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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Veteriner Prosiding Seminar Biologi Jurnal Kedokteran Brawijaya BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Biomedical Engineering Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL BIOLOGI INDONESIA Jurnal Penelitian Sains PROSIDING SEMINAR NASIONAL Jurnal Biomedika dan Kesehatan Indonesian Journal of Mollusk Proceeding Biology Education Conference Jurnal Kedokteran Hewan Berkala Ilmiah Biologi
Niken Satuti Nur Handayani
Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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Journal : Jurnal Kedokteran Hewan

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IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis Utami, Prasetyarti; Handayani, Niken Satuti Nur; Kunda, Rony Marsyal
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.2960

Abstract

ABSTRACTThis study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.
Co-Authors Andika Tripramudya Onggo Anita Widianawati, Anita Aurantika, Rindu BUDI SETIADI DARYONO Dian Pratiwi, Arini Feri Sukmawati Ganies Riza Aristya Harahap, Nur Imma Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hery Wijayanto Hidayat, Madyan Akmal Indra Lesmana Karmilah, Karmilah Kazuo Yoshida Kazuo Yoshida, Kazuo Laksono Trisnantoro Latifah, Vida Rahma Maria Natalina Lumban Gaol Mukhlis Mukhlis Mulyadi Mulyadi Mulyati Mulyati Nainggolan, Ananto Puradi Nobukazu Tanaka Nobukazu Tanaka, Nobukazu Nugraheni, Kireida Asta Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan P, Purnomo Pertiwi, Ivory Martha Pratiwi, Arini Dian Priyambodo Priyambodo Rarastoeti Pratiwi Rini Widayanti Rini Widayanti Rony Marsyal Kunda Rury Eprilurahman Saefudin, Siti Amalia Firdza Thoifur Ibnu Fajar, Muhammad Tony Febri Qurniawan Tri Ratnaningsih Trijoko Trijoko (Trijoko) Tuty Arisuryanti, Tuty Utami, Prasetyarti Widyasari, Adristi Shafa Yusuf, Adib Fakhruddin Yuyun Machwiyah Yuyun Machwiyah