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Sudarsono Sudarsono
Departemen Agronomi Dan Hortikultura (AGRO-HORT), Fakultas Pertanian, Institut Pertanian Bogor, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Industrial & Manufacturing Engineering

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Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Sholeh Avivi; Sudarsono Sudarsono; Satriyas Ilyas; Hajrial Aswidinnoor
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
Resistensi 10 Galur Kacang Tanah Hasil Silangan antara Arachis cardenassii dan A. hypogaea terhadap Infeksi Peanut stripe virus (PStV) Ahmad Riduan; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12117

Abstract

One of the pathogen infecting peanut in Indonesia is Peanut stripe virus (PStV,), causing stripe and blotch symptoms on infected peanut leaves. The objectives of this research were to evaluate effects of PStV infection on yield of 10 introgression lines of peanut derived from crossing of Arachis cardenasii and A. hypogaea, and to determine the tolerance of these lines against PStV infection. Peanut plants were grown in polybag containing 10 kg of potting soils and were grown under glasshouse conditions. The experimental unit consisted of four plants grown separately in four containers and for each treatment was replicated four times. Peanut plants were inoculated mechanically with Bogor isolate of PStV at 15 days after planting (dap) and harvested at 95-100 dap. Results of the experiment indicated peanut cv. Gajah belonged to moderate tolerance while Kelinci was sensitive against PStV infection. Introgression line NC-CS11, CS30 and WS4 were grouped as tolerance while NC-CS51, WSl, and WS3 were moderate tolerance. The tolerance lines showed mild mosaic symptoms, did not show reduction of plant height and peanut yield upon inoculation with PStV. Introgresion line NC-CS15, CS20,CS22, and CS50 were sensitive against PStV infection, showed moderate to severe mosaic/blotch symptoms, reduction of plant height and peanut yield due to PStV infection. Among the tolerance and moderate tolerance lines, only NC-CS30 showed higher yield as compared to peanut cv. Gajah or Kelinci. Therefore, this line may be developed further as commercial peanut cultivar or be use as donor germplasm for PStV tolerance mechanisms in peanut breeding.
Deteksi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Komersial yang Beredar di Indonesia Aswaldi Anwar; Satriyas Ilyas; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12118

Abstract

The existence of Clavibacter michiganensis subsp. michiganensis (Cmm), the pathogen causing bacterial canker, on 22 lots of tomato seeds distributed commercially in Indonesia was evaluated. Isolation of suspected Cmm colonies were conducted by agar dilution plating on semiselective SCM medium followed by confirmation of colony identity on YDC medium. Identity of suspected Cmm colony was confirmed using IF test, pathogenicity test on tomato seedlings, hypersensitivity test on leaf of Mirabilis jalapa and Nicotiana tabacum, ELISA, and Cmm specific DNA amplification by PCR. After seed extraction and evaluation of the extract on semi-selective SCM medium and confirmastion by IF test, at least six tomato seed lots were contaminated with Cmm. After more confirmation using pathogenicity and hypersensitivity test, ELISA, and PCR amplification, at least three seed lots were confirmed possitively to carry Cmm.
Variabilitas Strain-Strain PStV pada Tingkat Molekul Berdasarkan Gen Protein Selubung dan 3' UTR (Untranslated Region) Genom RNA PStV Hasriadi Mat Akin; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12318

Abstract

Variability of various peanut stripe virus (PStV) strains at the molecular level was studied based on nucleotide sequence of coat protein (CP) cistron and 3' UTR (untranslated region). Six PStV strains causing different types of symptoms on peanut leaves (Arachis hypogaea var. Landak) were used in this study. Fourteen point mutations on the coat protein gene have been identified among the PStV strains, however, only two point mutations at three positions (9309 of PStV-lsb and PStV-lbs and 9885 of PStV-Ibs) resulted in amino acid residue changed of the coat protein. Pair comparison among six 3' UTR sequences of the strains revealed a 97.9 - 100% sequence similarity. The results provided evidence that all the strains were of the same virus and there is no correlation between typical symptoms of the PStV infection and CP sequence variability among the strains.
Pengaruh Komposisi Media terhadap Pembentukan Kalus Embriogenesis Somatik Kopi Arabika ( Coffea arabica ) Meynarti Sari dewi Ibrahim; Sudarsono Sudarsono; Rubiyo Rubiyo; Syafaruddin Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 3, No 1 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n1.2012.p13-22

Abstract

Induksi embrio somatik pada kopi arabika (Coffea arabica) dengan menggunakan beberapa zat pengatur tumbuh (ZPT) telah berhasil dilakukan. Pengaruh komposisi media terutama kombinasi antara jenis ZPT yang berbeda dan tanggap genotipe tanaman dilaporkan sangat bervariasi. Tujuan penelitian untuk mengkaji pengaruh pemberian 2,4-D dan kinetin dalam proses pembentukan dan pertumbuhan kalus embriogenik asal daun. Penelitian dilakukan di Laboratorium Pusat Penelitian dan Pengembangan Perkebunan (Puslitbangbun) Agustus 2011 sampai Januari 2012. Bahan tanaman yang digunakan adalah daun dari kopi arabika varietas Sigarar Utang yang merupakan tanaman koleksi Balai Penelitian Tanaman Industri dan Penyegar (Balittri). Penelitian menggunakan rancangan acak lengkap dengan 5 ulangan, masing-masing ulangan terdiri dari 5 eksplan. Peubah yang diamati, meliputi persentasi kalus yang terbentuk, morfologi kalus, berat basah kalus, dan jumlah globular. Hasil menunjukkan semua perlakuan dapat membentuk kalus, pertambahan berat eksplan tertinggi diperoleh pada media kombinasi 2,4-D 1 mg/l atau 2 mg/l dan kinetin 1 sampai 4 mg/l. Embrio somatik terbanyak diperoleh pada media yang diberi 2,4-D 0,5 mg/l dan kinetin 1 mg/l. Selain kalus, massa proembrio dan embrio, juga terbentuk akar adventif yang jumlahnya tidak nyata antar perlakuan.  The Effect of Composition Media to Callus Formation of Somaticembryogenesis of Arabica Coffee (Coffea arabica)ABSTRACT Induction of somatic embryos with plant growth regulators (PGR) has successfully performed in arabica coffee. However, the influence of media composition combined with different PGR, explants and genotype of plants is widely various in response yields. The objective of this study was to examine the effects of 2,4-D and kinetine in process of formation and growth of embryogenic callus developed from leaves of arabica coffee. The studiy was carried out at a laboratory of Indonesian Research Center for Estate Crops (Puslitbangbun) from August 2011 to January 2012. Plant materials used are coffee leaves var. Sigarar Utang taken from a germplasm collection of the crop grown at Pakuwon Research Station, Indonesian Research Institute for Industry Crops (Balittri) located at Sukabumi, West Java. A completely randomized design with 5 replications and plot size of five explants was used. Parameters observed are percentage of callus formation, morphology of the callus, fresh weight of callus, and number of globular. Results show that all treatments examined are able to form callus. The highest increase in weight of explants was obtained from the media treated with 2,4-D (conc. of 1mg/l or 2 mg/l) and kinetin (conc. of 1 to 4 mg/l). While, the most number of somatic embryo formed was obtained from those of treated with 2,4-D 0.5 mg/l and kinetin 1 mg/l. In addition to callus formation, proembryo mass, embryo and adventive roots were also formed in spite of not significant between different the treatments.
KERAGAMAN GENETIKA VARIAN ABAKA YANG DIINDUKSI DENGAN ETHYLMETHANE SULPHONATE (EMS) RULLY DYAH PURWATI; SUDJINDRO SUDJINDRO; ENDANG KARTINI; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 14, No 1 (2008): Maret 2008
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v14n1.2008.16-24

Abstract

ABSTRAKMutasi in vitro dengan perlakuan mutagen dapat digunakan untukmeningkatkan keragaman genetika abaka melalui keragaman somaklonal.Penelitian yang dilakukan bertujuan untuk: (1) menentukan konsentrasioptimum EMS untuk induksi keragaman somaklonal dalam kultur kalusembriogen abaka, (2) meregenerasikan bibit abaka varian dari kalusembriogen yang diberi perlakuan EMS, dan (3) mengevaluasi tipe danfrekuensi keragaman karakter kualitatif dan kuantitatif di antara populasitanaman mutan abaka yang diperoleh dari regenerasi kalus embriogenyang diberi perlakuan EMS. Penelitian dilakukan mulai bulan Agustus2003 sampai Agustus 2006 di Laboratorium Kultur Jaringan dan KebunPercobaan Karangploso, Malang pada Balai Penelitian TanamanTembakau dan Serat, Malang (Balittas). Hasil penelitian menunjukkanbahwa 0,6% EMS merupakan konsentrasi optimum karena padakonsentrasi tersebut diperoleh keragaman somaklonal paling banyak.Varian yang diperoleh menunjukkan karakter kualitatif dan kuantitatifabnormal. Tipe varian tersebut umumnya bersifat negatif dan kurangmenguntungkan dibandingkan dengan populasi standar. Tipe dan frekuensikeragaman kualitatif dan kuantitatif pada klon Tangongon berbeda denganklon Sangihe-1, mengindikasikan adanya pengaruh genotipe terhadapkeragaman somaklonal. Varian dari abaka klon Tangongon denganproduksi serat tertinggi (161,0 g dan 154,0 g/tanaman) diperoleh dariperlakuan EMS 0,3% (T1 28.1.1 dan T1 11.2.2), sedangkan dari klonSangihe-1, hasil serat tertinggi (35,0 g dan 40,0 g/tanaman) diperoleh dariperlakuan EMS 0,6% (S4 28.1.0 dan S4 56.2.0). Produktivitas tersebutlebih rendah dibandingkan dengan produksi serat tanaman kontrol klonTangongon (193,0 g/tanaman) dan Sangihe-1 (70 g/tanaman).Kata kunci : Abaka, Musa textilis, keragaman somaklonal, EMS, muta-genesis, in vitro, hasil, Jawa TimurABSTRACTGenetic Variability of Abaca Variants Induced byEthylmethane Sulphonate (EMS)In vitro mutation with mutagen treatment can be used to increasethe genetic variability of abaca by inducing somaclonal variation. Theobjectives of the experiments were to (1) determine optimum concen-tration of EMS to induce abaca somaclonal variation, (2) produce abacalines from EMS treated embryogenic calli and evaluate their performancein the field, and (3) evaluate type and frequency of qualitative andquantitative variant characters among regenerated abaca lines. Thisexperiment was conducted in Tissue Culture Laboratory and KarangplosoExperiment Station of Indonesian Tobacco and Fibre Crops ResearchInstitute (ItoFCRI) Malang from August 2003 to August 2006. The resultsshowed that EMS treatment on abaca embryogenic calli induced variation,and the optimum EMS concentration was 0.6%. The variants exhibited anumber of abnormal qualitative and quantitative characters which weregenerally negative characters since they showed lower value as comparedto control population. The presence of different types of qualitative andquantitative variant characters was genotype dependent. Variants fromabaca clone Tangongon having the highest fibre yield (161.0 g and 154.0g/plant) were obtained from 0.3% EMS treatment (T1 28.1.1 and T1 11.2.2variants). While variants from abaca clone Sangihe-1 having the highestfibre yield (35.0 g and 40.0 g/plant) were obtained from 0.6% EMStreatment (S4 28.1.0 and S4 56.2.0 variants). The fibre yield of controlclones, Tangongon and Sangihe-1, were 193.0 g and 70 g/plant,respectively.Key words: Abaca, Musa textilis, induced mutation, somaclonalvariation, field evaluation, yield, East Java
PENGEMBANGAN PENANDA MOLEKULER BERDASARKAN SITUS SNP DAN INDEL GENOM KLOROPLAS KELAPA Freta Kirana Balladona; Ismail Maskromo; Dewi Sukma; Sudarsono Sudarsono
JURNAL AGRONIDA Vol. 6 No. 1 (2020)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.196 KB) | DOI: 10.30997/jag.v6i1.2548

Abstract

Saat ini informasi dasar mengenai silsilah, keragaman dan hubungan evolusi kekerabatan menggunakan marka molekuler pada kelapa di Indonesia masih kurang. Hal ini dibuktikan dengan belum banyak dilaporkan urutan sekuens genom kelapa Indonesia yang dapat dijadikan dasar dalam pembuatan marka molekuler tersebut. Salah satu genom tanaman yang dapat dimanfaatkan sebagai penanda adalah sekuens genom kloroplas (cpDNA). Genom kloroplas merupakan penanda yang efisien untuk mempelajari evolusi dan sejarah populasi tanaman melalui filogenetik karena bersifat sangat konservatif, diwariskan secara maternal, memiliki ukuran yang lebih kecil dibandingkan dengan genom inti. Tujuan dari penelitian ini adalah pengembangan primer berdasarkan genom kloroplas berbasis situ SNP dan indels. Berdasarkan sembilan sekuens genom kloroplas pada tanaman palma, telah berhasil didisain 10 primer berdasarkan situs SNP dan 5 primer berdasarkan situs insersi delesi. Hasil validasi primer tersebut menggunakan DNA kelapa Indonesia didapatkan hasil bahwa 10 primer SNP berhasil teramplifikasi sedangkan indels hanya 2 primer berbasis PCR.
Co-Authors AHMAD RIDUAN Aniversari Apriana Anwar, Aswaldi Atmitri Sisharmini Diah Manohara Diani Damayanti Djoko Santoso Djoko Santoso ENDANG KARTINI Endrizal ; Freta Kirana Balladona H. Aswidinnoor HAJRIAL ASWIDINNOOR Hasriadi Mat Akin Ika Mariska Ilham Nur Ardhi Wicaksono Ismail Maskromo Kurniawan Rudi Trijatmiko M. Herman Meynarti Sari Dewi Ibrahim Muhammad Herman Nurul Khumaida Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo RULLY DYAH PURWATI Satriyas Ilyas Sholeh Avivi Sri H Hidayat Sri H. Hidayat Sri Hendrastuti SUDJINDRO SUDJINDRO Sukma, Dewi Syafaruddin Syafaruddin Syamsudin Syamsudin Tri Joko Santoso Tri Joko Santoso Wening Enggarini Yusniwati Yusniwati