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All Journal JPTK: Jurnal Pendidikan Teknologi dan Kejuruan Neo-Bis Jurnal Dinamika Sosial Budaya Jurnal Riset Ekonomi dan Bisnis Menara Perkebunan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ilmiah Edunomika (JIE) SOLUSI Jurnal Tanah dan Iklim Jurnal Bingkai Ekonomi (JBE) Unisia Sustainable Business Journal
Djoko Santoso
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Deteksi Ganoderma secara molekuler pada kebun kelapa sawit yang diberi perlakuan biofungisida Ganor (Molecular detection of Ganoderma on oil palm plantation treated with Ganor biofungicide) Hayati MINARSIH; Happy WIDIASTUTI; Djoko SANTOSO
Menara Perkebunan Vol. 86 No. 1 (2018): 86 (1), 2018
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v1i1.289

Abstract

AbstractGanor organic fungicide potentially reduces Ganoderma, a pathogenic fungus causing basal stem rot disease. Application of Ganor on oil palm trees in the plantation attacked Ganoderma, inhibits the growth of Ganoderma fruiting bodies, improves rooting and stimulates the opening of the spear leaf. This study aims to identify molecularly the presence of Ganoderma in oil palm trees that have been attacked by Ganoderma routinely treated with Ganor for three months. Molecular analysis was performed by PCR using Ganoderma specific primers. The analysis results of sample from trunks and roots of  oil palm, indicating that the Ganoderma infected oil palm which has been treated with Ganor, were relatively free (96.4%) of Ganoderma. Of the 28 samples examined of treated plants, 27 samples did not indicate the presence of Ganoderma specific DNA band. On the other hand, the untreated oil palm trees infected by Ganoderma were still detected by the appearence of  DNA bands specific to Ganoderma. The results of molecular analysis indicated that Ganor treatments can effectively reduce the attack rate of Ganoderma in oil palm trees in the plantation infected by Ganoderma. However, the use of the molecular technique for early detection needs to be further tested to evaluate its consistency prior to introduction to the commercial growers. The reproducibility can be confirmed by repeating the experiment using more samples. Ganor effectiveness in curing oil palm trees infected by Ganoderma, maybe indicated by the ability of the reproductive organs to develop, particularly female flowers. The sex ratio of Ganor treated oil palms was clearly higher than that of control palms in 10 to 12 weeks after the treatment.[Keywords: organic fungicides, stem rot, molecular analysis, Elais guinensis Jack.] AbstrakFungisida organik Ganor berpotensi mengurangi serangan Ganoderma, cendawan patogenik penyebab penyakit busuk pangkal batang. Aplikasi Ganor pada tanaman kelapa sawit di kebun yang terserang Ganoderma, menghambat pertumbuhan tubuh buah Ganoderma, memper-baiki perakaran dan merangsang pembukaan daun tombak. Penelitian ini bertujuan untuk mengidentifikasi secara molekuler adanya Ganoderma pada tanaman kelapa sawit terserang Ganoderma yang telah mendapat perlakuan Ganor secara rutin selama tiga bulan. Analisis molekuler dilakukan dengan teknik PCR menggunakan primer DNA spesifik Ganoderma. Hasil analisis sampel batang dan akar tanaman kelapa sawit, menunjukkan bahwa tanaman Perlakuan, yaitu kelapa sawit terserang Ganoderma yang telah mendapat perlakuan Ganor, 96,4% bebas Ganoderma. Dari 28 sampel tanaman Perlakuan yang diperiksa, 27 sampel tidak menunjukkan adanya pita DNA spesfik Ganoderma. Sementara itu pada tanaman Kontrol, yaitu tanaman kelapa sawit terserang Ganoderma dan tidak mendapat perlakuan Ganor, 100% masih terdeteksi adanya Ganoderma. Dari 7 sampel tanaman kontrol yang diperiksa semuanya menunjukkan adanya pita DNA spesifik Ganoderma. Hasil analisis molekuler ini mengindikasikan bahwa perlakuan Ganor efektif mengurangi tingkat serangan Ganoderma pada tanaman kelapa sawit di kebun yang terinfeksi Ganoderma. Namun demikian, untuk lebih meyakinkan praktisi perkebunan, penggunakan teknik molekuler ini masih perlu diuji lebih lanjut terkait konsistensinya. Reprodusibilitas dapat dikonfirmasi dengan mengulangi percobaan menggunakan lebih banyak sampel. Efektivitas Ganor dalam menyehatkan tanaman kelapa sawit terserang Ganoderma ini, terindikasi juga dari perkembangan organ reproduktifnya. Sex ratio meningkat dalam waktu 10 hingga 12 minggu setelah perlakuan.[Kata Kunci:  fungisida organik, busuk pangkal  batang, analisis molekuler, Elais guinensis Jack. ]
Application of organic fungicide in controlling basal stem rot disease for mature oil palm Happy WIDIASTUTI; Hayati MINARSIH; Djoko SANTOSO; Deden Dewantara ERIS; Galuh Wening PERMATASARI
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.368

Abstract

Ganoderma is a major pathogen in oil palm crops. Some efforts related to control the growth of Ganoderma have been conducted but still have not found an effective method. This study aims to develop an organic fungicide that has been tested in vitro, which effective in controlling the growth of Ganoderma. The optimization carried out includes the determination of the dose and time interval for application in 13-year-old mature oil palm. This organic fungicide application was the continuation of application during the previous year especially for the two best treatment which is application organic fungicide every week (1w) and every two weeks (2w). In this study, the treatments tested were three levels dose of organic fungicide (0, 1x and 2x) and two types of frequency application, i.e. every week (1w) and every other week (2w). The results showed that the best application of organic fungicides was every week application with twice doses (1w.2x), based on the parameters of the inhibition of Ganoderma’s fruiting body formation, primary and secondary root formation, the opening of spear leaves, and harvesting parameters. The application of organic fungicide able to recover the oil palm infected Ganoderma sp., with increasing the fresh fruit bunch and its weight around 70% and 78%, respectively.
Molecular identification and phylogenetic analysis of Chlorella isolates from Indonesia using rbcL gene Fauziatul FITRIYAH; Yora FARAMITHA; Dini Astika SARI; Irma KRESNAWATY; Tri PANJI; Djoko SANTOSO
Menara Perkebunan Vol. 89 No. 1 (2021): 89 (1), 2021
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.408

Abstract

Identifying the newly isolated species is crucial to establishing a reliable algal database with successful commercial applications for different biotechnological applications. Morphological identification does not give sufficient description, especially for tiny unicellular microalgae. The rbcL gene encodes the large unit of ribulose-1, 5-bisphosphate carboxylase /oxygenase (Rubisco) has been widely known for barcoding in plants and developed for microalgae molecular identification. In this study, we examined the local strains of green microalgae from Indonesia using the rbcL partial gene sequence to identify the strains. Green microalgae isolates originated from Yogyakarta, Serayu, Gondol, Ancol, Cilegon, and Teluk Jakarta were cultured in f/2 media and harvested for DNA extraction. The DNA extracted was proceeded to PCR using 1AB_rbcL primer pair to amplify the sequences of rbcL gene with target band located at 582 bp, followed by the sequencing of the PCR product was conducted. Molecular identification of local green microalgae isolates was successfully carried out using primers 1AB_rbcL with a genetic similarity of 99% toward identified species in the NCBI database. Among six isolates, TJ, G, S, C, and A isolates were identified as C. pyrenoidosa. Only CP isolate from Yogyakarta identified as C. sorokiniana. Nannochloropsis gaditana rbcL sequence was selected as an outgroup. The phylogenetic analysis indicated that the five isolates of Chlorella belong to one clade and clearly distinguished from C. sorokiniana isolate from Yogyakarta.
Literasi Pengaruh Digital Lingkungan Kerja Dan Budaya Organisasi Terhadap Produktivitas Kerja Karyawan Melalui Kepuasan Kerja : Studi Pada Karyawan PT.EUDE Indonesia Bambang Wahyudiyono; Yuli Budiati; Djoko Santoso
Jurnal Bingkai Ekonomi (JBE) Vol 9 No 1 (2024): Januari 2024: Jurnal Bingkai Ekonomi (JBE) Januari 2024
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) - Institut Teknologi dan Bisnis (ITB) Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54066/jbe.v9i1.358

Abstract

This type of research is explanatory research. The population of this study were employees of the production department of PT. EUDE Indonesia, Semarang. The sample taken was 80 employees. The sampling technique used was a simple random sample. The data collection method used was a questionnaire. The analysis technique used is PLS (Partial Least Square). Research results show that:The work environment has a positive and significant effect on job satisfaction, Organizational culture has a positive and significant effect on job satisfaction,Job satisfaction has a positive and significant effect on work productivity,The work environment has a positive and significant effect on work productivity,Organizational culture has a positive and significant effect on work productivity.The work environment has a positive and significant effect on employee work productivity with job satisfaction as a mediating variable,Organizational culture has a positive and significant effect on employee work productivity with job satisfaction as a mediating variable.
Pengaruh Beban Kerja dan Budaya Organisasi terhadap Kinerja Pegawai dengan Lingkungan Kerja sebagai Variabel Moderasi Afif Nurhidayat; Endang Rusdianti; Djoko Santoso
Jurnal Riset Ekonomi dan Bisnis Vol. 18 No. 2 (2025): AGUSTUS
Publisher : Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/jreb.v18i2.12728

Abstract

Penelitian ini bertujuan untuk menguji dan menganalisis beban kerja, budaya organisasi dan lingkungan kerja secara langsung terhadap kinerja pegawai maupun melalui moderasi lingkungan kerja. Kemudian untuk menganalisis peran moderasi lingkungan kerja pada pengaruh beban kerja dan budaya organisasi terhadap kinerja pegawai. Metode penelitian ini yaitu kuantitatif dengan populasi sebanyak sebanyak 127 pegawai dengan teknik purposive sampling maka diperoleh sampel sebanyak 115 pegawai. Pengumpulan data menggunakan kuesioner yang disebarkan melalui googleform dan secara langsung. Data primer yang diperoleh kemudian dianalisis dengan teknik SEM menggunakan metode Partial Least Square (PLS). Hasil penelitian menunjukkan bahwa beban kerja berpengaruh negatif terhadap kinerja pegawai sedangkan budaya organisasi dan lingkungan kerja secara langsung tidak berpengaruh terhadap kinerja pegawai. Lingkungan kerja mampu memoderasi pengaruh beban kerja terhadap kinerja pegawai namun tidak mampu memoderasi pengaruh budaya organisasi terhadap kinerja pegawai Kantor Pertanahan Kabupaten Kendal, kondisi lingkungan kerja yang baik dapat memperlemah dampak negatif beban kerja tinggi sehingga pegawai tetap mampu mempertahankan kinerjanya, tetapi kualitas lingkungan kerja tidak cukup kuat untuk memperkuat pengaruh positif budaya organisasi terhadap kinerja.
PENGARUH PENGETAHUAN PERPAJAKAN, KUALITAS PELAYANAN DAN SANKSI PAJAK TERHADAP KEPATUHAN WAJIB PAJAK BADAN MENGIKUTI TAX AMNESTY DENGAN KONDISI KEUANGAN SEBAGAI VARIABEL MODERATINGNYA CITRA ANDRIANI KUSUMAWAI; Indarto SE; Djoko Santoso
Solusi Vol. 20 No. 3 (2022): July
Publisher : Fakultas Ekonomi, Universitas Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26623/slsi.v20i3.5749

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh pengetahuan perpajakan, kualitas pelayanan dan sanksi pajak terhadap kepatuhan wajib pajak badan mengikuti tax amnesty dengan kondisi keuangan sebagai variabel moderatingnya. Sampel yang digunakan sebanyak 200 wajib pajak badan di Kota Semarang sebagai client konsultan pajak yang mengikuti program tax amnesty. Metode pengumpulan sampel menggunakan purposive sampling. Penelitian ini berhasil membuktikan secara empiris bahwa (1) pengetahuan perpajakan berpengaruh positif terhadap kepatuhan wajib pajak mengikuti tax amnesty, (2) Kualitas Pelayanan berpengaruh positif terhadap kepatuhan wajib pajak mengikuti tax amnesty, (3) Sanksi perpajakan berpengaruh positif terhadap kepatuhan wajib pajak mengikuti tax amnesty, serta (4) Kondisi keuangan dapat memoderasi pengaruh pengetahuan perpajakan, kualitas pelayanan dan sanksi pajak terhadap kepatuhan wajib pajak badan mengikuti tax amnesty.Kata kunci :       Kepatuhan wajib pajak, Kondisi Keuangan, Pengetahuan Perpajakan, Kualitas Pelayanan, Sanksi Pajak
Co-Authors . SAMANHUDI . SYAFARUDDIN A. H. SARAGIH SARAGIH A.R. PURBA PURBA Abdul Mollah S. JAYA Afif Nurhidayat Agustina A. HANDAYAN Aida Farida Ajeng Nuzulia H Antonius Suwanto Aprih Santoso Asmini Budiani Aswaldi Anwar Auzar SYARIEF B. MARAHIMIN MARAHIMIN Bambang S PURWOKO Bambang Wahyudiyono Basil J NIKOLAU Candra Auromiqo Christantius Dwiatmadja CITRA ANDRIANI KUSUMAWAI Deden Dewantara ERIS DEDI SOLEH EFFENDI Dini Astika SARI Efendi, Darda Endang Rusdianti F NURILMALA Fauziatul FITRIYAH GALUH WENING PERMATASARI Hajrial ASWIDINNOO HAJRIAL ASWIDINNOOR Hanny Hafiar Happy Widiastuti HAPPY WIDIASTUTI Hayati Minarsih Hayati MINARSIH I GEDE PUTU WIGENA Imron Riyadi Indarto Indarto Indarto Indarto SE Irma KRESNAWATY Isnu Irwantoro Jayawarsa, A.A. Ketut Jisman SINAGA K. PANJAITAN PANJAITAN M. A. GHONI GHONI Mansyurdin Mayta Novaliza Isda Memed WIRAMIHARDJA Muhammad Jusuf Muhammad Munir Musliar Kasim Niyyah FITRANTI Niyyah FITRANTY Oktaviany Ferry TRIASTANTO Patni Ninghardjanti R.D.B LEFROY Rita Hayati Riza A PUTRANTO Riza A. PUTRANTO Roedhy Poerwanto Roesalia Anggraenie SATRIYAS ILYAS Soekarno Mismana Putra Suharyanto Sukarti Moeljopawiro Tetty CHAIDAMSARI Tri Panji Umi Rochayati VS Ariyanto Wyati Saddewisasi Yora FARAMITHA Yuli Budiati Yuniarti Anis Pramukawati