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Optimasi Kecepatan Sentrifugasi Sampel Darah untuk Pemeriksaan Diabetes Melitus Tipe 2 (T2D) Berbasis Molekuler Miftahul Mushlih; Fitrian Desi Prameswari; Jamilatur Rohmah; Andika Aliviameita; Mushlih, Miftahul
Journal of Health Vol. 12 No. 2 (2025): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v12n2.5

Type 2 Diabetes Mellitus (T2D) is a metabolic disorder caused by dysfunction of insulin secretion by pancreatic beta cells and the inability of insulin tissue to respond appropriately to insulin. Molecular-based examination can make it easier to determine appropriate diagnostic biomarkers and the biology of this disease appears long before clinical symptoms develop. Blood isolation samples from the buffy coat contained higher levels of DNA than whole blood samples. The aim of this research is to determine whether there are differences in the results of the centrifugation speed of blood samples for molecular-based examination of Type 2 Diabetes Mellitus (T2D). Optimal centrifugation speed can produce high-quality DNA required for molecular analysis, thereby improving the accuracy of diagnosis and effectiveness of T2D treatment. DNA contaminated by cellular debris can result in small amounts of DNA obtained for further analysis. The research was carried out qualitatively using descriptive experimental research using purposive sampling techniques. The total samples used were 25 samples, taken from 5 patients and then divided into 5 treatment groups (no centrifugation, centrifugation speed 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes). Test testing is carried out qualitatively (electrophoresis). The results showed that there was no significant difference in DNA visualization in molecular examination of T2D disease between blood samples that were not centrifuged and those that were centrifuged at speeds of 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes. However, centrifugation speeds between 1500 rpm and 4500 rpm showed thicker and clearer DNA bands.

Optimasi Kecepatan Sentrifugasi Sampel Darah untuk Pemeriksaan Diabetes Melitus Tipe 2 (T2D) Berbasis Molekuler Miftahul Mushlih; Fitrian Desi Prameswari; Jamilatur Rohmah; Andika Aliviameita; Mushlih, Miftahul
Journal of Health Vol. 12 No. 2 (2025): Journal of Health (JoH) - July
Publisher : LPPM STIKES Guna Bangsa Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v12n2.5

Type 2 Diabetes Mellitus (T2D) is a metabolic disorder caused by dysfunction of insulin secretion by pancreatic beta cells and the inability of insulin tissue to respond appropriately to insulin. Molecular-based examination can make it easier to determine appropriate diagnostic biomarkers and the biology of this disease appears long before clinical symptoms develop. Blood isolation samples from the buffy coat contained higher levels of DNA than whole blood samples. The aim of this research is to determine whether there are differences in the results of the centrifugation speed of blood samples for molecular-based examination of Type 2 Diabetes Mellitus (T2D). Optimal centrifugation speed can produce high-quality DNA required for molecular analysis, thereby improving the accuracy of diagnosis and effectiveness of T2D treatment. DNA contaminated by cellular debris can result in small amounts of DNA obtained for further analysis. The research was carried out qualitatively using descriptive experimental research using purposive sampling techniques. The total samples used were 25 samples, taken from 5 patients and then divided into 5 treatment groups (no centrifugation, centrifugation speed 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes). Test testing is carried out qualitatively (electrophoresis). The results showed that there was no significant difference in DNA visualization in molecular examination of T2D disease between blood samples that were not centrifuged and those that were centrifuged at speeds of 500 rpm, 1500 rpm, 3000 rpm and 4500 rpm for 5 minutes. However, centrifugation speeds between 1500 rpm and 4500 rpm showed thicker and clearer DNA bands.

Dermatophyte Fungi Causing Tinea Unguium in Toenails of Builders in Bangkalan: Jamur Dermatophyte Penyebab Tinea Unguium pada Kuku Jari Kaki Pekerja Konstruksi di Bangkalan Aini, Aqiliyanti Nur; Rini, Chylen Setiyo; Mushlih, Miftahul; Puspitasari
Indonesian Journal on Health Science and Medicine Vol. 2 No. 2 (2025): Oktober
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v2i2.219

General Background: Tinea unguium is a nail plate infection commonly caused by dermatophyte fungi, frequently affecting individuals exposed to moist and contaminated environments. Specific Background: Builders are one of the occupational groups at high risk due to their constant contact with damp, dirty, and unhygienic surroundings. Knowledge Gap: Despite its prevalence, limited studies have focused on identifying specific dermatophyte species responsible for toenail infections among construction workers in Bangkalan, Madura. Aims: This study aimed to identify dermatophyte fungi causing Tinea unguium in the toenails of builders in Bangkalan. Results: Using a descriptive cross-sectional design with 28 purposively selected nail samples, findings revealed infections in 18 participants by Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermatophyton floccosum, while 10 samples showed non-dermatophyte fungi (Aspergillus sp. and Scopulariopsis). Novelty: This research provides the first documentation of both dermatophyte and non-dermatophyte fungi associated with toenail infections among builders in this region. Implications: The findings underscore the need for occupational health awareness and preventive strategies targeting fungal nail infections among construction workers. Highlights: First identification of toenail dermatophytes in builders of Bangkalan. Found both dermatophyte and non-dermatophyte fungi species. Supports occupational health awareness for fungal infections. Keywords: Tinea Unguium, Dermatophyta, Toenails, Builders, Bangkalan

Body Mass Index and Cholesterol and Uric Acid Levels in Diabetes: Indeks Massa Tubuh dan Kadar Kolesterol serta Asam Urat pada Penderita Diabetes Aulia, Faradila Nur; Mushlih, Miftahul
Indonesian Journal on Health Science and Medicine Vol. 2 No. 2 (2025): Oktober
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v2i2.221

General Background: Diabetes mellitus (DM) is a chronic metabolic disorder affecting millions worldwide, often linked to impaired insulin secretion or function. Specific Background: Dyslipidemia and hyperuricemia are frequent metabolic disturbances in DM, and body mass index (BMI) is commonly used to assess nutritional status, yet its role in predicting cholesterol and uric acid levels among DM patients remains unclear. Knowledge Gap: While previous studies suggest possible associations, evidence on whether BMI significantly correlates with cholesterol and uric acid in DM is inconsistent. Aim: This study investigated the relationship between BMI and cholesterol and uric acid levels in DM patients. Results: A cross-sectional study of 30 respondents at Anna Medika Madura General Hospital used CHOD-PAP and Uricase-PAP methods, showing cholesterol levels ranging 136–278 mg/dl, uric acid 3.4–12.1 mg/dl, and BMI 18.0–34.5 kg/m². Statistical analysis (Chi-square, p > 0.05) indicated no significant association between BMI and cholesterol or uric acid. Novelty: This study emphasizes that BMI alone is insufficient to predict lipid and uric acid abnormalities in DM patients. Implications: Findings highlight the need for comprehensive metabolic assessment beyond BMI for effective DM management. Highlights: No significant link between BMI and cholesterol or uric acid in DM. BMI values ranged from normal to obese, but metabolic changes varied. Clinical monitoring of DM requires broader parameters beyond BMI. Keywords: Diabetes Mellitus, Body Mass Index, Cholesterol, Uric Acid, Cross-Sectional Study

Identifikasi Mutasi RS7903146 pada Gen TCF7L2 pada Penderita Diabetes Tipa II di Wilayah Sidoarjo Huesein,; Mushlih, Miftahul; Rini, Chylen Setiyo; Hanum, Galuh Ratmana
Sains dan Matematika Vol. 8 No. 1 (2023): April, Sains & Matematika
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/sainsmat.v8n1.p28-31

Diabetes Mellitus Tipe 2 adalah gangguan sistem endokrin yang dipengaruhi oleh faktor genetik dan pilihan gaya hidup. Mutasi pada gen TCF7L2, khususnya pada rs7903146, telah erat terkait dengan T2DM karena efek gangguan pada sekresi insulin. Tujuan utama dari penelitian ini adalah mengidentifikasi mutasi pada gen TCF7L2 dalam populasi T2DM di Wilayah Sidoarjo. Sebanyak tujuh sampel digunakan dalam penelitian ini dengan mengaplikasikan teknik isolasi DNA, PCR, dan sekuensing. Sampel DNA secara khusus diperbanyak untuk menargetkan gen TCF7L2 selama 318 pasangan basa. Hasil penelitian ini menunjukkan ketiadaan mutasi pada rs7903146. Type 2 Diabetes Mellitus is an endocrine system disorder influenced by genetic factors and lifestyle choices. Mutations in the TCF7L2 gene, particularly at rs7903146, have been closely associated with T2DM due to their disruptive effects on insulin secretion. The primary objective of this study is to identify mutations in the TCF7L2 gene within the T2DM population of the Sidoarjo Region. A total of seven samples were utilized in this research, employing DNA isolation, PCR, and sequencing techniques. The DNA samples were specifically amplified to target the TCF7L2 gene over a stretch of 318 base pairs. The findings of this study indicate an absence of mutations at rs7903146.

Pendampingan Sekolah Dasar Negeri 4 Kupang, Jabon dalam Menghadapi New Normal Mushlih, Miftahul; Segara, Bayu; Hadie, Djauharoh A; Zakaria, Rizal; Aliviameita, Andika
Humanism : Jurnal Pengabdian Masyarakat Vol 1 No 2 (2020): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v1i2.5565

Kejadian pandemi Corona menyebabkan perubahan seluruh aspek kehidupan di masyarakat termasuk di dunia pendidikan. Pemerintah telah memutuskan adanya kehidupan normal yang mengharuskan setiap orang terbiasa untuk hidup berdampingan dengan wabah virus Corona. Tujuan dari kegiatan ini adalah untuk memberikan bimbingan dan dukungan fasilitas di SDN 4 Kupang, Jabon, Sidoarjo dalam rangka menghadapi kehidupan dunia normal (new normal) akibat adanya pandemi covid 19. Metode yang digunakan adalah Pembimbingan dan pelatihan kepada guru dan siswa. Peserta dari kegiatan ini meliputi guru SDN 4 Kupang (6 Orang) dan seluruh siswa SD N dari kelas 1 sampai kelas 6 yang berjumlah 19 orang. Hasil dari kegiatan ini adalah ditemukan kendala pada sekolah guna menghadapi kehidupan baru diantaranya meliputi kesulitan menyediakan fasilitas pendukung wajib new normal serta kesadaran siswa yang masih rendah untuk berkegiatan sesuai dengan norma kehidupan baru. Luaran dari kegiatan ini adalah terselesaikannya kendala sekolah di dalam menghadapi kehidupan yang normal dan meningkatnya wawasan guru dan siswa wabah covid 19.

Comparison of PCR (Polymerase Chain Reaction) Results Using DNA Template Isolation Results with Column and Resin Methods: Perbandingan Hasil PCR (Polymerase Chain Reaction) Menggunakan DNA Template Hasil Isolasi dengan Metode Column dan Resin Apriliana, Dinda; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

DNA isolation is a basic technique in molecular biology where DNA is separated from various unnecessary components. There are various methods of DNA isolation but the most commonly used methods are column and resin methods. The purpose of this study was to compare the results of PCR using DNA template isolated by column and resin methods. The research method used in this research is descriptive experimental using 8 whole blood samples. Samples were tested quantitatively with UV-VIS spectrophotometer and qualitatively using PCR. This research was conducted from February to April 2021. The results showed that based on quantitative DNA testing, DNA samples isolated using the column method had higher purity than the resin isolation method, which means that the resin method contained a lot of contamination. However, in terms of concentration, the resin method had a higher concentration than the column method and there was no significant difference in the purity and concentration of DNA if the sample was isolated using the column method and the resin method (r=0.169, r=0.252) after the dependent T test was performed. . In the analysis using PCR, samples isolated by the column method showed a fairly clear band compared to the resin method.

Identification of Genes in Positive Alleles Marking Type 2 Diabetes Mellitus Using Primer A18: Identifikasi Gen pada Alel Positif Penanda Diabetes Melitus Tipe 2 Menggunakan Primer A18 Firda, Aminatul; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Diabetes mellitus type 2 (DMT2) is a disorder characterized by hyperglycemia due to impaired insulin secretion and insulin resistance. The purpose of this study was to identify the gene involved in the positive allele of DMT2 marker using A18 primer and is a type of exploratory descriptive study. The samples used were 2 samples of DMT2 patients from the villages of Taman and Balongbend, and 8 samples of DNA collection at the Molecular Biology Laboratory, Muhammadiyah University of Sidoarj. Based on the genetic analysis carried out, the GATB gene (Glutamyl-tRNA Amidotransferase Sub Unit B) was identified in the positive allele with a homology value of "percentage identity" between the sample sequence and the sequence data in the NCBI Genbank of 80 and 88%. This research hypothesizes that the GATB gene has a relationship with the emergence of DMT2 as evidenced by the relationship between the gene and the performance of mitochondria to produce ATP which is needed by pancreatic beta cells to produce insulin.

Identification of Genes in Positive Alleles Marking Type II Diabetes Mellitus Using Primer D20: Identifikasi Gen pada Alel Positif Penanda Diabetes Melitus Tipe II Menggunakan Primer D20 Kartikasari, Erina Wahyu; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

Diabetes Mellitus is a metabolic disorder disease characterized by an increase in blood glucose due to insulin resistance (impaired insulin function) and or a decrease in insulin secretion. Genetically, Type 2 Diabetes Mellitus is influenced by genes that affect glucose metabolism in the body. The purpose of this study was to identify the genes involved in type 2 diabetes mellitus patients who were amplified using D20 primers. The method used in this research is descriptive experimental using 2 whole blood samples and 8 samples collected from DNA isolation in the Molecular Biology Laboratory of Type 2 Diabetes Mellitus patients. The results of the research based on the BLAST program obtained matched gene sequences from the sequenced leucine rich repeats gene. and guanylate kinase domain containing (LRGUK). The LRGUK gene is thought to be associated with Type 2 Diabetes Mellitus because the presence of SNP (Single Nucleotide Polymorphism) in the area around the LRGUK gene (7q32) shows a significant relationship with fasting blood glucose.

Comparison of the Quality of DNA Template Isolation Results of the Resin Method with and Without Centrifugation: Perbandingan Kualitas DNA Template Hasil Isolasi Metode Resin dengan dan Tanpa Sentrifugasi Jayanti, Liah Dwi; Mushlih, Miftahul
Indonesian Journal of Innovation Studies Vol. 15 (2021): July
Publisher : Universitas Muhammadiyah Sidoarjo

The centrifugation process is able to separate the blood into several components, including the buffy coat. Buffy coat contains white blood cells that have a cell nucleus which is the source of DNA. This research uses descriptive experimental method. The samples used were 16 venous blood samples consisting of 8 samples without centrifugation (wholeblood) and 8 samples centrifuged (buffycoat). DNA isolation using the resin method. Quantitative analysis was performed using a UV-Vis spectrometer. The results showed that the average concentration and purity of DNA in the centrifuged sample was higher than in the sample without centrifugation. The result of this research is that the centrifuged sample (buffycoat) can be used for the DNA isolation process and has a higher purity and concentration value than the sample without centrifugation (wholeblood).

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