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Journal : Bioplante

ISOLASI, IDENTIFIKASI DAN PEMURNIAN CENDAWAN MIKORIZA ARBUSKULAR (CMA) DARI TANAH BEKAS TAMBANG BATU BARA (Isolation, Identification and Purification of Arbuscular Mycorrhiza Fungi (AMF) from Coal Post Mining Soil) Elis Kartika; . Lizawati; . Hamzah
Bioplantae Vol. 1 No. 4 (2012): Bioplantae
Publisher : Bioplantae

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Abstract

Land of coal post-mining is the critical area that generally can not becultivated due to very low levels of fertility of the land, so this landbecomes slighted. One of the alternatives to overcome this problem isthrough inoculation with Arbuscular Mycorrhiza Fungi (AMF).Indigenous AMF (from coal post-mining location) is more potential AMFdeveloping in that area. Therefore, isolation, identification and purificationsteps of AMF spores are required. The objective of this study was toisolate, identify, and purify of arbuscular mycorrhiza fungi from coal postmining area. The study had identified that at this soil was found 3 AMFgenuses, i.e. Glomus, Acaulospora, and Gigaspora. On coal post-miningsoil was found 20 strains of AMF (13 strains of Glomus, 3 strains ofAcaulospora, and 1 strain of Gigaspora). In this soil was dominated byGlomus. Strain’s AMF that was successful isolated from single sporeculture was 4 strains i.e. Glomus sp-3, Glomus sp-6, Glomus sp-15, danGlomus sp-16.
PROLIFERASI KALUS DAN EMBRIOGENESIS SOMATIK JARAK PAGAR (JATROPHA CURCAS L.) DENGAN BERBAGAI KOMBINASI ZPT DAN ASAM AMINO (Callii Proliferation and Somatic Embryogenesis of Physic Nut (Jatropha curcas L.) Various Combination with PGR’s and Amino Acids) . Lizawati
Bioplantae Vol. 1 No. 4 (2012): Bioplantae
Publisher : Bioplantae

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Abstract

The aim of this study was to reveal a combination of plant growthregulators (PGR’s) and amino acids best callii proliferation and somaticembryogenesis physic nut. The experiment was arranged in completelyrandomized design with combination of plant growth regulators (PGR’s)and amino acids, i.e. 1 ppm TDZ + 1 ppm 2,4-D + 100 ppm glutamine;2 ppm BAP + 1 ppm 2,4 - D + 100 ppm glutamine; 1 ppm TDZ + 1 ppm2,4-D + 100 ppm CH (Casein hydrolyzate); 2 ppm BAP + 1 ppm 2,4-D+ 100 ppm CH (casein hydrolyzate); 1 ppm TDZ + 1 ppm 2,4-D; 2 ppmBAP + 1 ppm 2,4-D; Mannitol 3%; proline 5,75 ppm; proline 2.875ppm. The parameters observed were callii weight, callii diameter, calliicharacteristics (colour and structure) and the number of embryogeniccallii. The result showed that some treatment combinations of plantgrowth regulators and amino acids can increase the callii weight andcallii diameter. The color of celli was dominated by green and creamwith mostly compact structure. Meanwhile, celli friable structure as intreatment 1 ppm TDZ + 1 ppm 2,4-D; proline 5.75 ppm and 2.875 ppm.Key words : casein hydrolyzate, BAP, glutamine, proline, TDZ
INDUKSI KALUS EKSPLAN DAUN DURIAN (Durio zibethinus Murr. cv. Selat Jambi) PADA BEBERAPA KOMBINASI 2,4-D DAN BAP (Callus Induction Explants Leaf Durian (Durio zibethinus Murr. cv. Selat Jambi) With 2,4-D And Bap Combination) . Lizawati; . Neliyati; Retna Desfira
Bioplantae Vol. 1 No. 1 (2012): Bioplantae
Publisher : Bioplantae

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Abstract

The aim of this study was to obtain the best combination of 2,4-D and BAP in inducing callus from leaf explants durian cv. Selat Jambi. Experiment was arranged in completely randomized design with a growth regulators combination of treatment 2,4-D (1, 2, 3, 4, 5, 6, 7 ppm) and BAP (0,0 ; 0.5 ppm). Each treatment consisted of 10 bottles each culture bottle were planted on explant culture. Explants were cultured on induction medium for 2 month. The parameter time of calli initiation were observed every day. Meanwhile the percentage of explant forming callus, callus structure and color of callus was observed at the end of the study. The results showed that, granting some combination of growth regulators 2,4-D and BAP are given to the culture medium was able to stimulate the formation of callus on leaf explants young durian CV. Selat Jambi. Time of the fastest callus initiation (8 days after cultur) was obtained on medium 4 ppm 2,4-D + 0.5 ppm BAP. In contrast, the highest percentage of explants forming callus was obtained on treatment of 5 ppm 2,4-D (30%).Key words: Exsplants, Durio zibethinus, callus, BAP, 2,4-D
INDUKSI KALUS EMBRIOGENIK DARI EKSPLAN TUNAS APIKAL TANAMAN JARAK PAGAR (Jatropha curcas L.) DENGAN PENGGUNAAN 2,4 D DAN TDZ Lizawati .
Bioplantae Vol. 1 No. 2 (2012): Bioplantae
Publisher : Bioplantae

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Abstract

This studyaims at determine the interactionbetween the different level of 2.4-D andTDZ concentrations, to induction theembryogeniccallus of physic nut. This Research used completely randomized design with factorial treatment pattern, which consists oftwofactors :concentration of2,4 –D, which consists of 5levels : 0.0, 2.5,  5.0,  7.5, 10.0 ppm, and the concentration ofTDZconsists of 5levels: 0.0,  0.5,  1.0, 1.5, 2.0 ppm. The results showes that there are interactions between 2,4-D andTDZ to  formingcallus   ofthe apicalbudexplants of physic nut.The combination of5.0 ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZproducedthe time appearing of callusfastestcompared toother  treatments. The highestpercentage ofcallus formation occuredin thetreatment of2.5ppm2,4-D + 1.0ppmTDZand7.5ppm2,4-D + 1.5ppmTDZ. Whiteyellow color, shinyand friable are characteristicof embryogenic callusproduced on thetreatment of0.0 ppmand 2.5ppm2,4-D with the addition ofTDZata concentration of 0.5ppm, 1.0 ppm, 1.5 ppmand 2.0ppm.   Key words : embryogenic, callus, TDZ
FENOLOGI PERTUMBUHAN VEGETATIF DAN GENERATIF TANAMAN DUKU VARIETAS KUMPEH PADA BERBAGAI UMUR (The Phenology of Vegetative and Generative Growth of Duku Kumpeh Varieties at Various Age) . Lizawati; Budiyati Ichwan; . Gusniwati; . Neliyati; Muhammad Zuhdi
Bioplantae Vol. 2 No. 1 (2013)
Publisher : Bioplantae

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The objective pf this research was to determine the phenology of vegetative and generative growth of duku plant variety kumpeh at different ages. The treatment was design in randomized block design (RBD) with duku plant age duku as a treatment. The treatments tested were analysis : LA=Age plants less than 25 years; plants LB=Age 25-50 years, and LC=Age 50 years and older plants. On the obtained results, that theair temperature has a positive correlation to the length of shoots, shoots diameter and number of leaves at different plant age duku. Poster for the end of all plant life duku happened in September but there is a difference between the starting time and peak Poster. Flowering in plants duku occurredin the village of Karang Cityin early Octoberis the end of the dry season or the beginning of the rainy season. Duku fruit that reaches fruit set can beharvestedat 125 days after anthesis. Duku plants aged >50 produce fruit that tastes sweeter, with asugarcontent of 18.93% and 0.36% acid levels. Key words :Anthesis, Lansium domesticum, trubus