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THE GROWTH AND ANATOMICAL RESPONSE OF BLACK POTATO (PLECTRANTHUS ROTUNDIFOLIUS) TO FERMENTED COW, GOAT AND CHICKEN MANURE IN THE LOWLANDS Ngadiani; Andriani, Vivin
Lux Mensana: Journal of Scientific Health Vol. 2 Issue 2 (2023)
Publisher : jfpublisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56943/jsh.v2i2.289

Introduction. Food independence in Indonesia continues to be developed with other food crops to substitute the rice, one of it is by black potato. The productivity of lowland potato plants can be supported by the provision of nutrients. This research aims to determine the provision of fermented cow, goat and chicken manure on the growth, yield and anatomy of Black Potato Tubers (Plectranthus rotundifolius). Method. The method used was experimental with 12 treatments and repeated 3 times for each treatment, including control, 0 kg/plot, 5 kg/plot, 10 kg/plot, 15 kg/plot, and NPK. The parameters observed in this research were growth (plant height, number of leaves, number of branches) and anatomy (diameter of stems and stomatal density). Results and Analysis. The data were analyzed using Anova test with a significance of α = 0.05, and the difference test is conducted using Duncan when there is a difference in the test result. Discussion. The results showed that chicken manure had a better effect on the growth and anatomy of black potato plants compared to goat manure and cow manure.

PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITY OF LOWLAND BLACK POTATO PEELS (PLECTRANTHUS ROTUNDIFOLIUS) WITH COW MANURE USING ULTRASONIC BATH EXTRACTION METHOD Ngadiani, Ngadiani; Andriani, Vivin; Binawati, Diah Karunia
Lux Mensana: Journal of Scientific Health Vol. 2 Issue 4 (2023)
Publisher : jfpublisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56943/jsh.v2i4.459

Introduction. Black potato (Plectranthus rotundifolius) is one of the tubers in Indonesia and not well known by the public. It causes inadequate use of black potato than any other tubers. The parts that are currently used are the potato flesh. Meanwhile, the black potato peels are not used. Although the black potato peels contain polyphenol saponins, flavonoids, and antioxidant compounds. Method. Phytochemicals can be separated using extraction methods, one of the modern extraction methods used is the ultrasonic bath method. The ultrasonic bath method is a method that uses ultrasonic waves with a frequency greater than 16-20 KHz. The objective of this research is to determine the phytochemical content and antioxidant activity of lowland black potato peels with cow manure using ultrasonic bath extraction method. The research was conducted experimentally using RAL (Completely Randomized Design) with 3 factors and 3 replications, which included the difference in extraction temperature (55°C); extraction time (15 and 20 minutes); and the ratio of materials and solvents (1:5 and 1:10). Results & Analysis.The data obtained were yield, total phenolics, total tannins, flavonoids and antioxidant activity of black potato peels extracted using ultrasonic bath method with different temperature, extraction time and solvent ratio. The data obtained were analyzed using the Anova test with a significance of α = 0.05. Discussion. The analysis indicated that the treatment of 5 kg/plot manure with an extraction time of 20 minutes, at a temperature of 55°C, and a ratio of ingredients and solvents is 1:10 provided the best quality at 4.13% moisture content, 17.90% yield, 0.177 g/g phenols total, 0.063 g/g tannins, 0.096 g/g flavonoids and 45.275 ppm antioxidant activity.

Aplikasi Pupuk Organik Cair Daun Ketapang dan Penambahan Kapur Dolomit pada Media Tercekam Garam untuk Pertumbuhan dan Hasil Panen Kentang Hitam (Plectranthus rotundifolius) Fatmawati, Mitha Amanda; Andriani, Vivin; Ajiningrum, Purity Sabila
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10909

Black potato production in Indonesia is limited, due to suboptimal cultivation techniques for black potato tubers. Potatoes are generally planted at an altitude above 1000 meters above sea level, because they require low temperatures during the planting process. The aim of this research is to determine the effect of applying liquid organic fertilizer (POC) from ketapang leaves and dolomite lime (CaMgCO3)2 with salt stress media on the growth and yield of black potato plants (Plectranthus rotundifolius). The type of experiment in this research was experimental research using a Randomized Group Design (RAK) which was carried out with 16 treatments, and was repeated 3 times. POC treatment of Ketapang leaves (K) with each concentration, namely K0 (0%), K1 (40%), K2 (60%), K3 (80%), and dolomite lime treatment (C) with each concentration , namely C0 (0g), C1 (20g), C2 (40g), and C3 (80g). Observation variables include plant growth parameters (plant height, number of leaves, stem diameter, and number of branches) and harvest yield parameters (number of tubers and tuber weight). Data analysis used the ANOVA test with a significance level of 0.05. The results of the research showed that giving POC ketapang leaves and dolomite lime had a significant effect on the growth and yield of black potatoes. At plant height, the yield was 24.00 cm, the number of leaves was 25, the number of branches was 5, the stem diameter was 0.90 mm, the number of tubers was 26, and the weight of the tubers was 232 grams.

Aplikasi abu terbang (fly ash) dan abu dasar (bootom ash) terhadap hasil panen dan kadar fitokimia tanaman tomat (Solanum lycopersicum) Andriani, Vivin; Ngadiani, Ngadiani
Teknosains Vol 19 No 1 (2025): Januari-April
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v19i1.51907

Kegiatan pembakaran batu bara di PLTU menyisakan limbah dalam jumlah besar setiap tahunnya. Pembakaran batu bara menghasilkan sekitar 5% polutan padat berupa fly ash (abu terbang) dan bottom ash (abu dasar). Unsur hara lain dalam abu batubara yang dibutuhkan dalam tanah untuk tanaman antara lain B, P, dan unsur-unsur seperti Cu, Zn, Mn, Mo, dan Se. Penelitian ini bertujuan untuk mengetahui pengaruh abu terbang, abu dasar dan kombinasinya terhadap hasil panen dan kandungan fitokimia tanaman tomat (Solanum lycopersicum). Penelitian ini dilakuan di Green House Program Studi Biologi Fakultas Sains dan Teknologi Universitas PGRI Adi Buana Surabaya pada bulan Januari hingga Mei 2024. Penelitian dilakukan secara eksperimental deangan menggunakan RAL (Rancangan Acak Lengkap) dengan 12 faktor dan 3 ulangan yakni k0 (kontrol, tidak diberi apapun), k1 (kontrol positif, pemberian pupuk urea), a1 (400 g/polybag abu terbang), a2 (800 g/polybag abu terbang), a3 (1200 g/polybag abu terbang), b1 (400 g/polybag abu dasar), b2 (800 g/polybag abu dasar), b3 (1200 g/polybag abu dasar), ab1 (200 g/polybag abu terbang + 400g/polybag abu dasar), ab2 (400 g/polybag abu terbang + 200 g/polybag abu dasar), ab3 (600 g/polybag abu terbang + 400 g/polybag abu dasar) dan ab4 (400 g/polybag abu terbang + 600 g/polybag abu dasar). Parameter pengamatan yang diamati meliputi hasil panen (jumlah buah, bobot buah dan diameter buah) serta kadar fitokimia (flavonoid dan antosianin). Data yang didapat dianalisis menggunakan uji Anova dengan signifikansi α = 0,05. Hasil penelitian menunjukkan perlakuan ab4 yang merupakan kombinasi 400 g/polybag abu terbang + 600 g/polybag abu dasar memberikan hasil yang baik pada parameter hasil panen yaitu berat buah dan diameter buah dan serta kadar fitokimia tanaman tomat.

Tepung Labu Kuning (Cucurbita moschata), Tepung Porang (Amorphophallus muelleri Blume), dan Kombinasi Kedua Tepung sebagai Penurun Gula Darah dan Peningkat Motilitas Spermatozoa Mencit (Mus musculus) Sukarjati, Sukarjati; Evarina, Melinda Yuti; Andriani, Vivin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8941

Diabetes Mellitus (DM) is a global epidemic with high morbidity and mortality. Diabetes Mellitus (DM) is thought to have a negative impact on sperm quality. Yellow pumpkin contains antioxidants that can neutralize free radicals which can improve fertility. Glucomannan is a substance contained in porang which functions as a soluble fiber and plays a role in blood glucose balance. This study aims to examine pumpkin flour, porang flour and a combination of both flours on sperm motility and body weight of alloxan-induced diabetic mice. This study used 25 mice aged 3 months, body weight 25gr-30 gr. This study used a completely randomized plan (CRD) with 5 replications and 5 treatments. The treatments were negative control (diabetic mice), positive control (administration of glimepiride at a dose of 0.0078 mg/mice per day), D1 (pumpkin flour 134.4 mg/30g BW of mice), D2 (porang flour 63mg/30g BW mice), D3 (combination 50% : 50% = 67.2 mg : 31.5 mg/30 grBW mice). Diabetic mice were made by intraperitoneal induction of alloxan dose of 200 mg/Kg for 3 days. The parameters observed were spermatozoa motility, body weight and blood sugar levels of the mice. The research data were analyzed using the ANOVA test followed by the BNT test. The results showed that the administration of glymeride, pumpkin flour, porang flour and their combination had a significant effect on reducing blood sugar (p=0.000) and sperm motility (p=0.000), but had no effect on body weight (p=0.317). Conclusion: pumpkin flour, porang flour or a combination of both flours can be used as herbs to lower blood sugar and increase sperm motility in diabetes sufferers.

Pertumbuhan Tanaman Tomat (Solanum lycopersicum) dengan Media Abu Terbang (Fly Ash) dan Abu Dasar (Bottom Ash) Andriani, Vivin; Ajiningrum, Purity Sabila; Ngadiani, Ngadiani
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9272

The use of fly ash and bottom ash as a plant medium can reduce waste from burning coal. The ash waste contains micro and macro nutrients that plants can use for growth. The ash contains the elements Na, K, Ca, Mg, Cu, Zn, and MN. This research aims to determine the effect of fly ash, bottom ash, and their combination on the growth of tomato plants (Solanum lycopersicum). This research used RAL (Completely Randomized Design) with 12 factors and 3 replications, namely k0 (negative control, not given anything), k1 (positive control, given urea fertilizer), a1 (400 g/polybag fly ash), a2 (800 g/polybag fly ash), a3 (1200 g/polybag fly ash), b1 (400 g/polybag base ash), b2 (800 g/polybag base ash), b3 (1200 g/polybag base ash), ab1 (200 g/polybag fly ash + 400 g/polybag fly ash), ab2 (400 g/polybag fly ash + 200 g/polybag fly ash), ab3 (600 g/polybag fly ash + 400 g/polybag fly ash), and ab4 (400 g/polybag fly ash + 600 g/polybag fly ash). The observation variable is the growth of tomato plants, including plant height, number of leaves, number of branches, stem diameter and wet weight. The results showed that there was an effect of using fly ash and bottom ash as a planting medium on the growth of tomato plants for 90 DAP in the AB4 treatment. The combination of 400 g/polybag fly ash + 600 g/polybag fly ash gave good results in plant height, number of leaves, number of branches, and fresh weight of tomato plants, but in terms of branch diameter, NPK treatment gave good results.

Aplikasi Pupuk Organik Cair Daun Ketapang dan Penambahan Kapur Dolomit pada Media Tercekam Garam untuk Pertumbuhan dan Hasil Panen Kentang Hitam (Plectranthus rotundifolius) Fatmawati, Mitha Amanda; Andriani, Vivin; Ajiningrum, Purity Sabila
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10909

Black potato production in Indonesia is limited, due to suboptimal cultivation techniques for black potato tubers. Potatoes are generally planted at an altitude above 1000 meters above sea level, because they require low temperatures during the planting process. The aim of this research is to determine the effect of applying liquid organic fertilizer (POC) from ketapang leaves and dolomite lime (CaMgCO3)2 with salt stress media on the growth and yield of black potato plants (Plectranthus rotundifolius). The type of experiment in this research was experimental research using a Randomized Group Design (RAK) which was carried out with 16 treatments, and was repeated 3 times. POC treatment of Ketapang leaves (K) with each concentration, namely K0 (0%), K1 (40%), K2 (60%), K3 (80%), and dolomite lime treatment (C) with each concentration , namely C0 (0g), C1 (20g), C2 (40g), and C3 (80g). Observation variables include plant growth parameters (plant height, number of leaves, stem diameter, and number of branches) and harvest yield parameters (number of tubers and tuber weight). Data analysis used the ANOVA test with a significance level of 0.05. The results of the research showed that giving POC ketapang leaves and dolomite lime had a significant effect on the growth and yield of black potatoes. At plant height, the yield was 24.00 cm, the number of leaves was 25, the number of branches was 5, the stem diameter was 0.90 mm, the number of tubers was 26, and the weight of the tubers was 232 grams.

Prebiotic And Enzymatic Indices of Split Gill Mushroom (Schizophyllum Commune) Sopandi, Tatang; Putri Hanifah, Riza; Ardithasari, Bunga; Andriani, Vivin
Indonesian Journal of Microbiology Vol. 2 No. 3 (2025): October
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/ijm.v2i3.5095

This study aims to evaluate the prebiotic activity and index as well as the enzymatic activity and index of the split gill mushroom (Schizophyllum commune) through the characterization of the extract and its mycelial part. The extract was prepared through water maceration for 24 hours, then tested for its prebiotic ability against Lactobacillus casei bacteria and its inhibition against Escherichia coli. Inulin was used as a positive control to compare the effectiveness of the extract in supporting the growth of beneficial bacteria and suppressing pathogenic bacteria. The mycelial portion was grown under controlled conditions and tested for amylase, cellulase, and protease activity using fundamental enzymatic techniques commonly used in biochemical research. The results showed that the mushroom extract of split gill was able to significantly increase the growth of L. casei and showed the ability to inhibit the growth of E. coli. As a reflection of supporting the growth of L. casei bacteria and inhibiting E. coli, the prebiotic index of split gill mushroom extract in this study was higher than inulin. Analysis of the split gill mushroom mycelium confirmed the presence of amylase, cellulase, and protease activities with consistent and reproducible values, although the activities were still lower than those of the commercial enzymes used as a comparison in this study. These findings indicate that the bioactive components of split gill mushrooms have relevant capacity for microbial-based applications. The bioactivity profile obtained from this study strengthens the potential of split gill mushrooms as a source of natural prebiotic compounds and as a producer of multifunctional enzymes that can be utilized in various food biotechnology processes. These results open opportunities for the development of innovative formulations that not only support the growth of probiotic bacteria but also provide additional enzymatic activity to improve bioconversion efficiency in various industrial applications.

Antibacterial Effectiveness Test of Ethanol Extract of Gadung Mango Leaves Against Staphylococcus aureus and Escherichia coli Bacteria Indira Pipit Miranti; Vivin Andriani
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.74

Infection is a disease that often occurs in Indonesia. This disease is caused by pathogenic microbes, one of which is bacteria. Staphylococcus aureus and Escherichia coli are bacteria that cause infection. Pharmacological therapy for bacterial infections used to use antibiotics, currently tends to use drugs that come from nature. One of the plants that has the potential as an antibacterial is gadung mango leaves. This study aims to determine the effectiveness of mango leaf ethanol extract against S.aureus and E.coli bacteria. The method used was well diffusion with concentrations of ethanol extract of mango leaves 0.5% (S1), 0.75% (S2), 1% (S3), streptomycin (K+), and ethanol (K-), respectively treatment was repeated 5 times. Data that can be analyzed using One Way ANOVA with a significant level of 0.05. Based on the results of the antibacterial extract of ethanol extract of mango gadung leaves, it has the potential to be better on E. coli bacteria, namely at a concentration of 1% (S3) of 16.05 mm, whereas on S.aureus K+ bacteria (Streptomycin) it is better on control + of 15.9 mm.

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