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Uji Aktivitas Diuretik Ekstrak Etanol Kombinasi Daun Putri Malu (Mimosa pudica L.) dan Daun Pacar Kuku (Lawsonia inermis L.) pada Tikus Jantan Galur Wistar Dwi Novita Eka Sari; Fransiska Leviana; Kisrini Kisrini
Jurnal Farmasi Indonesia Vol 11 No 2 (2014): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Putri malu (Mimosa pudica L.) and pacar kuku (Laswonia inermis L.) leaves can be used as diuretic agent. This research aimed to find out the diuretic effect of combination of putri malu and pacar kuku leaves ethanol extract as the effective dose on Wistar male rat. The tested animal used consisted of 35 rats, divided into 7 groups: furosemida positive control (1,44 mg/200 g BW), negative control CMC 0.5%, extract of putri malu leaves (20 mg/200 g BW), extract of pacar kuku leaves (50 mg/200 g BW), combination of putri malu and pacar kuku leaves ethanol extract (10 : 25 mg/200 g BW; 5 : 37,5 mg/200 g BW; 15 : 12,5 mg/200 g BW). Observation was conducted every hour for 6 hours, and then at 12 and 24 hours. The diuretic effect was obtained by estimating cumulative urine volume and AUC (Area Under The Curve). The data was processed using oneway variance analysis followed by Tukey HSD test. The result of research the combination of putri malu and pacar kuku leaves ethanol extract has a diuretic effect. The extract combination had diuretic effect higher than the single extract. The combination of putri malu and pacar kuku leaves ethanol extract dose of 5 mg/200 g BW : 37,5 mg/200 g BW and 15 mg/200 g BW : 12,5 mg/200 g BB had diuretic activity compatible to the positive control.

Penetapan Kadar Fenolik Total pada Fraksi n-Heksana, Etil Asetat, Air dan Ekstrak Daun Bayam Merah (Amarantus gangeticus Hort) dengan Metode Folin-Ciocalteu FITRI PUJIATI; FRANSISKA LEVIANA
Jurnal Farmasi Indonesia Vol 9 No 1 (2012): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Red spinach leaves (Amarantus gangeticus Hort) is one of plant that contain phenolic compound which has benefit for human. The aim of this study was to determine total phenolic content on n-hexane, ethyl acetate, and water fractions of ethanolic extract of red spinach by Folin-Ciocalteu. Red spinach leaves were macerated by ethanol 70%, than were fractionated by of n-hexane, ethyl acetate, and water. Extract and fractions were analyzed the total phenolic content by Folin-Ciocalteu method. The study showed that total phenolic content of red spinach were (4,05%±0,175) GAE for ethanolic extract; (2,15%±0,045) GAE for n-hexane fraction, (11,03%±0,290) GAE for ethyl acetate fraction; (4,4%±0,180) GAE for water fraction.

Optimasi Carbopol 940 dan Gliserin dalam Formula Gel Lendir Bekicot (Achatina fulica Ferr) sebagai Antibakteri Staphylococcus aureus dengan Metode Simplex Lattice Design Arsiaty Sumule; Ilham Kuncahyo; Fransiska Leviana
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 17 No. 01 Juli 2020
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v17i1.5640

Lendir bekicot (Achatina fulica Ferr) mengandung protein achasin, suatu senyawa dengan aktivitas antibakteri yang mendukung proses penyembuhan luka. Selain itu, peptida mytimacin-AF pada lender bekicot diketahui menghambat pertumbuhan Staphylococcus aureus ATCC 25923. Lendir bekicot kurang praktis jika digunakan secara langsung sehingga perlu dikembangkan menjadi bentuk sediaan gel. Penelitian ini bertujuan untuk mengetahui proporsi optimum campuran carbopol 940 dengan gliserin dalam formula gel dan mengevaluasi aktivitas antibakteri formula optimum terhadap S. aureus ATCC 25923. Gel lendir bekicot dibuat tiga formula dengan komposisi carbopol 940 dan gliserin masing-masing sebesar 1; 1,5; dan 2%, serta 15; 14,5; dan 14%. Semua formula diuji sifat fisiknya selama empat minggu, meliputi organoleptis, homogenitas, viskositas, daya sebar, daya lekat, dan pergeseran viskositas. Hasil uji dioptimasi dengan metode simplex lattice design menggunakan program Design Expert 8.0.6.1. Formula optimum yang diperoleh dievaluasi sifat fisiknya selama empat minggu, sedangkan aktivitas antibakterinya diuji dengan menggunakan metode difusi sumuran. Hasil penelitian menunjukkan formula optimum gel lendir bekicot dengan proporsi campuran carbopol 940 dan gliserin masing-masing 1,123 dan 14,877%. Formula optimum memiliki aktivitas antibakteri terhadap S. aureus dengan diameter zona hambat sebesar 1,73 cm.

UJI ANTIBAKTERI EKSTRAK DAN FRAKSI HERBA CEPLUKAN (Physalis angulata L.) UNTUK MENGATASI INFEKSI Staphylococcus epidermidis SELAMA PERSALINAN Renata Tri Anggreany; Ismi Rahmawati; Fransiska Leviana
DINAMIKA KESEHATAN: JURNAL KEBIDANAN DAN KEPERAWATAN Vol 11, No 1 (2020): Dinamika Kesehatan Jurnal Kebidanan dan Keperawatan
Publisher : Universitas Sari Mulia

Latar Belakang: Herba ceplukan (Physalis angulata L.) merupakan salah satu dari tanaman obat yang mengadung beberapa senyawa aktif yang diduga bersifat antibakteri. Kehamilan dengan ancaman persalinan preterm dikarenakan pertumbuhan kuman Staphylococcus epidermidis.Tujuan: dari penelitian ini adalah mengetahui aktivitas antibakteri ekstrak etanol, fraksi n-heksan, etil asetat, dan air herba ceplukan terhadap bakteri Staphylococcus epidermidis.Metode: Penelitian eksperimental dengan melakukan penyarian herba ceplukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian difraksinasi dengan pelarut n-heksan, etil asetat, dan air. Hasil ekstrak dan fraksinasi diuji aktivitas antibakteri menggunakan metode difusi dengan konsentrasi 20%; 10% dan 5%. HasilÂ fraksi teraktif dilanjutkan uji makrodilusi. Identifikasi kandungan kimia herba ceplukan dikerjakan dengan menggunakan uji tabung dan Kromatorafi Lapis Tipis (KLT).Hasil: Penelitian menghasilkan rendemen ekstrak, fraksi n-heksan, fraksi etil asetat dan fraksi air berturutan sebesar 21,88; 10,90; 3,96 dan 52,00%. Hasil uji aktivitas antibakteriÂ menunjukkan fraksi n-heksan, etil asetat danÂ air memiliki aktivitas antibakteri terhadap Staphylococcus epidermidis. Fraksi etil asetat merupakan fraksi teraktif dibandingkan fraksi yang lain dengan diameter daya hambat terbesar pada konsentrasi 20% sebesar 23,33 mm. Fraksi etil asetat mengandung senyawa flavonoid, alkaloid, saponin, dan terpenoid yangÂ bertanggung jawab terhadap aktivitas antibakteri. Hasil penelitian dengan metode dilusi menunjukkan bahwa Konsentrasi Bunuh Minimum fraksi etil asetat adalah 5%.Â Kata kunci: herba ceplukan (Physalis angulata L.), fraksinasi, Staphylococcus epidermidis, antibakteriÂ AbstractBackground: Cutleaf groundcherry herb (Physalis angulata L.) is one of the medicinal plants that contains several active compounds that are suspected to be antibacterial. Pregnancy with preterm labor risk caused by the growth of Staphylococcus epidermidis.Aim: This study was conducted to Â determine the antibacterial activity of ethanol extract, fraction of n-hexane, ethyl acetate, and cutleaf groundcherry herb water against Staphylococcus epidermidis.Method: The extraction of cutleaf groundcherry herb was carried out by maceration method using 70% ethanol, then fractionated with n-hexane, ethyl acetate, and water. The extract and fractionation results were tested for antibacterial activity using a diffusion method with a concentration of 20%; 10% and 5%. Identification of the chemical content of the herbal cep need is done by using tube test and Thin Layer Chromataphy (TLC).Result: The study produced extract yield, n-hexane fraction, ethyl acetate fraction and water fraction of 21.88; 10,90; 3.96 and 52.00%, respectively. Antibacterial activity test results showed that the n-hexane, ethyl acetate and water fractions had antibacterial activity against Staphylococcus epidermidis. Ethyl acetate fraction was the most active fraction compared to other fractions with the largest inhibitory diameter of 23.33 mm at a concentration of 20%. The ethyl acetate fraction contained flavonoids, alkaloids, saponins, and terpenoids which are responsible for antibacterial activity. The results of the study using the dilution method showed that the Minimum Killing Concentration of ethyl acetate fraction was 5%.Â Key Words: Cutleaf groundcherry herb (Physalis angulata L.), fractionation, Staphylococcus epidermidis, antibacterial.

UJI ANTIBAKTERI EKSTRAK DAN FRAKSI HERBA CEPLUKAN (Physalis angulata L.) UNTUK MENGATASI INFEKSI Staphylococcus epidermidis SELAMA PERSALINAN Renata Tri Anggreany; Ismi Rahmawati; Fransiska Leviana
DINAMIKA KESEHATAN: JURNAL KEBIDANAN DAN KEPERAWATAN Vol 11, No 1 (2020): Dinamika Kesehatan Jurnal Kebidanan dan Keperawatan
Publisher : Universitas Sari Mulia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33859/dksm.v11i1.560

Latar Belakang: Herba ceplukan (Physalis angulata L.) merupakan salah satu dari tanaman obat yang mengadung beberapa senyawa aktif yang diduga bersifat antibakteri. Kehamilan dengan ancaman persalinan preterm dikarenakan pertumbuhan kuman Staphylococcus epidermidis.Tujuan: dari penelitian ini adalah mengetahui aktivitas antibakteri ekstrak etanol, fraksi n-heksan, etil asetat, dan air herba ceplukan terhadap bakteri Staphylococcus epidermidis.Metode: Penelitian eksperimental dengan melakukan penyarian herba ceplukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian difraksinasi dengan pelarut n-heksan, etil asetat, dan air. Hasil ekstrak dan fraksinasi diuji aktivitas antibakteri menggunakan metode difusi dengan konsentrasi 20%; 10% dan 5%. HasilÂ fraksi teraktif dilanjutkan uji makrodilusi. Identifikasi kandungan kimia herba ceplukan dikerjakan dengan menggunakan uji tabung dan Kromatorafi Lapis Tipis (KLT).Hasil: Penelitian menghasilkan rendemen ekstrak, fraksi n-heksan, fraksi etil asetat dan fraksi air berturutan sebesar 21,88; 10,90; 3,96 dan 52,00%. Hasil uji aktivitas antibakteriÂ menunjukkan fraksi n-heksan, etil asetat danÂ air memiliki aktivitas antibakteri terhadap Staphylococcus epidermidis. Fraksi etil asetat merupakan fraksi teraktif dibandingkan fraksi yang lain dengan diameter daya hambat terbesar pada konsentrasi 20% sebesar 23,33 mm. Fraksi etil asetat mengandung senyawa flavonoid, alkaloid, saponin, dan terpenoid yangÂ bertanggung jawab terhadap aktivitas antibakteri. Hasil penelitian dengan metode dilusi menunjukkan bahwa Konsentrasi Bunuh Minimum fraksi etil asetat adalah 5%.Â Kata kunci: herba ceplukan (Physalis angulata L.), fraksinasi, Staphylococcus epidermidis, antibakteriÂ AbstractBackground: Cutleaf groundcherry herb (Physalis angulata L.) is one of the medicinal plants that contains several active compounds that are suspected to be antibacterial. Pregnancy with preterm labor risk caused by the growth of Staphylococcus epidermidis.Aim: This study was conducted to Â determine the antibacterial activity of ethanol extract, fraction of n-hexane, ethyl acetate, and cutleaf groundcherry herb water against Staphylococcus epidermidis.Method: The extraction of cutleaf groundcherry herb was carried out by maceration method using 70% ethanol, then fractionated with n-hexane, ethyl acetate, and water. The extract and fractionation results were tested for antibacterial activity using a diffusion method with a concentration of 20%; 10% and 5%. Identification of the chemical content of the herbal cep need is done by using tube test and Thin Layer Chromataphy (TLC).Result: The study produced extract yield, n-hexane fraction, ethyl acetate fraction and water fraction of 21.88; 10,90; 3.96 and 52.00%, respectively. Antibacterial activity test results showed that the n-hexane, ethyl acetate and water fractions had antibacterial activity against Staphylococcus epidermidis. Ethyl acetate fraction was the most active fraction compared to other fractions with the largest inhibitory diameter of 23.33 mm at a concentration of 20%. The ethyl acetate fraction contained flavonoids, alkaloids, saponins, and terpenoids which are responsible for antibacterial activity. The results of the study using the dilution method showed that the Minimum Killing Concentration of ethyl acetate fraction was 5%.Â Key Words: Cutleaf groundcherry herb (Physalis angulata L.), fractionation, Staphylococcus epidermidis, antibacterial.

Antibacterial Activity of Karanda (Carissa Carandas) Leaf and Fruit Extract to Against Streptococcus mutans, Escherichia coli, Staphylococcus aureus Destik wulandari; Mamik Ponco Rahayu; Fransiska Leviana
Jurnal Kesehatan Farmasi Vol 4, No 1 (2022)
Publisher : Jurusan Farmasi, Poltekkes Kemenkes Palembang

Tanaman karanda (Carissa carandas) memiliki aktivitas antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak daun dan buah karandas sebagai antibakteri terhadap Streptoccocus mutans, Escherichia coli, Staphylococcus aureus. Daun dan buah karandas diekstraksi menggunakan metode maserasi dengan pelarut methanol. Ekstrak daun dan buah karanda diuji aktivitas antibakterinya menggunakan metode difusi seumuran. Hasil penelitian menunjukkan bahwa ekstrak daun Carissa carandas mampu menghambat bakteri S. aureus dan E. coli dan ekstrak buah mampu menghambat bakteri S. mutans dengan optimal.

PERBANDINGAN AKTIVITAS ANTIOKSIDAN, KADAR FENOL TOTAL, DAN KADAR FLAVONOID TOTAL EKSTRAK DAUN DURIAN (Durio zibethinus L.) MUDA DAN TUA Fransiska Leviana; Luvi Nurharisna; Zani Mariastuti; Hally Fazah Iyah
MEDFARM: Jurnal Farmasi dan Kesehatan Vol 12 No 1 (2023): MEDFARM: Jurnal Farmasi dan Kesehatan
Publisher : LPPM Akafarma Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.48191/medfarm.v12i1.169

Daun durian mengandung senyawa fenolik yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan ekstrak daun muda dan tua durian dan membandingkan kadar fenol total dan flavonoid total ekstrak daun muda dan tua durian. Ekstraksi daun durian muda dan tua dilakukan secara maserasi dengan pelarut etanol 70%. Ekstrak daun durian diuji aktivitas antioksidannya terhadap radikal DPPH dengan mengukur absorbansi dengan spektrofotometer UV-Vis, kemudian dihitung persen peredaman dan nilai IC50. Rutin digunakan sebagai kontrol positif. Penetapan kadar fenol total dengan metode Folin-Ciocalteu dan kadar flavonoid total dengan pereaksi aluminium klorida menggunakan spektrofotometri UV-Vis. Data IC50 dan kadar total fenol daun tua dan daun muda dianalisis menggunakan independent sample t-test. Hasil penelitian menunjukkan bahwa aktivitas antioksidan ekstrak etanol daun durian tua lebih tinggi dibandingkan daun muda. Hal ini disebabkan kadar fenol total dan flavonoid total daun durian tua signifikan lebih tinggi dibandingkan daun muda.

Potensi Ekstrak Kasar Enzim dari Tempe Kedelai Hitam (Glycine soja (L.) Merr.) sebagai Obat Fibrinolitik Alami dengan Metode Clot Lysis In Vitro: Potential of Crude Enzymes from Black Soybean Tempeh (Glycine soja (L.) Merr.) as a Natural Fibrinolytic Medicine with Clot Lysis In Vitro Method Nadea Sherly Widya Putri Nadea; Ana Indrayati; Fransiska Leviana
Jurnal Sains dan Kesehatan Vol. 5 No. 2 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i2.1712

Fibrinolytic enzymes are enzymes that can be used to break down fibrin. In thrombus therapy (cardiovascular disease), this enzyme has an activity similar to plasmin, which is able to reduce fibrin by hydrolyzing fibrin into dissolved products and inhibiting the formation of fibrin clots that can trigger cardiovascular disease. Black soybean tempeh has various benefits, one of which is to lower the risk of cardiovascular disease and reduce the risk of blood clots. Due to the content of the enzyme protease in black soybeans which is able to degrade fibrin threads. The purpose of this study is to determine the potential of black soybean tempeh (Glycine soja (L.) Merr) can produce enzyme extracts as natural fibrinolytic drugs. This study was conducted by taking ingredients, crude extraction of enzymes from black soybean tempeh by centrifugation, purification of crude extracts of enzymes using ammonium sulfate salts of 80%, determination of enzyme protein levels with the Lowry method and testing of fibrinolytic potential with the clot lysis method in vitro. The variation in the concentration of the extract used in the test was 12.5; 25; 50 and 100%. The results showed that crude extracts of black soybean tempeh enzymes were able to lyse blood clots. The protein content of the black soybean tempeh extract sample after purification using ammonium sulfate was 245.76 μg/mL. The optimum percentage of blood clot lysis at a concentration of 100% of the sample after purification was 59%. Keywords: Black soybean, fibrinolytics, fibrin, ammonium sulfate, clot lysis Abstrak Enzim fibrinolitik adalah enzim yang dapat digunakan untuk memecah fibrin. Pada terapi trombus (penyakit kardiovaskular), enzim ini memiliki aktivitas yang mirip dengan plasmin yaitu mampu menurunkan fibrin dengan menghidrolisis fibrin menjadi produk terlarut dan menghambat pembentukan bekuan fibrin yang dapat memicu adanya penyakit kardiovaskular. Tempe kedelai hitam memiliki berbagai manfaat salah satunya adalah menurunkan risiko penyakit kardiovaskular dan mengurangi risiko penggumpalan darah. Karena kandungan enzim protease pada kedelai hitam yang mampu mendegradasi benang fibrin. Tujuan dari penelitian ini untuk mengetahui potensi tempe kedelai hitam (Glycine soja (L.) Merr) dapat menghasilkan ekstrak enzim sebagai obat fibrinolitik alami. Penelitian ini dilakukan dengan pengambilan bahan, ekstraksi kasar enzim dari tempe kedelai hitam dengan sentrifugasi, pemurnian ekstrak kasar enzim menggunakan garam ammonium sulfat 80%, penetapan kadar protein enzim dengan metode Lowry serta pengujian potensi fibrinolitik dengan metode clot lysis secara in vitro. Variasi konsentrasi ekstrak yang digunakan dalam pengujian adalah 12,5 ; 25 ; 50 dan 100%. Hasil penelitian menunjukkan ekstrak kasar enzim tempe kedelai hitam mampu melisiskan bekuan darah. Kadar protein sampel ekstrak tempe kedelai hitam setelah dilakukan pemurnian menggunakan ammonium sulfat sebesar 245,76 μg/mL. Persentase lisis bekuan darah yang optimum pada konsentrasi 100% dari sampel yang setelah dilakukan pemurnian yaitu sebesar 59%. Kata Kunci: Kedelai hitam, fibrinolitik, fibrin, ammonium sulfat, clot lysis

Pengujian Sifat Anti-Inflamasi Ekstrak Bunga Chamomile dalam Nanopartikel Kristal Cair Lyotropik Secara In Vitro Widyan Muchzadi Akbar; Muhammad Dzakwan; Fransiska Leviana
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 12 No. 1 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/farmasains.v12i1.17402

Chamomile is renowned for its anti-inflammatory properties. Lyotropic Liquid Crystal Nanoparticle (LLCN) offers better enhancement of the bioavailability and efficacy of natural products. This study evaluated the LLCN system’s effectiveness in improving chamomile extract’s anti-inflammatory activity and compliance with nanoparticle standards. Chamomile 70% ethanol extract was prepared, and the total phenolic and flavonoid content was measured. The extract was formulated into LLCN, and the particle size, polydispersity index, zeta potential, stability, and entrapment efficiency were evaluated. Anti-inflammatory activity was tested in vitro using the protein denaturation inhibition method. The extract showed high phenolic and flavonoid content. The characterisation results demonstrated that the LLCN system met the required specifications, exhibiting particle size values of 230.366±3.412 nm, polydispersity index of 0.217±0.034, a zeta potential of -23.763±0.756 mV, and an entrapment efficiency of 84.060%±0.100. Stability testing indicated that storage at 4°C was more effective than room temperature, preserving a smaller particle size and improving overall stability. The LLCN system significantly enhanced the extract’s anti-inflammatory activity, as demonstrated by a lower IC50 value than the viscous extract. In conclusion, the LLCN system enhances the therapeutic potential of chamomile extract and represents a promising strategy for developing phytochemical-based anti-inflammatory formulations.

In Vitro Analysis of Anti-Inflammatory Activity of Lyotropic Liquid Crystal Nanoparticles from Echinacea purpurea Flower Extract Santianingtyas, Priscilla Sherly Ayu; Dzakwan, Muhammad; Leviana, Fransiska
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 2 (2024): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i2.555

Inflammation serves as the body's defense mechanism in response to tissue damage caused by various factors. Echinacea contains flavonoids such as quercetin, kaempferol, and diosmetin, which exhibit significant anti-inflammatory properties by inhibiting inflammatory enzymes and reducing the production of proinflammatory mediators. This study aims to evaluate the anti-inflammatory effects of echinacea flower extract formulated as Lyotropic Liquid Crystal Nanoparticles (LLCN) and compare its efficacy with viscous echinacea flower extract and diclofenac sodium. Echinacea flower extract was produced using 70% ethanol solvent via ultrasonication. LLCNs were synthesized using a top-down approach and characterized based on particle size, zeta potential, stability, and absorption efficiency. Anti-inflammatory activity was assessed in vitro using 0.2% Bovine Serum Albumin (BSA) solution in Tris Buffer Saline (TBS), with diclofenac sodium as a positive control. The results showed that echinacea flower extract can be converted into LLCN with particle sizes ranging from 10 to 1000 nm. The LLCN formulation of echinacea flower extract showed stronger anti-inflammatory activity compared to the condensed extract, with activity recorded at 54,660 ppm, IC50 for LLCN at 21,823 ppm, and IC50 for diclofenac sodium at 19,984 ppm. Thus, echinacea flower extract LLCN has potential as a natural anti inflammatory agent, which shows higher efficacy compared to its condensed extract form. This indicates that LLCN technology not only improves the bioavailability, but also the effectiveness of the extract in inhibiting inflammation, thus LLCN has the potential to be applied more widely in the development of natural drugs for anti inflammatory therapy.

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