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Synergystic Role of Cocopeat Matrix and Foliar Nutrient Type on Aclimatization Success of Micropropagated Dendrobium Seedlings Baharuddin Mohammad Luthfi; Ramdan Hidayat; Pangesti Nugrahani
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8174

Abstract

Background: Dendrobium orchids have high economic value (in 2020 generated an export value of US$61.87 thousand), yet micropropagated seedlings commonly exhibit slow growth and high mortality during the acclimatization stage. This problem is largely associated with the use of unsuitable planting media and inadequate nutrient supply, which limit seedling adaptation and survival after tissue culture. This study aims to determine the best combination of planting media and foliar fertilizer types for the growth of Dendrobium orchid seedlings during the acclimatization stage. Methodology: The study was conducted in Penambangan Village, Semanding District, Tuban Regency, East Java Province from November 2024 to February 2025 using a Completely Randomized Design (CRD) with 9 treatment combinations and 4 replications. The first factor was the treatment of planting media types (M1: Black Moss, M2: Rice Husk Charcoal, M3: Cocopeat), and the second factor was the type of foliar fertilizer (P1: Gandasil D, P2: Gaviota 63, P3: Grow Quick LB). The observed research variables included the percentage of live seedlings, plant height, leaf area, seedling growth rate, number of shoots, and number of roots. Findings: The results showed that the use of cocopeat planting media resulted in the highest percentage of seedling survival at 88.33%. The combination of cocopeat planting media and Grow Quick LB leaf fertilizer produced the best relative growth rate (RGR) at 8-12 WAP of 41.22 mg/month.This research produces the best combination of planting media and foliar fertilizer for orchid seedlings at the acclimatization stage. Contribution: The findings contribute to a more systematic understanding of how substrate physical properties and foliar nutrient supply interact to influence survival and growth performance during orchid acclimatization, offering a scientifically grounded framework for improving ex vitro propagation efficiency
Growth Performance and Yield of Lettuce (Lactuca sativa L.) on Different Media in an NFT Hydroponic System Youmandja Arif Riyak Thiombiano; Pangesti Nugrahani; Ramdan Hidayat
Journal of Tropical Crop Science Vol. 13 No. 02 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.02.370-381

Abstract

The operational success of closed-loop hydroponic systems is largely governed by the synergistic selection of the cultivation substrate and crop variety. An optimal growing medium must provide reliable structural stability, uniform moisture retention, and localized nutrient accessibility, which, when coupled with a cultivar’s inherent genetic potential, dictate overall vegetative development and marketable yield. This study evaluated the effects of different growing media and lettuce cultivars on growth dynamics and yield performance in a nutrient film technique (NFT) system. The experiment was arranged in a two-factor completely randomized design, evaluating lettuce cultivars ‘Arroyo’, ‘Amaroza’, and ‘Espirando’ and growing substrates: rockwool, cocopeat, and peatmoss. The results revealed significant interaction effects between cultivar and growing media for plant height at 28 days after transplanting (DAT) and rosette canopy diameter at 7 DAT. The treatment combination integrating the cultivar ‘Arroyo’ with a peat moss substrate consistently yielded the highest values for these growth parameters. Furthermore, across all evaluated growing media, ‘Arroyo’ demonstrated superior biomass accumulation and overall productivity, highlighting its robust agronomic suitability for tropical NFT hydroponic cultivation.
Composition of Growing Media and Concentration of Foliar Fertilizer Affected Yield and Quality of Purple Eggplant (Solanum melongena L.) Ramdan Hidayat; Melinda Dwi Safitri; Pangesti Nugrahani
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.48

Abstract

The study aimed to obtain the best combination of growing media composition and the concentration of foliar fertilizer (Gandasil B) on the yield quality of eggplant. The research was conducted from December 2022 to February 2023 in Nganjuk Regency, using a completely randomized design of two factors. The first factor was composition of the growing media, which comprised of soil:husk charcoal:cow manure with ratios of (1:1:1), (1:1:2), (1:2:1), and (2:1:1), and the second factor was the concentration of foliar fertilizer (0, 2, 4, 6 g/L). The results exhibited that the combination of (1:2:1) medium growing composition and the 2 g/L concentration of foliar fertilizer produced the best fruit diameter with an increase of 22.35% compared to the combination of growing media (1:1:1) and concentration of foliar fertilizer of 2 g/L. The composition of the soil growing medium 1:2:1 gave the best results on fruit length and diameter, average fruit weight, and total fruit weight per plant. A quadratic regression analysis of the concentration of foliar fertilizer and the number of purple eggplant fruits showed that the highest concentration of foliar fertilizer was 5.78 g/L. Keywords: foliar fertilizer, fruit diameter, growing medium, purple eggplant
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Damasa Ines Larrisa; Pangesti Nugrahani; Sri Wiyatiningsih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Damasa Ines Larrisa; Pangesti Nugrahani; Sri Wiyatiningsih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
Cauliflower (Larissa F1) Response to Media Composition and Liquid Organic Fertilizer under Limited Land Condition in Bogem, Sidoarjo An Nisa Eka Pratiwi; Pangesti Nugrahani; Agus Sulistyono
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i3.7792

Abstract

Background : Cauliflower (Brassica oleracea L.) is a highly valued horticultural vegetable comodity. Previous research on the application of Jimmy Hantu LOF on cauliflower has focused on the polybag planting system with a single type of media, whereas this study focuses on the variation in planting medium composition (soil, goat dung, and burnt husks) in conjunction with LOF concentration. This method is expected to provide more thorough information regarding the interaction between growing media and liquid organic fertilizer as a strategy to boost cauliflower productivity in limited land settings. Methodology: The research was carried out at Sidoarjo, East Java. Polybags with various liquid organic fertilizer compositions and dosages were used in this research. The study used a completely randomized factorial design with two treatment factors: the kind of planting media (three treatment levels) and the concentration of liquid fertilizer (four treatment levels). Findings: There was a significant interaction between the soil composition treatment goat manure: burnt husks (1:1:1) (M2) and the content of liquid organic fertilizer 14 ml/L (P3) when compared to the control treatment. The treatment resulted in a 34.42 % difference in the height of cauliflower plants and a 44.93 % difference in the number of leaves. The results show that combination of planting medium soil: goat manure: burnt husk (1:1:1) (M2) with the application of 14 ml/L of Jimmy Hantu liquid organic fertilizer is the most successful treatment. Contribution: These findings provide scientific evidence on the synergistic effect of planting media composition and liquid organic fertilizer concentration, offering a valuable reference for optimizing sustainable B. oleracea cultivation in limited land conditions
Flowering response of torch ginger (Etlingera elatior) to PK fertilization RAMDAN HIDAYAT; PANGESTI NUGRAHANI; RAHMATUN NISFUL MAGHFIROH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261230

Abstract

Abstract. Hidayat R, Nugrahani P, Maghfiroh RN. 2025. Flowering response of torch ginger (Etlingera elatior) to PK fertilization. Biodiversitas 26: 6305-6315. Demand for the flowers of Etlingera elatior has increased, but predictable and timely flowering is constrained by its naturally long vegetative phase. This research aimed to determine the combined effect of dosage and frequency of PK compound fertilizer in inducing flowering in torch ginger. The field trial was carried out at Dapoer Ketjombrang Garden, Giripurno Village, Bumiaji District, Batu City, East Java, Indonesia, from December 2023 to April 2024. A factorial experiment was conducted using a randomized complete block design (two factors: dosage (D): 50, 100, 150, 200 g/clump; frequency (F): 1, 2, 3 applications at 30, 30+45, and 30+45+60 DAT, respectively) with three replicates (36 plots, 3 plants/plot, total 108 plants) was used. Data were analyzed using a two-way ANOVA (dosage, frequency, dosage×frequency), and means were compared using Tukey’s HSD (α: 0.05). The combination D4F2 (200 g, 2x at 30 and 45 DAT) produced the earliest flowering (mean 21.89 DAT). The dosage D3 (150 g) produced the best vegetative growth and, when combined with F2, gave the highest number of flowers (23.78 flowers/clump). Dosage effects were significant (p<0.05) for several traits; frequency had limited main effects, but dosage×frequency interactions were observed. The highest C:N ratio, antioxidant level, and vitamin C were observed at D4; regression between C:N and flowering age showed R²: 0.92 (p: 0.041). Overall, applying 150-200 g PK fertilizer per clump in two applications (30 and 45 DAT) provides an effective strategy to induce earlier flowering and increase flower productivity under the conditions tested. Multi-season and multi-site validation is recommended before making broad recommendations.
The Effect of Zn Nanoparticle Concentration and the Comparison of Growth Regulator Concentration on the Growth of Cavendish Banana Explants (Musa acuminata) Sarah Nurmala Putri Rukmana; Pangesti Nugrahani; Didik Utomo Pribadi
Edu Spectrum: Journal of Multidimensional Education Vol. 1 No. 2 (2024): Edu Spectrum: Journal of Multidimensional Education
Publisher : Pusat Riset dan Inovasi Nasional Mabadi Iqtishad Al Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70063/eduspectrum.v1i2.46

Abstract

Cavendish banana (Musa acuminata L.) propagation through conventional methods requires a considerable amount of time. Tissue culture techniques offer a more efficient alternative, enabling the production of plantlets with superior quality and quantity in a shorter period. The application of Zn nanoparticles has been shown to reduce media contamination and act as a biostimulant, enhancing propagation efficiency in tissue culture. Auxins, such as Naphthalene Acetic Acid (NAA), facilitate cell development and root induction, while cytokinins, including 6-Benzyl Amino Purine (BAP), are effective in stimulating cell division, morphogenesis, and shoot formation with improved stability and resistance to oxidation. This study was conducted from February to June 2023 at the Biotechnology Laboratory, Faculty of Agriculture, Universitas Pembangunan Nasional "Veteran" Jawa Timur. The research utilized a Completely Randomized Design (CRD) with two factors: the concentration of Zn nanoparticles and the ratio of ZPT concentrations. The results indicated that the best treatment for multiplication was obtained with 50 ppm Zn nanoparticles, resulting in the fastest shoot emergence at 24,63 days and the highest number of shoots per plantlet at 2,58 shoots. Furthermore, the treatment with a ZPT concentration ratio of NAA + BAP (0:0) produced the highest average number of plantlet leaves, with 5,78 leaves per plantlet.