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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Biomedicine and Translational Research Indonesian Journal of Biotechnology Majalah Obat Tradisional The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) ACI (Acta Cardiologia Indonesiana) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) International Journal of Applied Sciences and Smart Technologies JURNAL PENELITIAN KESMASY Indonesian Archives of Biomedical Research (InABR) Indonesian Journal of Biomedicine and Clinical Sciences
Nur Arfian
Departement Of Anatomy, Embryology, And Anthropology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Endothelin-converting enzyme-1 mRNA expression correlates with downregulation of kallikrein and upregulation of endothelin a receptor after ureteral ligation in mice: ECE-1 downregulates Kallikrein expression upon UUO Nur Arfian; Dian Prasetyo Wibisono; Ghassani Rahman; Valerie Hassani Putri; Ardiles Varian; FBA Ummiyah; Eryna Ayu Nugra Desita; Nungki Anggorowati; Dwi Cahyani Ratna Sari
Indonesian Journal of Biomedicine and Clinical Sciences Vol 58 No 4 (2026): in press
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v58i4.33823

Abstract

Endothelin-converting enzyme-1(ECE-1), endothelin A receptor (ETAR) and kallikrein-kinin system (KKS) may play roles in the pathophysiology of kidney disease. ECE-1, an important protein responsible for endothelin-1 (ET-1) production, may contribute to renal fibrosis due to kidney obstruction. However, KKS has shown protective roles in kidney injury. In this study, we investigated the relationship between Kallikrein, ETAR, and ECE-1 mRNA expression in a unilateral ureteral obstruction (UUO) model in mice. Twenty male Swiss Webster mice (age 8-12 weeks, body weight 30-40 grams) were divided into 3 groups: negative control/Sham-operated group (SO, n=6), mice with UUO which were sacrificed on day 7 (D7UUO, n=7), and day 14 (D14UUO, n=7). Tubular injury score was analysed by Periodic Acid Schiff (PAS) staining, and immunohistochemistry of α-SMA was conducted to examine myofibroblast formation. ECE-1, Kallikrein, and ETAR mRNA expressions were quantified using reverse transcriptase-PCR (RT-PCR). UUO induced tubular injury and destruction of renal architecture, with higher scores in the D7UUO and D14UUO groups (p<0.001). Myofibroblast area fraction also revealed a significantly higher area fraction in D7UUO (p<0.001) and D14UUO (p<0.001) compared to SO. Furthermore, UUO promoted ECE-1 upregulation that was associated with Kallikrein downregulation. We also found a significantly upregulated expression of ETAR in the D14UUO group compared to the SO and D7UUO groups. Ureteral ligation may induce upregulation of ECE-1 and ETAR expression and downregulation of Kallikrein expression, leading to their contributions in kidney disease.
Co-Authors Afifah Afifah Amelia Amelia Ardiles Varian Arif Prassetyo Baiq R Rhadianaa Christiana Tri Nuryana Danarto Danarto Danarto Danarto Daniel Agriva Tamba Dian Prasetyo Wibisono Dian Prasetyo Wibisono Didik Setyo Heriyanto Dwi Aris Agung Nugrahaningsih Dwi Cahyani Ratna Sari Dwi Cahyani Ratna Sari Dwina Agrila Lakabela Eko Purnomo Eryna Ayu Nugra Desita Fajar Dwi Astarini Fauziyatul Munawaroh FBA Ummiyah Ferdinandus Bayu Satria Gharini, Putrika Prastuti Ratna Ghassani Rahman Gilang Argya Dyaksa Hana Rutyana Putri Antonio Handoyo Pramusinto Herianto . Herianto Herianto Heru Yulianto Hilma Kholida Ats-tsani I Dewa Putu Pramantara I G.B. Budi Dharma Indwiani Astuti Indwiani Astuti Irwan Supriyanto Juananda, Desby Junaedy Yunus Lina Choridah M. Mansyur Romi Mae Sri Hartati Wahyuningsih Maharsa Pradityatama Masita Muchtar Mawaddah Ar-Rochmah Muhammad Mansyur Romi Ngatidjan Ngatidjan Nondang Purnama Siregar Nungki Anggorowati Nungki Anggorowati Nunu N Madjid Nur Amin Nyoman Kertia Pramita Dwinda Mulyana Pratiwi Indah Sayekti Putri, Rachma Greta Perdana Riky Setyawan Ronny Martien Ronny T Wirasto Santosa Santosa Sari Eka Pratiwi Sofia Mubarika Haryana Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Susini R Sari Tety Hartatik Tiara Meirani Valeria Savista Tiara Puspita Agustin Toni Febriyanto Valerie Hassani Putri Widya Wasityastuti Widya Wasityastuti Wiwit Ananda Wahyu Setyaningsih Yeshua Putra Krisnugraha Yohanes Widodo Wirohadidjojo Yosef Agung Cahyanta