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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Jurnal Agroekoteknologi Buletin Kebun Raya BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science JURNAL BUDIDAYA PERTANIAN Berkala Penelitian Agronomi Buletin Palma Jurnal Sains Agro Indonesian Journal of Applied Research (IJAR) International Journal of Agriculture System
Diny Dinarti
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Journal : Journal of Tropical Crop Science

 1 
In Vitro Mutagenesis of Etlingera elatior by Gamma Ray Intermittent Irradiation Azzahra, Elmi Irmayanti; Aisyah, Syarifah Iis; Dinarti, Diny; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.45 KB) | DOI: 10.29244/jtcs.5.3.111-118

Abstract

Etlingera elatior is tropical ornamental plant commonly called “torch ginger” from Zingiberaceae family. Conventional breeding of E. elatior is limited by cross incompatibility, poor fruit set and low seed production.  In this study irradiation of E. elatior with Gamma ray performed to induce mutation. This study was aimed to increase morphological diversity and to obtain unique morphological characters to increase the aesthetic value of E. elatior as ornamental plants and cut flower. Two genotypes of E. elatior, red and white flowers, were tested. The LD20, LD35, and LD50 were determined following intermittent (split dose) Gamma irradiation with a two-hour gap between each gamma ray shot. Red genotype E. elatior explants were irradiated with dose of 3 + 3 Gy (LD20); 4 + 4 Gy (LD35) and 5 + 5 Gy (LD50); white genotype were irradiated with a dose of 2 + 2 Gy (LD20); 2.8 + 2.8 Gy (LD35) and 3.7 + 3.7 Gy (LD50). Non-irradiated explants were set as control.  The results of this study indicated that the increase in dose of gamma ray irradiation changed shoot length and number of leaves in the red genotype per explant as compared to control. Morphological changes occurred in leaf shape at 5 + 5 Gy and 3.7 + 3.7 Gy and formation of variegated leaves at 2.8 + 2.8 Gy and 5 + 5 Gy. Keywords: mutation breeding, ornamental plant, split irradiation dose, torch ginger, Zingiberaceae
Increasing Ploidy Level of Garlic (Allium sativum L.) “Tawangmangu Baru” In-Vitro Using Colchicine Hailu, Molla Gebreyohannes; Wiendi, Ni Made Armini; Dinarti, Diny
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.122-136

Abstract

“Tawangmangu Baru” garlic variety is known to have low productivity. The variety is still highly demanded due to its strong flavour and aroma; however, its production has not yet been able to fulfill the local needs of Central Java due to the small size and limited production area. This study aimed to determine the effect of concentration and time duration of colchicine treatment towards increasing the ploidy level of “Tawangmangu Baru” garlic variety for genetic variability. The experimental design used in this study was a complete randomized design with two factorials and 12 combinations. The first factor was concentration of colchicine, i.e. 0.00, 0.02, 0.04, 0.06, 0.08 and 0.10%, and the second factor was the immersion time, i.e. 24 and 48 hours. The result indicated that, 4.72% callus induction was obtained in BDS + 0.4 mg.L-1 2,4-D + 2.0 mg.L-1 kinetin; and 4.0% callus proliferation were obtained in both BDS + 1.5 mg.L-1 2,4-D + 1.0 mg.L-1 kinetin and MS +1.5 mg.L-1 2,4-D and 1.0 mg.L-1 kinetin. The untreated plantlets showed higher mortality rate compared to the explants with 48 hours colchicine treatment. Higher number of shoots were recorded in 0.1% colchicine at 48 hours and lower shoots in 24 hours, whereas 0.1% colchicine at 24 and 48 hours showed the highest ploidy level of total nuclear DNA analyzed by flow cytometry. The genetic diversity of the “Tawangmangu Baru” garlic was successfully enhanced by colchicine and immersion treatment. Mutant lines with tetraploid and mixoploid plants were obtained. The putative lines obtained at 0.1% colchicine treatment were subcultured to produce new mutants before testing the phenotype. The application of colchicine at 24 and 48 hours treatment improved the genetic potential of “Tawangmangu Baru” garlic variety in vitro. The application of colchicine increased the ploidy level and an increase in ploidy is expected to make the bulb size larger. Larger tuber size will increase the tuber weight, and also the overall garlic productivity and production in the future.
Co-Authors , Krisantini Agus Purwito Ali Husni Anas Dinurrohman Susila Aqlima , Aqlima, nFN Arfan Nazhri Simamora Arfan Nazhri Simamora ARSYAD, MIRZA ARSIATY ASMONO, DWI Awang Maharijaya Aziz, Sandra Arifin Azzahra, Elmi Irmayanti Bambang S. Purwoko Cartealy, Imam Civi Cartealy, Imam Civi Chitra Priatna Cucun Yuliana Debi Rani Mutiara Dendih Sukmadijaya Devit Purwoko Dodo R. Sastra Donny Hartanto Dwi Murti Puspitaningtyas, Dwi Murti Efendi, Darda Efi Toding Tondok Erviana Eka Pratiwi Fahmi Muhammad Cokrosudibyo Fathur Rahmi, Atika Fatsan, Ahmad Fitri Fatma Wardani Fitri Fatma Wardani Fitri Rachmawati Fitri Yulianti Fitro Adi Cahyo Hailu, Molla Gebreyohannes Halimah Widyaningrum Imam Civi Cartealy Ismail Maskromo Isna Tustiyani Joko Ridho Witono Khairiyah, Hayat Kusumawardani, Annisa Try Marai Rahmawati Maria Utami Dewi Wardhani, Maria Utami Dewi Megayani Sri Rahayu Meliyana Mufa'adi, Ardianto Muhamad Syukur Muhammad Alif Baharudin Muhammad Reza Zakie Nazhira, Amalia Ni Made Armini Wiendi Nurul Khumaida Nurul Khumaida Prabawati Hyunita Putri Purwoko, Bambang Sapto Purwoko, Devit purwoko, devit Rachmani, Inda Hidayati Rahmadara, Gemilang Rahmi Fajri Refa Yulianingsih Rusmiyati, Henny Ryan Budi Setiawan Sandra A. Aziz Sandra Arifin Aziz Sari Nurulita Sari, Dewi Citra Sari, Rahmah Dian Shandra Amarillis Siti Shofiya Nasution Sobir Sobir SRI HENDRASTUTI HIDAYAT Sri Wening Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO, nFn Sugiyanta Suparjo Syaiful Anwar Syarifah Iis Aisyah Teuku Tajuddin Teuku Tajuddin TINCHE, nFn Ulil Azmi Nurlaili Afifah Urip Sayekti Wening Kusuma Wardani Widaningsih, Ida Wika Anrya Darma, Wika Anrya Willy Bayuardi Suwarno Winarni, Utin Winarso D. Widodo Witono, dan Joko Ridho Yayu Alitalia Yuliana, Cucun Yupi Isnaini Yusnita Sari, Yusnita Yusup Bahrul Ulum