliricidia, molecular
The Observation of Blast Disease and Its Effect to Rice Yield Using Existing Assessment Method to Support the Indonesian Agriculture Insurance I Putu Sudiarta; I Komang Candra Giri Prayoga; I Gede Rai Maya Temaja; Gusti Ngurah Alit Susanta Wirya; Masahiro Shishido; Chiharu Hongo
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 15 No 2 (2021): Vol.15, No.2, 2021
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/SOCA.2021.v15.i02.p15

Abstract

One of the causes for the low productivity of rice in Indonesia is the occurrence of rice blast disease. Blast disease is one of the main diseases of rice plants around the world. Therefore to minimise the risk of the lost yield by blast disease, the Ministry of Agriculture of the Republic of Indonesia has the agriculture insurance program. To support these program, the research about observations of blast disease in a fixed location are needed. The objective this research is to confirm pathogens causing the blast disease in the fields, to determine the development of blast disease and its effect to the rice yield (grain weight and straw weight). The study was conducted in a fixed paddy field at Subak Uma Dalem, Sobangan Village, Mengwi District, Badung Regency, Bali Province from February 2019 to April 2019. The data showed that the disease incidence of rice blast increased every week starting from 1st week (28 DAT) until 10th week (104 ATP). Our study confirmed that the fungus caused the blast disease in the field was Magnaporthe oryzae. The higher incidence of blast disease in the field resulted reduced the rice yield by decreasing both grain and straw weights.
Co-Authors A. A. GEDE PUTRA ADHITYA Abd. Rasyid Syamsuri Ayu Rahmawatiningsih Chiharu Hongo Debora Margareth Dewa Ngurah Suprapta Dewa Nyoman Darmayasa G. SUASTIKA Gede Bayu Rahanatha GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Gede Rian Pramarta I Komang Candra Giri Prayoga I Made Angga Prayoga I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDARMA I Made Sukewijaya I NYOMAN RAI I Putu Sudiarta I PUTU WIRYA SUPUTRA I WAYAN WIKA CENDANA PUTRA IDA AYU ASTARINI IDA BAGUS GDE PRANATAYANA Ifa Manzila KETUT AYU YULIADHI Khairun Nisak Syahdu Khamdan Khalimi l MADE SUDARMA LASTRI APRIANI M SUDANA M. Sudiana Mahendra Masahiro Shishido Muhammad Ikhsan Nulzaen Ni Luh Gde Sumardani Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Dwi Fatmawati Ni Nengah Putri Adnyani Ni Nyoman Alit Purwaningsih NI NYOMAN ARI MAYADEWI Ni Nyoman Kerti Yasa Ni Putu Widyami Yanthi Nyoman Bintang Kartika Sari RIZWAN FEBIANTA BRAHMANA Ruimassa, Reymas Sari Nurulita SH. HIDAYAT SONIA ASHA HASARI SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA U. KARTOSUWONDO Valente Quintao Wahyu Hidayati Yuyun Fitriani