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All Journal Folia Medica Indonesiana Journal of Health (JoH) Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Analis Kesehatan Jurnal Kesehatan Medical Technology and Public Health Journal Medica Hospitalia Medicra (Journal of Medical Laboratory Science/Technology) Academia Open Humanism : Jurnal Pengabdian Masyarakat Medical Scope Journal (MSJ) Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Procedia of Social Sciences and Humanities Journal of Noncommunicable Diseases (JOND) Prosiding University Research Colloquium Indonesian Journal Health Science and Medicine

Andika Aliviameita

Universitas Muhammadiyah Sidoarjo

Author-ID : 2504573

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients Andika Aliviameita; Meryn Linanda Agustin; Anggie Wedha Puspita; Miftahul Mushlih; Puspitasari Puspitasari; Yanik Purwanti; Arief Wisaksono
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.

The Effects of Hyperbaric Oxygen Therapy on Blood Glucose Levels and Lipid Profile in Diabetes Mellitus Patients Andika Aliviameita; Nevy Elensya; Puspitasari Puspitasari; Sri Mukhodim Faridah Hanum
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diabetes mellitus and its complications are still a major health problem in the world. Diabetes mellitus is closely related to the lipid profile, especially concerning the cardiovascular disease. A method that can be used to treat diabetes mellitus is hyperbaric oxygen therapy (HBOT). This study aims to observe changes in blood glucose levels and lipid profiles in patients with diabetes mellitus with hyperbaric oxygen therapy. The determination of blood glucose levels and lipid profiles is done by photometry. A total of 30 patients with diabetes mellitus examined for their blood glucose level and lipid profile before and after hyperbaric oxygen therapy. In the Paired T-test, there were significant decrease in blood glucose levels (p= 0,000), total cholesterol (p= 0,000), and triglycerides (p= 0,000) after hyperbaric oxygen therapy. The conclusion of this study shows there is a significant decrease in blood glucose level and lipid profile (total cholesterol, and triglycerides) after hyperbaric oxygen therapy.

Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte Puspitasari Puspitasari; Evi Rinata; Rohman Dijaya; Siska Aprilia; Dina Trikumalasari; Livia Nur Azzah; Qilmia Fanani; Miftahul Mushlih; Andika Aliviameita; Dian Delta
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p

Korelasi Profil Darah Dengan CRP Serum pada Pasien Diabetes Mellitus dengan Ulkus Diabetikum Andika Aliviameita; Puspitasari Puspitasari; Yanik Purwanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7242

Diabetes mellitus is a metabolic disorder that experiences hyperglycemia and causes various complications, including diabetic foot ulcers (DFU). Hyperglycemia can cause chronic inflammation and decreased function of immune cells. This study aims to determine the correlation between blood profiles and serum C-Reactive Protein (CRP) in diabetes mellitus patients with diabetic ulcers. A total of 30 blood samples of diabetes mellitus patients with blood tests and serum CRP. Blood profile examination used an automatic method with a hematology analyzer, erythrocyte sedimentation rate (ESR) using the Westergreen method, and serum CRP using a semi-quantitative method. In the correlation regression multiple test knowed that the parameters associated with CRP serum are hemoglobin (r = 23.553; p = 0.015), hematocrit (r = -8.678; p = 0.016), leukocyte count (r = 2.098; p = 0.004), and platelet count (r = -0.112; p = 0.002) because it has significance (p < 0.05). Meanwhile, the parameters of erythrocyte count (r = -8.717; p = 0.359), and ESR (r = -0.142; p = 0.109) had no effect on serum CRP levels because they had significance (p> 0.05). The conclusion of this study shows there is a strong correlation (r = 0.777) between blood profile and serum CRP in diabetes mellitus patients with diabetic foot ulcers.

KORELASI ANTARA PROFIL HEMATOLOGI DENGAN PROCALCITONIN PADA PASIEN TERKONFIRMASI COVID-19 puspitasari Puspitasari; Andika Aliviameita; Evi Rinata; Dira Nadila
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7290

Covid-19 is an infectious disease caused by SARS-CoV 2 and has spread very quickly. Several laboratory examinations were carried out on Covid-19 patients including molecular examinations, hematological profiles, and serology. This study aims to determine the relationship between leucocyte, d-dimer, hemoglobin and thrombocyte against procalcitonin in patients with Covid-19. This was an observational analytic study with cross sectional design. A sample of 50 was taken selectively at Aisyiyah Siti Fatimah Tulangan Hospital in November 2020 - January 2021. Multiple linear regression test results showed relationship between leucocyte with procalcitonin (b = -0.64; p = 0.426), d-dimer with procalcitonin (b = 2.702; p = 0.010), hemoglobin with procalcitonin (b = 0.656; p = 0.255), and thrombocyte with procalcitonin (b = 0.037; p = 0.002) . Based on the results, it is found that there is relationship between leucocyte, d-dimer, hemoglobin, and thrombocyte againts procalcitonin in Covid-19 patients.

Pengabdian Masyarakat Penerapan PHBS di Sekolah Yanik Purwanti; Arief Wisaksono; Andika Aliviameita
Aksiologiya: Jurnal Pengabdian Kepada Masyarakat Vol 4, No 2 (2020): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/aks.v4i2.2721

ABSTRAKPerilaku Hidup Bersih dan Sehat sangat dibutuhkan pada siswa di sekolah agar terhindar dari berbagai penyakit. Perilaku Hidup Bersih dan Sehat salah satunya adalah Cuci Tangan dengan sabun dan air bersih, memilih jajanan yang sehat di sekolah, untuk mendukung hal tersebut perlu kerjasama yang baik antara pihak sekolah,orang tua juga siswa dan lingkungan sekitar sekolah. TK dan MI Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo berada di lingkungan pedesaan pingiran Desa, dekat dengan perkebunan sayur, sehingga siswa berpeluang besar untuk melaksanakan perilaku hidup bersih dan jajan sehat di sekolah tapi kenyataannya justru terbalik jajan di luar kantin sekolah, dengan resiko jajanan tidak terkontrol sekolah dan bisa mengancam kesehatan anak. Belum tersedianya tempat cuci tangan dan penyediaan air bersih di sekolah. Jajanan sehat bagi anak perlu diperkenalkan pada siswa juga wali murid sehingga ada kesinambungan kontrol yang baik antara pihak seolah dan wali murid. Maka , perlu diadakan Komunikasi, Edukasi dan Penyuluhan baik kepada siswa maupun wali murid. Dalam wadah parenting yang diadakan oleh TK dan MI Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo Sidoarjo bekerjasama dengan Universitas Muhammadiyah Sidoarjo. Komunikasi, Edukasi dan Penyuluhan yang diadakan pihak sekolah bekerjasama dengan Universitas Muhammadiyah Sidoarjo diharapkan terwujud perilaku Hidup Bersih dan sehat bagi siswa/siswi TK dan MI Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo dan kesadaran bagi siswa dalam menerapkan hidup Bersih dan Sehat (Cuci tangan paka sabun, pengunaan air bersih, jajanan sehat) sehingga terbentuk generasi sehat dan cerdas.Kata Kunci: perilaku bersih; sehat Community Service Implementation of PHBS in SchoolsABSTRACTClean and healthy behavior is needed for students in school to avoid various diseases. Clean and Healthy Behavior one of which is Hand Washing with soap and clean water, choosing healthy snacks at school, to support this it requires good cooperation between the school, parents as well as students and the environment around the school. Kindergarten and MI Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo are located in a rural area near the edge of the village, close to a vegetable plantation, so students have a great opportunity to practice clean living and healthy snacks at school but the reality is actually reversed snacks outside the school canteen, with the risk of snacks. uncontrolled school and can threaten children's health. The lack of a place to wash hands and provide clean water in schools. Healthy snacks for children need to be introduced to students as well as guardians of students so that there is continuity of good control between parties as well as guardians of students. So, it is necessary to hold Communication, Education and Counseling both to students and guardians of students. In the parenting container held by TK and MI Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo Sidoarjo in collaboration with Muhammadiyah University, Sidoarjo. Communication, Education and Counseling held by the school in collaboration with the University of Muhammadiyah Sidoarjo are expected to manifest Clean and healthy life behaviors for kindergarten and MI students Assasul Huda, Kepuh Kemiri Tulangan, Sidoarjo and awareness for students in implementing Clean and Healthy life soap, the use of clean water, healthy snacks) to form a healthy and smart generation.Keywords: clean; healthy behavior

Forensic Identification of Blood Types in Pear (Pyrus bretschneideri) Fruit Bitemark Andika Aliviameita; Mieke Sylvia MAR; Ahmad Yudianto
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Blood type in saliva can be examined through bitemarks on an object left at the crime scene in a crime case as a screening test of suspected perpetrators. Saliva deposited at the bite contains glycoproteins which can carry blood type ABH antigens expressed in the salivary glands and excreted in saliva. Salivary examination is influenced by various external factors that damage saliva, one of which is the duration of exposure to room temperature in a certain period of time. This study aimed to determine differences in protein levels and blood group agglutination titers in (Pyrus bretschneideri) pear bitemark saliva during room temperature exposure within 30, 60 and 90 minutes. This type of study was experimental laboratories with time series design. The samples used were 18 pear bitemarks from 6 individuals who had blood groups A, B and AB then incubated for 30, 60 and 90 minutes. Analysis of protein content using trizol reagent was determined spectrophotometrically, while blood group examination used inhibition absorption method. Anova test showed significant difference between groups of 30 minutes exposure duration with 60 minutes and 90 minutes exposure group (P<0.05; 95% confidence interval). The Pearson correlation obtained the results of -0.739, indicating correlation that the longer exposure to room temperature, the lower the protein content. The results showed that there was a decrease in salivary protein levels and blood group agglutination titers. Salivary protein levels were still detected in the time range of 30, 60 and 90 minutes, so that blood type can still be examined from saliva in bitemarks on pear fruit.

Relation Between Leukocyte Count and CRP (C-Reactive Protein) Levels in Typhoid Fever Patients Alda Megawati; Andika Aliviameita
Academia Open Vol 4 (2021): June
Publisher : Universitas Muhammadiyah Sidoarjo

Typhoid fever is a systemic infectious disease characterized by the patient experiencing fever and abdominal pain due to the spread of Salmonella bacteria. Typhoid fever sufferers cause the immune system to decline which can affect changes in the number of white blood cells and in an infection, an inflammatory process occurs that produces cytokines which are the core stimulators of acute phase protein production, including C-reactive protein (C-reactive protein = CRP). This study was conducted in April 2021 with the aim of knowing the relationship between the number of leukocytes and CRP levels in typhoid fever patients at RA Basoeni Mojokerto Hospital. The design carried out in this study was a laboratory experimental, the sample needed in this study was 30 typhoid fever patients at RA Basoeni Hospital. Examination of the leukocyte count uses an automatic method with a Hematology analyzer, while CRP examination uses a semi-quantitative method. In the Spearman correlation test, it was found that there was no significant relationship between the number of leukocytes and the level of CRP p = 0.460.

Pelatihan Dokter Kecil Sebagai Upaya Mengembangkan Usaha Kesehatan Sekolah di Sekolah Dasar Kabupaten Sidoarjo Andika Aliviameita; Yanik Purwanti; Arief Wisaksono
Jurnal Pemberdayaan Masyarakat Vol 4 No 1 (2019): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

The introduction of a healthy way of life must be done early, and can be started from family and school life. Health care for school-age children can be done through Usaha Kesehatan Sekolah (UKS). The UKS program can be used for coaching in the health sector that requires training of little doctors. The purpose of this activity is to provide training on basic medical materials to improve healthy and healthy living skills, the health status of students, and create a healthy environment so as to increase optimal growth and development. Obtained from the results of this training a positive influence on improving the ability of little doctors. Providing little doctor training provides learning that results in changes from the beginning. This activity involved 42 little doctors from two elementary schools in Sidoarjo. The level of understanding was good, the little doctors rose from (10 students) 23,8% to 69,1% (29 students), quite good understanding from 47,6% (20 students) to 21,4% (9 students), and of the 28,6% (12 students) it dropped to 9,5% (4 students) after receiving a small doctor training.

Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis puspitasari Puspitasari; Andika Aliviameita
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.12667

Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts.

Co-Authors Ahmad Shobrun Jamil Ahmad Yudianto Ainutajriani, Ainutajriani Alda Megawati Amilya, Widya Intan Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Ariyanti, Silvia Asri, Nur Indah Nila Aziza, Vidayatul Batis, Afifah Diah Ariana Dian Delta Dina Trikumalasari Dira Nadila Ellies Tunjung Sari Maulidiyanti, Ellies Tunjung Sari Emilia, Hilda Evi Rinata Fadilatin, Lisa Anis Firmansyah, Nukayo Fitrian Desi Prameswari Hadie, Djauharoh A Intantri, Risma Jamilatur Rohmah Krisdianti, Rika Lestari, Dzulia Livia Nur Azzah Meryn Linanda Agustin Mieke Sylvia Miftahul Mushlih Millah, Hamidah Wardatul Murosidah, Pratasya Liyaajul Mushlih , Miftahul Nevy Elensya NURUL AZIZAH p Puspitasari, p Primastuti, Yuridistya Putri Puspita, Anggun Puspitasari Puspitasari Puspitasari Puspitasari Qilmia Fanani Ramadhan, Syahrul Ibnu Mubaroq Riantika, Mayang Rini, Chylen S. Rini, Chylen Setyo Rohman Dijaya Saidah, Sukma Nur Salim, Siti Zaenab Savitra, Igfina El Segara, Bayu Setiyo Rini, Chylen Silvia Ariyanti Siska Aprilia Siti Zaenab Salim Sri Mukhodim Faridah Hanum Wahyuni, Nining Xena, Alfanny Juliannindy Yanik Purwanti Yasmin, Rizka Afifah Yonika Zakaria, Rizal

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