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Kualitas Makroskopis Semen Ayam Kampung yang Diberi Ekstrak Kulit Buah Naga Dhian Ramadhanty; Angga Nugraha; Nurul Purnomo; Andi Fausiah
AGROVITAL : Jurnal Ilmu Pertanian Vol 6, No 1 (2021): Agrovital Volume 6, Nomor 1, Mei 2021
Publisher : LPPM Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/agrovital.v6i1.2005

Abstract

Motilitas dan Abnormalitas Spermatozoa Ayam Kampung yang Diberi Suplementasi Ekstrak Kulit Buah Naga Dhian Ramadhanty; Angga Nugraha; Nurul Purnomo; Armayani M
JURNAL GALUNG TROPIKA Vol 10 No 1 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i1.703

Abstract

This study aimed to determine the effect of dragon fruit peel extract on the motility and abnormalities of native chicken spermatozoa. The study was arranged experimentally using a completely randomized design consisting of 4 treatments and 3 replications. Before treatment, 12 native chicken were randomly divided into groups and adapted for 10 days with commercial feed and drinking water. The treatments were given orally for 8 weeks and consisted of P0 (control), P1 (1 ml dragon fruit peel extract), P2 (3 ml dragon fruit peel extract), and P3 (5 ml dragon fruit peel extract). Cement storage is carried out by the sequencing method. The quality of the collected sperm is tested in the laboratory. The parameters measured were microscopic sperm quality, namely sperm motility, and abnormalities. The results showed that dragon fruit peel extract had a significant effect on spermatozoa motility by increasing the percentage of motility but had no effect on spermatozoa abnormalities. Provision supplement of 5 ml dragon fruit peel extract gave the best results in increasing spermatozoa motility in native chickens.
KUALITAS DNA HASIL PURIFIKASI DARI SOSIS SAPI SEBAGAI BAHAN AUTENTIKASI HALAL BERBASIS MARKER GENETIK Nurul Purnomo; Dhian Ramadhanty; Musdalifa Mansur; Maghfira Nur; Muhammad Ihsan A. Dagong
Jurnal Ilmu dan Teknologi Peternakan Vol. 9 No. 1 (2021)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v9i1.11633

Abstract

This study aims to determine the effect of the preparation method and the number of samples on the quality of purified DNA from beef sausage. The study was conducted using a Randomized Block Design. The main factor is the sample preparation method which consists of P1: fresh sample; P2: oven-dried sample; and P3: Freeze-drying dry samples. Each treatment consisted of 4 groups, namely K1: sample weight 25 mg; K2: sample weight 50 mg; K3: sample weight 75 mg; and K4: sample weight 100 mg. Each treatment group was repeated 2 times so that the total sample was 24. The results showed that DNA purification using different preparation methods and sample weights was successfully carried out, as seen from the presence of DNA bands visualized on agarose gel with EtBr staining. The average DNA quality of the P1, P2, and P3 preparation methods were 1.60±0.90 ng/μl, 2.63±0.99 ng/μl, and 2.94 ±0.89, ng/μl respectively, with DNA purity 1.023 ±0.165, 0.937±0.148, and 1.014±0.163. The average DNA quality at K1, K2, K3, and K4 obtained DNA concentrations of 3.03±1.64 ng/μl, 3.15±0.74 ng/μl, 2.28± 1.66 ng/μl, and 2.54±1.50 ng/μl with a purity of 1.059±0.142, 0.981±0.130, 0.908±0.061, and 1.061± 0.215. The average total concentration of purified DNA from beef sausage was 2.75±1.28 ng/μl with a purity of 0.991±0.149. The results of variance showed that the treatment did not affect the concentration and purity of purified DNA from beef sausage. This study concludes that DNA purification from beef sausage can be carried out, but the preparation method and number of samples do not affect the quality and quantity of DNA.
Pengaruh Suplementasi Ekstrak Daun Kelor (Moringa oleifera) Terhadap Kualitas Makroskopis Spermatozoa Ayam Kampung Dhian Ramadhanty; Nurul Purnomo; Andi Fausiah; Dwi Maryana
AGROVITAL : Jurnal Ilmu Pertanian Vol 7, No 1 (2022): AGROVITAL VOLUME 7, NOMOR 1, MEI 2022
Publisher : Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/agrovital.v7i1.2692

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh suplementasi ekstrak daun kelor (Moringa oleifera) terhadap kualitas makroskopis spermatozoa ayam kampung. Penelitian ini menggunakan 15 ekor ayam kampung jantan berumur + 1 tahun dibagi secara acak dalam 3 kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Sebelum diberi perlakuan, seluruh ayam diadaptasikan selama 1 minggu dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral pada pagi hari selama 45 hari dan terdiri dari P1 : Ekstrak Daun Kelor 3 ml, P2 : Ekstrak Daun Kelor 6 ml, dan P3 : Ekstrak Daun Kelor 9 ml. Setelah dipelihara dan diberi perlakuan selama 45 hari, dilakukan penampungan sperma. Teknik penampungan sperma pada ayam dilakukan dengan menggunakan metode massage (metode pemijatan) pada bagian punggung ayam. Sperma yang telah ditampung kemudian diperiksa kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas sperma secara makroskopis meliputi volume, pH, warna, konsistensi, dan bau sperma. Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak daun kelor tidak berbeda nyata terhadap volume, pH, warna, konsistensi, dan bau sperma (P>0,05) tetapi seluruh kualitas sperma yang diperoleh setelah pemberian  ekstrak daun kelor termasuk dalam kategori yang normal dan baik.
Kandungan Nutrisi Daun Gamal Sebagai Pakan Ternak Ruminansia pada Ketinggian Lokasi Berbeda: Nutritional Content of Gamal Leaves as Ruminant Animal Feed at Different Location Altitudes Nurul Purnomo; Inayah, Nurul; Selvin, Agustina; Sahrul, Muhammad; Faisal Saade, Muhammad
Jurnal Agrisistem Vol. 20 No. 2 (2024): Jurnal Agrisistem
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Pembangunan Pertanian Gowa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52625/j-agr.v20i2.406

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan nutrisi daun Gamal sebagai pakan ternak ruminansia pada ketinggian lokasi yang berbeda. Penelitian ini dilakukan menggunakan data primer dan data skunder. Data primer diperoleh dengan melakukan analisa kandungan nutrisi daun Gamal dari kota Makassar yang memiliki ketinggian lokasi 8 mdpl menggunakan metode analisa Proksimat. Data skunder diperoleh dari hasil penelitian yang dilakukan pada lokasi dengan ketinggian 244 mdpl dan 456 mdl. Hasil penelitian menunjukkan bahwa kandungan bahan kering daun Gamal cenderung meningkat seiring peningkatan lokasi, kandungan bahan organik, relatif sama pada ketiga ketinggian lokasi, kandungan protein kasar cenderung meningkat pada lokasi yang lebih tinggi, kandungan lemak kasar relatif sama pada ketiga ketinggian lokasi, kadar serat kasar relatif sama pada ketiga ketinggian lokasi dan kadar BETN relatif sama pada ketiga ketinggian lokasi, dan kadar abu relatif sama pada pada ketiga ketinggian lokasi. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa kandungan bahan kering dan protein kasar daun Gamal cenderung meningkat pada lokasi yang lebih tinggi, tetapi kandungan bahan organik, lemak kasar, serat kasar BETN, dan abu relatif sama.
The Dynamics of Farmer Motivation in Cattle Breeding: A Case Study of Mario Marennu Cattle Group, Kulo District Baharuddin, Dewi; Nugraha, Angga; Purnomo, Nurul
Tarjih : Agribusiness Development Journal Vol. 4 No. 02 (2024): VOLUME 04, NOMOR 02, DESEMBER 2024
Publisher : Program Studi Agribisnis Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/tadj.v4i02.820

Abstract

Farmer motivation is critical in increasing the productivity and sustainability of livestock businesses, especially in rural areas. This study aims to identify the level of farmer motivation in Mario Village, Kuro District, and Sidenreng Rappang Regency and analyze the factors that influence it. With a quantitative descriptive approach and multiple regression analysis using SPSS 16.0 software, this study provides an in-depth description of the relationship between age, education, and livestock farming experience factors on farmer motivation locally. This study aimed to determine the level of farmer motivation in Mario Village, Kulo District, Sidenreng Rappang Regency, and the factors that influence this motivation. The method used was quantitative descriptive, and the sample consisted of 63 respondents. Data were collected through questionnaires and analyzed using multiple regression using SPSS 16.0 software. The results showed that two main factors significantly influenced farmer motivation: age and livestock farming experience. This can be seen from the regression coefficient of age (X1) reaching 0.500 and farming experience (X2) reaching 0.320. These values show a significant value of less than 0.05, indicating that they significantly influence farmer motivation
Strategi Pencapaian Kesetaraan Gender Dalam Rumah Tangga Nelayan Muhammad Bibin; Nurul Purnomo; Ani Ardian; Sry Iin Indirwan; Saman Gita Marewa
Jurnal Ilmu Sosial dan Humaniora Vol 13 No 3 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jish.v13i3.82538

Abstract

Decision-making in a household should not be done single-handedly by the husband as the head of the family. However, it needs to involve the wife, who plays an important role in improving the family economy. Generally, the women or wives in Palopo had not been involved in decision-making and had not been fully supported by government programs taking sides with the women. This study sought to analyze gender equality of decision-making in fisherman households and formulate a priority strategy for achieving gender equality in these households. Data collection was done using observation and structured interviews using questionnaires addressed to 7 stakeholders consisting of government agency officials, the Chair of Fisherman Cooperative, the Head of Nasyiah, and the Head of the Fisherman Group, as well as 30 families (husband and wife) whose livelihood depended in the fishery sector. The Moser analysis and Analytical Hierarchy Process (AHP) were used to analyze the obtained data. This study revealed some results. The women or wives were responsible and more dominant as decision-makers in household, family, and financial matters. The men or husbands were responsible and more dominant decision-makers in production activities and social affairs. In addition, implementing outreach programs for coastal communities in Palopo had not been gender-responsive. Thus, the priority strategy in achieving gender equality in fisherman households, which holds the best opportunity and is considered important by stakeholders, is developing a guidance program for coastal communities, men, and women.
Study on The Production of Local Microorganisms (MOL) as a Starter for Cattle Feed Fermentation Aisyah, Nurul; Irwan, Muh; Purnomo, Nurul
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 11 No 1 (2025): Juni
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v11i1.224

Abstract

This study aims to determine the impact of various carbohydrate sources on the temperature, liquid volume, color, and pH of local microorganisms (MOL). The research employed a descriptive and quantitative approach, using a completely randomized design (CRD) with three treatments and six replicates. The treatments included potato (P1), stale rice (P2), and cassava (P3). Parameters measured were temperature, liquid volume, color, and pH. Results showed that the type of carbohydrate source significantly affected (P < 0.01) temperature, liquid volume, and pH. The highest temperature was observed in the P3 treatment, followed by P2 and P1. The greatest liquid volume occurred in P3, then P2 and P1. Treatment P1 had the highest pH level, followed by P2 and P3. The color of the fermented liquid remained similar to that of the raw materials. The findings indicated that the P3 treatment, involving cassava, produced optimal fermentation results, characterized by low pH levels, suitable temperatures, stable liquid volumes, and color closely resembling the original ingredients.
EVALUASI TINGKAT PENERAPAN BIOSEKURITI PADA PETERNAKAN AYAM BROILER DI KECAMATAN MAIWA KABUPATEN ENREKANG: EVALUATION OF THE LEVEL OF IMPLEMENTATION OF BIOSECURITY IN BROILER CHICKEN FARMING IN MAIWA DISTRICT ENREKANG REGENCY Rahmi, Helmi Rafia; Nugraha, Angga; Purnomo, Nurul
Tropical Animal Science Vol. 7 No. 1 (2025): TROPICAL ANIMAL SCIENCE
Publisher : Universitas Boyolali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36596/tas.v7i1.1844

Abstract

This study aimed to evaluate the level of biosecurity implementation in broiler chicken farms in Maiwa District, Enrekang Regency. Biosecurity is an important component on the livestock health management system that aims to prevent the entry and spread of diseases, and maintain productivity of livestock businesses. The study was conducted descriptively and quantitatively with a survey approach to the entire population of broiler chicken farmers in the area, which amounted to 27 people. Data collection was conducted through direct observation, through direct observation, interviews, and questionnaires. The aspects studied include three main components of biosecurity: traffic control, sanitation, and isolation. The results of this study showed that the implementation of biosecurity by farmers was generally in the sufficient category. The sanitation aspect was the most dominantly implemented, marked by waste management, which had been carried out very well by all respondents (100%). The isolation aspect also showed quite good implementation, with most farmers having separated sick and dead chickens and isolation cages. However, the implementation of biosecurity in the aspect of traffic control is still not optimal, Particularly in relation to guest entry procedures and vehicle disinfectant spraying. Farmers’ characteristics such as education level, farming experience, and gender are known to influence variations in biosecurity implementation. This study is expected to provide a real picture of the field and be a consideration for farmers, the government, and related parties in improving the implementation of biosecurity as a whole.
Polymorphisms of IGF-1 Gene in Indonesian Local Goat Reared Under Smallholder Farmers in Sulawesi Region Rahim, Lellah; Dagong, Muhammad Ihsan Andi; Bugiwati, Sri Rachma Aprilita; Purnomo, Nurul
Jurnal Agripet Vol 24, No 1 (2024): Volume 24, No. 1, April 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i1.28830

Abstract

ABSTRACT. The objective of this study was to determine the allele frequency of IGF-1 in Kacang and Peranakan Ettawa goats in Indonesia. The DNA samples were extracted from the blood of 105 heads of goats and collected from the South Sulawesi and West Sulawesi provinces. The IGF-1 target was amplified using the PCR-RFLP method. Two allele variants (A and B) and three genotypes of the IGF-1 gene (AA, AB, and BB) have been identified in the local goat population. Allele frequencies of IGF-1 were A (0.81) and B (0.19) in the total population of local goats. Allele A is the most common allele in both the Kacang and Peranakan Ettawa goat populations, with the highest frequency found in the Kacang population (0.87). The most common genotype is genotype AA, with the highest frequency in the Kacang population (0.75). The observed and expected heterozygosity were 0.276 and 0.303, respectively. The population of local goats in these regions was in Hardy-Weinberg equilibrium. The conclusion of this research is that the IGF-1 gene in local goats in the Sulawesi region is polymorphic and this diversity information can be used for association studies with growth traits, litter size, and twinning rate.(Polimorpisme gen IGF-1 pada populasi kambing lokal Indonesia yang dipelihara oleh peternak kecil di daerah Sulawesi)ABSTRAK. Penelitian ini bertujuan untuk mengetahui frekuensi alel IGF-1 pada kambing Kacang dan Peranakan Ettawa di Indonesia. Sampel DNA diambil dari darah 105 ekor kambing dan dikumpulkan dari Provinsi Sulawesi Selatan dan Sulawesi Barat. Target IGF-1 diamplifikasi menggunakan metode PCR-RFLP. Dua varian alel (A dan B) dan tiga genotipe gen IGF-1 (AA, AB, dan BB) telah diidentifikasi pada populasi kambing lokal. Frekuensi alel IGF-1 adalah A (0,81) dan B (0,19) pada total populasi kambing lokal. Alel A merupakan alel yang paling banyak ditemukan baik pada populasi kambing Kacang maupun kambing Peranakan Ettawa, dengan frekuensi tertinggi terdapat pada populasi Kacang (0,87). Genotipe yang paling umum adalah genotipe AA, dengan frekuensi tertinggi pada populasi Kacang (0,75). Heterozigositas yang diamati dan diharapkan adalah 0,276 dan 0,303, masing-masing. Populasi kambing lokal di wilayah ini berada dalam keseimbangan Hardy-Weinberg. Kesimpulan yang dihasilkan adalah bahwa gen IGF-1 pada kambing lokal yang dipelihara oleh peternak di wilayah Sulawesi bersifat polimorfik dan informasi keragaman ini dapat digunakan untuk studi asosiasi dengan sifat pertumbuhan, litter size dan kelahiran kembar.