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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Veteriner Prosiding Seminar Biologi Jurnal Kedokteran Brawijaya BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Biomedical Engineering Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL BIOLOGI INDONESIA Jurnal Penelitian Sains PROSIDING SEMINAR NASIONAL Jurnal Biomedika dan Kesehatan Indonesian Journal of Mollusk Proceeding Biology Education Conference Jurnal Kedokteran Hewan Berkala Ilmiah Biologi
Niken Satuti Nur Handayani
Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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Journal : Jurnal Veteriner

 1 
Keragaman Genetik Gen Penyandi Dehydrogenase Sub-unit 3 Mitokondria pada Monyet Hantu (Tarsius sp.) (GENETIC DIVERSITY OF MITOCHONDRIAL DEHYDROGENASE SUB-UNIT 3 GENE ON TARSIUS SP.) Rini Widayanti; Niken Satuti Nur Handayani; I Made Budiarsa
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tarsius is an endemic species in Indonesia but is is getting threatened. In-situ and ex-situ conservationof this species would yield better results when its genetic information and diversity determined. Theobjective of this ressearch was to study the genetic diversity of ND3 gene of Tarsius sp. Based on sequencingof PCR products using primer ND3F and ND3R resulted in 437 nts base sequence. Results of ND3 fragmentssequencing were put on multiple alignment with other primates from Genbank using of software ClustalW, and then were analyzed using MEGA program version 4.1. A different nucleotide site was found(nucleotide no. 24). The genetic distance based on nucleotide ND3 was calculated using Kimura 2-parametermodel. The genetic distance showed the smallest genetic distance 0%, the biggest 0,03% and average0,01%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide ND3 genecould not be used to differentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from NorthSulawesi) and T. bancanus (from Lampung, South Sumatera).
Keragaman Genetik Sekuen Gen ATP Synthase FO Subunit 6 (ATP6) Monyet Hantu (Tarsius) Indonesia (GENETIC DIVERSITY STUDY OF ATP6 GENE SEQUENCES OF TARSIERS FROM INDONESIA) Rini Widayanti; Niken Satuti Nur Handayani; Hery Wijayanto
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In a conservation effort, the identification of Tarsier species, on the bases of the morphological andmolecular characteristic is necessary. Up to now, the identification of the animals were based on themorphology and vocalizations, which is extremely difficult to identify each, tarsier species. The objective ofthis research was to study the genetic diversity on ATP6 gene of Tarsius sp. Based on sequencing of PCRproduct using primer ATP6F and ATP6R with 681 nts. PCR product. The sequence of ATP6 fragmentswere aligned with other primates from Gene bank with aid of software Clustal W, and were analyzed usingMEGA program version 4.0. Three different nucleotide sites were found (nucleotide no. 288, 321 and 367).The genetic distance based on nucleotide ATP6 sequence calculated using Kimura 2-parameter modelindicated that the smallest genetic distance 0%, biggest 0.8% and average 0, 2%. The phylogenetic treeusing neighbor joining method based on the sequence of nucleotide ATP6 gene could not be used todifferentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from North Sulawesi), T. bancanus(from lampung, South Sumatera) and T.bancanus from West Kalimantan.
Co-Authors Andika Tripramudya Onggo Anita Widianawati, Anita Aurantika, Rindu BUDI SETIADI DARYONO Dian Pratiwi, Arini Feri Sukmawati Ganies Riza Aristya Harahap, Nur Imma Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hery Wijayanto Hidayat, Madyan Akmal Indra Lesmana Karmilah, Karmilah Kazuo Yoshida Kazuo Yoshida, Kazuo Laksono Trisnantoro Latifah, Vida Rahma Maria Natalina Lumban Gaol Mukhlis Mukhlis Mulyadi Mulyadi Mulyati Mulyati Nainggolan, Ananto Puradi Nobukazu Tanaka Nobukazu Tanaka, Nobukazu Nugraheni, Kireida Asta Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan P, Purnomo Pertiwi, Ivory Martha Pratiwi, Arini Dian Priyambodo Priyambodo Rarastoeti Pratiwi Rini Widayanti Rini Widayanti Rony Marsyal Kunda Rury Eprilurahman Saefudin, Siti Amalia Firdza Thoifur Ibnu Fajar, Muhammad Tony Febri Qurniawan Tri Ratnaningsih Trijoko Trijoko (Trijoko) Tuty Arisuryanti, Tuty Utami, Prasetyarti Widyasari, Adristi Shafa Yusuf, Adib Fakhruddin Yuyun Machwiyah Yuyun Machwiyah