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All Journal JBIO: jurnal biosains (the journal of biosciences) REKAYASA Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Al-Ard: Jurnal Teknik Lingkungan Jurnal Agritechno Journal of Innovation in Educational and Cultural Research Bioscientist : Jurnal Ilmiah Biologi Journal Of Sustainable Research In Management Of Agroindustry (SURIMI) PERTANIAN TROPIK Jurnal Ilmiah Membangun Desa dan Pertanian BIOTROPIC
Hanik Faizah
Biology Deparment, Faculty Sains And Technology, UIN Sunan Ampel Surabaya, Dr. Ir, H. Seokarno Street Number 682, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo Nur Rokhmatul Lailiya; Misbakhul Munir; Esti Tyastirin; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.
Pengaruh Pemberian Infusa Albedo Semangka terhadap Perubahan Ukuran Diameter Pulau Langerhans Pankreas Tikus Jantan (Rattus norvegicus) Diabetes Mellitus Widi Pangestu Wilujeng; Nurul Mahmudati; Ladiya Fitri Ma’rufah; Ninik Fadhillah; Fatmi Indah Hati; Gebby Agnessya Esa Oktavia; Aurira Thrisna Dwi Aprianti; Berlian Rustantina; Nazil Dwi Rahayuningtyas; Azizaturrohmah; Farah Annisa Nurbani; Hanik Faizah; Alfiah Hayati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1715

Abstract

Diabetes Mellitus condition (DM) correlates with the damage that occur in pancreas especially in Langerhans islets, one of the damage indicators is diameter changes of Langerhans islets that getting smaller compare with the normal sizes. The use of natural ingredients to control diabetes mellitus can be done by infusing sweet beauty watermelon albedo which can be found abundant in traditional market. Albedo layer of watermelon contain antioxidant compounds that can help to regenerate Langerhans islets of pancreas. This study purpose was to know the effect of giving watermelon’s infusion albedo to diameter of Langerhans islets in diabetes mellitus rats (Rattus norvegicus), and analyze the potential process occurred based on the result as information source for other research. This study used 25 male rats (Rattus norvegicus) as animal model with 5 groups treatment which were normal group, positive group as control (DM without treatment), A group (DM+1000 mg/kgBB watermelon’s infusions albedo), B group (DM+1500 mg/kgBB watermelon’s infusions albedo), and C group (DM+2000 mg/kgBB watermelon’s infusions albedo). After all the treatments, the rats were anesthetized and dissected and then their pancreas was taken. The pancreas was processed into histological slides by fixing the pancreas in a fixative solution (10% formalin) then dehydrated in a series of graded ethanol and embedded in paraffin wax, cut at a thickness of 4 µm, and finally stained with hematoxylin-eosin dye for microscopic observation, according to the stages Histopathological analysis of the gonads was examined using standard methods. Data was collected by measuring the size of Langerhans islets with microscope Olympus CX33  and connected to the raster image application.  Data results from this study indicate that watermelon’s infusion albedo had effect to increase the diameter of Langerhans islets of rat with diabetes mellitus
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Anggun Putri Ferdyanti; Eva Agustina; Hanik Faizah; Risa Purnamasari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Pengaruh Pemberian PGPR Bacillus SP. dan Pseudomonas SP. Asal Akar Bambu Apus terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) Hermalya Putri Hartono; Saiku Rokhim; Hanik Faizah
Jurnal Ilmiah Membangun Desa dan Pertanian Vol. 9 No. 3 (2024)
Publisher : Department of Agribusiness, Halu Oleo University Jointly with Perhimpunan Ekonomi Pertanian Indonesia - Indonesian Society of Agricultural Economics (PERHEPI/ISAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37149/jimdp.v9i3.1154

Abstract

Corn is a substitute food crop for rice, and its number continues to increase yearly. Therefore, corn has experienced an increase in productivity because many Indonesian people like corn. The supporting factor for a plant to develop well is the provision of fertilizer. The fertilizers used by Indonesian farmers are generally not environmentally friendly and damage soil nutrients, such as inorganic fertilizers or chemical fertilizers. Therefore, environmentally friendly fertilizers from living organisms are needed to increase nutrients with biological fertilizers (PGPR). The aim of this research is (1)to determine the effect of PGPR Bacillus sp. and Pseudomonas sp. from the roots of apus bamboo on the growth of corn plants, (2) knowing the comparison of variations in the concentration of PGPR Bacillus sp. and Pseudomonas sp. on corn growth. This research was carried out from January to February 2024 at the laboratory and the greenhouse of BBPPTP Surabaya. The research sample used was 21 corn plant seeds. The research variables used were plant height, root length, number of leaves, root fresh weight, leaf fresh weight, root dry weight, and leaf fresh weight. The research results were analyzed using the SPSS test (One-way ANOVA and DMRT) and presented in a table. The result showed that isolates of Bacillus sp. and Pseudomonas sp. had a natural effect on corn, while the best concentration was given at a Bacillus sp. concentration of 15% and Pseudomonas sp. 15%.
Pengaruh Penambahan Kulit Jeruk Manis Dalam Berbagai Konsentrasi Dan Lama Fermentasi Terhadap Sifat Fisik Dan Kimia Nata De Citrus Dari Sari Buah Jeruk Manis (Citrus Sinensis) sheva Matlubah; Nirmala Fitria Firdhausi; Hanik Faizah
Jurnal Agritechno Jurnal Agritechno Vol. 18, Nomor 2, Oktober 2025
Publisher : Depertemen Teknologi Pertanian Universitas Hasanuddin

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Abstract

Nata is a gel-shaped polysaccharide fermented by Acetobacter xylinum bacteria, with nata de coco as the most well-known product. The naming of nata is now adjusted to the media or fruit juice used, but the use of sweet orange juice and peel is still rarely done. This study aims to determine the effect of sweet orange peel concentration and fermentation time on the characteristics of nata from sweet orange juice. The method used is a Completely Randomized Design (CRD) and analyzed by ANOVA test. Treatment variations include sweet orange peel concentration (0.5%, 1%, and 1.5%) and fermentation time (10, 14, and 18 days). The parameters observed include physical and chemical properties. The results showed that the physical characteristics, namely the highest thickness and yield in sample E (1% sweet orange peel, 14 days fermentation) with values ​​of 2.17 mm and 18.72%, respectively. Meanwhile, the highest water content was found in sample G (0.5% sweet orange peel, 18 days fermentation) at 76.8%, and the highest cellulose content was recorded in sample J (positive control) at 3.066%.
Pengaruh Ekstrak Daun Kemuning (Murraya paniculata L.) dan Larutan Pengencer sebagai Pengawet Alami Terhadap Kualitas Kimia dan Nilai Organoleptik pada Produk Ikan Kembung (Rastrelliger kanagurta): The Effect of Orange Jasmine (Murraya paniculata L.) Leaf Extract and Diluent Solutions as Natural Preservatives on the Chemical Quality and Organoleptic Value of Indian Mackerel (Rastrelliger kanagurta) Products Elysia Zahra Helga Sandita; Misbakhul Munir; Ita Ainun Jariyah; Hanik Faizah
Journal of SURIMI (Sustainable Research In Management of Agroindustry) Vol. 6 No. 2 (2026): SURIMI : Oktober 2026
Publisher : Politeknik Negeri Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35970/mwn12p40

Abstract

Indonesia memiliki potensi sumber daya perikanan yang besar, salah satunya ikan kembung banyak dikonsumsi karena memiliki nilai gizi tinggi dengan harga terjangkau. Namun, pada aktivitas penangkapan dengan durasi lama dapat menyebabkan penurunan kualitas ikan akibat aktivitas enzim dan mikroorganisme pembusuk. Tanaman kemuning memiliki potensial dalam mencegah proses pembusukan ikan, hal ini karena memiliki kandungan antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun kemuning sebagai bahan pengawet alami ikan kembung serta variasi larutan pengencer dengan air tawar dan air laut terhadap kualitas kimia berupa kadar air dan pH, serta nilai organoleptik. Penelitian ini terdiri dari 11 perlakuan, terdiri atas kontrol negatif (ikan kembung tanpa perendaman, ikan dengan direndam air tawar, air laut), kontrol positif (pengawet komersil), ekstrak daun kemuning konsentrasi 100% (tanpa pengencer), konsentrasi 30%, 50%, dan 70% (pengencer air tawar dan air laut). Data di analisis secara statistik menggunakan Kruskal Wallis. Hasil penelitian menunjukkan nilai kadar air seluruh perlakuan masih berada pada kisaran standar mutu ikan segar, yaitu 66,6%–70%, dengan kadar air terendah pada perlakuan K1AL yaitu 66,6%, sedangkan nilai pH masih memenuhi standar kesegaran ikan, berkisar 6,26–6,46 dengan kadar pH terendah pada perlakuan K1AT 6,26. Nilai organoleptik (kenampakan rupa, bau, tekstur) tertinggi diperoleh pada perlakuan K2AL dan K3AL dengan rata-rata sebesar 7,46. Perlakuan ekstrak daun kemuning dengan pengencer air laut lebih efektif sebagai pengawet alami dibandingkan pengencer air tawar.
Rhizosphere microbial resistance test to fungicide Dimetomorph in vitro Putri Megawati Sari; Hanik Faizah; Irul Hidayati; Cici Fitri Yani
Jurnal Online Pertanian Tropik Vol. 13 No. 2 (2026): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jopt.v13i2.21669

Abstract

  Dimethomorph is a systemic fungicide utilized within Indonesian agricultural sector. Inappropriate application of dimethomorph fungicide may result in environmental contamination and the development of resistance among rhizosphere microorganisms. This study aims to ascertain the responses of rhizosphere microbial isolates to dimethomorph fungicide, thereby providing critical insights for evaluating the efficacy of this fungicide against rhizosphere microbes. Such information is essential for formulating plant pest and disease management strategies that utilize more suitable and sustainable dosages. The investigation involved testing bacterial isolates MR-B01, MR-B02, MR-B03, MR-B04, and fungal isolate MR-F01 against dimethomorph fungicide employing the agar diffusion method with disc paper. The experimental design incorporated a treatment factor with five variation concentrations of dimethomorph fungicide (0 g/L, 0.5 g/L, 1 g/L, 1.5 g/L, and 2 g/L), executed with ten repetitions. The findings indicated that varying concentrations of dimethomorph fungicide exhibited distinct effects on the resistance of the tested microbial isolates, specifically bacteria MR-B01, MR-B02, MR-B03, MR-B04, and fungal isolate MR-F01.
Co-Authors Ade Tiyan Handayani AGUSTINA, EVA Alfiah Hayati Anggun Putri Ferdyanti Anisa Shofiyana Azhari Aurira Thrisna Dwi Aprianti Azizaturrohmah Berlian Rustantina Cici Fitri Yani Edhi Sandra Eko Teguh Pribadi Elysia Zahra Helga Sandita Esti Tyastirin Evi Fatimatur Rusydiyah Fahimatul Ula Fanny Fajrin Aulia Rosyada Farah Annisa Nurbani Fatmi Indah Hati Gebby Agnessya Esa Oktavia Hermalya Putri Hartono Hernik Farisia Husniyatus Salamah Zainiyati Ikhlasotul Fawaidah Irul Hidayati Ita Ainun Jariyah Ladiya Fitri Ma’rufah Latifatoel Chilmi Mei Lina Fitri Kumalasari Millah, Najmatul Misbakhul Munir Moch. Irfan Hadi Muh. Ma’arif Muhamad Ratodi Muhammad Yusuf Alamudi Nazil Dwi Rahayuningtyas Ninik Fadhillah Nirmala Fitria Firdhausi Nur Rokhmatul Lailiya Nurul Mahmudati Putri Megawati Sari Qurrotul Ayun Reni Ida Wati Risa Purnamasari Saiku Rokhim sheva Matlubah Sri Hidayati Tatag Bagus Prakarsa Widi Pangestu Wilujeng