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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Dinamika Maritim Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Food ScienTech Journal
Agoes Mardiono Jacoeb
Departemen Teknologi Hasil Perairan Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor Jln. Lingkar Akademik, Kampus IPB Dramaga-Bogor 16680 Telp. +622518622915, Fax. +622518622916
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Library & Information Science Other

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PHENOL COMPONENT OF FRESH AND BOILED SEA GRAPES (CAULERPA SP.) FROM TUAL, MALUKU Nurjanah Nurjanah; Agoes Mardiono Jacoeb; Diah Asih Asmara; Taufik Hidayat
Food ScienTech Journal Vol 1, No 1 (2019)
Publisher : University of Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.578 KB) | DOI: 10.33512/fsj.v1i1.6244

Abstract

Caulerpa sp. commonly found in Tual, Southeast Maluku waters. Caulerpa sp. is generally consumed by coastal communities in it is raw form as salads or boiled. Nutrient information and total phenol compounds of Caulerpa sp. during boiling has not been reported. The aim of this study is to determine bioactive components and the levels of total phenol compounds Caulerpa sp. as a result of the boiling process. The boiling process is carried out for 5 minutes at 90 . Boiling process causes loss of flavonoids in Caulerpa sp. Total phenol compounds after boiling decreased by 11.76 mg GAE/g extract. Total phenolic of fresh Caulerpa sp. has better results than by boiling process.
Study of Actinotrichia fragilis Indonesian Red Seaweed as Raw Material for Healthy Salt Nurjanah Nurjanah; Agoes Mardiono Jacoeb; Asadatun Abdullah; Joko Ahadi Priyanto; Naufal Muharam Nurdin; Anggrei Viona Seulalae
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): May 2023
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.753

Abstract

Healthy seaweed salt is low sodium salt from seaweed that offers health benefits for hypertension patients. Indonesian seaweed has the potential to produce healthy seaweed salt. Research to date still focuses on green and brown seaweed but there is still no report for red seaweed. Actinotrichia fragilis is one of red seaweed species that has been discovered in Indonesia’s seawater and has not yet been utilized. Thus, this study aimed to determine the chemical composition and antioxidant activity of A. fragilis flour and the optimum ratio for producing seaweed salt with a high yield, optimum %NaCl, Na/K ratio, and antioxidant activity. Seaweed salt production treatment was the ratio of seaweed flour and distilled water 1:3, 1:5, and 1:10 (w/v), extracted at 40°C for 10 minutes. The mixture was filtered, then dried at 60°C for 30 hours. Data analysis was performed by analysis of variance. The raw material for dried A. fragilis seaweed has a high ash and low-lipid content. Then the ethanol extract had a total phenolic content value of 84.34 mg GAE/g and an antioxidant activity value of 98.22 mg/L. Furthermore, the antioxidant capacity of the ethanol extract was 60.15 mmol ascorbic acid/g and 552.21 mmol Fe2+/g. The best treatment for producing A. fragilis salt is 1:10 with yield of 12.76±0.13%, %NaCl 47.22±1.38%, Na/K ratio 3.32±0.18, IC50 with DPPH and ABTS method 113 mg/L and 87.27 mg/L, total antioxidant capacity 38.21 mg/mL ascorbic acid/g, and 304.32 mmol Fe2+/g. Furthermore, A. fragilis can be used for the production of  healthy seaweed salt.
Komposisi Kimia Tepung dan Aktivitas Antioksidan Ekstrak Ulva lactuca dan Genjer (Limnocharis flava) Sebagai Bahan Baku Pembuatan Garam Rumput Laut Nurjanah Nurjanah; Ramlan Ramlan; Agoes Mardiono Jacoeb; Anggrei Viona Seulalae
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): Juni 2023
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v18i1.931

Abstract

Rumput laut hijau Ulva sp. dapat dimanfaatkan sebagai bahan baku pembuatan garam rendah natrium tinggi kalium dari rumput laut. Peningkatan kandungan kalium dari garam rumput laut dapat ditambahkan dengan bahan alami salah satunya tanaman genjer (Limnocharis flava). Tujuan penelitian ini adalah mendapatkan komposisi kimia tepung dan aktivitas antioksidan ekstrak rumput laut Ulva lactuca dan L. flava sebagai bahan baku pembuatan garam rumput laut. Penelitian terdiri dari beberapa tahapan meliputi preparasi bahan baku U. lactuca dan tanaman genjer, pembuatan tepung rumput laut dan genjer, dan ekstraksi tepung rumput laut dan genjer. Parameter yang dianalisis meliputi komposisi kimia, logam berat, mineral, fitokimia, dan aktivitas antioksidan. Hasil penelitian menunjukkan komposisi kimia tertinggi pada tepung U. lactuca dan L. flava yaitu karbohidrat dengan nilai sebesar 43,55±0,25% dan 53,11±0,23%. Komposisi kimia terendah pada kadar abu tidak larut asam sebesar 0,62±0,02% dan 0,54±0,01%. U. lactuca memiliki komposisi mineral Na 1,61 mg/g, K 18,95 mg/g, dan rasio mineral Na:K 0,08. L. flava memiliki komposisi mineral Na 1,09 mg/g, K 24,67 mg/g, dan rasio mineral Na:K 0,04. Ekstrak U. lactuca memiliki aktivitas antioksidan IC50sangat kuat (48,64±0,65 ppm), kapasitas antioksidan 246,92 µmol asam askorbat/g ekstrak, serta terdeteksi mengandung senyawa alkaloid, flavonoid, fenol hidrokuinon, tanin, dan streoid. Ekstrak L. flava mengandung aktivitas antioksidan IC50 sedang (138,86±0,40 ppm), kapasitas antioksidan 116,92 µmol asam askorbat/g ekstrak, serta terdeteksi mengandung senyawa alkaloid, flavonoid, fenol hidrokuinon, dan steroid. Rumput laut U. lactuca dan tanaman genjer (L. flava) berpotensi digunakan sebagai bahan baku pembuatan garam rendah natrium dari rumput laut. AbstractGreen seaweed Ulva sp. has potential as raw material for production low sodium high potassium salt from seaweed. The potassium content can be increased by adding natural material such as genjer (Limnocharis flava). The aimed of this study was to get the chemical composition of flour and antioxidant activity of extract U. lactuca seaweed and genjer (L. flava) as raw materials for production seaweed salt. The research consisted of several stages including preparation of raw materials, production of seaweed and genjer flour, and extraction of U. lactuca and genjer flour. The result showed that U. lactuca and L. flava have high carbohydrate (43.55±0.25% and 53.11±0.23%) and low acid soluble ash (0.62±0.02% and 0.54±0.01%). U. lactuca has a mineral composition of Na 1.61 mg/g, K 18.95 mg/g, and the ratio of minerals Na:K 0.08. L. flava has a mineral composition of Na 1.09 mg/g, K 24.67 mg/g, and the ratio of minerals Na:K 0.04. U. lactuca extract had very strong IC50 antioxidant activity (48.64±0.65 ppm), antioxidant capacity of 246.92 µmol ascorbic acid/g extract, and was detected to contain alkaloids, flavonoids, phenol hydroquinones, tannins and steroids. L. flava extract contained moderate IC50 antioxidant activity (138.86±0.40 ppm), antioxidant capacity of 116.92 µmol ascorbic acid/g extract, and was detected to contain alkaloids, flavonoids, phenol hydroquinones, and steroids. Seaweed U. lactuca and genjer (L. flava) have potential to be used as raw materials for production low sodium salt from seaweed salt.
Potensi ulvan dari Ulva lactuca sebagai sumber antioksidan: Potential of ulvan from Ulva lactuca as an antioxidant source Agoes Mardiono Jacoeb; Asadatun Abdullah; Siti Nur Hakimah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 27 No 3 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i3.46950

Abstract

Dinding sel Ulva lactuca menghasilkan ulvan yang merupakan hidrokoloid bersulfat dan berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan pengaruh lama pemanasan dalam autoklaf (15 dan 20 menit; 121℃) terhadap rendemen, kadar gula dan sulfat, serta aktivitas antioksidan ulvan. Rendemen ulvan, kandungan gula dan sulfat diukur masing-masing dengan metode gravimetri dan spektrofotometri. Ekstrak kering oven diuji gugus fungsi dan antioksidannya menggunakan FTIR dan metode DPPH. Ulvan hanya bisa dihasilkan melalui ekstraksi pada suhu 121℃ waktu 15 menit. Ekstrak kering ulvan memberikan rendemen 1,98%, kandungan gula total 46,06±0,16% dan kadar sulfat 21,53±1,16%. Spektrum FTIR menunjukkan adanya bilangan gelombang 850 dan 790 cm-1 yang merupakan ciri ulvan. Uji DPPH menghasilkan IC50 sebesar 469 ppm, yang tergolong sebagai antioksidan lemah.
Profil Nutrisi, Mineral dan Kandungan Logam Berat Rumput Laut Cokelat Sargassum sp. Astriani, Aisyah; Nurjanah, Nurjanah; Jacoeb, Agoes Mardiono
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.24274

Abstract

Sargassum sp. is a type of brown seaweed that is quite abundant and grows naturally in coastal waters of Indonesia which is rich in nutritional components such as protein, vitamins, minerals and others that have many benefits (in the food industry, health and beauty). The purpose of this study was to determine the amount of nutrients, minerals and heavy metals contained in Sargassum sp. seaweed obtained from the waters of Ekas Beach, Lombok, Indonesia. The results of proximate analysis of Sargassum sp. seaweed flour showed that protein was the highest result of 43.73%, with fat content with the lowest result of 0.558%. Mineral analysis results showed that potassium (K) had the highest proportion of 27.043 mg/kg and calcium (Ca) had the lowest proportion of 11.936 mg/kg. The resulting Na:K ratio in seaweed flour is 0.853, which meets the WHO standard of no more than one. The results of heavy metal analysis show that heavy metal lead (Pb) 3.596 mg/kg and cadmium (Cd) 0.297 mg/kg have exceeded the maximum limit set by the National Standardization Agency in 2015. This can be caused by the many activities of fishing boats and settlements in the sampling waters. The analysis of acid insoluble ash showed 1.858 mg/kg, which is quite high. Acid insoluble ash content refers to the residual metals or minerals that cannot be dissolved in acid solvents and ash is also a parameter that indicates the cleanliness of the process that can determine food quality.  Sargassum sp. merupakan salah satu jenis rumput laut berwarna coklat yang cukup melimpah dan tumbuh secara alami di perairan pantai Indonesia yang kaya akan komponen nutrisi seperti protein, vitamin, mineral dan lainnya yang memiliki banyak manfaat (dalam industri makanan, kesehatan dan kecantikan). Tujuan dari penelitian ini adalah untuk mengetahui jumlah nutrisi, mineral dan logam berat yang terkandung dalam rumput laut Sargassum sp. yang diperoleh dari perairan Pantai Ekas, Lombok, Indonesia. Hasil analisis proksimat tepung rumput laut Sargassum sp. menunjukkan bahwa protein merupakan hasil tertinggi yaitu 43,73%, dengan kadar lemak dengan hasil terendah yaitu 0,558%. Hasil analisis mineral menunjukkan bahwa kalium (K) memiliki proporsi tertinggi yaitu 27,043 mg/kg dan kalsium (Ca) memiliki proporsi terendah yaitu 11,936 mg/kg. Rasio Na:K yang dihasilkan pada tepung rumput laut adalah 0,853, yang memenuhi standar WHO yaitu tidak lebih dari satu. Hasil analisis logam berat menunjukkan bahwa logam berat timbal (Pb) 3,596 mg/kg dan kadmium (Cd) 0,297 mg/kg telah melebihi batas maksimum yang ditetapkan oleh Badan Standarisasi Nasional pada tahun 2015. Hal ini dapat disebabkan oleh banyaknya aktivitas kapal nelayan dan pemukiman di perairan pengambilan sampel. Hasil analisis abu tidak larut asam menunjukkan angka 1,858 mg/kg, cukup tinggi. Kadar abu tidak larut asam mengacu pada sisa logam atau mineral yang tidak dapat larut dalam pelarut asam dan abu juga merupakan parameter yang mengindikasikan kebersihan proses yang dapat menentukan kualitas pangan. 
Optimization of Genetic Material Extraction Techniques and Application of Isothermal Amplification Method for Field Authentication of Two Thresher Sharks (Alopias pelagicus and Alopias superciliosus) Aldina, Vatia Dwi Rizki Ramadhani; Abdullah, Asadatun; Rianti, Puji; Jacoeb, Agoes Mardiono
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.147-154

Abstract

The pelagic thresher shark (Alopias pelagicus) and bigeye thresher shark (Alopias superciliosus) are important shark species for Indonesia's consumption and finning industry. Both Alopias species are included in the CITES appendix II, thus requiring certain documents for trading. Regarding species identification for on-site application, the DNA isolation method is a crucial step. In this study, we developed a DNA isolation method suitable for on-site application based on isothermal amplification (LAMP) and species-specific COI gene markers. Three different extraction methods were applied, namely modified spin column kits and dipsticks. The quality of DNA was evaluated and tested for isothermal amplification using a reference sample, fresh fillet, and ethanol-preserved sample. The extracted sample concentration was in the range of 135.35-0.65 ng/μL. The LAMP test showed that three different DNA extraction methods successfully amplified the DNA fragments through the color changes at the end point of the LAMP reaction. The LAMP test was also sufficient to detect less than 10 ng of DNA from A. pelagicus and A. superciliosus within 30-50 min. The DNA from the modified spin column and dipstick extraction method combined with LAMP can potentially be used to detect Alopias pelagicus and Alopias superciliosus species on-site.
PENYIMPANAN MINYAK IKAN TUNA (Thunnus sp.) DENGAN PENAMBAHAN ANTIOKSIDAN DALAM KEMASAN BOTOL KACA Hanifah, Nur Inda Annisa'ul; Suseno, Sugeng Heri; Jacoeb, Agoes Mardiono
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 20, No 2 (2024): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.20.2.91-100

Abstract

Ikan Tuna merupakan jenis pelagis besar, industry pengolahan ikan tuna menghasilkan produk samping, salah satunya minyak ikan. Minyak ikan kaya akan PUFA (EPA dan DHA). Penelitian ini bertujuan untuk mengetahui profil asam lemak, kandungan logam berat, kejernihan, dan nilai oksidasi minyak ikan tuna mentah, menentukan campuran minyak ikan tuna murni dengan penambahan nilai antioksidan dan mengetahui pengaruh pengemasan botol kaca selama penyimpanan. Penelitian ini dibagi menjadi dua tahap. Tahap pertama adalah karakterisasi minyak ikan tuna mentah dan pemurnian menggunakan metode degumming dan bleaching. Tahap kedua adalah penambahan antioksidan alfa-tokoferol (0,4%; 0,6%; 0,8%), minyak zaitun (5%, 15%, 25%, 35%, 45%), minyak dedak padi (5%, 15 %, 25%, 35%, 45%), kemudian diuji menggunakan metode real-time pada suhu kamar. Perlakuan terbaik pada minyak ikan tuna tahap kedua disimpan dalam berbagai botol kaca dengan metode schaal oven test selama 12 hari. Hasil kandungan asam lemak tertinggi pada minyak ikan tuna mentah, minyak zaitun, dan minyak dedak padi berturut-turut adalah DHA, asam oleat, dan asam oleat. Residu logam berat merkuri memenuhi standar SNI, tingkat kejernihan lebih dari 70%, dan nilai parameter oksidasi memenuhi standar IFOS. Perlakuan terbaik minyak ikan tuna murni selama penyimpanan menggunakan metode real-time pada suhu kamar yang memenuhi standar IFOS adalah campuran 0,6% α -tokoferol. Perlakuan terbaik minyak ikan dalam kemasan botol kaca dengan metode schaal oven test yang memenuhi standar IFOS adalah botol kaca warna coklat.  
Co-Authors - - nurjanah - - Nurjanah Agus Oman Sudrajat Ahmad Khoirudin Rizkon Aldina, Vatia Dwi Rizki Ramadhani Anggrei Viona Seulalae Anhar Rozi Aninta - Saraswati Ari Elisa Ratih Asadatun Abdullah Astriani, Aisyah Aulia Azka Aulia Sekar Sari Bagja Adhitia Putera Desie Rachmania Detti - Pujianti Diah Asih Asmara Dudu Abdulatip Faiza A Dali Galih Wendi Pradana Ginanjar Pratama Ginanjar Pratama Hanani Putri Yocinta Hanifah, Nur Inda Annisa'ul isna kurniati afifudin Joko Ahadi Priyanto Kamini Kamini Lenny Asnita Br Lingga Luh Putu Ratna Sundari Mala Nurilmala Maya Sofia Miftakhul Arifin Mohammad Mukhlis Kamal N. Nurjanah N. Nurjanah N. Nurjanah Naufal Muharam Nurdin Nurjanah Nurjanah Nurjanah - - Nurjanah - Nurjanah Nurjanah Hasil Perikanan Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurokhmatunnisa - - Pipih Suptijah Pipin Supinah Puji Rianti Ramlan Ramlan Ramlan Rezki Kamila Rianda Gita Fetrisia RIDWAN AFFANDI Rinto . Rita Marsuci Harmain Riyanda Perdiansyah Rizsa Mustika Pertiwi Rofi Ahmad Dzaky Roni Nugraha Ronny I. Wahju Ruddy Suwandi Rudy Chrystiawan Sabri Sudirman Selvanda Moreine Bunga Shabrina Aulia Siluh Putu Sri Dia siluh putu sri dia utari Siti Nur Hakimah Sri Ayu Insani Sri Purwaningsih Stefanus Senoadi Kristiono Sugeng Heri Suseno Tatag Budiardi TATI NURHAYATI Taufik Hidayat Taufik Hidayat Taufik Hidayat Taufik Hidayat Uut Tri Utami Vickar Muhammad Widi Sulistiono Widyana Ayu Kristantina Yanti Sinaga Yuliana Syalviana Fatuni Yuniarti, Tatty Zahidah mawardi