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All Journal Jurnal Veteriner Jurnal Teknologi Laboratorium Journal of Health (JoH) Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Journal of Health Sciences Medical Technology and Public Health Journal Medical Laboratory Analysis and Sciences Journal Jurnal Riset Biologi dan Aplikasinya Medicra (Journal of Medical Laboratory Science/Technology) Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Jurnal Pengabdian Masyarakat dalam Kesehatan Humanism : Jurnal Pengabdian Masyarakat Jurnal Abdimas Kartika Wijayakusuma Indonesian Journal of Innovation Studies Manajemen Pelayanan Kesehatan Indonesian Journal Health Science and Medicine

Miftahul Mushlih

Universitas Muhammadiyah Sidoarjo

Author-ID : 2542712

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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PHBS SANTRI DAN APLIKASI UKS PONDOK PESANTREN AL-HAMDANIYAH BUDURAN SIWALAN PANJI SIDAORJO Nurul Azizah; Jamilatur Rohmah; Miftahul Mushlih; Paramitha Amelia Kusumawardani
Jurnal Pengabdian Masyarakat Dalam Kesehatan Vol. 2 No. 1 (2020): APRIL 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpmk.v2i1.19583

Pendahuluan: Berdasarkan survey di Pondok pesantren Al-Hamdaniyah kami menemukan santri yang sakit terbaring dikamar hanya beralaskan kain dan selimut. Kondisi ini sangat tidak nyaman karena 1 kamar berisi 10 anak dengan berbagai macam aktivitas yang dapat menimbulkan kegaduhan sehingga dapat mempengaruhi istirahat santri yang sakit. Selain itu dapat dibilang berbahaya karena penyakit kemungkinan dapat menularkan pada santri di sekelilingnya. Di pondok tidak ada UKS dan kader kesehatan sehingga yang sakit kadang tidak terurus. Santri pondok pesantren Al-Hamdaniyah tidak pernah mendapatkan pendidikan tentang pola hidup bersih dan sehat di lingkungan pondok pesantren.Metode: Kegiatan pengabdian masyarakat dilakukan pada tanggal 11 Maret 2020 dilaksanaan workshop dan penyuluhan tentang perilaku hidup bersih dan sehat baik secara individu maupun di lingkungan pondok pesantren, kemudian dilanjutkan dengan bimbingan serta pembentukan kader kesehatan, kemudian dilanjutkan dengan pengadaan UKS (Usaha Kesehatan Sekolah) yang akan dimanfaatkan bagi santri yang sedang sakit untuk istirahat atau sekedar cek kesehatan dan melakukan perawatan sakit ringan. Beberapa santri juga akan kami bentuk untuk menjadi kader kesehatan, agar dapat mengenali tanda gejala awal adanya kesakitan sebelum di periksakan kepada tenaga medis, dan dapat melakukan perawatan mandiri setelah santri berobat hingga santri dapat sembuh dengan baikHasil: kegitan seminar PHBS di lingkungan pondok pesantren berlangsung lancar, dan para santri terlihat antusias mengikuti acara hingga selesai. Pembinaan kader kesehatan telah dilakukan, dan para calon kader kesehatan dapat mengkuti pembinaan dengan baik. Tersedianya UKS baru yang dapat menjadi fasilitas tambahan dan bermanfaat bagi santri.Kesimpulan: Program pengabdian masyarakat ini telah dilaksanakan. Dan menghasilkan sebuah perubahan sesuai dengan target luaran, yakni pengetahuan tentang PHBS individu dan lingkungan pondok pesantren, terbentunya kader kesehatan untuk menjalankan kegiatan UKS, terwujudnya UKS di pondok pesantren Al-Hamdaniyah.

PERBANDINGAN IDENTIFIKASI Toxoplasma gondii MENGGUNAKAN METODE POLYMERASE CHAIN REACTION (PCR) DAN METODE ENZYME LINKED FLUORESCENT ASSAY (ELFA) Miftahul Mushlih; Alifia Nurfitriana; Kurnia Wahyu Ningsih; Nurul Azizah; Nia Lukita Arian
Meditory : The Journal of Medical Laboratory Vol 8, No 2 (2020): Meditory, Volume 8, No 2, Tahun 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v8i2.1266

Toxoplasmosis is an infectious disease that does not show specific symptoms. This study aimed to determine the differences results of T. gondii detection in human blood using the PCR (polymerase chain reaction) with the ELFA (Enzym Linked Fluorescent Assay) method. The research was done by descriptive exploratory methods. Samples taken from 9 West Sumatra Pramita Clinics Laboratory and 1 person came from Sidoarjo as a control. Data analyzes was conducted by a chi-square test with a 95% confidence level. In the ELFA method detects IgG Anti-Toxoplasma. Whereas the molecular test detects B1 gene target. The serological test was able to detect 9 patients of toxoplasmosis and 1 patient was negative/ control. The molecular test showed a specific band with 195 bp in length. The accuracy of the molecular test was 77% (T count (4.4) T table (3.84). Based on the results its can be concluded that there a difference in identification between the PCR method and the ELFA method.

Comparison of PCR (Polymerase Chain Reaction) Results Using DNA Template Isolation Results with Column and Resin Methods: Perbandingan Hasil PCR (Polymerase Chain Reaction) Menggunakan DNA Template Hasil Isolasi dengan Metode Column dan Resin Dinda Apriliana; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

DNA isolation is a basic technique in molecular biology where DNA is separated from various unnecessary components. There are various methods of DNA isolation but the most commonly used methods are column and resin methods. The purpose of this study was to compare the results of PCR using DNA template isolated by column and resin methods. The research method used in this research is descriptive experimental using 8 whole blood samples. Samples were tested quantitatively with UV-VIS spectrophotometer and qualitatively using PCR. This research was conducted from February to April 2021. The results showed that based on quantitative DNA testing, DNA samples isolated using the column method had higher purity than the resin isolation method, which means that the resin method contained a lot of contamination. However, in terms of concentration, the resin method had a higher concentration than the column method and there was no significant difference in the purity and concentration of DNA if the sample was isolated using the column method and the resin method (r=0.169, r=0.252) after the dependent T test was performed. . In the analysis using PCR, samples isolated by the column method showed a fairly clear band compared to the resin method.

Identification of Genes in Positive Alleles Marking Type 2 Diabetes Mellitus Using Primer A18: Identifikasi Gen pada Alel Positif Penanda Diabetes Melitus Tipe 2 Menggunakan Primer A18 Aminatul Firda; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Diabetes mellitus type 2 (DMT2) is a disorder characterized by hyperglycemia due to impaired insulin secretion and insulin resistance. The purpose of this study was to identify the gene involved in the positive allele of DMT2 marker using A18 primer and is a type of exploratory descriptive study. The samples used were 2 samples of DMT2 patients from the villages of Taman and Balongbend, and 8 samples of DNA collection at the Molecular Biology Laboratory, Muhammadiyah University of Sidoarj. Based on the genetic analysis carried out, the GATB gene (Glutamyl-tRNA Amidotransferase Sub Unit B) was identified in the positive allele with a homology value of "percentage identity" between the sample sequence and the sequence data in the NCBI Genbank of 80 and 88%. This research hypothesizes that the GATB gene has a relationship with the emergence of DMT2 as evidenced by the relationship between the gene and the performance of mitochondria to produce ATP which is needed by pancreatic beta cells to produce insulin.

Identification of Genes in Positive Alleles Marking Type II Diabetes Mellitus Using Primer D20: Identifikasi Gen pada Alel Positif Penanda Diabetes Melitus Tipe II Menggunakan Primer D20 Erina Wahyu Kartikasari; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Diabetes Mellitus is a metabolic disorder disease characterized by an increase in blood glucose due to insulin resistance (impaired insulin function) and or a decrease in insulin secretion. Genetically, Type 2 Diabetes Mellitus is influenced by genes that affect glucose metabolism in the body. The purpose of this study was to identify the genes involved in type 2 diabetes mellitus patients who were amplified using D20 primers. The method used in this research is descriptive experimental using 2 whole blood samples and 8 samples collected from DNA isolation in the Molecular Biology Laboratory of Type 2 Diabetes Mellitus patients. The results of the research based on the BLAST program obtained matched gene sequences from the sequenced leucine rich repeats gene. and guanylate kinase domain containing (LRGUK). The LRGUK gene is thought to be associated with Type 2 Diabetes Mellitus because the presence of SNP (Single Nucleotide Polymorphism) in the area around the LRGUK gene (7q32) shows a significant relationship with fasting blood glucose.

Comparison of the Quality of DNA Template Isolation Results of the Resin Method with and Without Centrifugation: Perbandingan Kualitas DNA Template Hasil Isolasi Metode Resin dengan dan Tanpa Sentrifugasi Liah Dwi Jayanti; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

The centrifugation process is able to separate the blood into several components, including the buffy coat. Buffy coat contains white blood cells that have a cell nucleus which is the source of DNA. This research uses descriptive experimental method. The samples used were 16 venous blood samples consisting of 8 samples without centrifugation (wholeblood) and 8 samples centrifuged (buffycoat). DNA isolation using the resin method. Quantitative analysis was performed using a UV-Vis spectrometer. The results showed that the average concentration and purity of DNA in the centrifuged sample was higher than in the sample without centrifugation. The result of this research is that the centrifuged sample (buffycoat) can be used for the DNA isolation process and has a higher purity and concentration value than the sample without centrifugation (wholeblood).

Comparison the Quality of Template DNA isolated by Column Method with and without Centrifugation: Perbandingan Kualitas DNA Template yang diisolasi dengan Metode Kolom dengan dan tanpa Sentrifugasi Aji Cakra Wardana; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

DNA isolation is the first step in molecular biology work. The quality of the DNA template is very important because it can affect the DNA amplification reaction. The application of centrifugation to the sample is expected to produce a Buffy coat layer containing nuclear cells. This study aims to compare the quality of the DNA template isolated from the column with and without centrifugation. This research used descriptive experimental method. The samples used were 16 samples with the division of 8 samples given centrifugation treatment and 8 samples without centrifugation. DNA isolation column method is a DNA isolation method that uses a silica membrane, where the silica membrane works as a filter for DNA that is split from the cell. The DNA isolation method column was carried out according to the standard protocol on the Geneaid DNA mini Kit Blood/Tissue. The concentration and purity of DNA were measured using UV-Vis Spectrophotometry at 260 nm and 280 nm. Visualization of DNA isolation results was carried out using 1% agarose gel. The results of measuring the quality of DNA samples using the column method with centrifugation showed better results with clearer visualization of DNA bands than samples without centrifugation. The results of the T dependent statistical test on both samples of p value < 0.05 showed that there was a significant difference in DNA quality with centrifuged and non-centrifuged treatment.

Differences in DNA Purity Test Using UV-Vis Spectrophotometer and Nanodrop Spectrophotometer in Type 2 Diabetes Mellitus Patients: Perbedaan Uji Kemurnian DNA Menggunakan Spektrofotometer UV-Vis dan Spektrofotometer Nanodrop pada Pasien Diabetes Melitus Tipe 2 Pandu Aji Dewanata; Miftahul Mushlih
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

DNA quality test is one of the basic techniques in Molecular Biology which aims to determine the presence or absence of protein and RNA contamination. DNA quantity test can be done using a spectrophotometer. Spectrophotometer is a tool used to measure the concentration of a compound based on its ability to absorb light. The purpose of this study was to determine differences in DNA purity as measured by UV-Vis Spectrophotometer and Nanodrop Spectrophotometer. The method used in this research is descriptive exploratory with purposive sampling technique. The number of samples used is 15 samples. The results of this study are that there are differences in the concentration and purity of DNA from quantitative tests with UV-Vis spectrophotometers and Nanodrops obtained based on statistical tests using the T Dependent Test. The result of this research is that in measuring DNA purity there is a difference in DNA purity because the results of Sig (2-Tailed) use 0.001 or below 0.05 (Ha is accepted). And based on statistical tests on DNA concentration measurements, it is known that there are differences in DNA concentrations.

POTENTIAL MOLECULES AGAINST COVID-19 FROM ANNONA MURICATA; AN IN-SILICO APPROACH Miftahul Mushlih; Andika Aliviameita; Puspitasari Puspitasari; Ahmad Shobrun Jamil
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 6 No 1 (2022): Medical Technology and Public Health Journal March 2022
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v6i1.3069

At the end of 2019, a new virus emerged from Wuhan, China named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The key to viral infection to host cells is the use of the ACE2 receptor. This study aimed to explore the potential of drugs in Annona muricata plant using the Insilico method. Compounds obtained from KNApSAcK Based on the analysis using SwissADME (Lipinski creteria)criteria. 22 compounds passed the selection. Eight ligands were identified as being able to change the conformation of the initial form of RBD and ACE2 attachment. Eight molecules are able to change the initial conformation, namely Asimilobine, Coreximine, (+)-Stepharine, Coclaurine, Norcorydine, N- Nornuciferinhe, methoxyphenyl)methyl]-1,2,3,4-tetrahydroisoquinolin-5-ol, and Atherosperminine.

Pengenalan Pemeriksaan Berbasis Nucleic Acid Amplification Tests (NAATs) Di Sekolah Kesehatan Miftahul Mushlih; Puspitasari Puspitasari; Andika Aliviameyta
Darmabakti : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 4 No 2 (2023): Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Lembaga Peneliian dan Pengabdian Masyarakat (LPPM) Universitas Islam Madura (UIM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/darmabakti.2023.4.2.153-159

Perkembangan pemeriksaan terbaru banyak mengarah dengan memanfaatkan kode unik dan spesifik yang ada di DNA. Pemeriksaan ini dikenal luas dengan nukleic acid amplification tests (NAATs). Kesenjangan pendidikan antara perkembangan teknologi dengan penerapan di sekolah memunculkan suatu masalah tersendiri. Tujuan dari kegiatan ini untuk melakukan pengenalan dan workshop secara langsung metode NAATs oleh Prodi teknologi laboratorium medis (TLM), UMSIDA kepada SMK Mitra Sehat Sidoarjo. Kegiatan dilakukan melalui tiga tahap, yaitu penjajakan untuk mengetahui tingkat implementasi pengenalan NAATs di sekolah, Pengenalan dasar teori pemeriksaan, dan Workshop secara langsung, yaitu siswa diajak untuk melakukan praktikum secara langsung pada kasus identifikasi Toxoplasma gondii. Selain dengan melatih keahlian siswa, kemampuan siswa juga diukur melalui pretest dan posttest. Berdasarkan analisis yang dilakukan terdapat peningkatan pengetahuan dan kemampuan siswa dalam mengenal dan melakukan pemeriksaan NAATs.

Co-Authors Ahmad Shobrun Jamil Aini, Aqiliyanti Nur Aji Cakra Wardana Alifia Nurfitriana Amin, Hindah Sabrina Aminatul Firda Andika Aliviameita Andika Aliviameyta Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Aulia, Faradila Nur Bayu Segara Budi Setiadi Daryono Chylen Setiyo Rini Dian Delta Dina Trikumalasari Dinda Apriliana Djauharoh Djauharoh Erina Wahyu Kartikasari Evi Rinata Fitri Kumala Sari Fitrian Desi Prameswari Hadie, Djauharoh A Hindah Sabrina Amin Iknan, Siti Asriani Imamah, Siti Aliyatul Jamilatur Rohmah Kurnia Wahyu Ningsih Liah Dwi Jayanti Livia Nur Azzah Liyaajul, Pratasyah Meryn Linanda Agustin Nia Lukita Arian NUR EFENDI NURUL AZIZAH Nurul Azizah Pandu Aji Dewanata Paramitha Amelia Kusumawardani Puspitasari Puspitasari Puspitasari Puspitasari Puspitasari Qilmia Fanani Rohman Dijaya Sari, Azizah Krismonita Sari, Fitri Kumala Segara, Bayu Siska Aprilia Siti Asriani Iknan Siti Cholifah Tis’iyyah , Siti Nur Maghfiroh Yanik Purwanti Zakaria, Rizal

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