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Exploring the Genetic Landscape of Octopus cyanea: Connectivity and Population Structure in Northern Waters of Sumatra Ramadhaniaty, Mutia; Damora, Adrian; Moula, Intania; Fadli, Nur; Razi, Nanda Muhammad; Muchlisin, Zainal A
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.529-539

Octopus cyanea is an ecologically and economically important cephalopod with wide larval dispersal influenced by ocean currents. However, geographic isolation among island clusters in northern Sumatra may restrict gene flow and shape population structure. This study investigated the genetic diversity and connectivity of O. cyanea from five populations (Aceh Island, Weh Island, Banyak Island, Simeulue Island, and Nias Island) using mitochondrial cytochrome c oxidase subunit I (COI) sequences. A total of 21 individuals were successfully amplified, yielding 651 bp fragments. Sequence analysis revealed 11 haplotypes across populations, with haplotype diversity (Hd) ranging from 0.285 in Banyak Island to 0.800 in Aceh Island, and nucleotide diversity (π) between 0.001 and 0.009. Pairwise genetic distances varied from 0.000 to 0.004, with the highest divergence observed between Simeulue Island and Nias Island (0.004), while Aceh Island and Weh Island showed no differentiation. AMOVA indicated that most genetic variation occurred within populations (68.74%), with an overall Fst of 0.315, suggesting low to moderate genetic structuring. The haplotype network demonstrated shared haplotypes among four populations, whereas Simeulue Island harbored unique haplotypes, indicating partial genetic isolation. These results highlight substantial genetic connectivity among O. cyanea populations in northern Sumatra, facilitated by larval dispersal via ocean currents, but also emphasize localized divergence in Simeulue Island. The findings provide critical baseline data for developing ecosystem-based fisheries management, supporting shared stock strategies while accounting for population-specific conservation needs.

New Distribution Record and Genetic Relationship of Bottlenose Wedgefish (Rhynchobatus australiae) Found in the Waters of Meulaboh, West Aceh Bahri, Samsul; Arjayanda, Elsa; Fadli, Nur; Ramadhaniaty, Mutia; Razi, Nanda M.; Erima, Derila; Hasibuan, Muhammad B.A.F.
Aceh Journal of Animal Science Vol 10, No 3 (2025): December 2025
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.10.3.46165

Bottlenose Wedgefishes are fish that belong to the subclass Elasmobranchii, which are cartilaginous fish. West Aceh waters are one of the areas directly connected to the Indian Ocean. Bottlenose Wedgefish can be found in the Indian Ocean. Some information on Bottlenose Wedgefish DNA has now developed molecular approaches, which provide a fast and precise alternative to identify species. This research activity aims to report the presence of Bottlenose Wedgefish sharks (Rhynchobatus australiae) and analyze the relationship between Bottlenose Wedgefish sharks found in West Aceh with other regions. This research was conducted from October to December 2021. The specimens were taken from the Ujong Baroh Fish Landing Site (PPI), West Aceh, and analyzed at the Laboratory of Genetics and Aquatic Biodiversity, Faculty of Marine and Fisheries, Syiah Kuala University. The results of the study resulted in several conclusions, namely the Query Cover value of Meulaboh 1 and Meulaboh 2 samples of 93%. The Ident value of 99.70%-99.85% indicates that the same species is R. australiae. Thymine (T) nucleotide composition is 30.8%, Cytosine (C) is 24.9%, Adenine (A) is 27.4%, and Guanine (G) is 16.9%. Based on the results of phylogenetic tree reconstruction, the relationship between Rhynchobatus australiae Meulaboh samples 1 and 2 has a close kinship with Rhynchobatus australiae from India1, India2, India3, Malaysia1, Indonesia3, and Indonesia5. This research confirms the presence of Rhynchobatus australiae in West Aceh and establishes a close genetic link to regional populations.

Effect of water acidity on the growth performance, survival, and hematology condition of the barramundi fish Lates calcarifer (Bloch, 1790) fingerling Mustika Marzah Fitriana; Nur Fadli; Zainal Abidin Muchlisin
Depik Vol 12, No 1 (2023): APRIL 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.1.31246

Global warming is caused by increased carbon emissions into the atmosphere resulting from burning oil, gas, and other fossil fuels. Subsequently, the carbon gas enters the waters through a diffusion process facilitated by the concentration of gases in the air, which is higher than in the waters. The outcome of this process is a decrease in water acidity, leading to a lower pH, which can disrupt the life of aquatic biotas. Therefore, this study aims to analyze the effect of decreasing of pH on the growth, survival, and physiological conditions of barramundi (Lates calcarifer). To achieve this objective, seven pH levels were tested, namely pH of 7.24 (control), pH 6.74, pH 6.24, pH 5.74, pH 5.24, pH 4.74, and pH 4.24. Every treatment was performed with four replications, and the fish was reared for 30 days in the respective tested pH. The reared madia is sea water with a salinity of 22 ppt. The experiment was conducted in the laboratory of fish breeding Faculty of Marine and Fisheries, Universitas Syiah. The results showed that a decreasing in pH had a significant effect on the growth performance and hematological condition of barramundi (P0.05), but its had no significant effect on survival (P0.05). The experimental fish could survive at pH 4.24, but their growth and hematological conditions were disrupted below 6.24. Therefore, it was concluded that the lower threshold value of pH for barramundi was 6.24.Keywords:Global warmingOcean aciditypHFisheries productionPhysiological disturbance

Molecular identification of sharks and rays species from Aceh waters, Indonesia Mutia Ramadhaniaty; Maria Ulfah; Indra Indra; Nur Fadli; Nanda Muhammad Razi
Depik Vol 12, No 1 (2023): APRIL 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.1.29136

Shark is a cartilaginous fish that is widely hunted because it has high economic value. The waters of Aceh are directly adjacent to the Indian Ocean and the Malacca Strait making it a preferred habitat for pelagic fish, especially sharks. Information on shark species landed in the waters west and east of Aceh is very limited due to difficulties in identification and commonly used local names. This study aimed to determine the types of sharks landed in Banda Aceh, Meulaboh, Langsa, Southwest Aceh and Simeulu and to find out specifically what species of sharks are most often landed in Aceh waters. Primary data gathering consisted of four stages, namely sample collection, identification using identification books, molecular identification cytochrome oxidase subunit I (COI) and phylogenetic analysis. The results of the molecular analysis of 46 tissue samples from five locations identified 13 species of sharks, namely Carcharhinhus sorrah, Carcharhinhus amboinensis, Triaenodon obesus, Isurus oxyrinchus, Sphyrna zygaena, Sphyrna lewini, Loxodon macrorhinus, Hemipristis elongaria, Stagostoma fasciatum, Nebrius ferrugineus, Chilloscyllium punctatum, Isurus oxyrinchus, Alopias pelagicus, Alopias supercillosus and 1 species of rays, namely Rhynchobatus australiae. Phylogenetic tree reconstruction using the Neighbor Joining method of 610 basepairs consisting of two large clades separates the species Alopias pelagicus and Isurus oxyrichus with Carcharhinus sorrah, Sphyrna lewini, Loxodon macrorhinus and Rhyncobatus australiae with boostrap values of 87% and 64%. The haplotype diversity shown ranged from 0.667-0.889 while the nucleotide diversity ranged from 0.001-0.097. These values indicates high diversity because of the variance in the number of species found.Keywords:BarcodeCOISharks catchAlopias supercillosusSphyrna lewini

Effect of water acidity on the growth performance, survival, and hematology condition of the barramundi fish Lates calcarifer (Bloch, 1790) fingerling Mustika Marzah Fitriana; Nur Fadli; Zainal Abidin Muchlisin
Depik Vol 12, No 1 (2023): APRIL 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.1.31246

Global warming is caused by increased carbon emissions into the atmosphere resulting from burning oil, gas, and other fossil fuels. Subsequently, the carbon gas enters the waters through a diffusion process facilitated by the concentration of gases in the air, which is higher than in the waters. The outcome of this process is a decrease in water acidity, leading to a lower pH, which can disrupt the life of aquatic biotas. Therefore, this study aims to analyze the effect of decreasing of pH on the growth, survival, and physiological conditions of barramundi (Lates calcarifer). To achieve this objective, seven pH levels were tested, namely pH of 7.24 (control), pH 6.74, pH 6.24, pH 5.74, pH 5.24, pH 4.74, and pH 4.24. Every treatment was performed with four replications, and the fish was reared for 30 days in the respective tested pH. The reared madia is sea water with a salinity of 22 ppt. The experiment was conducted in the laboratory of fish breeding Faculty of Marine and Fisheries, Universitas Syiah. The results showed that a decreasing in pH had a significant effect on the growth performance and hematological condition of barramundi (P0.05), but its had no significant effect on survival (P0.05). The experimental fish could survive at pH 4.24, but their growth and hematological conditions were disrupted below 6.24. Therefore, it was concluded that the lower threshold value of pH for barramundi was 6.24.Keywords:Global warmingOcean aciditypHFisheries productionPhysiological disturbance

Molecular identification of sharks and rays species from Aceh waters, Indonesia Mutia Ramadhaniaty; Maria Ulfah; Indra Indra; Nur Fadli; Nanda Muhammad Razi
Depik Vol 12, No 1 (2023): APRIL 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.1.29136

Shark is a cartilaginous fish that is widely hunted because it has high economic value. The waters of Aceh are directly adjacent to the Indian Ocean and the Malacca Strait making it a preferred habitat for pelagic fish, especially sharks. Information on shark species landed in the waters west and east of Aceh is very limited due to difficulties in identification and commonly used local names. This study aimed to determine the types of sharks landed in Banda Aceh, Meulaboh, Langsa, Southwest Aceh and Simeulu and to find out specifically what species of sharks are most often landed in Aceh waters. Primary data gathering consisted of four stages, namely sample collection, identification using identification books, molecular identification cytochrome oxidase subunit I (COI) and phylogenetic analysis. The results of the molecular analysis of 46 tissue samples from five locations identified 13 species of sharks, namely Carcharhinhus sorrah, Carcharhinhus amboinensis, Triaenodon obesus, Isurus oxyrinchus, Sphyrna zygaena, Sphyrna lewini, Loxodon macrorhinus, Hemipristis elongaria, Stagostoma fasciatum, Nebrius ferrugineus, Chilloscyllium punctatum, Isurus oxyrinchus, Alopias pelagicus, Alopias supercillosus and 1 species of rays, namely Rhynchobatus australiae. Phylogenetic tree reconstruction using the Neighbor Joining method of 610 basepairs consisting of two large clades separates the species Alopias pelagicus and Isurus oxyrichus with Carcharhinus sorrah, Sphyrna lewini, Loxodon macrorhinus and Rhyncobatus australiae with boostrap values of 87% and 64%. The haplotype diversity shown ranged from 0.667-0.889 while the nucleotide diversity ranged from 0.001-0.097. These values indicates high diversity because of the variance in the number of species found.Keywords:BarcodeCOISharks catchAlopias supercillosusSphyrna lewini

Diversity of commercially important grouper (Family: Epinephelidae) in Simeulue and Banyak Islands, Aceh, Indonesia Nanda Muhammad Razi; Zainal A. Muchlisin; Mutia Ramadhaniaty; Adrian Damora; Firman M. Nur; Mohd Nor Siti-Azizah; Nur Fadli
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.23790

Grouper is one of the economically important fish groups in the Simeulue and Banyak Islands. However, data on the diversity of the grouper in this region is still limited. This study aimed to inventory the commercially important grouper in Simeulue and Banyak Islands. Sampling was carried out in April - September 2021 at sixteen locations around Simeulue and Banyak Islands. In total, 20 species of commercial grouper consisting of 6 genera were recorded during the study. Epinephelus was the most dominant genus with ten species, followed by Cephalopholis with four species. In addition, seventeen species (85%) were included in the Least Concern (LC), two species (10%) were categorized as Vulnerable (VU), and one species (5%) was Data Deficient (DD). This study provides a database of commercially important grouper in Simeulue and Banyak Islands that can be used to manage the fish group.

Grouper DNA barcoding studies in Indonesia: A short review Nanda Muhammad Razi; Zainal A. Muchlisin; Siti Maulida; Mutia Ramadhaniaty; Firman M. Nur; Adrian Damora; Sumarni Laila Buang Manalu; Nur Fadli
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.2.21255

Indonesia is recognized as one of the territories that have the highest reef fish biodiversity in the world. One of the commercially valuable fish in this area is the groupers (locally name "kerapu"). At least 76 grouper species have been reported in Indonesian waters, with three species were categorized into "vulnerable", five species "Data Deficient", and 68 species under the "Least Concern" category based on IUCN classification. The increasing exploitations rate had been reported caused the grouper stocks in Indonesia to decrease and threatened extinction. However, only limited scientific data is available regarding the grouper in Indonesia, including their identification. In most fish landing sites across Indonesia, the groupers are morphologically identified and recorded as "kerapu" to replace their scientific species names. Accurate species identification is essential in designing appropriate and sustainable management of fisheries resources. One of the tools that have been used in fish identification is DNA barcoding. In the last two decades, this molecular method has been applied to identify many fish groups globally, including grouper fish. This study reviewed the DNA barcoding approach in grouper identification in Indonesia based on the available literature.Keywords:DNA barcodingGrouperMolecular TaxonomyFisheriesIndonesia

Critical thermal limit and behavior of the Barramundi (Lates calcarifer Bloch, 1790) fingerling exposure with different temperature Fachrurozi Amir; Firman M. Nur; Nur Fadli; Zainal Abidin Muchlisin
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.20287

Barramundi (Lates calcarifer) is one of the commercial fish in Indonesia. The phenomenon of climate change due to global warming has affected the life of aquatic biota, including fish. The objectives of the present study were to determine a critical thermal maximum (CTMax) and the behavior of Barramundi (L. calcarifer) due to the increase in water temperature. The research was conducted at the Fishing Technology Laboratory, Faculty of Marine and Fisheries, Universitas Syiah Kuala in February 2021. The experiment was conducted in 10 aquariums equipped with heaters. The initial temperature of the treatment was 28oC and increased gradually every one minute by 0.2oC. The results showed that the abnormal behavior of Barramundi fingerling was started to appear at 37oC where the fish began to swim fast, then swim sideways at 38oC-39oC, hit the container wall at 39oC-40oC, jumped and weakened then dead at 40oC and 41oC. The average critical temperature of Barramundi fingerling was recorded at a temperature of 41oC within 65 minutes. Therefore, it is concluded that the Barramundi (L. calcarifer) display an abnormal behavior above the temperature of 35oC, however, they can survive up to 42oC exposure for 70 minutes.Keywords:Critical thermal maximumTemperature riseGlobal warming

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