Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.286

P-Index

This Author published in this journals

All Journal BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Jurnal Natur Indonesia Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Penbios: Jurnal Pendidikan Biologi dan Sains International Journal of Research in Community Services Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science AGRIC Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Rejeki Siti Ferniah

Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University

Author-ID : 265914

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

Published : 48 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

The expression of glucanase encoding gene (CaβGlu)in chili (Capsicum annuum L.) as a response to Fusarium oxysporum infection Yunnia Rahmandanni; Sri Pujiyanto; Rejeki Siti Ferniah
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Indonesia is a tropical country with highest level of biodiversity, especially in the agricultural sector. Chili (Capsicum annumm. L) is a very well-known and widely used agricultural product in the world, which makes chili becomes one of the most considerable national product. The chili production is oftentimes very susceptible to some diseases caused by virus, fungi, or bacteria. One of the most common diseases in chili cultivation is Fusarium wilt, which is caused by Fusarium oxysporum. This disease can cause a major loss and up to 50% crop failure. Many procedures have been done to find the best cultivar with a resistance trait to Fusarium oxysporum, including by observing and testing the chili’s genetic resistance. One of the resistance genes in chili is β -1, 3- glucanase-encoding gene, which produces an enzyme to hydrolize the cell wall of pathogenic fungi. This research aimed to determine the expression of the glucanase-encoding gene (CaβGlu) in chili as a response to Fusarium oxysporum infection. The methods including chili cultivation, F. oxysporum inoculation, isolation of chili leaves RNA, glucanase-encoding gene expression analysis used qRT-PCR, and data analysis. The result of CaβGlu gene expression is higher than the control in the first 6 hours after inoculation, and decreasing in the 48th and 96th hours. The conclusion was the infection of Fusarium oxysporum is activating the expression of CaβGlu gene which was expressed best in the first 6 hours after inoculation.

Indonesian red chilli (Capsicum annuum L.) capsaicin and its correlation with their responses to pathogenic Fusarium oxysporum Rejeki Siti Ferniah; Sri Pujiyanto; Hermin Pancasakti Kusumaningrum
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Red chili is a commercial crop for the food industry in Indonesia. There are some categories of red chili based on their pungency. The hot chili usually has more capsaicin than the sweet chili. Some cultivars may have more resistance to pathogen infection than the others. This research aimed to analyze the disease resistance of red chili cultivars from Indonesia against pathogenic Fusariumoxysporum and the correlation with capsaicin contents. Disease resistance was examined by determination of the Disease Severity Index (DSI) 15 dpi (days post inoculation). The correlation was analyzed by the regression coefficient. The result showed that the most resistance cultivar against F. oxysporum was Branang, while Lembang-1displayed the contrary. There was not a correlation of capsaicin content with the chili resistance to F. oxysporum.

Respons Fisiologi Tanaman Cabai (Capsicum annuum) Var. Lembang 1 Terhadap Infeksi Fusarium oxysporum pada Umur Tanaman yang Berbeda Himmatul Ulya; Rejeki Siti Ferniah; Sri Darmanti
Buletin Anatomi dan Fisiologi Volume 5, Nomor 2, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.2.2020.174-182

Cabai merah merupakan komoditas hortikultura yang banyak dibudidayakan oleh petani, namun penyakit layu fusarium yang disebabkan oleh infeksi fungi Fusarium oxysporum menjadi kendala utama dalam budidaya. Penelitian ini bertujuan untuk mengkaji respons fisiologi tanaman cabai kultivar Lembang-1 pada umur tanaman yang berbeda terhadap infeksi F.oxysporum. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x2. Faktor pertama adalah infeksi F.oxysporum (diinfeksi dan tidak diinfeksi) dan faktor kedua adalah umur tanaman cabai saat infeksi (35 dan 75 hari setelah tanam). Masing-masing perlakuan dengan 3 ulangan. Parameter yang diamati adalah panjang dan lebar porus stomata, kadar pigmen fotosintesis dan pertumbuhan relative tanaman cabai. Data kuantitatif yang diperoleh dianalisis dengan Analysis of Variance (Anova) dilanjutkan dengan uji DMRT pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa tanaman cabai yang diinfeksi F.oxysporum pada umur 35 SHT lebih responsif dibanding dengan yang diinfeksi pada umur 75 SHT. Respon fisiologinya tanaman cabai yang diinfeksi pada hari ke 35 setelah tanam adalah penyempitan lebar porus stomata sebesar 31,2%, penurunan kadar pigmen klorofil dan karotenoid sebesar 18%, penurunan tinggi batang 10,4% dan penurunan panjang akar 15,9%.Kata Kunci: defisiensi air, infeksi fungi, stomata, xylem

Semi-Manual Processing Of Blood Clamps Waste into Chitosan Powder Chiesa Salsabila; Candra Wahyuningsih; Diana Ayu Fitriana; Rina Sari Asih; Khoirin Nida; Rejeki Siti Ferniah
International Journal of Research in Community Services Vol 3, No 1 (2022)
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijrcs.v3i1.185

Blood clams (Anadara granosa) are endemic clams found in Southeast Asia and East Asia. Blood clams are widely consumed by the public as seafood dishes in coastal food stalls. The great potential of blood clams will increase the waste of clam shells produced. The accumulation of shellfish waste will cause pollution and reduce environmental aesthetics. The chitin content in blood clam shells can be used as chitosan. Chitosan is a polymer of -(1-4) glucosamine which is formed when the acetyl group in chitin is substituted by hydrogen to become an amine group. Chitosan has antibacterial and antifungal properties. Isolation of chitosan was carried out through the stages of demineralization, deproteination, and deacetylation. The limited use of laboratories during the pandemic is a major obstacle in the isolation process of chitosan. This study aims to process blood clam shell waste into chitosan in a simple way on a home scale. Processing includes deproteination, demineralization, and deacetylation were done using tools and materials available at home. Laboratory equipment such as beakers could be replaced with pots, the reflux process was replaced by using a cloth to filter, and measuring cups were replaced with glasses. The research used 1500 grams of blood clam shell powder and produced 1050 grams of white chitosan with a slightly hard texture

IDENTIFIKASI MOLEKULER JERUK NIPIS TEGAL BERDASARKAN FRAGMEN GEN 18S RIBOSOMAL RNA Yumna Rahmadias Hanifa; Sri Pujiyanto; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Tegal’s lime is a lime that grows in the coastal areas with high salinity, and research on the molecular identification of Tegal’s lime has never been done before. This study aims to determine the molecular identity and its phylogenetic relationship with other oranges in GenBank. DNA isolation was carried out using CTAB. The best DNA purity was 1.883 while the highest DNA concentration was 464.83 ng/?L. DNA amplification was carried out using the PCR method which consisted of denaturation, annealing, and extension steps. DNA was electrophoresed with agarose gel. The DNA band size was 572 bp. The fraqment of 18S rRNA of Tegal’s lime has a greatest similarity of 99,64% with Citrus sinensis, while based on phylogenetic tree it has a closest relationship with C. aurantium (bootstrap value 33%). The alignment of Tegal’s lime against the C. aurantium sample showed that there were 2 gaps and 4 base changes, while the alignment with other oranges and C. medica as an ancestral showed that there were transition and transversion at the 130th and 560th nucleotide respectively. Jeruk nipis Tegal merupakan jeruk nipis yang tumbuh pada daerah pesisir yang memiliki salinitas cukup tinggi dan penelitian tentang identitas jeruk nipis Tegal masih belum pernah dilakukan sebelumnya. Oleh karena itu, penelitian ini bertujuan untuk mengetahui identitas jeruk nipis Tegal secara molekuler dan mengetahui hubungan kekerabatannya dengan jeruk lainnya pada GenBank. Isolasi DNA dilakukan dengan menggunakan CTAB. Kemurnian DNA terbaik yaitu 1,883 sedangkan konsentrasi DNA tertinggi adalah 464,83 ng/?L. Amplifikasi DNA dilakukan dengan menggunakan metode PCR yang terdiri dari tahap denaturasi, anealing, dan ekstensi. DNA dianalisis menggunakan elektroforesis gel agarosa. Ukuran pita DNA yang didapatkan yaitu 572 pb. Fragmen gen 18S rRNA jeruk nipis Tegal memiliki kesamaan yang paling tinggi dengan Citrus sinensis sebesar 99,64% sedangkan berdasarkan analisis filogenetik, memiliki hubungan kekerabatan yang paling dekat dengan C. aurantium (nilai bootstrap sebesar 33%). Hasil pensejajaran jeruk nipis Tegal terhadap sampel C. aurantium menunjukkan adanya 2 gap dan 4 perubahan basa sedangkan pensejajaran yang dilakukan antara jeruk nipis Tegal dengan jeruk lain dan jeruk nenek moyang C. medica menunjukkan adanya 1 transisi pada basa ke-130 dan 1 transversi pada basa ke-560.

Molecular Characterization of Rhizobacteria (Isolate EO-4) as Potential Solvent of Phosphate in-vitro from the Mangrove Ecosystem of Teluk Awur, Jepara-Indonesia Eka Oktaviani; Rejeki Siti Ferniah; Arina Tri Lunggani
PENBIOS: JURNAL PENDIDIKAN BIOLOGI DAN SAINS Vol 5 No 01 (2020): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Vol. 5, No. 1, Mei 2020 pISSN 2541
Publisher : Biology Education Department, Faculty of Teacher Training and Education, University of Nahdlatul Wathan Mataram - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/penbios.v5i01.207

Rejuvenation and conservation of mangrove ecosystems, especially the Teluk Awur mangrove ecosystem, Jepara Regency, can be done by using bacteria that are capable of supporting plant growth or called Plant Growth Promoting Rhizobacteria (PGPR). One of the mechanisms that support plant growth by the PGPR group is phosphate dissolving activity, because the phosphate in the soil is in the form of a compound that is difficult for plants to absorb. This study aims to determine the character of Rhizobacter isolates as a superior phosphate solvent in dissolving phosphates in-vitro from the Teluk Awur mangrove ecosystem, namely isolates coded EO-4. These isolates have similar microbiological and biochemical characters to the genus Enterobacter. Molecular characterization of isolates was carried out using the Polymerase Chain Reaction (PCR) method - 16S rRNA sequence analysis (comparing with 16S rRNA sequences in gene banks). The results showed that the phosphate-solubilizing rhizobacteria that were isolated had the same base pair percentage of 48% with Enterobacter pyrinus (access number NR_028875).

Pelatihan Pembuatan VCO Menggunakan Isolat Bakteri Asam Laktat Asal Usus Ayam dan Pemanfaatan Limbahnya Menjadi Pupuk Cair di Kelurahan Jerakah Semarang Siti Nur Jannah; Hermin Pancasakti Kusumaningrum; Rejeki Siti Ferniah; Sri Pujiyanto
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Semarang dengan berbagai kekayaan Sumber Daya Hayati (SDH) yang sangat melimpah, terutama dalam hal ini kelapa belum dimanfaatkan dan diolah secara optimal, sehingga kontribusinya dalam peningkatan ekonomi masyarakat masih belum dirasakan. Berdasarkan latar belakang tersebut, kami akan mengadakan kegiatan pengabdian kepada masyarakat melalui penerapan IPTEKS yang sudah kami lakukan dalam skala laboratorium, berupa diversifikasi produk olahan berbasis santan kelapa yang dapat digunakan untuk membuat VCO (Virgin Coconut Oil). Tujuan program pengabdian masyarakat adalah mengaplikasikan teknologi fermentasi oleh bakteri asamlaktat menggunakan santan kelapa untuk pembuatan VCO dan pupuk cair di kelompok PKK dan guru-guru di Madrasah Ibtidaiyyah Walisongo Jerakah Semarang. Metodologi yang digunakan adalah penyuluhan dan praktek tentang pembuatan VCO dan penanganan limbahnya menjadi pupuk cair. Hasil dari Pengabdian ini adalah produk, metode/teknologi tepat guna, dan publikasi. Diharapkan pembuatan produk VCO dan pupuk cair yang dihasilkan memberi manfaat pengetahuan tentang fermentasi dan dapat digunakan sebagai usaha inisiasi bisnis bagi kelompok PKK dan guru di Jerakah Semarang.

Cloning of a Gene Encoding Protease from Bacillus halodurans CM1 into Escherichia coli DH5α and Expression Analyses of the Gene Product Helianti, Is; Furgeva, Natasha; Mulyawati, Lina; Ferniah, Rejeki Siti; Kusumaningrum, Hermin Pancasakti
Makara Journal of Science Vol. 22, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Bacillus halodurans strain CM1 is an Indonesia alkalothermophilic bacterium isolated from Cimanggu Hot Spring, Bandung, West Java. This bacterial strain produces high levels of thermoalkalophilic xylanase. It has also been predicted to produce other potential industrial enzymes, including protease. For production and application of protease in the future, the protease gene from B. halodurans CM1 was cloned into Escherichia coli. The protease gene was isolated from B. halodurans CM1 by the PCR approach using primers designed based on the GenBank. The PCR product was then ligated into pGEM-T Easy vector, transformed into E. coli DH5α, verified, and analyzed based on DNA sequencing data using the BLAST search tool. A 1086-bp protease gene was obtained that exhibited a very high sequence similarity (99%) with that of alkaline protease gene from B. halodurans C-125. When the culture of this positive recombinant E. coli DH5α containing the protease gene was spotted onto calcium caseinate agar, a clear zone appeared after incubation at 50 °C. This result demonstrated that the protease gene was expressed in this recombinant E. coli DH5α.

Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified Â morphologically and molecularly as F. oxysporum. Â

Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Rejeki Siti Ferniah; Endang Kusdiyantini; Nurhayati Nurhayati
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p57-62

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.

Co-Authors Achmadi Priyatmojo Ade Fajrian Adhitya Naufal Pribadhi Agung Suprihadi Akbar, Muhammad Falih Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anto Budiharjo Arina Tri Lunggani Arina Tri Lunggani Athried Elsima Budi - Raharjo BUDI SETIADI DARYONO Candra Wahyuningsih Chiesa Salsabila Dewi, Yulita Wiwik Irana Diana Ayu Fitriana Dinda Khairunnisa, Dinda Eka Oktaviani Eko Purnomo Elke Gildantia Elsima, Athried Endang Kusdiyantini Endang Kusdiyantini Erfianti, Tia Fauzaan, Muhammad Faishal Furgeva, Natasha Halwiyah, Nurul Hermin Pancasakti Kusumaningrum Himmatul Ulya Himmatul Ulya Himmatul Ulya HUSNUL KHOTIMAH Ika Anggraini Ika Anggraini Is Helianti IS HELIANTI Istiana, Rohma Jennefer Constantia Jumari Jumari, Jumari K, Hermin Pancasakti Khoirin Nida Kurniawati, Mufida Budi Lina Mulyawati Mawarni, Shelina Nurunnisa MG Isworo Rukmi Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Rahmandanni, Yunnia Rina Sari Asih Rina Sri Kasiamdari Rizko, Nurmalisa Sabrini, Zalia salamah salamah Siti Nur Jannah Siti Nur Jannah Sri Darmanti Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sunarno s Supriyadi Supriyadi Suryaningsih, Vivi Susiana - Purwantisari Susiana Purwantisari Tri Rahayu, Hesti Vivi Suryaningsih Weni Lestari, Weni Wijanarka Wijanarka Yumna Rahmadias Hanifa Yunnia Rahmandanni

Title

Found 48 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 48 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 48 Documents
Search