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Journal : Jaringan Laboratorium Medis

Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor Dina Afrianti; Elis Susilawati; Eko Naning Sofyanita
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8445

Abstract

Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.
Kejadian Toksoplasmosis pada Wanita Hamil Pemelihara Kucing dan Wanita Hamil Bukan Pemelihara Kucing di Puskesmas Tlogosari Wetan Kota Semarang Dina Afrianti; Adita Puspitasari Swastya Putri; Wahyudi Wahyudi
Jaringan Laboratorium Medis Vol 5, No 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.9396

Abstract

Toxoplasma gondii (T. gondii) is a widespread zoonotic pathogen worldwide. This parasite grows inside the body of a cat as definitive host. Cats play an important role in the epidemiology of T. gondii because they are the only animal capable to shed oocysts which can withstand outside environment. Nowadays, transmission of T. gondii infection from cat to human increases in particular areas. This study aims to identify the incidence of toxoplasmosis in pregnant women Raise Cats and Not Raise Cats in Tlogosari Wetan Health Center Semarang City. This study was an descriptive observational research with case control design in two populations. Subjects are chosen based on certain criteria, This study was done to 25 respondents as pregnant women raise cats and 25 respondents who weren’t. The results obtained from serological analysis on 50 blood serum samples consisting of 25 blood serum samples of pregnant women raise cats with positive IgG antibody as many as 6 serum samples (24%) and 25 blood serum samples of pregnant women who are not raise cats obtained positive IgG antibody in 2 samples serum (8%). The total percentage of toxoplasmosis was 16% from 50 serum samples. It is necessary to carry out a large-scale serological survey to determine the prevalence of toxoplasmosis in pregnant women in Semarang City and necessary monitoring and treatment of pregnant women infected with Toxoplasma gondii.
The Relationship of Zink and Iron Levels with Blood Pressure in Pulmonary Tuberculosis Patient Dewi Kurniasih; Muslina Muslina; Aminahtun Latifah; Dina Afrianti
Jaringan Laboratorium Medis Vol 5, No 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10415

Abstract

Background: Mycobacterium tuberculosis infection in Tuberculosis (TBC) causes narrowing of the pulmonary blood vessels and triggers pulmonary hypertension which is associated with iron deficiency and anemia. The mineral zinc (Zn) is known to play an important role in destroying the bacteria that cause TBC. In addition, iron (Fe) deficiency in TBC patients can cause relapse and even treatment failure. This study aims to examine the relationship between serum zinc and iron levels and blood pressure in pulmonary TBC patients. Method: A descriptive analytical observational study with a cross sectional design was conducted on 51 TBC patients undergoing treatment at the Simpang IV Sipin Community Health Center, Jambi City. Using a purposive sampling technique, respondents who met the research inclusion and exclusion criteria were examined for zinc and Fe levels. The examination was carried out at the Jambi Province Regional Health Laboratory, the examination result data was then analyzed bivariately using the chi square test. Results: The results of statistical analysis between blood pressure and Zn levels obtained a significant value with P value = 0.877 and a significant value for Fe levels obtained P value = 0.841 ( 0.05). Conclusion: There is no significant relationship between Zn and Fe levels and blood pressure in pulmonary TBC patients. Based on research, it is known that iron deficiency and anemia are not directly related to pulmonary hypertension which can be experienced by TBC patients.
Uji Kualitas Telur Cacing STH Menggunakan Pewarna Alami Perasan Daun Jati (Tectona grandis) dengan Konsentrasi 40%, 60%, 80%, 100% sebagai Pewarna Alternatif Metode Sedimentasi Anggraini, Maysita Ari; Afrianti, Dina
Jaringan Laboratorium Medis Vol 6, No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11625

Abstract

Helminthiasis is a parasitic infection in the form of worm larvae or eggs that develop in the human body. Diagnosing helminthiasis with fecal examination uses 2% eosin dye to clarify and emphasize the shape of worm eggs. Eosin 2% is a class-3 IARC carcinogenic reagent, so a natural reagent that does not damage the environment is needed. Reagents from natural materials that can be utilized are teak leaves containing anthocyanin compounds that can clarify and emphasize the shape of worm eggs.  The purpose of this study was to determine the quality of sth (soil transmitted helminth) worm eggs using natural dye teak leaf juice (tectona grandis) with a concentration of 40%, 60%, 80%, 100% as an alternative dye sedimentation method. The design of this study is quantitative with experimental design with posttest with control group design with Kruskal-Walis test. The results of this study obtained the concentration with the highest average value, namely 40% concentration of 8.7. The higher the concentration, the less clear the morphology of the egg because of the influence of dirt / residue from the juice. The results of the Kruskal-Walis test sig value 0.054, it can be concluded that there is no difference between teak leaf juice with a concentration of 40%, 60%, 80%, and 100% with 2% eosin dye as a control. Based on the mean rank value of the Kruskal-Walis test, it can be seen that the best teak leaf juice concentration is 40% concentration with a value of 34.50. This research can be used as a reference for the utilization of teak leaves as a natural dye to replace 2% eosin for STH worm eggs.
Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor Afrianti, Dina; Susilawati, Elis; Sofyanita, Eko Naning
Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8445

Abstract

Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.
Kejadian Toksoplasmosis pada Wanita Hamil Pemelihara Kucing dan Wanita Hamil Bukan Pemelihara Kucing di Puskesmas Tlogosari Wetan Kota Semarang Afrianti, Dina; Swastya Putri, Adita Puspitasari; Wahyudi, Wahyudi
Jaringan Laboratorium Medis Vol. 5 No. 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.9396

Abstract

Toxoplasma gondii (T. gondii) is a widespread zoonotic pathogen worldwide. This parasite grows inside the body of a cat as definitive host. Cats play an important role in the epidemiology of T. gondii because they are the only animal capable to shed oocysts which can withstand outside environment. Nowadays, transmission of T. gondii infection from cat to human increases in particular areas. This study aims to identify the incidence of toxoplasmosis in pregnant women Raise Cats and Not Raise Cats in Tlogosari Wetan Health Center Semarang City. This study was an descriptive observational research with case control design in two populations. Subjects are chosen based on certain criteria, This study was done to 25 respondents as pregnant women raise cats and 25 respondents who weren’t. The results obtained from serological analysis on 50 blood serum samples consisting of 25 blood serum samples of pregnant women raise cats with positive IgG antibody as many as 6 serum samples (24%) and 25 blood serum samples of pregnant women who are not raise cats obtained positive IgG antibody in 2 samples serum (8%). The total percentage of toxoplasmosis was 16% from 50 serum samples. It is necessary to carry out a large-scale serological survey to determine the prevalence of toxoplasmosis in pregnant women in Semarang City and necessary monitoring and treatment of pregnant women infected with Toxoplasma gondii.
The Relationship of Zink and Iron Levels with Blood Pressure in Pulmonary Tuberculosis Patient Kurniasih, Dewi; Muslina, Muslina; Latifah, Aminahtun; Afrianti, Dina
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.10415

Abstract

Background: Mycobacterium tuberculosis infection in Tuberculosis (TBC) causes narrowing of the pulmonary blood vessels and triggers pulmonary hypertension which is associated with iron deficiency and anemia. The mineral zinc (Zn) is known to play an important role in destroying the bacteria that cause TBC. In addition, iron (Fe) deficiency in TBC patients can cause relapse and even treatment failure. This study aims to examine the relationship between serum zinc and iron levels and blood pressure in pulmonary TBC patients. Method: A descriptive analytical observational study with a cross sectional design was conducted on 51 TBC patients undergoing treatment at the Simpang IV Sipin Community Health Center, Jambi City. Using a purposive sampling technique, respondents who met the research inclusion and exclusion criteria were examined for zinc and Fe levels. The examination was carried out at the Jambi Province Regional Health Laboratory, the examination result data was then analyzed bivariately using the chi square test. Results: The results of statistical analysis between blood pressure and Zn levels obtained a significant value with P value = 0.877 and a significant value for Fe levels obtained P value = 0.841 (> 0.05). Conclusion: There is no significant relationship between Zn and Fe levels and blood pressure in pulmonary TBC patients. Based on research, it is known that iron deficiency and anemia are not directly related to pulmonary hypertension which can be experienced by TBC patients.
Uji Kualitas Telur Cacing STH Menggunakan Pewarna Alami Perasan Daun Jati (Tectona grandis) dengan Konsentrasi 40%, 60%, 80%, 100% sebagai Pewarna Alternatif Metode Sedimentasi Anggraini, Maysita Ari; Afrianti, Dina
Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11625

Abstract

Helminthiasis is a parasitic infection in the form of worm larvae or eggs that develop in the human body. Diagnosing helminthiasis with fecal examination uses 2% eosin dye to clarify and emphasize the shape of worm eggs. Eosin 2% is a class-3 IARC carcinogenic reagent, so a natural reagent that does not damage the environment is needed. Reagents from natural materials that can be utilized are teak leaves containing anthocyanin compounds that can clarify and emphasize the shape of worm eggs.  The purpose of this study was to determine the quality of sth (soil transmitted helminth) worm eggs using natural dye teak leaf juice (tectona grandis) with a concentration of 40%, 60%, 80%, 100% as an alternative dye sedimentation method. The design of this study is quantitative with experimental design with posttest with control group design with Kruskal-Walis test. The results of this study obtained the concentration with the highest average value, namely 40% concentration of 8.7. The higher the concentration, the less clear the morphology of the egg because of the influence of dirt / residue from the juice. The results of the Kruskal-Walis test sig value 0.054, it can be concluded that there is no difference between teak leaf juice with a concentration of 40%, 60%, 80%, and 100% with 2% eosin dye as a control. Based on the mean rank value of the Kruskal-Walis test, it can be seen that the best teak leaf juice concentration is 40% concentration with a value of 34.50. This research can be used as a reference for the utilization of teak leaves as a natural dye to replace 2% eosin for STH worm eggs.
Efektivitas Masa Penyimpanan Filtrat Daun Jati (Tectona grandis) terhadap Kualitas Pewarna Preparat Telur STH (Soil Transmitted Helminth) Rahmadhani, Afiffah Gita; Afrianti, Dina; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13018

Abstract

Soil-Transmitted Helminth (STH) infections such as Ascaris lumbricoides remain a public health concern in Indonesia, necessitating microscopic fecal examinations using high-quality stains. Eosin is commonly used but poses environmental and health risks. Anthocyanins found in teak leaf (Tectona grandis) filtrate offer a safer and more eco-friendly alternative. One factor affecting anthocyanin color stability is storage duration. This study aimed to evaluate the effectiveness of teak leaf filtrate stored for 0, 7, 14, and 21 days on the staining quality of STH egg preparations. This quasi-experimental study used a Posttest-Only Nonequivalent Control Group Design. The samples consisted of 24 Ascaris lumbricoides egg preparations divided into four treatment groups with six replications each. Staining quality was assessed by three validators based on contrast under the microscope, color absorption, and morphological clarity. Data were analyzed using the Kruskal-Wallis test to determine differences in staining quality across storage durations. All preparations showed good staining quality, and no significant differences were found between the storage groups (p-value = 0.383; > 0.05). These results indicate that teak leaf filtrate remains effective for staining STH egg preparations for  up to 21 days of storage, with the best results observed at 7 days after preparation. Further research is recommended to explore this topic more deeply, with attention to laboratory procedures and technical skills.