Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Bumi Lestari

KONSERVASI ANGGREK ALAM INDONESIA Vanda tricolor Lindl. varietas suavis MELALUI KULTUR EMBRIO SECARA IN-VITRO Rindang Dwiyani; Azis Purwantoro; Ari Indrianto; Endang Semiarti
Bumi Lestari Journal of Environment Vol 12 No 1 (2012)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for propagation of this species through in vitro culture could be nessecary for conservation purposes. High phenolic content of plant tissue is a serious problem for V. tricolor research in the laboratory, which inhibits germination and growth of the embryo. To overcome this problem, seeds were sown in a medium with the addition of tomato extract as an antioxidant. The aim of this research is to find the most suitable concentration of tomato extract for germination and growth of the embryo of V. tricolor form Bali and Merapi in order to obtained healthy seedlings for conservation purposes. Orchid pods (7 months after polination) of V. tricolor Bali and Merapi were used as plant material. The treatment consisted of 5 concentrations of tomato extract which is 0, 50 100, 150, 200, 250 g L-1. Observation was done by counting the number of protocorms for each stage of growth at 4 weeks after seed sowing. The study concluded that V. tricolor Bali is more responsive to the tomato extract compared with Merapi. Concentration of 150 gL-1 tomato extract gave the highest percentage of protocorms for Bali form, whereas Merapi form did not give significant differences either with or without tomato extract added in the culture medium.
In Vitro And Ex Vitro Propagation Of A Wild-Extinct Fern Lygodium Circinnatum (Burn.F) Sw. Grown In Bali Rindang Dwiyani
Bumi Lestari Journal of Environment Vol 16 No 2 (2016)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2016.v16.i02.p06

Abstract

Research concerning of propagation of a wild-extinct fern Lygodium circinnatum had been done at Faculty of Agriculture, Udayana University, Denpasar Bali Indonesia. At some places in Indonesia, as well as Bali, this species is used as materials for making handicraft. In Bali, the species grows wildly in the forest and it is almost extinct due to over gathering. This study aimed to find out method for domestication of L. circinnatum, therefore this wild species can be cultivated, provided materials for making handicraft and might solve the problem of extinction. Various media for growing spores of L. circinnatum in vitro and ex vitro were trialed. In conclusion, full strength of MS media without sugar was the most appropriate media for growth and development of spores of L. circinnatum in vitro. While for ex vitro, the appropriate media were paddy silt-soil and decomposed leaf either with or without addition of foliar fertilizer. However, we suggested ex-vitro cultivation was more appropriate, the technique was much easier and the spores grew faster compared to those of in-vitro.
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Bimantara, Putu Oki Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO Endang Semiarti Endang Semiarti ENDANG SEMIARTI ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Pradana, I Kadek Wira Purba, Reza Stefany Purwantoro, A - Putu Suwardike Redana, I Made Basma Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani