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Journal : METAMORFOSA Journal of Biological Sciences

STUDI FENOLOGI PERBUNGAAN Lilium longiflorum Thunb. Ni Wayan Deswiniyanti; Ida Ayu Astarini; Ni Made Puspawati
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Lily (Lilium longiflorum Thunb.) was known as ornamental plants, especially as cut flower. Lily varieties in Indonesia are still lacking and the price is quite expensive but demand of lily is increased steadily. The first step for successful plant breeding is understanding phenophysiology of the plants, in particular flowering phenology. The aim of this research is to provide information on flowering lily phenology, to be a basic knowledge in improving for planning plant breeding programs. In this research, plants were observed from bud initiation until fruit production (phase F0-F4). Additional morphologies observed such as: high plant, leaf shape and color, shape and type of root or bulb. Result shows that period of phase F0-F3 of sampled lily is 52 days. The plant height of lily is about 50-150 cm, has elongated single lancet leaf shape, green leaf, bulbs, and has fiberous root.
OPTIMALISASI MEDIA ORGANIK UNTUK PERBANYAKAN ANGGREK HITAM (Coelogyne pandurata Lindl.) SECARA IN VITRO Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p13

Abstract

Populasi anggrek hitam (Coeloegyne pandurata Lindl.) terancam punah karena akibat eksploitasi yang berlebihan. Masalah tersebut dapat diatasi dengan teknik perbanyakan anggrek melalui kultur in vitro, namun harga media kultur jaringan relatif mahal. Tujuan penelitian ini adalah untuk memperoleh informasi tentang respon pertumbuhan benih anggrek hitam pada media kultur bahan organik dan modifikasinya, sehingga dapat digunakan sebagai media alternatif untuk pertumbuhan anggrek hitam yang optimal. Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan 3 perlakuan yaitu media organik 1 (kentang), 2 (pisang ambon) dan 3(ubi jalar) masing-masing 10 ulangan. Data dianalisis secara statistik menggunakan Analisis Varians dan Uji Jarak Berganda Duncan pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa media organik kentang dan pisang ambon memiliki waktu tumbuh paling cepat dan jumlah tunas paling banyak dibandingkan media organik ubi jalar. Secara statistik kontaminasi tidak dipengaruhi secara signifikan oleh media, jadi dalam hal ini media paling baik/optimum adalah kentang dan pisang ambon, yang berbeda nyata dengan ubi jalar pada waktu tumbuh dan jumlah tunas.
PERSILANGAN INTERSPESIFIK ANGGREK HITAM (Coelogyne pandurata) DENGAN ANGGREK MUTIARA (Coelogyne asperata) Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p15

Abstract

Black orchid (Coelogyne pandurata) and Pearl orchid (Coelogyne asperata) are endemic orchid from Kalimantan forest, shape and color suitable as ornamental flower. Many people collected the orchids from the wild for commercial purpose but not much effort on their propagation. To prevent population decreased, propagation and production of new varities via interspesific hybridisation need to be done. An important step required in plant breeding is to obtain cross compatiblity. Cross compatibility of some orchids from Indonesia are not known, including compatibility of Coelogyne pandurata x Coelogyne asperata. Information on compatibility and fertility is very important to produce good quality seed as propagation material. In this research pollination was performed on orchid plants at 2 until 5 day after flowering and pollination was done in the morning. There are three replicates for each pollination.  Pollination was done with C. pandurata (black orchid) as male parent and C. asperata (pearl orchid) as female parent. The results showed that successful pollination was obtained from pollination at 2 days after flowering. Seed capsule was harvested at 3 months after pollination but not yet perfectly ripe. Seed germination employed in vitro method on Vacin & Went (VW) and VW with modification media. Seed from C. pandurata x C. asperata abled to germinate on VW media with addition of 0,1 mg/l auxin and 0.1 mg/l cytokinin and germinated 9 weeks after planting.