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All Journal Jurnal Fitopatologi Indonesia IPTEKMA SOCA: Jurnal Sosial Ekonomi Pertanian AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology Bumi Lestari Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan Cropsaver : Journal of Plant Protection
I G. R. M. TEMAJA
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Medicine & Pharmacology

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PENGENDALIAN PENYAKIT VIRUS PADA TANAMAN CABAI RAWIT (CAPSICUM FRUTESCENS L.) DENG¬AN MULSA PLASTIK HITAM DAN PERAK Dewa Nyoman Darmayasa; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The research of virus diseases management in chili pepper plant (Capsicum frutescens L.) by utilizing black and silver plastic mulch, was conducted in order to find the capability of black and silver plastic mulch in controlling virus diseases in chili pepper plant and also to find the capability of black and silver mulch in increasing yield of chili pepper plant. This research used randomized block design with 3 treatments and 9 replications. The result of the research shows that utilization of silver plastic mulch had ability to control virus diseases indirectly. The yield of chili pepper has incrising up to 143% with silver plastic mulch treatments.
STRATEGI PENGENDALIAN PAPAYA RINGSPOT VIRUS (PRSV) PENYEBAB PENYAKIT BERCAK BERCINCIN PADA PEPAYA I Gede Rai Maya Temaja; I Putu Sudiarta; Ni Nengah Darmiati; Ni Made Puspawati
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

ELISA test results of symptomatic leaves from Tabanan, Bali showed that papaya ringspot virus (PRSV) as causing agents of ring spot disease on papaya in Bali. The objective of the study were to determine the control strategies of ringspot disease on papaya in the field. Control strategies of ringspot disease tested in this study were the use of metallic gray plastic mulch and plant barrier. Research result showed that plant barrier can provide a buffer zone to protect papaya that are planted within the confines of the buffer.
UTILIZATION OF SDT-RT-PCR FOR PLANT VIRUS DETECTION I Gede Rai Maya Temaja; Ni Made Puspawati; Ni Nyoman Ari Mayadewi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Metode simple-direct-tube (SDT) dikembangkan untuk preparasi     RNA virus atau viroid, yang selanjutnya dipakai untuk sintesis cDNA. SDT tidak memerlukan penyaringan atau sentrifugasi seperi pada ekstraksi RNA total menggunakan KIT. Metode ini juga tidak menggunakan antiserum dalam preparasi RNA virus. Di samping itu metode SDT dapat dikerjakan di laboratorium dengan temperatur ruang dan selesai dikerjakan lebih kurang dalam 25 menit. Penerapan metode ini dilanjutkan dengan reverse transcription-polymerase chain reaction (RT-PCR), dapat mendeteksi keberadaan tomato chlorosis virus (ToCV), tomato infectious chlorosis virus (TICV), chrysanthemum virus B (CVB), Potyvirus and chrysanthemum stunt viroid (CSVd). Hal ini menunjukkan bahwa metode SDT dapat dipakai untuk mendeteksi virus atau viroid.
Pemanfaatan Rizobakteri Penghasil Indole Acetic Acid Sebagai Agens Penginduksi Ketahanan Tanaman Tembakau Terhadap Cucumber Mosaic Virus (CMV) Ni Luh Gede Sriyanti; Khamdan Khalimi; I Gede Rai Maya Temaja
Journal of Agricultural Science and Biotechnology Vol 8 No 1 (2019)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Tobacco is a high economic value commodity, in order to meet the demand for tobacco in the market it requires good quantity and quality. One of the diseasethat often causes harm to tobacco is Cucumber Mosaic Virus (CMV). The utilization of indole acetic acid (IAA)-producing rhizobacteria can be used to increase plant growth and resistance from pathogenic infection. The aim of this study was to determine the effectiveness of IAA producing rhizobacteria formula in increasing the growth of tobacco plants in greenhouses, and to determine the effectiveness of rhizobacterial formulations in increasing tobacco plant resistanceagainst the infection of CMV. Identification of compounds in tobacco leaves that infected with CMV was carried out to determine the effectiveness of rhizobacteria in inducing plant resistance. Compound analysis was carried out using Gas Mass Chromatography Spectroscopy (GCMS). The effectiveness of rhizobacteria in increasing plant growth and resistance was conducted in a greenhouse. Based on the results of the study, four of IAA-producing rhizobacteria isolates were Stenotrophomonas maltophilia Sg3, Proteus mirabilis BjB17, Bacillus thuringiensis TNJbx3.3, and Bacillus cereus GR12, all the rhizobacterial isolates were effective in increasing plant height, leaf number, leaf chlorophyll content, wet weight and dry weight leaf. Based on the results of the rhizobacterial effectiveness test, all rhizobacterial isolates were effective in increasing plant resistance as indicated by a lower intensity of infection. Based on the analysis of the tobacco leaves compounds content showed that rhizobacteria S. maltophilia Sg3 and P. mirabilis BjB17 were able to induce resistance to tobacco plants by forming antiviral compounds namely 2-Naphthalene-sulfonic acid and rhizobacteria Bacillus cereus GR12 can stimulate the formation of 1-Naphthalene-sulfonic acid compounds and 2-Naphthalenesulfonic acidin tobacco as a form of induction of resistance to CMV infection.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN PADI SEBAGAI AGEN HAYATI UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Pyricularia oryzae, PENYEBAB PENYAKIT BLAS PADA TANAMAN PADI Valente Quintao; Dewa Ngurah Suprapta; I Gede Rai Maya Temaja; Khamdan Khalimi
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oryza sativa L.) is one of the staple foods for more than 60 percent of world population. Indonesian people generally are still depending on the availability of rice to fulfill Indonesian food demand. The average of rice productivity in Indonesia is 4.56 ton/ha which is relatively lower when compared with other rice growing countries, such as Australia 8.22 ton/ha;  Japan 5.85 ton/ha and China 6.06 ton/ha. One of the causes for the low productivity of rice in Indonesia is the occurrences of rice blast disease caused by Pyricularia oryzae.  To control this disease, the farmers are still rely on the use of synthetic fungicides however this measure can not effectively control the disease,  and potentially cause the health and environmental problems. It is necessary to find an alternative measure  that save to human health as well as friendly to the environment. This study was  done to evaluate the potential use of rhizobacteria isolated from rhizospheres of rice as antagonist against Pyricularia oryzae the  cause of rice blast disease.  The result showed that five isolates of rhizobacteria namely  Xanthomonas lumininescens isolate Ch3Da, Serratia liquefaciens isolate Gh13DaB, Enterobacter agglomerans isolate Gg14DtB, Enterobacter agglomerans isolate Ch2Da, and Enterobacter agglomerans isolate Ch4BaB significantly (P<0.05) inhibited the growth of Pyricularia oryzae on potato dextrose agar (PDA) medium with inhibitory activity varied from 39.46% to 46.66%. All of these isolates produced extra cellular substances which probably responsible for the inhibitory activity. This result suggested that five isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of rice blast disease.
BAKTERI PELARUT FOSFAT MEMICU KETAHANAN TANAMAN KEDELAI (Glycine max) TERHADAP PENYAKIT SOYBEAN MOSAIC VIRUS Ni Nyoman Alit Purwaningsih; I Dewa Nyoman Nyana; I Gede Rai Maya Temaja; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean (Glycine max) is an important commodity in Indonesia. The high utilization of soybean has not been accompanied by the sufficiency of amount of its production, even decreased from year to year. One of the factors that cause the decreased of soybean productivity is soybean mosaic disease caused by viral pathogens. Soybean mosaic virus is one of the most important types of virus in soybean could cause a decreased in yield until 25-90%.This study aimed to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to providing resistance to SMV disease on soybean plant. Six isolates rhizobacteria that could be isolated from Leguminosae plants had used as a treatment in the test. The result of field test showed three isolates namely R13, R12, and R9 able to increase soybean plant resistance, so each isolates made influence to crop yield. The results of identification based on the analysis of 16S rRNA gene, isolate R13 which gave the best response to soybean plant resistance and had percentage similarity between 94-98% with species Empedobacter brevis (Flavobacterium breve).
KARAKTERISTIK FENOTIF ISOLAT KLINIK Escherichia coli O157:H7 PADA MEDIA SORBITOL MAC CONKEY AGAR (SMAC) Wahyu Hidayati; I Gede Rai Maya Temaja; Ni Nengah Dwi Fatmawati
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Phenotypic Characteristic of Escherichia coli O157: H7 Clinical Isolates on MacConkey Agar with Sorbitol (SMAC)Escherichia coli is a Gram-negative, rod-shaped bacterium, and commonly has flagella. Most of E. coli strains are normal flora in digestive tract of human, but some serotypes are pathogen for human and animal. One of pathogenic E. coli strain that causes a severe infection in humans (hemorrhagic colitis and hemolytic uremic syndrome) is known as Enterohemorrhagic Escherichia coli (EHEC) with cattle as their natural reservoir. E. coli O157:H7 is the most important and pathogenic serotype of EHEC which responsible for outbreak of Hemorrhagic Colitis and Hemolytic Uremia Syndrome. Accurate and cheap method detection of E. coli O157:H7 is needed to help early detection diagnosis and therapy. Therefore, the aim of this study is investigate phenotypic characteristics of E. coli O157:H7 isolated from clinical specimens using MacConkey Agar with Sorbitol (SMAC) media. Three E. coli clinical isolates from Sanglah General Hospital showed colorless non-sorbitol fermenting colony in SMAC media as phenotypic characteristic of E. coli O17:H7, therefore SMAC may beused on are of confirmation methods for E. coli O157:H7 detection from clinical isolats.
IDENTIFIKASI SPESIES POTYVIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI (Capsicum frutescens L.) MELALUI SIKUEN NUKLEOTIDA GEN Coat Protein I Gede Rian Pramarta; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Chili peppers (Capsicum frutescens L.) is one of the important vegetable in the world and one of the leading horticultural commodities in Indonesia. Pepper plants grown in all provinces in Indonesia and got priority for development because it has great potential economic value. These researches was conducted in order to determine the type of infecting Potyvirus on chili and know the proximity nucleotide sequence of Potyvirus infecting chili crop in Kerta village, Payangan, Gianyar, Bali, with the kind of Potyvirus in other areas. This study use RT-PCR technique to detect plant viruses and continued with tracing sequence coat protein gene nucleotide to its proximity to some of the isolates analyzed from GeneBank. The results showed that the infecting Potyvirus chili crop is Chili veinal motle virus species (ChiVMV) with size of approximately 900 bp DNA band, and based on phylogenetic analysis of coat protein gene nucleotide sequence, PayanganIndo isolate had high similarity (93%) and small genetic distance (0.041) with isolate of origin Thailand state.
BIOEKOLOGI Grapevine leafroll-Associated Virus type 3 (GLRAV-3) PENYEBAB PENYAKIT DAUN MENGGULUNG PADA TANAMAN ANGGUR (Vitis vinifera L.) Yuyun Fitriani; I Gede Rai Maya Temaja; G. N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The survey of vineyard fields in Buleleng, Bali, Indonesia was conducted, some grapevine cultivars were found had grapevine leaf roll disease symptomps. The symptoms including interveinal reddening or yelowing and fall leaves, down rolling of leaves, phloem disruption, and the fruit maturation is delayed. The analysis of enzyme linked immunosorbent assay (ELISA) of symptom leaf with Grapevine leaf roll-associated virus type 3 (GLRaV-3) antiserum shown the disease was corresponding to the GLRaV-3. The study was conducted to identify the mealybug that colonized of grapevine, to determine capability of mealybug to transmitted GLRaV-3 and host range GLRaV-3.Base on the survey ofmealybuginvineyard fields in Buleleng, Bali, the two types of mealybug were identified, namely Ferrisia virgata (Ckll.) (Hemiptera: Pseudococcidae) and Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). Results of the transmission test showed that only M. Hirsutus are capable of transmitting GLRaV-3. The plant range host test of GLRaV-3 inoculation with M. hirsutuswas conducted and the symptom was detected on3plants species, grapevines (Vitis vinifera), native gooseberry (Physalis floridana) andshoe flower (Hibiscus rosa-sinensis) from18 species of plants that used.
APLIKASI POLYMERASE CHAIN REACTION-RIBOSOMAL INTERGENIC SPACER ANALYSIS (PCR-RISA) UNTUK MENENTUKAN KERAGAMAN MIKROBA TANAH PADA HABITAT TANAMAN PISANG DENGAN DAN TANPA GEJALA LAYU FUSARIUM I Made Sudarma; Dewa Ngurah Suprapta; I Made Sudana; I Gede Rai Maya Temaja
Bumi Lestari Journal of Environment Vol 12 No 2 (2012)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A major problem in the soil microbiology is 99% soil microbes could not be grown in artificial media; therefore, to describe the true diversity of microbes in the soil must use a molecular approach. The approach was polymerase chain reaction-ribosomal intergenic spacer analysis (PCR-RISA). This method can be used for determining soil microbial DNA profile (the number of DNA bands). The soil samples were collected from three regencies in Bali,  i.e. Karangasem, Klungkung and Jembrana which are the main banana growing area in Bali. Soil sampling was done in two sites in each regency representing the banana habitat with and without Fusarium wilt symptom, by collecting 100 grams of soil surrounding the banana plant at the depth of 20 cm. The result of PCR-RISA showed that DNA profile of the soil microbes in the soil of banana habitat without Fusarium wilt symptom (HN) more than of the soil banana habitat with Fusarium wilt symptom (HF). DNA profiles at HN there were three, each of the indicated 450 bp, 250 bp and <100 bp, whereas in HF were two bands of DNA, each indicated as 250 bp and <100 bp. Based on the number of DNA bands proved that the soil microbial community in the HN is higher than in HF.
Co-Authors A. A. GEDE PUTRA ADHITYA Abd. Rasyid Syamsuri Ayu Rahmawatiningsih Chiharu Hongo Debora Margareth Dewa Ngurah Suprapta Dewa Nyoman Darmayasa G. SUASTIKA Gede Bayu Rahanatha GEDE SUASTIKA Gede Suastika Gede Suastika Gede Suastika Gusti Ngurah Alit Susanta Wirya I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Dewa Nyoman Nyana I Gede Rian Pramarta I Komang Candra Giri Prayoga I Made Angga Prayoga I Made Sudana I MADE SUDANA I Made Sudana I MADE SUDARMA I Made Sukewijaya I NYOMAN RAI I Putu Sudiarta I PUTU WIRYA SUPUTRA I WAYAN WIKA CENDANA PUTRA IDA AYU ASTARINI IDA BAGUS GDE PRANATAYANA Ifa Manzila KETUT AYU YULIADHI Khairun Nisak Syahdu Khamdan Khalimi l MADE SUDARMA LASTRI APRIANI M SUDANA M. Sudiana Mahendra Masahiro Shishido Muhammad Ikhsan Nulzaen Ni Luh Gde Sumardani Ni Luh Gede Sriyanti Ni Luh Putu Citra Innosensia NI LUH PUTU CITRA INNOSENSIA Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Dwi Fatmawati Ni Nengah Putri Adnyani Ni Nyoman Alit Purwaningsih NI NYOMAN ARI MAYADEWI Ni Nyoman Kerti Yasa Ni Putu Widyami Yanthi Nyoman Bintang Kartika Sari RIZWAN FEBIANTA BRAHMANA Ruimassa, Reymas Sari Nurulita SH. HIDAYAT SONIA ASHA HASARI SUSAN CAMILA FOEH TRISNA AGUNG PHABIOLA U. KARTOSUWONDO Valente Quintao Wahyu Hidayati Yuyun Fitriani