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All Journal JBIO: jurnal biosains (the journal of biosciences) REKAYASA Biology, Medicine, & Natural Product Chemistry Jurnal Biologi Tropis Al-Ard: Jurnal Teknik Lingkungan Journal of Innovation in Educational and Cultural Research Bioscientist : Jurnal Ilmiah Biologi BIOTROPIC
Hanik Faizah
Biology Deparment, Faculty Sains And Technology, UIN Sunan Ampel Surabaya, Dr. Ir, H. Seokarno Street Number 682, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Physics Public Health Social Sciences

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Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo Nur Rokhmatul Lailiya; Misbakhul Munir; Esti Tyastirin; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.
Pengaruh Pemberian Infusa Albedo Semangka terhadap Perubahan Ukuran Diameter Pulau Langerhans Pankreas Tikus Jantan (Rattus norvegicus) Diabetes Mellitus Widi Pangestu Wilujeng; Nurul Mahmudati; Ladiya Fitri Ma’rufah; Ninik Fadhillah; Fatmi Indah Hati; Gebby Agnessya Esa Oktavia; Aurira Thrisna Dwi Aprianti; Berlian Rustantina; Nazil Dwi Rahayuningtyas; Azizaturrohmah; Farah Annisa Nurbani; Hanik Faizah; Alfiah Hayati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1715

Abstract

Diabetes Mellitus condition (DM) correlates with the damage that occur in pancreas especially in Langerhans islets, one of the damage indicators is diameter changes of Langerhans islets that getting smaller compare with the normal sizes. The use of natural ingredients to control diabetes mellitus can be done by infusing sweet beauty watermelon albedo which can be found abundant in traditional market. Albedo layer of watermelon contain antioxidant compounds that can help to regenerate Langerhans islets of pancreas. This study purpose was to know the effect of giving watermelon’s infusion albedo to diameter of Langerhans islets in diabetes mellitus rats (Rattus norvegicus), and analyze the potential process occurred based on the result as information source for other research. This study used 25 male rats (Rattus norvegicus) as animal model with 5 groups treatment which were normal group, positive group as control (DM without treatment), A group (DM+1000 mg/kgBB watermelon’s infusions albedo), B group (DM+1500 mg/kgBB watermelon’s infusions albedo), and C group (DM+2000 mg/kgBB watermelon’s infusions albedo). After all the treatments, the rats were anesthetized and dissected and then their pancreas was taken. The pancreas was processed into histological slides by fixing the pancreas in a fixative solution (10% formalin) then dehydrated in a series of graded ethanol and embedded in paraffin wax, cut at a thickness of 4 µm, and finally stained with hematoxylin-eosin dye for microscopic observation, according to the stages Histopathological analysis of the gonads was examined using standard methods. Data was collected by measuring the size of Langerhans islets with microscope Olympus CX33  and connected to the raster image application.  Data results from this study indicate that watermelon’s infusion albedo had effect to increase the diameter of Langerhans islets of rat with diabetes mellitus
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Anggun Putri Ferdyanti; Eva Agustina; Hanik Faizah; Risa Purnamasari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Co-Authors Ade Tiyan Handayani AGUSTINA, EVA Alfiah Hayati Anggun Putri Ferdyanti Anisa Shofiyana Azhari Aurira Thrisna Dwi Aprianti Azizaturrohmah Berlian Rustantina Edhi Sandra Eko Teguh Pribadi Esti Tyastirin Evi Fatimatur Rusydiyah Fahimatul Ula Fanny Fajrin Aulia Rosyada Farah Annisa Nurbani Fatmi Indah Hati Gebby Agnessya Esa Oktavia Hernik Farisia Husniyatus Salamah Zainiyati Ikhlasotul Fawaidah Irul Hidayati Ladiya Fitri Ma’rufah Latifatoel Chilmi Mei Lina Fitri Kumalasari Millah, Najmatul Misbakhul Munir Moch. Irfan Hadi Muh. Ma’arif Muhamad Ratodi Muhammad Yusuf Alamudi Nazil Dwi Rahayuningtyas Ninik Fadhillah Nur Rokhmatul Lailiya Nurul Mahmudati Qurrotul Ayun Reni Ida Wati Risa Purnamasari Sri Hidayati Tatag Bagus Prakarsa Widi Pangestu Wilujeng