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All Journal BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Jurnal Natur Indonesia Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Penbios: Jurnal Pendidikan Biologi dan Sains International Journal of Research in Community Services Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science AGRIC Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Rejeki Siti Ferniah

Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University

Author-ID : 265914

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Aktivitas Enzimatis Biakan Kapang Aspergillus Section Nigri DUCC (Diponegoro University Culture Collection) Dan Identifikasi Molekuler Isolat Potensial Sabrini, Zalia; Rukmi, Isworo; Ferniah, Rejeki Siti
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.1-5

The enzyme amylase, cellulase and protease are an extracellular enzymes are many produced by microorganisms such as fungi Aspergillus section Nigri. This research aims to find out the potential biakanes of Aspergillus section Nigri DUCC collection (Diponegoro University Culture Collection) which is capable to produce some enzymes that consists of amylase, protease and celullase and also identification in molecular based. Activity enzymatic assay of Aspergillus section Nigri withÂ semi-quantitative method using selective medium, CMC for cellulolytic, Starch agar 1% for amylolytic and Skim Milk agar for proteolytic. Biakane potential is determined by looking at the Enzymatic Index (EI) is highest for all of three enzyme. Molecular identification is using the universal primer ITS4 and ITS5. The results showed that the culture of DUCC K207Â has high activity for all of three enzyme. Index enzymatic of isolate DUCC K207 , 1.55 mm for amylolytic, 1.49 mm for cellulolytic and 1, 24 mm for proteolytic. Result of molecular identification DUCC K207Â known as Aspergillus niger that has 100% similarity with Aspergillus niger MH 109325.1.

ISOLASI KHAMIR DARI BATANG TANAMAN TEBU DAN IDENTIFIKASINYA BERDASARKAN SEKUENS INTERNAL TRANSCRIBED SPACER Ika Anggraini; Rejeki Siti Ferniah; Endang Kusdiyantini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Isolation of Yeasts from Sugarcane Stems and Their Identification Based on Internal Transcribed Spacer SequencesÂ ABSTRACTFermentative yeasts used in food, health, and energy industries need to be explored to discover their potential. The purpose of this study was to obtain fermentative yeast isolates from sugarcane stems and subsequently to undertake morphological, biochemical, and molecular identification. The isolation of epiphytic and endophytic yeasts was carried out by spread plate method using sugarcane soak water and sugarcane juice on potato dextrose agar (PDA) and yeast-glucose-peptone (YGP) agar media. Morphological identification was based on macroscopic and microscopic observations. Biochemical identification was performed using carbohydrate fermentation and 50%-glucose media tests. Selected isolates were identified molecularly using Internal Transcribed Spacer (ITS). Seven yeast isolates were obtained, of which isolate Ed 1B was selected. Isolate ED 1B was of round colonies, creamy white colour, shiny, embossed, and wavy appearance, ovoid cell shape with a cell diameter of 4.74 Âµm. It had budding cells, was able to ferment glucose and sucrose (but not lactose), and grew on 50 %-glucose media. Results of BLAST showed that isolates Ed 1B had 99% homology with Kodamaea ohmeri.Keywords: isolation, ITS, molecular identification, Saccharum officinarum L., yeastÂ ABSTRAKKhamir fermentatif yang digunakan dalam industri pangan, kesehatan dan energi perlu dieksplorasi untuk mengetahui potensinya. Tujuan penelitian ini adalah untuk memperoleh isolat khamir fermentatif dari batang tebu dan untuk kemudian diidentifikasi secara morfologi, biokimia dan molekuler. Isolasi khamir epifit dan endofit dilakukan dengan metode cawan sebar dari air rendaman tebu dan jus tebu pada media potato dextrose agar (PDA) dan yeast-glucose-peptone (YGP). Identifikasi morfologi berdasarkan pengamatan makroskopis dan mikroskopis. Identifikasi biokimia menggunakan uji fermentasi karbohidrat dan uji media glukosa 50%. Isolat terpilih diidentifikasi molekuler menggunakan Internal Transcribed Spacer (ITS). Hasil isolasi memperoleh 7 isolat khamir. Satu isolat terpilih (Ed 1B) didapatkan dan memiliki ciri-ciri koloni bulat, putih krem, mengkilap, timbul, bergelombang, bentuk sel ovoid dengan diameter sel 4,74 Âµm, memiliki budding cell, mampu memfermentasi glukosa dan sukrosa, tidak memfermentasi laktosa, serta tumbuh pada media glukosa 50%. Hasil BLAST menunjukkan bahwa isolat Ed 1B memiliki homologi 99% dengan Kodamaea ohmeri.Kata Kunci: identifikasi molekuler, isolasi, ITS, khamir, Saccharum officinarum L.

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI PANGAN FERMENTASI CINCALOK SEBAGAI PENGHASIL GAMMA-AMINOBUTYRIC ACID Adhitya Naufal Pribadhi; Endang Kusdiyantini; Rejeki Siti Ferniah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Isolation and Characterization of Lactic Acid Bacteria from Fermented Food Cincalok as Producer of Gamma-Aminobutyric Acid Cincalok is a fermented food originating from West Kalimantan. This study aimed to obtain lactic acid bacterial isolates (LAB) capable of producing gamma-aminobutyric acid (GABA), to characterize the LAB isolates obtained, and to obtain GABA by the Thin Layer Chromatography (TLC) method. Bacterial growth and GABA production was carried out by adding 5% MSG and without MSG, and measured spectrophotometrically. In this study, 4 LAB bacterial isolates were obtained which were coded CIN-1, CIN-2, CIN-3, and CIN-4. GABA identification of all the LAB isolates using TLC Silica Gel 60 F254 with butanol: acetic acid: distilled water (5: 3: 2) as eluent yielded Rf 0.61 and Rf MSG 0.38. The highest growth was achieved by isolate CIN-3 with an absorbance of 1.488 (at 48 hour) in non-MSG medium, while the addition of 5% MSG resulted in an absorbance of 1.631 (at 42 hour). GABA production was achieved by isolate CIN-3 with 5% MSG treatment with a concentration of 201.472 mM and without MSG with a concentration of 171.195 mM. Cincalok merupakan pangan fermentasi yang berasal dari Kalimantan Barat. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) yang mampu menghasilkan gamma-aminobutyric acid (GABA), melakukan karakterisasi isolat BAL yang diperoleh dan dapat diperoleh GABA dengan metode Kromatografi Lapis Tipis (KLT). Penumbuhan bakteri dan produksi GABA dilakukan dengan penambahan MSG 5% dan tanpa MSG, dan diukur menggunakan spektrofotometer. Dalam penelitian ini diperoleh 4 isolat bakteri BAL yang diberi kode CIN-1, CIN-2, CIN-3, dan CIN-4. Identifikasi GABA dari semua isolat BAL tersebut menggunakan KLT Silica Gel 60 F254 dengan eluen butanol: asam asetat: aquades (5: 3: 2), menghasilkan Rf 0,61 dan Rf MSG 0,38. Pertumbuhan tertinggi terjadi pada isolat CIN-3 non MSG dengan absorbansi 1,488 (jam ke-48), sedangkan dengan penambahan MSG 5% menghasilkan absorbansi 1,631 (jam ke-42). Produksi GABA dicapai isolat CIN-3 dengan perlakuan MSG 5% dengan konsentrasi 201.472 mM dan tanpa MSG dengan konsentrasi 171,195 mM.

Identifikasi Spesies Mikroalga dari BBPBAP Jepara secara Morfologi dan Molekuler menggunakan 18S rDNA Elke Gildantia; Rejeki Siti Ferniah; Anto Budiharjo; Agung Suprihadi; Muhammad Zainuri; Hermin Pancasakti Kusumaningrum
Buletin Oseanografi Marina Vol 11, No 2 (2022): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v11i2.39703

Mikroalga merupakan organisme eukariot bersel satu yang habitatnya berada di perairan. Suatu spesies mikroalga koleksi kultur BBPBAP Jepara berpotensi menghasilkan astaxantin dalam jumlah tinggi. Namun, spesies ini belum di karakterisasi secara molekuler. Penelitian ini bertujuan untuk memperoleh karakter morfologi dan molekuler pada isolat mikroalga dari BBPBAP Jepara menggunakan 18S rDNA guna memastikan spesies untuk pengembangan potensinya. Tahapan penelitian yang dilakukan meliputi kultivasi mikroalga, pengamatan pola pertumbuhan, pengamatan morfologi, isolasi DNA, amplifikasi menggunakan marka 18S rDNA, elektroforesis, analisis data sekuens, dan filogenetik menggunakan aplikasi NJ Plot dan MEGA 7. Hasil karakterisasi secara morfologi sel isolat mikroalga BBPBAP Jepara memperlihatkan bentuk bulat, berwarna hijau dengan ukuran 4,5 μm, tidak memiliki flagela, dan motilitas yang pasif. Hasil karakterisasi molekuler menggunakan 18S rDNA dari isolat mikroalga BBPBAP memperlihatkan kemiripan tertinggi dengan Chlorella sorokiniana sebesar 99%. Microalgae are single-celled eukariyotic organisms whose habitat is in the waters. A species of microalgae from the Jepara BBPBAP culture collection was potential to produce high amounts of astaxantin. However, this species has not been detected molecularly previously. This study aimed to obtain the morphological and molecular characters of microalgae isolates from BBPBAP Jepara using 18S rDNA to ascertain the species and its potential development. The stages of the research carried out include; microalgae cultivation, growth pattern observation, morphological observation, DNA isolation, amplification using 18S rDNA markers, electrophoresis, sequence data analysis, and phylogenetic using NJ Plot and MEGA applications 7. Results of morphological characterization of the Jepara BBPBAP showed that cell of microalgae isolates had a round shape, green color with a size of 4.5μm, has no flagella and passive motility. The results of the molecular characterization using 18S rDNA showed that isolate BBPBAP had highest similarity with Chlorella sorokiniana about 99%.

KARAKTERISTIK MORFOLOGI, BIOKIMIA, DAN MOLEKULER ISOLAT KHAMIR IK-2 HASIL ISOLASI DARI JUS BUAH SIRSAK (Annona muricata L.) Vivi Suryaningsih; Rejeki Siti Ferniah; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.943 KB)

Yeast was found in foods that contain lots of sugar as fruits. Yeast utilizes simple sugars in food to gain energy. Yeast has a role in the natural fermentation of the fruit that resulting of decay, discoloration, and cause the fruit not durable. The purpose of this research were to isolate the yeast from the soursop fruit and to identify the yeast in morphology, biochemistry, and molecular test based on Internal Transcribed Spacer (ITS). Isolation of yeast was performed by growing on medium PDA and chloramphenicol. Identification was done through biochemical test by looking at the capabilities in the fermentation of carbohydrate and the abilities to grow on a medium to high osmotic pressure. Molecular identification was done using Internal Transcribed Spacer (ITS) region. The result showed that isolate IK-2 yeast from the soursop fruit juice had a distinctive form round to oval, prominent elevation, the colour creamy white, form a buds, able to ferment glucose and sucrose, but not able to ferment lactose, as well as being able to grow on media with glucose level 50 %. Molecular analysis of the ITS region using ITS1 and ITS4 primers, and phylogenetic analysis using Neighbor Joining. The result of the Basic Local Alignment Tools (BLAST) showed that the isolate had 95% homology with Candida tropicalis. Key Words : Yeast, Soursop (Annona muricata L.), Internal Transcribed Spacer (ITS)

KERAGAMAN SECARA MOLEKULER BAKTERI ASAM LAKTAT PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PERLAKUAN PAKAN HASIL FERMENTASI Chrysonilia crassa Husnul Khotimah; Siti Nur Jannah; Rejeki Siti Ferniah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.673 KB)

Lactic Acid Bacteria (LAB) are microflora in the digestive tract that has positive roles in poultry’s health. The amount and diversity of LAB in the gastrointestinal tract are influenced by several factors, one of them is by the feeding factor. The study aims to analyze the LAB diversity in ileum and cecum after feeding of fermented Chrysonilia crassa molecularly. Analysis of LAB spesies diversity is needed to provide a baseline profile of microbial community database on the illeum and cecum digestive tract of broiler chicken between control (commercial feed) and treatment (feed by Chrysonilia crassa fermentation) by the method of Terminal Restriction Fragment Length Polymorphism (T-RFLP). The calculated values are the number of phyilotypes, relative abundance, Shannon-Wiener (H') diversity index, evenness (E), and similarity. Group of LAB detected in the control were Lactobacillus delbrueckii (180 bp), Lactobacillus sp. (187 bp), Lactobacillus plantarum (572 bp), uncultured bacterium (87 bp) and unidentified (50 bp, 582 bp). The result of this study showed that by giving the fermented feed by Chrysonilia crassa had resulted in the decreasing of LAB diversity in ileum (0.66), cecum (0.48) compared with commercial feed (control) in ileum (0.84), cecum (1.05). Keywords: broiler chicken, cecum, ileum, LAB, T-RFLP

Ekspresi Gen Penyandi Peroksidase Cabai Merah (Capsicum Annuum L.) (Caper) sebagai Respons terhadap Fusarium Oxysporum Athried Elsima; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 8 No. 2 Juli 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.391 KB)

Cabai merah (Capsicum annuum L.) merupakan komoditas pertanian penting di Indonesia yang sering terkena serangan OPT ( organisme pengganggu tanaman) misalnya Fusarium oxysporum. Tanaman cabai merah memiliki sifat ketahanan kimiawi aktif yaitu mengekspresikan peroksidase sebagai respons terhadap infeksi patogen. Penelitian ini bertujuan untuk mengetahui ekspresi gen penyandi peroksidase pada tanaman cabai merah sebagai respons terhadap jamur F. oxysporum. Penelitian ini diawali dengan inokulasi jamur pada tanaman cabai merah dilakukan dengan metode rendaman akar. Analisis ekspresi gen penyandi peroksidase menggunakan qRT-PCR dilakukan pada gen CaPer dengan gen 18s rRNA sebagai gen pembaku, pada interval 6, 48, dan 96 jam setelah inokulasi. Hasil penelitian gen CaPer terekspresi paling tinggi pada 6 jam setelah inokulasi.

Pertumbuhan Daun Tanaman Cabai (Capsicum annuum L.) yang Diinfeksi Fusarium oxysporum pada Umur Tanaman yang Berbeda Himmatul Ulya; Sri Darmanti; Rejeki Siti Ferniah
Jurnal Akademika Biologi Vol. 9 No. 1 Januari 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.098 KB)

Cabai merah merupakan salah satu komoditas hortikultura yang ditanam di Indonesia. Salah satu penyakit yang menyerang tanaman cabai adalah layu fusarium. Layu fusarium diakibatkan oleh infeksi fungi Fusarium oxysporum. Infeksi F.oxysporum menyebabkan pengguguran daun lebih cepat sehingga menyebabkan penurunan daerah fotosintesis. Penelitian ini bertujuan untuk mengetahui pertumbuhan dan pengguguran daun tanaman cabai yang diinfeksi fungi F.oxysporum pada fase vegetatif dan generatif. Parameter yang diamati adalah jumlah daun yang dihitung setiap 5 hari, luas daun dihitung setiap 20 hari, dan jumlah daun gugur yang dihitung setiap 5 hari. Setiap perlakuan dilakukan 5 ulangan. Hasil yang didapatkan dari penelitian ini adalah tanaman cabai yang diinfeksi fungi F.oxysporum pada fase generatif mengalami penurunan pertumbuhan daun lebih tinggi dan pengguguran daun lebih banyak bila dibandingkan dengan tanaman cabai yang diinfeksi F.oxysporum pada fase vegetatif.

Karakter Rhizobakteri Pelarut Fosfat Potensial dari Rhizosfer Tumbuhan Mangrove Teluk Awur Kabupaten Jepara secara Mikrobiologi Eka Oktaviani; Arina Tri Lunggani; Rejeki Siti Ferniah
Jurnal Ilmu Lingkungan Vol 18, No 1 (2020): April 2020
Publisher : School of Postgraduate Studies, Diponegoro Univer

Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR adalah dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial dilakukan secara mikrobiologi dan atau uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter.

Bioprospecting of Bacillus pumilus as biocontrol against fungal plant pathogens Ade Fajrian; Rejeki Siti Ferniah; Anto Budiharjo
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The genus Bacillusis one of the wellknown group of bacteria as Plant Growth-Promoting Rhizobacteria (PGPR) that can support plant growth or otherwise control the plant pathogens. In this study, three strain of B. pumilusfrom BIOTEC culture collection (BCC 7507, TBRC 2887 and TBRC 2903) were investigated for their potential to be used as fungal pathogen control. The growth characteristics of these B. pumilusstrains were determined by making the growth curve of each strain. Further, antagonistic activity of B. pumilusagainst the anthracnose pathogens C. capsici, C. gloeosporioidesand C. acutatum, the rice dirty panicle pathogen Curvularia lunata, the leaf spot pathogen Alternaria brassicicola, and the rice blast pathogen Magnoporthe griseawere evaluated by identified the inhibition zone from their activity. Then, the secondary metabolites produced by these Bacillusstrains were also examined using anti-fungal activity assays. All B. pumilusstrains showed very low biocontrol activity against M. grisea, but not against C. acutatum, C. Capsici, C. gloeosporioides, A. brassicicolaand C. lunata. Culture extracts derived from these strains did not show any activity against M. griseain the spore germination inhibition assay. These results showed that the three B. pumilusstrains did not exhibit strong potential for application as biocontrol agent.

Co-Authors Achmadi Priyatmojo Ade Fajrian Adhitya Naufal Pribadhi Agung Suprihadi Akbar, Muhammad Falih Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anto Budiharjo Arina Tri Lunggani Arina Tri Lunggani Athried Elsima Budi - Raharjo BUDI SETIADI DARYONO Candra Wahyuningsih Chiesa Salsabila Dewi, Yulita Wiwik Irana Diana Ayu Fitriana Dinda Khairunnisa, Dinda Eka Oktaviani Eko Purnomo Elke Gildantia Elsima, Athried Endang Kusdiyantini Endang Kusdiyantini Erfianti, Tia Fauzaan, Muhammad Faishal Furgeva, Natasha Halwiyah, Nurul Hermin Pancasakti Kusumaningrum Himmatul Ulya Himmatul Ulya Himmatul Ulya HUSNUL KHOTIMAH Ika Anggraini Ika Anggraini Is Helianti IS HELIANTI Istiana, Rohma Jennefer Constantia Jumari Jumari, Jumari K, Hermin Pancasakti Khoirin Nida Kurniawati, Mufida Budi Lina Mulyawati Mawarni, Shelina Nurunnisa MG Isworo Rukmi Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Rahmandanni, Yunnia Rina Sari Asih Rina Sri Kasiamdari Rizko, Nurmalisa Sabrini, Zalia salamah salamah Siti Nur Jannah Siti Nur Jannah Sri Darmanti Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sunarno s Supriyadi Supriyadi Suryaningsih, Vivi Susiana - Purwantisari Susiana Purwantisari Tri Rahayu, Hesti Vivi Suryaningsih Weni Lestari, Weni Wijanarka Wijanarka Yumna Rahmadias Hanifa Yunnia Rahmandanni

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