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FTIR Profiles, Proximate Composition, and Microbial Counts of Tauco with Sensory and Amino Acid Profiles of Ultrafiltration Fractions Herlina, Vika Tresnadiana; Lioe, Hanifah Nuryani; Adawiyah, Dede Robiatul; Kusumaningrum, Harsi Dewantari
Indonesian Food Science and Technology Journal Vol. 9 No. 1 (2025): Volume 9 Number 1, December 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v9i1.43977

Abstract

Twenty-four different tauco samples, available as commercial salt-fermented soybean products in Indonesia, were investigated for their microbial counts, proximate composition, as well as FTIR profiles obtained by attenuated total reflectance-fourier transform infrared (FTIR-ATR) instrument. The total and free amino acid profiles by UHPLC, as well as sensory characteristics by taste dilution analysis (TDA) of their ultrafiltration fractions (tauco fractions with molecular weights less than 3 kDa or <3 kDa) were also revealed. The objective of this study was to comprehensively characterize the commercial tauco products in Indonesia through integrated microbial, chemical, and sensory analyses of the products and their low molecular fractions. Tauco samples had total microbes reaching 4 to 9 log CFU/g. Lactic acid bacteria (LAB), which act in the second step of fermentation, were counted at much higher levels in all samples than molds/yeasts, which are important in the first step of fermentation. FTIR profiles of tauco samples are correlated with their protein content. Functional groups correlated with the proximate composition of tauco are identified as follows: protein with carbonyl (C=O) and aliphatic hydrocarbon (C-H), fat with carbonyl (C=O), and carbohydrate with hydroxyl (O-H) groups. Umami was identified as the predominant taste of their ultrafiltration fractions. The amino acid profiles of tauco fractions <3 kDa showed relatively high residual amino acid contents, suggesting abundant peptide content. However, multivariate orthogonal projections to latent structure (OPLS) analysis showed that free amino acids, including glutamic and aspartic acids (umami), glycine and serine (sweet), and phenylalanine and tyrosine (bitter), contributed significantly to the umami characteristic of tauco. This finding shows that free amino acids could contribute to the desirable taste of tauco. This study provides a comprehensive characterization of Indonesian tauco, offering a practical scientific reference for producers to understand product variability, improve fermentation control, and enhance the consistency and value of commercial products.
Co-Authors Agustin, Denny Ahmad Johari Akbar, Dzifiar Yasykuri Akbar, Dzifiar Yasykuri Alfiyani, Novi Amalia Rakhmadani Amalia Shabrina Ramadhani Anggrei Viona Seulalae Angka, Stephanie Anis Khairunnisa, Anis Annisa Hayyu Fatmawati Astri Hermeinasari Ayunda Rachmawati Azis Boing Sitanggang Bambang Pontjo Priosoeryanto Bendi Sabela Budi Nurtama C Hanny Wijaya Dahrul Syah Dase Hunaefi Dase Hunaefi Denny Agustin Desty Gitapratiwi Dewi Sartika Saragih Dian Herawati Dian Puspitasari Dias Indrasti Dias Indrasti Dwinawati, Elisabeth Efendi, Darda Eko Purnomo Eko Purnomo Eko Yuniarsih Elvira Syamsir Endang Prangdimurti Erniati Erniati Erniati, Erniati Febby Setiawan Felia Prima Wefiani Feri Kusnandar Florentina Maria Titin Supriyanti Fransisca Zakaria Rungkat Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria FRANSISKA RUNGKAT ZAKARIA Fransiska Utami Frido, Salsabila Safira Gitapratiwi, Desty Hanifah Nuryani Lioe Henni Rizki Septiana Herlina, Vika Tresnadiana Hermeinasari, Astri Hoerudin Hoerudin Hoerudin Hoerudin Hunaefi, Dase Kaoru Kohyama Kariska Iswari Yasa Kezia Patricia Krisjati, Paulina Gandhes Dian Leonie Margaretha Widya Pangestika, Leonie Margaretha Widya Liber Liber Liliasari , Lilis Nuraida Lince Lince Lince Lince Lince Lince Malik Abdul Azis Maria Afrida Masykur, Siti Fauziyyah Medina Alia Rahmawati Melina Melina Melzer, Guido MINTARSIH Mirlanda Miss Sathita Wisetsombat Mutiara Utami N. Nurjanah Namirah, Aisyah Maryam Ni Nyoman Puspawati Nova Kurnia Nugraha Edhi Suyatma Nur Wulandari Nur Wulandari Nur Wulandari Nuri Andarwulan Nurjanah Nurjanah Nurlela, Lani Olivia Mellyana Tjiptoputri Palupi, Nurheni Sri Patricia, Kezia Piyaporn Chueamchaitrakun Piyaporn Chueamchaitrakun Purwiyatno Hariyadi Putri, Permata Adinda Ramlan, H. Reno Irwanto Ria Noviar Triana Rinaldi, Ahmad Rini Kesenja Rini Kesenja Rohani Islami SARASWATI SARASWATI Sari, Ulfiana Anika Sholehuddin, Zen Fauzan Siregar, Lerissa Aulia Siti Fauziyyah Masykur Stephanie Angka Subarna Subarna Sugiyono . Sugiyono Sugiyono Suliantari Suliantari Tiara Indra Saraswati Tjahja Muhandri Tomoko Sasaki Triana, Ria Noviar Ulfiana Anika Sari Uswatun Hasanah Utami, Mutiara Siska Putri Weinreich, Bernd Winiati Pudji Rahayu Yogi Karsono Yulianti Yulianti Zeller, Yves Henri Marie