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All Journal JPTK: Jurnal Pendidikan Teknologi dan Kejuruan Neo-Bis INDONESIAN JOURNAL OF APPLIED PHYSICS Jurnal Dinamika Sosial Budaya Jurnal Riset Ekonomi dan Bisnis Menara Perkebunan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ilmiah Edunomika (JIE) SOLUSI Jurnal Tanah dan Iklim Jurnal Bingkai Ekonomi (JBE) Jurnal Pengabdian kepada Masyarakat Indonesia (JPKMI) Jurnal Informasi dan Komunikasi Administrasi (JIKAP) Unisia Sustainable Business Journal
Djoko Santoso
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Kloning parsial gen penyandi enoil-ACP reduktase dari mesokarp buah kelapa sawit (Elaeis guineensis Jacq.) Partial cloning of gene encoding enoyl-ACP reductase from mesocarp of oil palm (Elaeis guineensis Jacq.) Asmini BUDIANI; Djoko SANTOSO; Hajrial ASWIDINNOOR; Antonius SUWANTO
E-Journal Menara Perkebunan Vol 72, No 1: Juni 2004
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.327 KB) | DOI: 10.22302/iribb.jur.mp.v72i1.126

Abstract

Summary Enoyl-ACP reductase (ENR) is a component of fatty acid synthase (FAS) that is considered to play an important role in fatty acid elongation and oil accumulation of several plants. One of the proteins expressed coinciding with fruit development and oil accumulation in oil palm has been detected from the previous study and had homology with ENR. Therefore, as a part of genetic engineering program to improve oil content and quality in oil palm mesocarp, this research was aimed to clone cDNA conserved region of gene encoding enoyl-ACP reductase from oil palm mesocarp. Based on the amino acid sequence of the polypeptide that was homologous with ENR and combined with information of conserved region sequences of the same gene from other plant sources, primers were designed for amplifying conserved region of the ENR gene. Amplifi-cation was carried out by RT-PCR using total RNA as template, at several annealing temperatures and MgCl2 concentrations. Amplification product was cloned using pCR 2.1-TOPO, and the sequence was subjected into BlastN analysis. The results confirmed that the cloned cDNA fragment with 698 bp in size was the conserved region of the ENR gene.  This sequence was highly homologous with the same gene from other plants such as Oryza sativa, Olea europaea, Brassica napus, Triticum aestivum and Arabidopsis thaliana with E-value 1e-96, 7e-77, 2e-64, 5e-41 and 3e-36, respectively. Based on this result, primers have been made and used to amplify the 5’- and 3’ ends of the ENR -cDNA  of oil palm mesocarp. Ringkasan Enoil-ACP reduktase (ENR) merupakan salah satu komponen asam lemak sintase (FAS) yang berperan penting dalam pemanjangan asam lemak dan akumulasi minyak pada berbagai tanaman. Salah satu protein yang ter-ekspresi sejalan dengan tahapan perkembangan buah sawit dan akumulasi minyak pada penelitian sebelumnya diketahui mempunyai homologi dengan ENR. Oleh karena itu, sebagai salah satu bagian dari usaha rekayasa metabolisme minyak pada mesokarp buah sawit, penelitian ini bertujuan untuk mengklon cDNA daerah konservatif gen penyandi ENR dari mesokarp buah sawit. Berdasarkan  sekuen asam amino polipeptida yang mempunyai homologi dengan ENR dan dikombinasikan dengan hasil penjajaran daerah konservatif gen tersebut dari berbagai anaman lain, dirancang primer  untuk  amplifikasi daerah konservatif ENR. Amplifikasi dilakukan dengan RT-PCR menggunakan templat RNA total pada berbagai suhu   penempelan   dan   konsentrasi    MgCl2. Hasil amplifikasi dimurnikan dari gel dan diklon menggunakan vektor kloning pCR2.1-TOPO serta dianalisis nya menggunakan BlastN. Hasilnya mengkonfirmasi fragmen cDNA terklon berukuran 698 pb sebagai daerah konservatif ENR tersebut mempunyai homologi tinggi dengan gen yang sama dari    O. sativa,  O. europaea, B. napus, T. aestivum dan  A. thaliana masing-masing dengan E-value 1e-96, 7e-77, 2e-64, 5e-41 dan 3e-36. Berdasarkan hasil tersebut telah dibuat primer spesifik untuk amplifikasi cDNA daerah ujung 5’- dan 3’- gen  ENR dari mesokarp kelapa 
Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods) Imron Riyadi; Darda EFENDI; Bambang S PURWOKO; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.376 KB) | DOI: 10.22302/iribb.jur.mp.v1i1.258

Abstract

AbstractA right combination of cytokinin is able to support the process of callus differentiation to somatic embryo formation in plant somatic embryogenesis. Liquid culture application could increase the efficiency of in vitro culture process on plants. This research aimed to determine the best concentration of TDZ combined with kinetin for callus differentiation to  somatic embryo of sago palm on three culture methods. Plant material used was embryogenic callus derived from tips meristem culture from sucker of Alitir sago palm. Callus was cultured on modified MS media added with: 0.0, 0.1, 0.5 and 1.0 mg/L TDZ combined with 0.5 mg/L kinetin for 12 weeks with subcultures every 6 weeks. Three culture methods used were suspension, temporary immersion system (TIS), and solid media. There were 12 treatments with 4 replicates. The results showed that the highest number of somatic embryos was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in 6 weeks (167.3 embryos/flask) and 12 weeks (389.2 embryos/flask) with its fresh weight of 18.4 g and 29.1 g, respectively. The highset survival rate in final culture (12 weeks) was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin (100%). The shortest time for somatic embryos expression was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in two weeks after culture. Histological analysis of early-stage somatic embryos showed the presence of dense and compact cellular arrangements which formed growth spot axis for shoot or SAM (shoot apical meristem) and root or RAM (root apical meristem) that connected each other. [Key words: culture method, embryogenic callus, Metroxylon sagu Rottb., kinetin, sago palm, TDZ]   AbstrakAplikasi kombinasi sitokinin yang tepat dapat mendorong proses diferensiasi kalus membentuk embrio somatik pada proses embriogenesis somatik tanaman. Penggunaan metode kultur cair dapat meningkatkan efisiensi proses kultur in vitro tanaman. Penelitian ini bertujuan untuk menentukan konsentrasi TDZ terbaik dikombinasikan dengan kinetin dalam proses diferensiasi kalus membentuk embrio somatik tanaman sagu pada tiga metode kultur. Bahan tanam penelitian  berupa kalus embriogenik tanaman sagu asal kultur meristem pucuk dari anakan sagu jenis Alitir. Kalus dikulturkan pada media modifikasi dengan penambahan  TDZ dengan konsentrasi 0,1; 0,5; dan 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L selama 12 minggu yang disubkultur pada umur 6 minggu. Metode kultur yang digunakan terdiri atas tiga macam yaitu: kultur suspensi, sistem perendaman sesaat (SPS) dan media padat. Perlakuan terdiri atas 12 kombinasi perlakuan dengan empat ulangan. Hasil penelitian menunjukkan bahwa rerata jumlah embrio somatik tertinggi dicapai pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L baik pada umur kultur 6 minggu (167,3 buah) maupun umur 12 minggu (389,2 buah). Rerata bobot segar tertinggi juga diperoleh pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L  pada umur kultur 6 minggu (18,4 g) dan  12 minggu (29,1 g). Rerata daya hidup kultur akhir (12 minggu) tertinggi  sebesar 100% diperoleh pada perlakuan SPS. Induksi embrio somatik  tercepat yakni setelah  dua minggu diperoleh pada  metode kultur SPS dengan TDZ 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L. Analisis histologi embrio somatik stadium awal  menunjukkan adanya susunan sel yang rapat dan kompak yang menyusun semacam poros atau berkas titik tumbuh tunas atau SAM (shoot apical meristem) maupun akar atau RAM (root apical mersitem) yang saling terhubung.[Kata kunci: kalus embriogenik, metode kultur, kinetin, TDZ, sagu, Metroxylon sagu]
Identifikasi homolog TcAGL-15 untuk penanda embriogenesis tanaman kakao melalui pendekatan bioinformatika Identification of TcAGL-15 homolog in the embryogenic culture from the zygotic embryo explant Oktaviany Ferry TRIASTANTO; Muhammad JUSUF; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 74, No 2: Desember 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.908 KB) | DOI: 10.22302/iribb.jur.mp.v74i2.102

Abstract

Summary One of the major problems encountered in micropropagation of cacao through tissue culture is very low frequency of embryo formation. Embryogenesis is believed to have key regulatory gene determining the process. Understanding such gene may help to solve problems in the regeneration process. One of the genes reported to involve in the embryogenesis is AGAMOUS-like 15 (AGL-15). This gene has an important role in the regulation of early embryogenesis in several plants. This experiment aimed to identify AGL-15 homolog in cacao through bioinformatics approach. The first step of this experiment is to identify the AGL-15 homolog using hetero-logous primers from DNA genomic isolated from leaves of cacao plants. The sequence of the AGL-15 fragment was used in designing specific primers for longer AGL-15 fragment. These primers were then used to identify AGL-15 gene using total RNA isolated from cultured zygotic embryos. Differential pattern of AGL-15 gene expression was observed in zygotic embryos cultured for five weeks. AGL-15 heterologous primers designed from several plants could be used to identify cacao AGL-15 homolog. The putative cacao AGL-15 gene could be identified from zygotic embryos. The differential pattern of the AGL-15 gene expression is a strong indication that AGL-15 can be used as an embryogenesis marker in cacao plants.Ringkasan Salah satu kendala perbanyakan kakao melalui kultur jaringan adalah sulitnya embriogenesis, yang diduga melibatkan satu atau lebih gen kunci yang menentukan proses tersebut. Keberhasilan mengidentifikasi gen-gen kunci akan membantu menyelesaikan masalah dalam regenerasi embrio kakao. Salah satu gen yang diduga terlibat dalam proses ini adalah AGAMOUS-like 15 (AGL-15). Gen ini berperan pada regulasi selama masa awal perkembangan embrio beberapa tanaman. Penelitian ini bertujuan untuk mengidentifikasi homolog AGL-15 pada kakao melalui pen-dekatan bioinformatika dan RT-PCR. Pene-litian diawali dengan identifikasi homolog AGL-15 dari DNA genomik daun kakao meng-gunakan primer heterologus. Sekuen fragmen homolog AGL-15 yang diperoleh, kemudian digunakan untuk merancang primer spesifik AGL-15 yang berukuran lebih panjang. Primer ini selanjutnya digunakan untuk meng-identifikasi gen AGL-15 dari RNA total embrio zigotik. Pengamatan pola pita gen AGL-15 dilakukan pada kultur in vitro embrio zigotik yang berumur lima minggu. Primer hetero-logus gen AGL-15 yang berasal dari berbagai tanaman, mampu mengidentifikasi keberadaan homolog  gen  tersebut  pada  tanaman   kakao. Fragmen homolog AGL-15 putatif tanaman kakao teridentifikasi pada tingkat RNA embrio. Dengan adanya pola diferensial dari ekspresi gen AGL-15 hingga lima minggu pertama perkembangan embrio, ada indikasi kuat bahwa fragmen homolog AGL-15 dapat menjadi penanda embriogenesis pada tanaman kakao.
Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions Tetty CHAIDAMSARI; Rita HAYATI; Auzar SYARIEF; Aswaldi ANWAR; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 77, No 2: Desember 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.668 KB) | DOI: 10.22302/iribb.jur.mp.v77i2.179

Abstract

SummaryAttempts to improve productivity of cacaoplantations lead us to study the molecularmechanism of flowering. In the model speciesArabidopsis thaliana as well as some otherspecies, LEAFY is a central regulatory gene forthe transition of shoot apical meristems toflowering meristems. Different from that ofArabidopsis, cacao inflorescence is acauliflorous type, by which flowers can developrepeatedly from the same flower cushion on thetrunk. In this research, a LEAFY homolog wasisolated from active flower cushion with RT-PCRusing a pair of DNA primer specifically designedto isolate its complete cds. Gel electrophoresisexamination indicated the presence of a 1.2 kbamplicon. Purified from the gel, this DNAfragment was cloned into competent cells ofE. coli XL1 Blue using pGEM-T Easy cloningvector at an orientation according to the T7promoter of the plasmid. Sequence analysis usingBLASTX, showed that the amplicon was LEAFY(LFY) homolog. Alignment analysis using ClustalW indicated that the cTcLFY highly homologousto those from other perennial crops such ascitrus, grape, apple and poplar. The highesthomology (conserved region) was found in the Cterminal of the encoded proteins.RingkasanUsaha untuk meningkatkan produktivitasperkebunan kakao telah mendorong penelitianmolekuler tentang mekanisme pembungaankakao. Pada tanaman model Arabidopsis thalianadan lainnya, LEAFY merupakan gen kunci dalamtransisi meristem tunas jadi meristem bunga.Berbeda dengan sistem pada Arabidopsis,pembungaan kakao termasuk tipe cauliflorous,bunga dapat muncul dari bantalan bunga yangsama sepanjang tahun. Dalam penelitian inihomolog LFY diisolasi dari bantalan bunga aktifmenggunakan RT-PCR dengan sepasang primerspesifik yang dirancang berdasarkan sekuenDNA di kedua ujung gen tersebut. Pemeriksaangel elektroforesis menunjukkan adanya amplikontunggal berukuran 1,2 kb. Setelah dimurnikandari gel, amplikon dapat diklon ke dalam selkompeten E. coli galur XL1 Blue menggunakanvektor pGEM-T Easy dengan orientasi yangsesuai dengan promoter T7 dari vektor. AnalisisBLASTX sekuen DNA membuktikan bahwaamplikon tersebut adalah homolog dari genLEAFY. Analisis penjajaran dengan mengguna-kan ClustalW menunjukkan bahwa gen cTcLFYtersebut memiliki homologi yang tinggi dengangen sejenis dari tanaman keras lainnya sepertitanaman jeruk, anggur, apel dan poplar.Homologi tertinggi (daerah terkonservasi)terdapat pada ujung (terminal) C dari proteinyang disandinya.
Karakterisasi gen penyandi lipase dari kapang Rhizopus oryzae dan Absidia corymbifera Characterization of gene encoding lipase from fungus Rhizopus oryzae and Absidia corymbifera Riza A PUTRANTO; Djoko SANTOSO; . TRI-PANJI; . SUHARYANTO; Asmini BUDIANI
E-Journal Menara Perkebunan Vol 74, No 1: Juni 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.091 KB) | DOI: 10.22302/iribb.jur.mp.v74i1.118

Abstract

SummaryLipase is a group of enzymes which catalyze fat hydrolysis. Lipase is recently used to produce diacylglycerol (DAG) from triacylglycerol (TAG). Lipase  can be used to produce healthy oil. Having a rich biodiversity, Indonesia has the opportunity to produce lipase using indigenous microbes, such as molds. This research aimed to detect  LIPASE  gene on several strains of molds employing PCR technique. Genomic DNAs were isolated from four strains of molds (M. sitophila, R. oryzae, R. microsporus, and A. corymbifera). Heterologous primers for LIPASE  were designed based on the conserved region of 12 LIPASE  sequences accessed from GenBank and used to amplify the genomic DNA resulted in a 466 bp fragmen. BLAST analysis showed that the bands of DNAs have high homology with common lipase protein in several strains of  Rhizopus.Ringkasan Lipase merupakan kelompok enzim yang berfungsi sebagai biokatalis hidrolisis lemak. Lipase banyak digunakan untuk konversi triasilgliserol (TAG) menjadi diasilgliserol (DAG). Penggunaan lipase penting untuk produksi minyak sehat (healthy oil). Indonesia dengan keanekaragaman hayati tinggi berpeluang besar   mengembangkan   produksi   lipase   dari mikroba lokal, salah satunya adalah kapang. Deteksi gen merupakan langkah awal dalam upaya peningkatan produksi lipase melalui rekayasa genetika. DNA genomik empat galur kapang (M. sitophila, R. oryzae, R. microsporus, dan A. corymbifera) telah berhasil diisolasi. Sepasang primer heterologous telah berhasil dirancang berdasarkan daerah terkonservasi 12 sekuen gen LIPASE dari GenBank. Amplikon DNA yang diperoleh pada PCR menggunakan pasangan primer RLP memiliki panjang 466 bp. Analisis BLAST memperlihatkan bahwa amplikon PCR memiliki homologi yang tinggi dengan protein LIPASE  beberapa galur Rhizopus. 
Deteksi Ganoderma secara molekuler pada kebun kelapa sawit yang diberi perlakuan biofungisida Ganor (Molecular detection of Ganoderma on oil palm plantation treated with Ganor biofungicide) Hayati MINARSIH; Happy WIDIASTUTI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.414 KB) | DOI: 10.22302/iribb.jur.mp.v1i1.289

Abstract

AbstractGanor organic fungicide potentially reduces Ganoderma, a pathogenic fungus causing basal stem rot disease. Application of Ganor on oil palm trees in the plantation attacked Ganoderma, inhibits the growth of Ganoderma fruiting bodies, improves rooting and stimulates the opening of the spear leaf. This study aims to identify molecularly the presence of Ganoderma in oil palm trees that have been attacked by Ganoderma routinely treated with Ganor for three months. Molecular analysis was performed by PCR using Ganoderma specific primers. The analysis results of sample from trunks and roots of  oil palm, indicating that the Ganoderma infected oil palm which has been treated with Ganor, were relatively free (96.4%) of Ganoderma. Of the 28 samples examined of treated plants, 27 samples did not indicate the presence of Ganoderma specific DNA band. On the other hand, the untreated oil palm trees infected by Ganoderma were still detected by the appearence of  DNA bands specific to Ganoderma. The results of molecular analysis indicated that Ganor treatments can effectively reduce the attack rate of Ganoderma in oil palm trees in the plantation infected by Ganoderma. However, the use of the molecular technique for early detection needs to be further tested to evaluate its consistency prior to introduction to the commercial growers. The reproducibility can be confirmed by repeating the experiment using more samples. Ganor effectiveness in curing oil palm trees infected by Ganoderma, maybe indicated by the ability of the reproductive organs to develop, particularly female flowers. The sex ratio of Ganor treated oil palms was clearly higher than that of control palms in 10 to 12 weeks after the treatment.[Keywords: organic fungicides, stem rot, molecular analysis, Elais guinensis Jack.] AbstrakFungisida organik Ganor berpotensi mengurangi serangan Ganoderma, cendawan patogenik penyebab penyakit busuk pangkal batang. Aplikasi Ganor pada tanaman kelapa sawit di kebun yang terserang Ganoderma, menghambat pertumbuhan tubuh buah Ganoderma, memper-baiki perakaran dan merangsang pembukaan daun tombak. Penelitian ini bertujuan untuk mengidentifikasi secara molekuler adanya Ganoderma pada tanaman kelapa sawit terserang Ganoderma yang telah mendapat perlakuan Ganor secara rutin selama tiga bulan. Analisis molekuler dilakukan dengan teknik PCR menggunakan primer DNA spesifik Ganoderma. Hasil analisis sampel batang dan akar tanaman kelapa sawit, menunjukkan bahwa tanaman Perlakuan, yaitu kelapa sawit terserang Ganoderma yang telah mendapat perlakuan Ganor, 96,4% bebas Ganoderma. Dari 28 sampel tanaman Perlakuan yang diperiksa, 27 sampel tidak menunjukkan adanya pita DNA spesfik Ganoderma. Sementara itu pada tanaman Kontrol, yaitu tanaman kelapa sawit terserang Ganoderma dan tidak mendapat perlakuan Ganor, 100% masih terdeteksi adanya Ganoderma. Dari 7 sampel tanaman kontrol yang diperiksa semuanya menunjukkan adanya pita DNA spesifik Ganoderma. Hasil analisis molekuler ini mengindikasikan bahwa perlakuan Ganor efektif mengurangi tingkat serangan Ganoderma pada tanaman kelapa sawit di kebun yang terinfeksi Ganoderma. Namun demikian, untuk lebih meyakinkan praktisi perkebunan, penggunakan teknik molekuler ini masih perlu diuji lebih lanjut terkait konsistensinya. Reprodusibilitas dapat dikonfirmasi dengan mengulangi percobaan menggunakan lebih banyak sampel. Efektivitas Ganor dalam menyehatkan tanaman kelapa sawit terserang Ganoderma ini, terindikasi juga dari perkembangan organ reproduktifnya. Sex ratio meningkat dalam waktu 10 hingga 12 minggu setelah perlakuan.[Kata Kunci:  fungisida organik, busuk pangkal  batang, analisis molekuler, Elais guinensis Jack. ]
Model Pembelajaran Learning Cycle Kooperatif Tipe STAD untuk Meningkatkan KualitasProses dan Hasil Belajar Umi Rochayati; Djoko Santoso; Muhammad Munir
Jurnal Pendidikan Teknologi dan Kejuruan Vol 22, No 1 (2014): (Mei)
Publisher : Faculty of Engineering, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jptk.v22i1.8843

Abstract

The researcher implemented the Student Centered Learning  with the following objectives: (1) to improve the achievementand the quality of the learning process inthe course of Electrical Installation and Machinery through the Cooperative Learning  cyclewith Student Teams Achievement Division (STAD); and (2) to describe the response of the students to the implemention. This study was conducted using the classroom action research approach in 2 cycles. Each cycle consisted of planning, action, observation, and reflection. The results showed: (1) the Cooperative Learning  cycle with STAD could improve the quality of the learning process, the students were not merely as the object but rather as the subject of the learning. The learning process comprised highly effective learning environment, interactive discussions, opinions sharing activities, motivation, cooperation and communication among the students. The improvement of the achievement was proved by the mean scores in the first and second cycle that were 69.05 and 76.25 respectively; and (2) the students’ feedbacksresulted from the closed questionnaire were 37.5% strongly agree and 62.5% agree to the implementation while from the open questionnaire,the feedbacks were 92.5% positive and 7.5% of the students said it was a waste of time.
Pengaruh Metode Pembelajaran Penugasan Dan Pemanfaatan Media Pembelajaran Terhadap Hasil Belajar Ajeng Nuzulia H; Djoko Santoso; Patni Ninghardjanti
JIKAP (Jurnal Informasi Dan Komunikasi Administrasi Perkantoran) Vol 2, No 1 (2017): Mei
Publisher : Program Studi Pendidikan Administrasi perkantoran FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jikap.v2i1.19542

Abstract

The research aims to know determine whether there are (1) the effect of assignment learning method on introduction administration office course in the XI graders od Administration Office Skill Competency in SMK Negeri 1 Karanganyar in the school year of 2015/2016 ; (2) the effect of utilization of learning media on introduction administration office course in the XI graders od Administration Office Skill Competency in SMK Negeri 1 Karanganyar in the school year of 2015/2016 ; (3) the effect of assignment learning method and utilization of learning media on introduction administration office course in the XI graders od Administration Office Skill Competency in SMK Negeri 1 Karanganyar in the school year of 2015/2016. This study is a quantitative research with descriptive method. The population of research is all XI graders of Administration Office Skill Competency in SMK Negeri 1 Karanganyar in the school year of 2015/2016 consisting of 71 students. The sample consisted of 59 atudents. The sampling technique used is proportional random sampling. Meanwhile technique of collecting data used is questionnaire and documentation technique. Technique of analyzing data used is statistic test with multiple regression analysis. Considering the result of data analysis, it could be conclud that (1) there was a significant effect of assignment teaching methods on learning outcomes Introduction Administrative subjects in class XI Competency Office Administration in SMK N 1 Karanganyar the school year of 2015/2016, is evident from the correlation value of 0.435> rtabel (0.235) and supported from tcount amounted to 5.273> t table (1.995) with a probability value of 0.000 means that less than 0.05 then Ho is rejected and Ha1 accepted ; (2) there is significant influence utilization of instructional media for learning outcomes Introduction Adminisrasi subjects in class XI Competence Offices Office Administration in SMK N 1 Karanganyar the school year of 2015/2016, is evident from the correlation value of 0.440> rtabel (0.235) and supported on value thitung 5.318> t table (1.995) with a probability value of 0.000 means that less than 0.05 then Ho is rejected and Ha2 accepted ; (3) there is significant influence learning method of assignment and utilization of instructional media on the results of study subjects in the class Introduction to Office Administration Office Administration XI Competency SMK N 1 Karanganyar In the school year of 2015/2016, proved of value Fhitung 25.388> F table (3 , 13) with a significance value of 0.000 at significant level of 0.05, then Ho is rejected and HA3 accepted and supported from the value of the effective contribution of the variable method of learning the assignment and utilization of instructional media contributed 42.7% to the learning outcomes of subjects Introduction to Office Administration.
Pemulihan Ekonomi Pasca Kerusuhan Tahun 1998 (Studi KasusKota Surakarta) Djoko Santoso
Unisia Vol. 31 No. 70 (2008): Jurnal Unisia
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/unisia.vol31.iss70.art6

Abstract

Economically, after the social unrest in 1998, Surakarta City has been well-known as anunlivable city. No investors are interested to invest, unless otherwise the condition changes.This research article tries to explore economic policies by Surakarta Government after theriot. Specifically, this article proposes to analyze economic condition of Surakarta Citybefore and after riot; public policies taken by Surakarta government to run economicrecovery; and recovery policies using perspective of economic education. Using qualitativeresearch, this research reveals that, there have been economic significant changes inthree to five years after riot; Surakarta government policies for economic recovery havebeen matched with public policy decision models; and the perspective of economic educationhas been applied effectively to recover Surakarta economic condition.Keywords: policy analysis, economic recovery, economic education perspective.
ANALISIS PENGARUH SIM ASET DAN KOMPETENSI TERHADAP KINERJA PENGELOLAAN ASET Yuniarti Anis Pramukawati; Indarto Indarto; Djoko Santoso
Sustainable Business Journal Vol 1, No 2 (2022): SEPTEMBER
Publisher : Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.487 KB) | DOI: 10.26623/sbj.v1i2.6755

Abstract

Aset adalah barang atau benda yang terdiri dari benda tidak bergerak dan benda bergerak baik yang berwujud (tangible) dan tidak berwujud (intangible) yang tercakup dalam aktiva / kekayaan perusahaan. Tujuan dalam penelitian ini untuk menganalisis pengaruh sistem informasi manajemen aset, kompetensi dan komitmen organisasi terhadap kinerja pengelolaan aset.. Populasi dalam penelitian ini adalah pegawai di Kantor Kecamatan Pedurungan Kota Semarang sebanyak 93 orang. Metode sample yang digunakan yaitu sampel total (total sampling) atau sensus. Hasil penelitian menjelaskan bahwa sistem informasi manajemen aset dan kompetensi berpengaruh positif dan signifikan terhadap komitmen organisasi pada Kantor Kecamatan Pedurungan Kota Semarang. Selain itu analisis lain menunjukkan bahwa sistem informasi manajemen aset, kompetensi dan komitmen organisasi berpengaruh positif dan signifikan terhadap kinerja pengelolaan aset pada Kantor Kecamatan Pedurungan Kota Semarang. Assets are goods or objects consisting of immovable objects and movable objects, both tangible and intangible, which are included in the assets / assets of the company. The purpose of this study is to analyze the effect of asset management information systems, competence and organizational commitment on asset management performance.. The population in this study were 93 employees at the Pedurungan District Office, Semarang City. The sample method used is the total sample (total sampling) or census. The primary data collection method used is by distributing questionnaires to respondents. The results of the study explain that asset and competency management information systems have a positive and significant impact on organizational commitment at the Pedurungan District Office, Semarang City. In addition, another analysis shows that the asset management information system, competence and organizational commitment have a positive and significant effect on asset management performance at the Pedurungan District Office, Semarang City.
Co-Authors . Mansyurdin . SAMANHUDI . SYAFARUDDIN A. H. SARAGIH SARAGIH A.R. PURBA PURBA Abdul Mollah S. JAYA Agus Laesanpura Agustina A. HANDAYAN Aida Farida Ajeng Nuzulia H Antonius Suwanto Anwar, Aswaldi Aprih Santoso Asmini Budiani Auzar SYARIEF B. MARAHIMIN MARAHIMIN Bambang S PURWOKO Bambang Wahyudiyono Basil J NIKOLAU Budi Sulistijo Candra Auromiqo Christantius Dwiatmadja CITRA ANDRIANI KUSUMAWAI Deden Dewantara ERIS DEDI SOLEH EFFENDI Dini Astika SARI Efendi, Darda F NURILMALA Fauziatul FITRIYAH GALUH WENING PERMATASARI Hajrial ASWIDINNOO HAJRIAL ASWIDINNOOR Hanny Hafiar Happy Widiastuti HAPPY WIDIASTUTI Hayati MINARSIH Hayati Minarsih I GEDE PUTU WIGENA Imron Riyadi Indarto Indarto Indarto Indarto SE Irma KRESNAWATY Isnu Irwantoro Jayawarsa, A.A. Ketut Jisman SINAGA K. PANJAITAN PANJAITAN M. A. GHONI GHONI Mayta Novaliza Isda Memed WIRAMIHARDJA Muhammad Jusuf Muhammad Munir Musliar Kasim Niyyah FITRANTI Niyyah FITRANTY Oktaviany Ferry TRIASTANTO Patni Ninghardjanti R.D.B LEFROY Rita Hayati Riza A PUTRANTO Riza A. PUTRANTO Roedhy Poerwanto Roesalia Anggraenie SATRIYAS ILYAS Soekarno Mismana Putra Suharyanto Sukarti Moeljopawiro Tetty CHAIDAMSARI Tri Panji Umi Rochayati VS Ariyanto Wyati Saddewisasi Yatini Y Yora FARAMITHA Yuli Budiati Yuniarti Anis Pramukawati