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Journal : agriTECH

Aktivitas Fitase Pada Tahap-Tahap Pembuatan Tempe dari Kara Benguk, Gude, dan Kara Putih Menggunakan Usar Tranggono Tranggono; Sutardi Sutardi; Meta Mahendradatta
agriTECH Vol 11, No 4 (1991)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2236.645 KB) | DOI: 10.22146/agritech.19226

Abstract

Penelitian dilakukan untuk mengkaji perubahan aktivitas fitase selama proses pembuatan tempe kara benguk, gude dan kara putih dengan menggunakan usar. Ekstrak kasar fitase dipersiapkan metalui beberapa tahap isolasi dan pemurnian, sedangkan aktivitas Masa diukur berdasarkan jumlah fosfat anorganik yang dibebaskan dari substrat natrium fitat pada kondisi pengujian yang ditetapkan. Suhu dan pH optimum untuk fitase kara benguk dan kara putih berturut-turut 60°C clan 4,8 sedangkan untuk gude berturuHurut 50°C dan 5.0. Hasil yang diperoleh menunjukkan penurunan aktivrtas fitase selama perlakuan perendaman pada kara benguk dan kara putih, berturul-turul dan 129.56- 48.73 μ mol dan 169,30-77.92 μ mol. Pada gude tidak ditakukan pengujian aktivitas fitase selama perlakuan perendaman, Pada tahap fermentasi 0 - 24 jam. terjadi kenaikan aktivitas fitase berturut-turut dari 0 - 193,23 μ mol, 0 - 96.24 μ mol dan 5,56-46,33 μ mol untuk kara benguk, gude dan kara putih. Setelah 24 jam ferrnentasi, aktivrtas fitase turun menjadi berturut-turut sebesar 60,72 μ mol. 59.56 μ mol dan 7.68 μ mol. Selama fermentasi 36 - 48 jam, aktivitas fitase naik lagi berturut-turut sebesar 164,76 μ mol, 118,59 μ mol dan 9,27 μ mol untuk kara benguk. gude dan kara putih. Selama proses pembuatan tempe kandungan asam fitat turun berturut-turut dari 0,37 - 0,14%, 0,42 - 0,10% dan 2,26 - 0.16% masing-masing dalam berat kering untuk kara benguk, gude dan kara putih,
The Physicochemical Characteristics of Palado Seed Flour (Aglaia sp.) and Its Potential Use as a New Alternative Flour in Indonesia Syamsul Rahman; Salengke Salengke; Abu Bakar Tawali; Meta Mahendradatta
agriTECH Vol 42, No 3 (2022)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.779 KB) | DOI: 10.22146/agritech.63255

Abstract

Flour is one of the main energy sources for humans and an important ingredient in food processing, for use as fillers, thickeners, gel, film forming and adhesives. Therefore, this study aimed to analyze the physicochemical characteristics of palado seed flour (Aglaia sp.) including proximate analysis and chemical properties comprising energy, carbohydrates, water, and protein, content, as well as dietary fiber, starch, amylose and amylopectin. Furthermore, the physical properties including yield, particle size, degree of whiteness, paste clarity, gel strength, swellability and water absorption, as well as the potential use of palado flour (Aglaia sp) in food processing were analyzed. The study was conducted with a method comprising two stages, namely pre-treatment including processing palado fruit into seeds, drying, and extracting flour, as well as proximate analysis of the physicochemical characteristics. The results showed energy content of 396.14 kcal/100 g, carbohydrates 69.78%, water content 7.53%, protein 9.59%, fat 8.74%, dietary fiber 4.02. %, ash 4.36%, starch 41.31%, amylose 5.68% and amylopectin content 35.63%. Meanwhile, the physical characteristics include the yield of 84.21% with a particle size of 60 mesh, degree of whiteness 29.27%, paste clarity 29.7%, gel strength 109.96 gf, swelling power 0.4%, and water absorption 133.72 %. Based on the results, palado flour has high potential to be developed as the main or alternative ingredient in bread and noodle processing, such as durian and jackfruit seed flour.