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Association of Liver X Receptor Alpha (LXRα) gene related to characteristic of carcass, meat quality and fatty acid composition in ducks Gunawan, A.; Azmi, N.; Anggraeni, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.114-123

Abstract

Liver X Receptor Alpha (LXRα) is a nuclear receptor that play a crucial role in regulating of gene involvedin lipid metabolism. The aim of this research was to identify polymorphisms and association of LXRα gene with charateristic of carcass, meat quality and fatty acid composition in ducks using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). A total sample of 98 Cihateup ducks consisted of 57 females and 41 males with age 12 weeks were used in this study. Product size is 661 bp amplicons. The genotype genes frequencies in CC, GC and GG were 0.21, 0.55 and 0.23 respectively. The chi-square test revealed that LXRα gene (g.3575 C>G) in exon 2 was in Hardy-Weinberg equilibrium. A SNP of LXRα gene in region g.3575 C>G was significantly associated (P<0.05) with duck meat quality and fatty acid content. Several parameters have significant affect (P <0. 05) on meat quality in the breast meat weight, carcass percentage and head percentage, while associated fatty acids were saturated fatty acids (SFA) such as palimitic acid (C16:0); γ-and unsaturated fatty acids (UFA) such as linolenic acid (C18:3n3); cis 11 eicosenoic acid (C20:1) and 11.14 cis-eicosedenoic acid (C20:2). In could be concluded that LXRα gene might be useful as genetic markers to select and produce meat with desirable unsaturated fatty acids.
Heritability estimation and non-genetic factors affecting production traits of Indonesian Ongole cross . Hartati; . Muladno; . Jakaria; R. Priyanto; A. Gunawan; . Aryogi; Chalid Talib
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.514 KB) | DOI: 10.14334/jitv.v20i3.1183

Abstract

Productivity data from 560 head of PO cattle have been collected for 9 years from 2004 until 2013 for evaluating heritability estimation and environmental factors affecting production traits of Indonesian Ongole cross from birth to yearling old. Heritability estimation were analyzed using varians analysis with data of paternal halfsib correlation. Varians component for to estimate of heritability value were analyzed using completely randomized design one - way classification. While a general linear model was used to analyze the environmental factors (sex, year, parity, season and type of births). The results showed that sex of calves and year of births had significant differences (P<0.05) for all production traits such as weight at birth, weaning and yearling, while the type of birth only presented a significant difference on birth weight. Parity, season and generation exhibited nonsignificantly effect on those production traits. Estimation of heritability on birth weight, weaning weight and yearling weight were 0.28+0.12; 0.47+0.15 and 0.63+0.17 respectively. The highest heritability values obtained in yearling weight, this means that yearling weight will be effective as selection criteria to improve the genetic of PO cattle.
APLIKASI MOBILE VOTING BERBASIS ANDROID UNTUK PEMILIHAN KETUA OSIS DENGAN MODEL RAD STUDI KASUS SMAN 1 PARUNGKUDA A. Gunawan; Ramdhan Saepul Rohman; Andika Purnama; Gunawan Gunawan
Swabumi Vol 6, No 2 (2018): Volume 6 Nomor 2 Tahun 2018
Publisher : Universitas Bina Sarana Informatika Kota Sukabumi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31294/swabumi.v6i2.4567

Abstract

Abstrak Penggunaan voting sebagai media dalam membuat keputusan yang berkaitan dengan hajat hidup orang banyak telah dimulai sejak lama. Voting merupakan kegiatan yang sangat menentukan pada setiap perhelatan pemilihan, banyak varian kepentingan yang harus diakomodir di dalamnya, bagaimana regulasi atau peraturan yang disepakati dan menjadi aturan main, siapa yang dipilih dan siapa yang berhak memilih. SMA Negeri 1 Parungkuda selama ini dalam menentukan pemilihan ketua osis menggunakan metode voting (pemungutan suara) secara konvensional yaitu dengan cara semua siswa pada setiap kelasnya diberikan lembar yang berisi nama calon-calon yang dimajukan sebagai calon ketua osis, kemudian siswa akan menceklis nomor salah satu dari calon tersebut. RAD merupakan perangkat lunak yang menekankan siklus perkembangan dalam waktu yang singkat. RAD menggunakan metode berulang dalam mengembangkan sistem dimana model bekerja sistem dikonstruksikan di awal tahap pengembangan dengan tujuan menetapkan kebutuhan pengguna. Dalam penulisan skripsi ini di terapkan teknologi mobile voting berbasis android dengan model RAD untuk pemilian ketua osis pada SMA Negeri 1 Parungkuda. Kata Kunci: Voting, Mobile Voting, RAD, SMAN 1 Parung kuda
Regional Analysis of Sheep Business in Banjarnegara Regency E. Gustia; S. Mulatsih; A. Gunawan
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 2 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.2.105-111

Abstract

Banjarnegara Regency is home to the local Batur sheep, known for their significant body weight. The community has been raising Batur sheep for generations, aiming to develop them into a regional economic driver. This study aims to analyze potential areas for sheep livestock development in Banjarnegara Regency. The data used came from the farm statistics of the Directorate-General for Farm and Animal Health in 2021. The location quotient (LQ), localization index (LI), and specialization index (SI) method were used to perform regional analysis of sheep business. The results of the survey showed that the base area of sheep farming in Banjarnegara district is distributed in the districts of Karangkobar, Pejawaran, Batur, and Wanayasa. The localization and specialization index showed that sheep farming is spread evenly throughout the district of Banjaregara, not concentrated in a particular region.
PENDUGAAN BOBOT BADAN MELALUI ANALISIS MORFOMETRIK DENGAN PENDEKATAN REGRESI TERBAIK BEST - SUBSET PADA DOMBA GARUT TIPE PEDAGING, TANGKAS DAN PERSILANGANNYA GUNAWAN, A.; JAMAL, K.; SUMANTRI, C.
Majalah Ilmiah Peternakan Vol 11 No 1 (2008)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.281 KB)

Abstract

ABSTRACT ESTIMATE OF BODY WEIGHT FIGHTING AND MEAT GARUT SHEEP AND CROSSBREED WITH MERFOMETRIC ANALYSIS APPROACH The aims of this study were to estimate body weight and to determine the best equation which can be used to estimate body weight of fighting and meat Garut sheep and its crossbreed type at district and outdistrict Garut. This study used primary data of Garut sheep from Garut district consisting of Margawati, Wanaraja and Sukawening. For outdistrict Garut, it was used Garut sheep from Bogor consisting of Ciomas and Cinagara. The total number of sheep collected were 531 heads. The differences of body measurements locations were analyzed by t-test, Correlation, and Regression Multiple Linear Regression and Polynomial Regression (Linear, Quadratic, and Cubic) Analyses. The results showed that body weight of fighting and meat rams and ewes from Wanaraja were the highset than another Garut sheep and crossbreed. The highest body measurements were found from Wanaraja (fighting and meat type) and Sukawening (fighting type) sheep and the lowest body measurements were found from Margawati and Cinagara. Whilst that some body measurements of rams were not different (P>0.05). Correlation coefficient between body weight and various body measurements body lenght, weither height, chest deep and chest circumference of Garut Sheep were highest at district and outdistrict of Garut. The best regression equations to describe the relation ship between body weight and body measurements for rams and ewes Garut sheep at district and outdistrict Garut were multiple regression equations. The highest determination coefficient (100%) was shown for meat type from Wanaraja and the lowest one (93.93%) was shown of fighting type from Sukawening. ABSTRAK Penelitian ini bertujuan untuk menduga bobot badan dan mencari model terbaik dalam menduga bobot badan domba Garut tangkas, pedaging dan persilangannya di daerah Garut dan luar Garut. Total domba yang digunakan pada penelitian ini sebanyak 531 ekor domba Garut dari beberapa lokasi di kabupaten Garut (Margawati, Wanaraja, Sukawening) dan di Luar Garut (Ciomas dan Cinagara). Data dari hasil penelitian dianalisis dengan menggunakan Uji-t, Korelasi, dan Regresi meliputi Analisis Regresi Linier Ganda dan Analisis Regresi Polinomial (Linier, Kuadratik, dan Kubik). Hasil Uji-t menunjukkan, bahwa rataan bobot badan domba tangkas Garut baik jantan maupun betina dari Wanaraja memiliki bobot badan paling besar dibandingkan dengan domba Garut lain dan persilangannya. Ukuran tubuh tertinggi pada domba Garut ditunjukkan pada domba tangkas dan pedaging Wanaraja serta tangkas Sukawening dan ukuran tubuh terkecil ditunjukkan pada domba Garut di Margawati dan Cinagara. Koefisen Korelasi tertinggi antara bobot badan dan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging da persilanganya ditunjukkan antara bobot badan dengan panjang badan, tinggi pundak, dalam dada dan lingkar dada. Persamaan regresi yang paling baik digunakan menduga bobot badan berdasarkan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging dan persilangannya adalah Persamaan Regresi Linier Ganda dengan tingkat akurasi berkisar antara 93,3%-100%. Tingkat akuras tertinggi ditunjukkan pada domba tangkas Wanaraja dengan tingkat akurasi 100% dan terendah ditunjukkan pada domba tangkas di Sukawening dengan tingkat akurasi 93,93%.
Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross Furqon, A.; Gunawan, A.; Ulupi, N.; Suryati, T.; Sumantri, C.
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.827 KB) | DOI: 10.5398/medpet.2017.40.3.151

Abstract

Stearoyl-CoA desaturase (SCD) is an integral membrane protein of endoplasmic reticulum (ER) that catalyzes the rate limiting step in the monounsaturated fatty acids from saturated fatty acids. Selection for fatty acids traits based on molecular marker assisted selection is needed to increase a value of chicken meat. This study was designed to analyze expression and associations of SCD gene polymorphisms with fatty acid traits in F2 kampung-broiler chicken cross. A total of 62 F2 kampung-broiler chicken cross (29 males and 33 females) were used in this study. Fatty acid traits were measured at 26 weeks of age. Samples were divided into two groups based on fatty acid traits (the highest and the lowest). Primers in exon 2 region were designed from the genomic chicken sequence. The SNP g.37284A>G was detected and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was then used to genotype. The expression of SCD gene was analyzed using quantitative real time PCR (qRT-PCR). The result showed that there were three genotypes (AA, AG, and GG) found in this study. The SCD|AciI polymorphism was significantly associated with palmitoleic acid (C16:1), fatty acids total and saturated fatty acid in 26 weeks old of F2 kampung-broiler chicken cross (P<0.05). The SCD gene was expressed for polyunsaturated fatty acids in liver tissue in two groups of chickens. In conclusion, the SCD gene could be a candidate gene that affects fatty acids traits in F2 kampung-broiler chicken cross.
Association of Genetic Polymorphism TP53INP1 Gene with Mineral Content in Javanese Thin-Tailed Sheep Listyarini, K.; Harahap, R. S.; Roosita, K.; Sumantri, C.; Mulyono, R. H.; Gunawan, A.
Jurnal Sain Peternakan Indonesia Vol 20 No 2 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.2.88-92

Abstract

The Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1) gene is expected to be a crucial candidate for mineral content.  This research aimed to analyse the genotype polymorphism and investigate its association with the TP53INP1 gene and sheep mineral content. To analyse gene polymorphisms and conduct an association study, 30 rams of Javanese thin-tailed sheep were utilised. The NlaIII restriction enzyme was applied to investigate the genotype polymorphism of the TP53INP1 gene through Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Regarding the association with analysing TP53INP1, a General Linear Model (GLM) was used. The findings indicate that the TP53INP1 gene shows polymorphism, revealing three distinct genotypes identified by the results: AA, AG, and GG. The genotypes were detected using the Hardy-Weinberg Equilibrium (HWE) principle. According to the association analysis, a significant association (P < 0.05) was found between the TP53INP1 gene and mineral content, particularly iron (Fe). A higher mineral content was associated with the GG genotype, whereas a lower mineral content was related to the AA genotype. The TP53INP1 could be the candidate gene for sheep's mineral content.
Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins Anwar, P.; Maulana, T.; Sumantri, C.; Arifiantini, I.; Gunawan, A.
Tropical Animal Science Journal Vol. 48 No. 5 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.5.393

Abstract

Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.
Identifikasi Keragaman Gen Flavin-Containing Monooxygenases 3 (FMO3|AlwNI) pada Ayam Lokal Indonesia Suhita, R. L.; Gunawan, A.; Darwati, S.; Ulupi, N.; Sumantri, C.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 3 No. 3 (2015): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Local chicken in Indonesia is commonly maintained traditionally for meat consumption so that ithas the potential to be developed as an economical livestock commodities. The increasing of meat local chicken demand should be followed by the increasing of its quality. FMO3 gene as a gene that control meat quality especially controlling fish odor in livestock product, can be used as a functional gene in its function as a genetic marker. This research was aimed to identify the FMO3 gene polymorphism in exon 6 inbroiler, kampung,sentul, merawang, pelung and nunukan chicken population. Identification of polymorphism was used 129 samples from those populations. AlwnIenzyme was used in PCR-RFLP technique to digest PCR product overnight.Genotypingshowed only AA genotype (275/249 bp) in 524 bp product amplification. There was no polymorphism found in every population. It needs further study in the different sequences to findthe polymorphism of FMO3 in local chicken.
Sifat Fisik dan Aktivitas Antioksidan Dendeng Daging Sapi dengan Penambahan Stroberi (Fragaria ananassa) sebagai Bahan Curing Kosim, A.; Suryati, T.; Gunawan, A.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 3 No. 3 (2015): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nitrate or nitrite that used on dendeng making as antioxidant agent and color stabilizer could forming toxic compound (nitrosamine). The Formation of nitrosamine could be inhibited by addition of natural antioxidant that contained in the fruits such as strawberry (Fragaria ananassa). The aim of this study was to evaluate the influence of stawberry as curing ingredient on physical properties and antioxidant activity of dendeng. Dendeng was made by addition of spices and combination of nitrite (0 ppm and 125 ppm) and strawberry (0%, 10%, 20%, 30%) addition. Sample for analysis used fried dendeng. The result showed that pH value  were  affected  by  additon  of  nitrite  and  strawberry    (P<0.01),  but  the objective color was affected by addition of nitrite only. Strawberry addition affected antioxidant activity of dendeng that was evaluated by scavenging activity on radical DPPH and antioxidant capacity (P<0.05). Interaction factor affected water activity and total phenolic content of dendeng (P<0.05). As conclusion strawberry addition up to 30% could be used as curing agent on dendeng making and increased antioxidant activity on dendeng product.