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Journal : Bioscience

Isolation of Phosphate Solubilizing Endophytic Fungi From Rice Plant Root Dezi Handayani; Mades Fifendy; Verawati Yesni
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.472 KB) | DOI: 10.24036/020182110043-0-00

Abstract

Root endophytic fungi plays different roles for plant, such as plant growth promoting properties, agents to control phytopathogens, and increase phosphorus uptake. Since phosphorus are essential for plant growth and its occurance are limited, so it is necessary to explore these fungus to replace the used of synthetic fertilizer. The objective of this study were to obtain root endophytic fungi from rice plant and to determine its phosphate solubilization ability. The root organ of rice plant was subjected for isolation. Pikovskaya medium was use to determine the fungal phosphorus solubilization ability. Fungal morphological characteristics was carried out by macroscopic and microscopic appearance assessment using microscope. Seven endophytic fungi were successfully isolated from rice plant root sample. Four isolate were micelial steril with no conidia, two isolate refers to Aspergillus and 1 isolate have 2-4 conidia at the tip of conidiophores. Amongs 7 endophytics fungi, only one isolate (P2B3) had the ability to solubilize phosphate with the phosphate solubilization index value 20.45 %.
Effect of Time Incubation To Quality and Organoleptics Soygurt Mades Fifendy; Syukryani Syukryani
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.431 KB) | DOI: 10.24036/0201932104571-0-00

Abstract

Abstract. Soy is a soursce of vegetable protein that is recommended for consumption. One of the soybean ingredients known to the general public is soy milk. Soy milk is a health drink that has nutritional value equivalent to cow's milk. Utilization of soy milk is still limited because the flavor is less favored. To get a good taste can be processed through the process of fermentation by utilizing the bacteria Streptococcus thermophillus and Lactobacillus bulgaricus which will produce Soygurt. How long is the proper incubation time to produce soygurt at room temperature is unknown. The purpose of this research is to know the effect of incubation time on quality and organoleptic value of soygurt. This research is an experimental research with Completely Random Design 8 treatment, 3 replications. The treatments consisted of different incubation periods: 0 hours (control), 10 hours, 12.5 hours, 15 hours, 17.5 hours, 20 hours, 22.5 hours, 25 hours. Parameters measured were acidity, fat content, protein content and organoleptic value. The acidity data was analyzed by Anava. The results showed that the treatment given significant effect on acidity level. Levels of acidity, fat content, and protein content meet the Indonesian National Standard. For oerganoleptic test the highest score for color was on the 17,5 hour treatment and the viscosity was at 22,5 hours and 25 hours treatment. While for the favorite test is the treatment of 10 hours, 12.5 hours, 17.5 hours and 20 hours. The incubation time to affects the quality and value of the organoleptic soygurt. 
Ecological Preference of Soil Texture to Distribution of Mangrove Seedling Species in the Forest Mangrove Teluk Buo, Padang-Sumatera Barat irma - leilani; Hendra Lardiman; Mades Fifendy
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.155 KB) | DOI: 10.24036/020182110042-0-00

Abstract

A research has been conducted to find out the ecological preferences of soil texture on seedling of mangrove species in mangrove forest of Teluk Buo, Padang of West Sumatra. The study was done from May to July 2008. Samples were taken by making 3 transect lines perpendicular to the coastline. 26 observation plot 10 x 10 m was placed continuously zigz zag left to right along the transect line. In each plot is recorded the species of seedling were found and soil texture. Soil texture analized in the lab. Biology FMIPA-UNP. The study revealed that there is an ecological freferences the soil texture to the distribution of seeding of mangrove species. The seedling of Rhizophora apiculata, Ceriop tagal and Sonneratia alba was collected in areas with dusty clay soil texture. The seedling of Avicennia marina was collected on sandy loam soil and sand. The seedling of Bruguiera gymnorhyza only present on the clay sandy soil.
Isolasi dan Identifikasi Bakteri Halofilik Ikan Talang (Chorinemus sp.) dari Aia Bangih Pasaman Barat Mades Fifendy; Faradilla Rattriana; Irdawati Irdawati
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.828 KB) | DOI: 10.24036/02017128075-0-00

Abstract

Halophilic bacteria is microorganism that live in high salinity environments up to 30%. The bacteria can be found in foods preserved by salting like salted fish. Salted fish is preserved foods are processed by salting and drying. The bacteria that live and ruin salted fish are halophilic bacteria and heterotoleran bacteria like halobacterium, micrococcus, bacillus, pediococcus, peseudomonas and vibrio. This observation aims to determine type of halophilic bacteria found in talang fish.  This is descriptif observation held january up to february 2015 is microbiology laboratory FMIPA UNP and balai veteriner laboratory Bukittinggi. Research methods is cup statterplot methods. Sample is talang fis from Aia Bangih, west pasaman. Based on observation found isolate halophilic bacteria. Its belong to gram positve like coccus. From both, the isolates not capable produce endosopra. Talang fish from Aia Bangih, west pasaman contain two isolate halophilic bacteria consist of a genus and two species.Keywords : talang salted fish, halphilic bacteria, biochemical test
The influence of carbon sources on the production of siderophores from the fluorescent pseudomonad bacteria Ilham Pratama; Linda Advinda; Mades Fifendy
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.573 KB) | DOI: 10.24036/020182210406-0-00

Abstract

Abstract.Pseudomonad fluorescent is one of the rhizobacteria groups that could potentially be developed as a crop endurance inducer. Several species of fluorescent pseudomonad are able to produce siderophores. Siderophore is an antimicrobial organic compound that plays a role in biological control of plant diseases. This study aims to determine the best carbon source for the production of siderophores from the fluorescent pseudomonad isolates PfCas3 and PfLAHp2. The carbon sources are fructose, glucose, and glycerol. Detection of siderophores was measured using a spectrophotometer at a wavelength of 410 nm. The results showed that the best growth medium for producing siderophores was KB + glucose medium for both PfCas3 and PfLAHp2 isolates. The best combination was the use of PfCas3 isolate with the addition of carbon glucose source which resulted in the production of siderophores of 1.574. Keywords: Fluorescent pseudomonad, carbon sources, siderophore
Making Pond And Feed Of Catfish By Farmer’s Wife At Nagari Limau Gadang Armen Amir; Mades Fifendy; Ristiono Ristiono
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/02017128080-0-00

Abstract

The life of Nagari Limau Gadang residents is poor. They conduct economic activities in Kerinci Seblat National Park (TNKS), so that TNKS become damaged. To reduce the economic activities of the people in TNKS, it is necessary to conduct economic activities outside TNKS to improve the family economy. Catfish farming can serve as the main effort to increase family income. Economically catfish farming is very profitable. Catfish farming is not difficult and can be done by farmers who do not usually cultivate freshwater fish. The right family member is empowered to improve the family economy through the cultivation of catfish is the farmer's wife. Farmer’s wives in Limau Gadang Nagari tend not to improve the family economy, they play the role of taking care of children, doing housework and sometimes delivering food to the fields and to the fields. The farmer’s wife activity is only fulfilling the wife's obligation to husband in married life. Farmers' wives are economically unproductive in improving the family economy. The goal to be achieved in this research is for the wife of farmers can contribute to improve the family economy. Specific targets to be achieved in this study is the wife of skilled farmers cultivate catfish, Nagari Limau Gadang population increases their living standards and TNKS in Nagari Limau Gadang free from the pressure of the population. How to research, selected 20 poor farmer's wife then trained to make ponds and pellets. The pools made are the main pond, spawning pond, nursery pool and pond enlargement. Pellet made from raw trash fish, quail feces, fine bran and cassava. The pellet formulation is made of 4 kinds. Each formulation produces a quality pellet. Based on laboratory test produced pellet contains very good nutrition for enlargement catfish. The nutritional range of protein is 21,79%-34,60%, fat is 1,95%-2,32% and carbohydrate is 26,55%-39,65%. The farmer's wife understands and skillfully creates ponds and pellets. Pellet made from raw materials trash fish, quail feces, fine bran and cassava can be used as raw material pellet. Keyword: cultivation, catfish, farmer's wife, income, family.