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Journal : Jurnal Veteriner

Aktivitas Antiproliferasi Ekstrak Etanol Biji Kopi Hijau Robusta Lampung pada Sel Lestari Tumor Anjing Farra Sasmita; Ietje Wientarsih; Bayu Febram Prasetyo; Bambang Pontjo Priosoeryanto
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.353 KB) | DOI: 10.19087/jveteriner.2021.22.1.133

Abstract

This study aim at determining the activity of ethanol extract of Robusta green coffee beans as an antiproliferation agent in MCA-B1, MCM-B2, and Vero normal cell lines as a control. Ethanol extract of Robusta green coffee beans was obtained by maceration method, bioactive compounds in the extract were tested by phytochemical screening methods, testing levels of antioxidants were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, cytotoxicity effects (LC50) were tested by the method Brine Shrimp Lethallity Test (BSLT) and antiproliferation activity were carried out by counting the number of cells after being tested with several extract concentrations namely 100, 250, 400, 550, 700 and 850 ppm and doxorubicin as a positive control with a concentration of 100 ppm. The results showed that the ethanol extract of Robusta green coffee beans contained flavonoids and tannins, had very strong antioxidant levels with IC50 values of 40.9923 ppm, cytotoxicity effects obtained LC50 values of 430.64 ppm, and the highest antiproliferation activity was achieved at a concentration of 850 ppm at 69.58 % in MCM-B2 tumor cells, 60.46% in MCA-B1 tumor cells, and 14.2% in Vero normal cells. Based on the results, the ethanol extract of Robusta Lampung green coffee beans has antiproliferation activity on the test tumor cells and is relatively non-toxic to normal cells.
Aktivitas Sediaan Gel Ekstrak Batang Pohon Pisang Ambon dalam Proses Penyembuhan Luka pada Mencit Bayu Febram Prasetyo; Ietje Wientarsih; Bambang Pontjo Priosoeryanto
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.19 KB)

Abstract

The purpose of this study is to examine the activity of Ambon banana extract in gel formulation on thewound healing process of mice skin (Mus musculus albinus) based on gross and microscopic observations.The wound healing process were observe grossly everyday while the microscopic lesion was observed on the3rd, 5th, 7th, 14th and 21st days after skin incisition. The wounded skin were sampled after the mice wereeuthanized for further microscopic observation. The gross parameters were the existence of blood coagulation,scab formation, wound covering and wound size. The microscopic parameters observed the percentation ofwound reepitelization and the the tickness of wound connective tissues (fibroblast). Gross lesion and thefibroblast thickness were presented descriptively as a qualitative data. Grossly, it was observed that thescab formation was faster in mice treated with the extract of ambon banana than that of negative control.Based on the research the ambon banana extrack in gel can be used in the acceleratiof wound healing.
Uji Aktivitas Antioksidan Daging Buah Bisbul dan Batang Buah Naga Merah Menggunakan Metode 1,1-Diphenyl-2-Picrylhydrazin Ietje Wientarsih; Bayu Febram Prasetyo; Ahmad Kurniawan; Fikri Hanifah
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.082 KB) | DOI: 10.19087/jveteriner.2020.21.4.596

Abstract

Bisbul (Diospyros discolor Willd.) is a plant of the Ebenaceae family and red dragon (Hylocereus polyrhizus) is a plant of the Cactaceae family. Bisbul and red dragon have bioactive compounds are flavonoids that act as antioxidants. The purpose of this study was measure antioxidant activity from ethanol extract of bisbul pulp and ethanol extract of red dragon fruit stem by DPPH method (1,1-diphenyl-2-picrylhydrazin). Extraction by maseration method and using 96% ethanol as solution. Antioxidant activity assay using the immersion method of DPPH measured absorption at a wavelenght 517 nm and compared with vitamin C. The result showed that the ethanol extract of bisbul pulp (IC50 46.00 ìg/mL) more strong antioxidant activity compared the ethanol extract of red dragon fruit stem (IC50 1020.96 ìg/mL).
Studi Penggunaan Obat Anti Ektoparasit di Salah Satu Klinik Hewan Kota Bogor Tahun 2017 dan 2018 Bayu Febram Prasetyo; Harry Soehartono; Rahmalia Dini Hanifa
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.798 KB) | DOI: 10.19087/jveteriner.2022.23.2.259

Abstract

The anti-ectoparasitic drug is the type of medication used to treat infections caused by various kinds of ectoparasites. The use of this drug needs to be evaluated through the drug use evaluation program (EPO) to ensure the rational and effective application of this drug. The purpose of this research is to examine the anti-ectoparasitic drugs that are often used and its effectivity in cases of infections caused by ectoparasites, in this study that is infection caused by Ctenocephalides felis fleas which cause the Flea Allergic Dermatitis and Sarcoptes scabiei mites which cause Scabies in cats and dog. This is a descriptive study using medical records from 127 patients who have infections caused by ectoparasites. The evaluation of this drug use is done descriptively by comparing data results and literatures. This study showed the use of anti-ectoparasitic drugs in animals with Flea Allergic Dermatitis in 20 times applications; 15 times using Imidacloprid 10% combined with Moxidectin 2.5% and 5 times using anti-ectoparasit ivermectin, while in animals with scabies the drug are used 107 times; 76 times using anti-ectoparasitic ivermectin, 26 times using Permethrin 5% and 5 times using Imidacloprid 10% combined with Moxidectin 2.5%. Imidacloprid 10% combined with Moxidectin 2.5% is the most used anti-ectoparasitic drugs to treat cases of Flea Allergic Dermatitis, while ivermectin is most used to treat cases of Scabies. Keywords: anti ectoparasite, evaluation of drug use, ivermectin, Imidacloprid 10% and Moxidectin 2.5%