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All Journal Jurnal Kedokteran Brawijaya Journal of Innovation and Applied Technology Jurnal Pangan dan Agroindustri Jurnal Teknologi Pertanian Jurnal Sains dan Teknologi Pangan Jurnal Ilmu Pangan dan Hasil Pertanian TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Pengbdian Teknik Mesin Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
Teti Estiasih
Program Studi Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Competitive Adsorption among Phospholipids at Oil Globule Interface of Caseinate Stabilized Emulsion Estiasih, Teti
Jurnal Teknologi Pertanian Vol 13, No 1 (2012)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Casein and phospholipids are natural compounds usually used concomitantly as emulsifier. This research was conducted to elucidate the adsorbed phospholipids composition that stabilized oil globule interface during fish oil emulsification by sodium caseinate and phospholipids. Emulsion was formed by homogenizing sodium caseinate solution (10% w/v) with various phospholipids concentration of 0, 0.5, 1.0, 1.5, 2.0, and 2.5% (w/v) at fish oil concentration of 20%. The results showed that the quantity of adsorbed phospholipids increased in line with increasing phospholipids concentration. Competitive adsorption occured among various phospholipids that indicated by compositional changes of adsorbed phospholipids at oil globule interface. Their preference to adsorb was influenced by their surface activities. Among various phospholipids, phosphatidylcholine had preference to adsorb due to its higher surface activity and quantity. Phosphatidylethanolamine had less preference to adsorb because of its lower surface activity. Meanwhile, phosphatidylinositol had less ability to compete due to its low quantity. The change of adsorbed layer in oil globule interface was caused by phospholipids-casein complexation and displacement of casein by phospholipids to occupy oil globule interface. Keywords: preference to adsorb, caseinate, phospholipids, emulsion, oil globule, interface, surface activity, competitive adsorption, displacement, adsorbed layer, complexation
Urea Crystallization Optimization of Oil from Lemuru (Sardinella longiceps) Meal Processing by Product for Concentrate ω-3 Fatty Acids Preparation Estiasih, Teti
Jurnal Teknologi Pertanian Vol 11, No 1 (2010)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Oil from lemuru meal processing by product is a potential and an abundant source of ω-3 fatty acids. This oil can be processed to be ω-3 fatty acids concentrate. Urea crystallization is a method for producing high content of ω-3 fatty acids concentrate. Production of ω-3 fatty acids concentrate from lemuru oil was optimized. Central composite design of response surface method was employed as experimental design with two variables, urea-to-fatty acids ratio and crystallization time toward response of EPA+DHA content.  The result showed that quadratic regression model was suitable to explain the interaction between both variables toward response of EPA+DHA content whereas urea-to-fatty acids ratio was optimum at 2.59:1 and crystallization time was optimum at 24 hours 18 minute.  EPA+DHA content of ω-3 fatty acids concentrate in optimum condition was verified and showed the value was 57.90%, recovery yield was 34.7%, which was increased 2.51 times compared to original lemuru fish oil.  Optimum ω-3 fatty acids concentrate quality met the International Association of Fish Meal Manufacturer (IFOMA) standard except for copper concentration.Keywords: optimization, oil from lemuru meal processing by product, urea crystallization, EPA+ DHA content
Microencapsulation of Carotene Extracts from Neurospora sp. Spores With Protein Based Encapsulant Using Spray Drying Method. Pahlevi, Yusra Widya; Estiasih, Teti; Saparianti, Ella
Jurnal Teknologi Pertanian Vol 9, No 1 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Neurospora sp. produces intracellular carotenoids pigment stored in its conidia, makes orange color appearance. Carotene was extracted from spores using acetone-hexane (2:1) as solvent and virgin coconut oil (VCO) was used to facilitate dilution of carotene extract. The objective of this research was to obtain the appropriate type of encapsulant and proportion of core material to obtain good quality carotene microcapsules for food industry and health purposes.This research was conducted by randomized block design with 2 factors. The first factor was type of encapsulant (soy proteinate isolate, sodium caseinate, and whey protein isolate) and the second factor was proportion of carotene extract (20, 30 and 40% w/w on encapsulant basis).The result of this study showed that microcapsule with sodium caseinate as encapsulant and 30% proportion of carotene extract had the highest microencapsulation efficiency, total carotenes, and carotenes retention values. Whereas microcapsule with soy proteinate isolate and 20% proportion of carotene extract had the highest water content and microcapsule yield. Microcapsule with soy proteinate isolate had the highest red and yellow value, and sodium caseinate had the highest lightness value. The most stable microcapsule during storage at temperature of 70oC was microcapsule with sodium caseinate as encapsulant and 20% proportion of carotene extracts.Key words: carotene, Neurospora sp., protein based encapsulant, microencapsulation
Antioxidant Activity of Carotene-rich Sweet potato Extracted with an Hexane-Ethanol Solvent mixture Nareswari, Nadia; Estiasih, Teti; Murtini, Erni Sofia
Jurnal Teknologi Pertanian Vol 7, No 3 (2006)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

<p style="text-align: justify;">A study was conducted to assess the antioxidant activity of carotene-rich sweet potato extracted with various ratios of solvent mixture hexane and ethanol. The respective ratios of solvent mixture hexane to ethanol were 4:0, 3:1, 2:2, 1:3 and 0:4. Observations were made on the colour parameter value, beta carotene, phenol content and antioxidant activity i.e radical scavenging and peroxide inhibition activities.</p> <p style="text-align: justify;">The radical scavenging activity of the extract varied with the ratio of the solvent found at the hecane to ethanol ratio of 1:3 (89.744%), followed by the ration of 2:2 (88.539%), 4:0 (87.305%), 3.1 (86.923%) and 0:4 (83.897%). Based on the value of EC50 and the ability to react with radicals, the extract of hexane to ethanol 1:3 and 0:4 were the two best in the radical scavenging activity, as shown by the correlation coefficient of 74.9%.</p> <p style="text-align: justify;">Similarly, the peroxide formation inhibition activity also varied with the ration of the solvent i.e. 61.126% (2:2), followed by 53.035% (1:3), 50.127% (0:4), 43.561% (3:1) and 36.046% (4.0). It seems that the carotenoids were the most influenced factor to the peroxide formation inhibition activity, as shown by the correlation coefficient of 63.4%.</p><p>Keywords: Carotene-rich sweet potato, ratio of hexane:ethanol, antioxidant activity</p>
High-Tocopherol Fraction from Rice Bran (Oryza sativa) Prepared by Low-Temperature Solvent Crystallization Technique Cahyanine, Miradiah; Estiasih, Teti; Nisa, Fithri Choirun
Jurnal Teknologi Pertanian Vol 9, No 3 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Rice bran is by product form rice milling. Rice bran has good nutrition value, such as rich of vitmamin E. Indonesia has a very great amount of rice bran but has not been explored yet. The aim of this research was to know  how influence of temperatur and crystallisation duration to tocopherol rich fraction characteristic, and to determine the effective temperature and duration of crystallization in tocopherol purification from rice bran, through solvent crystallization technique in low temperature. This research was conducted by Randomized Block Design with 2 factors. First factor was crystallization temperature (0 and 10°C) and second factors was duration of crystallization (24, 30 and 36 hours). Each treatment was repeated 3 times. The result was analyzed using analysis of variance and continued by LSD and DMRT.Tocopherol content increased from rice bran oil, unsaponifiable matters, and high tocopherol fraction.  Temperature of crystallization significantly affected antioxidant activity, free fatty acid content, and peroxide value.  Time of crystallization affected tocopherol concentration, antioxidant activity, and peroxide value.  The best treatment was obtained from temperature of crystallization of 0°C and crystallization time of 24 hours.   The characteristics of this fraction was tocopherol concentration of 17.84%, antioxidant activity of 38.42%, free fatty acid content of 2.28%, peroxide value of 6.45 meq/kg, and color value of 100.Keywords: tocopherol, rice bran, crystalization, solvent, unsaponifiable fraction
Functional Properties of Pre-gelatinization Red Glutinous Rice Muchlisyiyah, Jhauharotul; Prasmita, Hera Sisca; Estiasih, Teti; Laeliocattleya, Rosalina Ariesta
Jurnal Teknologi Pertanian Vol 17, No 3 (2016)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Ketan merah berasal dari daerah Pacitan, Jawa Timur. Ketan merah  biasa digunakan sebagai tetel ketan merah, tape ketan merah, rengginang atau sekedar direbus dan ditaburi kelapa parut. Ketan merah memiliki keunggulan memiliki senyawa fenol yang tinggi. Kelemahannya, ketan merah memiliki sifat yang sulit mengembang di dalam air dingin. Pragelatinisasi ditujukan untuk memperbaiki sifat fungsional dari ketan merah. Penelitian ini dilakukan dengan Rancangan Acak Kelompok (RAK) dengan dua faktor. Masing-masing faktor terdiri dari tiga level yaitu untuk faktor suhu pemanasan 50oC, 60oC dan 70oC. Faktor yang kedua yaitu lama pemanasan 5 menit, 7,5 menit, dan 10 menit. Masing-masing satuan percobaan dilakukan tiga kali ulangan. Hasil penelitian menunjukkan bahwa pragelatinisasi dapat memperbaiki nilai daya serap air, indeks kelarutan, kemampuan mengembang (swelling properties), dan nilai total fenol dari tepung ketan merah.Ketan merah berasal dari daerah Pacitan, Jawa Timur. Ketan merah  biasa digunakan se-bagai tetel ketan merah, tape ketan merah, rengginang, atau ketan rebus dengan taburan kelapa parut. Ketan merah memiliki keunggulan memiliki senyawa fenol yang tinggi. Kelemahannya, ketan merah memiliki sifat yang sulit mengembang di dalam air dingin. Pragelatinisasi ditujukan untuk memperbaiki sifat fungsional dari ketan merah. Penelitian ini menggunakan metode ek-sperimen dengan Rancangan Acak Kelompok (RAK) dengan dua faktor. Masing-masing faktor terdiri dari tiga level yaitu untuk faktor suhu pemanasan 50 °C, 60 °C, dan 70 °C. Faktor yang kedua yaitu lama pemanasan 5 menit, 7.5 menit, dan 10 menit. Masing-masing satuan percobaan dilakukan tiga kali ulangan. Perlakuan terbaik dari tepung ketan merah pragelatinisasi dida-patkan dengan pemanasan pada suhu 60 °C selama 10 menit dengan karakteristik daya serap air sebesar 2.45 g/g, daya serap minyak 2.02 g/g, swelling poperties 2.39 g/g, indeks kelarutan 0.0050%, serta total fenol 624.86 mg GAE /100 g
Application of Dry Colorant Containing Antioxidant from the Waste of Tea Processing for Hypoglicaemic Biscuit Substituted by Suweg (Amorphophallus campanulatus) Flour Faidah, Nielma Nur; Estiasih, Teti
Jurnal Teknologi Pertanian Vol 10, No 3 (2009)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The waste of tea extraction has high content of polyphenol.  This waste can be processed to be natural brown food colorant that contains antioxidant.  Foam mat drying is a suitable method for producing natural brown food colorant.  Application of this food dye can be used as colorant agent in hipoglycemic biscuit that does not cause the increase of glucose blood level after consumption.  Wheat flour in the making of the biscuit was subtituted by suweg flour.  The suweg flour had lower glycaemic index than wheat flour.  The combination of natural brown food colorant contained antioxidant and suweg flour subtitution is expected to give functional effect for hypoglycaemic biscuit to prevent the increase of glucose blood level and has antioxidant property.The research comprised of two steps.  First step was dry colorant preparation and the second was application of dry colorant for hypoglycaemic biscuit.  The randomized block design was employed for each step with two variabels for each.  Experiment one: the variables were maltodextrin concentration (6%, 18% b/v) and egg white concentration (1%, 4%, 7% b/v). Experiment two: the variables were dry colorant concentration (2.5%, 5% b/b) and suweg flour-to-wheat flour subtitution level (8%, 24%, 40% b/b).   In the second experiment, hypoglycaemic biscuit was tested to wistar rats for determining its effectiveness in reducing blood glucose level compared to control.The result showed that the addition of maltodextrin in dry colorant preparatiom significantly affected antioxidant activity, total phenolic content, color, solubility, and water vapor absorption.  The best treatment of dry colorant based on physical and chemical parameters was 18% of maltodextrin concentration and 1% of egg white addition.  The characteristics of this dry colorant were as followed: 37.78% of antioxidant activity, 24.12 ppm of total phenolic content, 51.4 of brightness level, 0.074 g/sec of solubility, 8.03% of  water vapor absorption.  Meanwhile, the addition of egg white significantly affected antioxidant activity, total phenolic content, and color.  Subtitution of wheat flour by suweg flour significantly influenced biscuit color and reduced blood glucose level of rats.  The best treatment of hypoglycaemic biscuit based on physical, chemical, and bioassay parameters was obtained in 5% dry colorant addition and 10% suweg flour-to-wheat flour substitution level, with characteristics as followed: 35.95% of antioxidant activity, 3.8 ppm of  total penolic content, 44.4 of brightness level, 28 mg/dl of blood glucose level reduction.Keywords: the waste of tea extraction, dry colorant containing antioxidant, suweg flour, hipoglycaemic biscuit
Hypocholesterolemics Effects of Yam Tuber (Dioscorea hispida Dennst) flour on Male Wistar Rat with Hypercholesterol Diet Maligan, Jaya Mahar; Estiasih, Teti; Sunarharum, Wenny Bekti; Rianto, Thomas
Jurnal Teknologi Pertanian Vol 12, No 2 (2011)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Yam tuber (Dioscorea hispida Dennst) contains water soluble polysaccharides.  Itis supposed that water soluble polysaccharides from yam functions as bioactivecompound to decrease blood cholesterol level. The hypocholesterolemic effect of watersoluble polysaccharides in yam tuber (Dioscorea hispida Dennst) flour on Wistar rats(Rattus norvegicus) lipid profile was investigated.   A two month old Wistar rats (Rattusnorvegicus) consisted of three groups (P0, P1 and P2) with five rats of each group wereadapted for one week by giving standard diet (AIN-93). P0 group was treated bystandard diet AIN-93M, P1 group was treated by standard diet and force feeding by eggyolk as cholesterol source, whereas P2 were given modified standard diet (AIN-93M withyam tuber flour). The weight and cholesterol level were measured in 4 weeks. Bloodwas drawn from eyes (retro orbital plexus) every week for analyzing cholesterol levels(total cholesterol, HDL, LDL cholesterol and triglycerides). Research showed thatstandard diet (AIN-93M) which is modified with yam tuber flour (P2) could decrease totalcholesterol as much as 15.37 mg/dL, 18.85 mg/dL of total triglycerides, and 14.24mg/dL of LDL-c level. Conversely this modified diet can increase the HDL-c level asmuch as 27.20 mg/dL.Keywords: hypocholesterolaemic, yam tuber flour, lipid profile, hypercholesterol diet,wistar rat
The Potential of Bioactive Compounds from Corn Silk (Zea mays L.) that Result from Gradual Fractionation Using Organic Solvents for the Use as a Natural Sunscreen Laeliocattleya, Rosalina Ariesta; Prasiddha, Ismizana Jati; Estiasih, Teti; Maligan, Jaya Mahar; Muchlisyiyah, Jhauharotul
Jurnal Teknologi Pertanian Vol 15, No 3 (2014)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

The study aims to determine how the influence of the solvent on the content of bioactive compounds such as phenols, flavonoids, and carotene and also SPF (Sun Protection Factor) value on corn silk extract and how its potential for natural sunscreen. Dried corn silk was macerated using ethanol 96%, extract was fractionated then obtained extract from the fraction of: ethanol soluble (E1), ethanol soluble – n-hexane soluble (E2), ethanol soluble – ethyl acetate soluble (E3), and ethanol soluble – water soluble (E4). Total phenolic contents respectively was 34029.37 ± 1926.61 mg/kg; 358.28 ± 119.79 mg/kg; 9569.64 ± 1494.01 mg/kg; 41751.41 ± 1390.41 mg/kg. Total flavonoid contents respectively was 211.05 ± 3.73 mg/kg; 0 mg/kg; 36.31 ± 3.85 mg/kg; and 274.73 ± 9.24 mg/kg. Total carotene contents respectively was 11.3 ± 0.95 mg/kg; 434.68 ± 86.5 mg/kg; 41.18 ± 7.08 mg/kg; and 3.97 ± 0.41. SPF value determination by In Vitro spectrophotometry performed at two different concentrations (100 ppm and 1000 ppm), there are also controls in the form of commercial sunscreen (K1), β-carotene (K2), and quercetin (K3). The result showed that higher concentration resulted in a higher SPF value. SPF value (1000 ppm) consecutively was 17.30 ± 0.15; 9.97 ± 1.11; 25.38 ± 2.88; and 16.88 ± 2.09 and the controls consecutively was 31.00 ± 0.36; 39.35 ± 0.10; and 39.20 ± 0.06. The presence of various solvents influences on the content of bioactive compounds and also SPF values in each fraction. Although the SPF value from corn silk were lower compared to the controls. However, it is known that corn silk can be used for sunscreen as it belongs to type of ultra protection with SPF>15.Keywords: sunscreen, corn silk, bioactive compounds, SPF
Antioxidant Activity of Extracts Obtained by Applying Various Solvents to the Local Brown Sorghum (Sorghum bicolor) Bran Dewi, Juwita Ratna; Estiasih, Teti; Murtini, Erni Sofia
Jurnal Teknologi Pertanian Vol 8, No 3 (2007)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

A completely randomized design experiment was conducted to determine the antioxidant activity of bran extracts obtained by applying various solvents to the local brown sorghum grain. Four types of technical grade of solvents were employed, namely aquadest, ethanol 96%, methanol 98% and acetone 98%. Each bran extract was obtained by shaking for 8 hrs in a single solvent, respectively. The extract was vacuum dried prior to use. The antioxidant activity of the extract was assessed by determining its ability for free radicals (DPPH) scavenging and for the ability for oxidation inhibition of a particular fatty acid with ferric thiocyanate.The results indicated that the levels of phenols, tannin and anthocyanin, varied with the solvent and the methanol extract contained the highest level of total phenols (55.7%). The highest radical scavenging activity was found at a concentration of 400 ppm. The methanol extract (400 ppm) showed the highest EC50 value (81,5 %), which was slightly lower than that of BHT. The order of oxidative inhibition, from the highest one, was the extracts of methanol > ethanol > acetone > aquadest. The value of the methanol extract was almost comparable the one of BHT. Both the scavenging activity and oxidative inhibition were correlated positively with the levels of tannin and other phenols, but negatively correlated with the anthocyanin content.Keywords: brown sorghum, bran extracts, antioxidant activity
Co-Authors -, Yunianta A. Harijono Abdur Rohim, Abdur Adila, Lutfia Agustin, Rivana - Ahmad Zaki Mubarok, Ahmad Zaki Alfi Khatib, Alfi Ambar Fidyasari Andriani Kusumawati Angky Wahyu Putranto Anugerah Dani Priyanto Anugerah Dany Priyanto, Anugerah Dany Apriliastuti Apriliastuti Arya Ulilalbab Asusti Asusti, Asusti Avida Nur Hidayah, Avida Nur Dego Yusa Ali Delicia Kusuma, Delicia Dian Widya Ningtyas Dr.Ir. Yunianta, DEA Ella Saparianti Endrika Widyastuti Ernawati Ernawati Erni Sofia Murtini Erryana Martati Eva Sofia Fairuz Balqis, Fairuz Fithri Choirun Nisa Hafiz Iqbal Maulana Hanifah Rosyada, Hanifah HARI PURNOMO Hera Sisca Prasmita, Hera Sisca I Gusti Ngurah Pratama Putra, I Gusti Ngurah Pratama Indria Purwantiningrum Ines Caesarina, Ines Ismizana Jati Prasiddha Jaya Mahar Maligan Jhauharotul Muchlisyiyah, Jhauharotul Juwita Ratna Dewi Kgs Ahmadi Khoirun Nisa Krisna, Agustin Lina Novi Ariani, Lina Novi M. Wahyu Agung Prasetya, M. Wahyu Agung Miftahurrahmi, Miftahurrahmi Miradiah Cahyanine Muhammad Naufal Arisyi, Muhammad Naufal Nadia Nareswari Nielma Nur Faidah Novita Wijayanti Nur Ida Panca Nugrahini Olivia Yofananda, Olivia Praditya, Brilliant Puspita, Riska Ramadhani, Faiz Ratna Yulistiani Rayesa, Neza Fadia Retno Astuti Ricca Claudia Rini Yulianingsih Rizki Mukti Adicandra, Rizki Mukti Rodhia Dara Albike, Rodhia Dara Rosalina Ariesta Laeliocattleya, Rosalina Ariesta SATRIYAS ILYAS Sirinupong, Nualpun Siti Fatimatul Mutmainah, Siti Fatimatul Siti Narsito Wulan Sudarma Dita Wijayanti Thomas Rianto Tri Sulistyo Tunjung Mahatmanto Wenny Bekti Sunarharum Widyasari wulansari, angela Yusra Widya Pahlevi Zamnia Wahyuli, Zamnia