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All Journal PROSIDING SEMINAR KIMIA Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Food Science and Technology Journal (Foodscitech) Buletin LOUPE (Laporan Umum Penelitian) Buletin Poltanesa Jurnal Agriment Mestaka: Jurnal Pengabdian Kepada Masyarakat
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Teknologi Hasil Perkebunan, Politeknik Pertanian Negeri Samarinda
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Journal : PROSIDING SEMINAR KIMIA

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PRODUCTION OF CELLULASE AND REDUCING SUGAR FROM OIL PALM EMPTY FRUIT BUNCH BY Streptomyces hirsutus ISOLATE 12.3.A Nurkaya, Hamka
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2013
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

This research aimed to utilize the oil palm empty fruit bunch for the production of reducing sugar. The oil palm empty fruit bunch (OPEFB) is the palm oil industry waste which composed of lignocellulosic material. Utilization microbial as an organism which can degrade a lignocellulosic biomass into simple sugars is a way to reduce the environmental problem. A potential of using OPEFB to accelerate cellulase activity and reducing sugar production by cellulolytic microorganism was tested. The high cellulase activity and reducing sugar producing actinomycetes isolate 12.3.A that was isolated from the oil palm plantation area in Phetchaburi, Thailand was used in this study. The isolate 12.3.A was preliminary appointed to be Streptomyces hirsutus from the result of 16S-rRNA gene analysis. The optimal conditions for cellulase activity and reducing sugar production of S. hirsutus isolate 12.3.A were determined. The best yields were derived from culturing the cells at pH 7, 30°C, substrate concentration of 1% and ammonium sulfate as nitrogen sources for 6 days incubation. The highest cellulase activities and reducing sugar production from OPEFB as substrate were 0.71 U/ml and 3.82 mg/ml, respectively.
ETHANOL PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH AND ITS ACID HYDROLYSIS USING MICROBIAL CELLULASE SACCHARIFICATION Hamka Nurkaya
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Oil palm empty fruit bunch (OPEFB) is a co mmon solid waste fro m the palm oil industry that requires proper handling to be processed into bioethanol as new bioenergy source to substitute fossil fuel, oil, gas, and coal. Microbial applications have been considered an effective way to degrade a lignocellulosic bio mass into simple sugars as a critical material for b ioethanol production . A potential of using OPEFB and The residue of the acid hydrolysis of the OPEFB to accelerate cellulase activity and reducing sugar production of Steptomyces hirsutus isolate 12.3.A was tested. The optimal conditions for cellu lase production by S. hirsutus isolate 12.3.A were similar for both substrates. The best yields were derived fro m culturing the cells at pH 7, 30°C, and substrate concentration at 1% for 6 days and amonium sulfate is the best as a nitrogen source for OPEFB med iu m and peptone is the best as nitrogen sources for the residue medium. The highest cellulase activit ies fro m OPEFB and the acid hidrolysis residue were 0.71 U/ ml and 0.56 U/ ml, respectively. Specific activity fro m ammoniu m sulfate precipitation was raised as 3.74 U/ mg and for cellu lase purificat ion by dialysis was 7.38 U/ mg. The in itial concentration of reducing sugar for OPEFB and the residue med iu m were 1.77 g/l and 1.01 g/l while the optimal ethanol production from OPEFB and the residue as sugar sources were 0.76 g/l and 0.41 g/l at inc ubation times of 72 hours.
Co-Authors Adnan Putra Pratama Agustu Sholeh Pujokaroni Ahmad Zamroni Andi Lisnawati Asmina Sunriyani Raten Daryono Diva Aprilia Yahya Dody Purwanto Edy Wibowo Kurniawan Eko Junirianto Elisa Ginsel Popang Emmawati, Aswita Eva Numarini Eva Nurmarini Eva Nurmarini Fachry Abda El Rahman Farida Aryani Heriad Daud Salusu Husmul Beze, Husmul Irma Febriandini Khusnul Khotimah Krishna Purnawan Candra Lisa Mayanti M. Atta Bary M. Yamin Marwati Marwati Maulida Rachmawati Mika Debora Br Barus Muh. Yamin Muhammad Yamin Mujibu Rahman Netty Maria Naibaho Nur Hidayat Rindawati Rindawati Rudito Rudito Rudito Rusmini Selviana Ave Maria Funan Silvia Darmans Sri Amanah Supriono Supriono Syauqi, Anis Tere Adi Susanti Yuanita Yudha Agus Prayitno Yuliana Sabarina Lewar Yuliani, Yuliani Yulianto Yulianto Yulianto