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Journal : Jurnal Perikanan dan Kelautan

 1 
Antioxidant Activity of Bioactive Peptides on Protein Hydrolyze of Bombay Duck (Harpodon nehereus) from Papain Hydrolysis Rahman, R. Fathul; Buchari, Dewita; Hasan, Bustari
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aimed to examine the antioxidant activity of bioactive peptides on protein hydrolyzes of Bombay duck (Harpodon nehereus)from hydrolysis using papain enzyme. This research used an experimental method, with a wholly randomized non-factorial design consisting of enzyme concentration factors (1,5, 2, and 2,5%). Parameters tested include chemical composition tests of Bombay duck's meat, flour, and protein hydrolyze, identification of short peptide chains, and antioxidant activity. The results showed that Bombay duck protein hydrolyzes with papain enzyme concentration. There was a significant difference, where the enzyme concentration of 2,5% has the highest degree of hydrolysis of 69,18%. The chemical composition of meat was water 73.15% (ww), protein 81.97% (dw), ash 5.57% (dw), and fat 4,49% (dw). The chemical composition of flour Bombay duck was water 8.32% (ww), protein 79.88% (dw), ash 4,89% (dw), and fat 3,41% (dw). The best treatment of Bombay duck protein hydrolyze at an enzyme concentration of 2.5% resulted in a chemical composition of water 6.73% (ww), protein 80,56 % (dw), ash 5,53 % (dw) and fat 3,11 % (dw). The results of the identification of peptide compounds were L-Pro-L-Ile, L-Leu-L-pro, L-Arg-D-Pro, Leu-Ala-Arg-Leu, and L-Thr-L-pro-L-lys. Furthermore, DPPH free radicals (IC50) scavenging power was 5.19%.
Functional Groups of Scale Collagen from Farmed Snakehead Fish (Channa striata) and Wild Sources Agustina, Ayu; Hasan, Bustari; Sukmiwati, Mery
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The study aimed to analyze the functional groups of scale collagen from farmed snakehead fish and wild sources. Scale collagen from farmed snakehead fish was higher than the wild sources (18.82 > 14.27%) with the protein content of both successively (60.24 > 59.78%). The functional groups in scale collagen from farmed snakehead fish were Amide A (3668,77 cm-1), Amide B (2920,35 cm-1) Amide I (2302,14 cm-1), Amide II (1446,67 cm-1), and Amide III (1245,10 cm-1). Meanwhile, scale collagen from wild sources snakehead fish has an Amide A group (3853.94 cm-1), Amide B (2905.89 cm-1), and Amide I (1869.10 cm-1) Amide II (1457.28 cm-1), Amide III (1243.18 cm-1). Scale Collagen from farmed snakehead fish and wild sources is classified as type 1 collagen.
Antioxidant Activity of Bioactive Peptides on Protein Hydrolyze of Bombay Duck (Harpodon nehereus) from Papain Hydrolysis Rahman, R. Fathul; Buchari, Dewita; Hasan, Bustari
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aimed to examine the antioxidant activity of bioactive peptides on protein hydrolyzes of Bombay duck (Harpodon nehereus)from hydrolysis using papain enzyme. This research used an experimental method, with a wholly randomized non-factorial design consisting of enzyme concentration factors (1,5, 2, and 2,5%). Parameters tested include chemical composition tests of Bombay duck's meat, flour, and protein hydrolyze, identification of short peptide chains, and antioxidant activity. The results showed that Bombay duck protein hydrolyzes with papain enzyme concentration. There was a significant difference, where the enzyme concentration of 2,5% has the highest degree of hydrolysis of 69,18%. The chemical composition of meat was water 73.15% (ww), protein 81.97% (dw), ash 5.57% (dw), and fat 4,49% (dw). The chemical composition of flour Bombay duck was water 8.32% (ww), protein 79.88% (dw), ash 4,89% (dw), and fat 3,41% (dw). The best treatment of Bombay duck protein hydrolyze at an enzyme concentration of 2.5% resulted in a chemical composition of water 6.73% (ww), protein 80,56 % (dw), ash 5,53 % (dw) and fat 3,11 % (dw). The results of the identification of peptide compounds were L-Pro-L-Ile, L-Leu-L-pro, L-Arg-D-Pro, Leu-Ala-Arg-Leu, and L-Thr-L-pro-L-lys. Furthermore, DPPH free radicals (IC50) scavenging power was 5.19%.
Functional Groups of Scale Collagen from Farmed Snakehead Fish (Channa striata) and Wild Sources Agustina, Ayu; Hasan, Bustari; Sukmiwati, Mery
Jurnal Perikanan dan Kelautan Vol. 28 No. 2 (2023): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The study aimed to analyze the functional groups of scale collagen from farmed snakehead fish and wild sources. Scale collagen from farmed snakehead fish was higher than the wild sources (18.82 > 14.27%) with the protein content of both successively (60.24 > 59.78%). The functional groups in scale collagen from farmed snakehead fish were Amide A (3668,77 cm-1), Amide B (2920,35 cm-1) Amide I (2302,14 cm-1), Amide II (1446,67 cm-1), and Amide III (1245,10 cm-1). Meanwhile, scale collagen from wild sources snakehead fish has an Amide A group (3853.94 cm-1), Amide B (2905.89 cm-1), and Amide I (1869.10 cm-1) Amide II (1457.28 cm-1), Amide III (1243.18 cm-1). Scale Collagen from farmed snakehead fish and wild sources is classified as type 1 collagen
Co-Authors ', Desmelati ', Sahyudi ', Sakinah Abdul Salam Badji Adi Putra Silaban Aditya Rachman Aliunputra Alfajriey, Rayhand Aminah Abdullah Anak Agung Istri Sri Wiadnyani Ananda, Dea Andhra Syatapahana Ansyor Refinal Fadilah Nasution Ayu Agustina Ayu Agustina AYU WULANDARI Azura, Asyifa Siti Bella Kharisma Berkhaty, Ayang Putri Dahlia Dahlia Damayani, Noventa Dwi Debi Rachmadani Dedi Iskandar Deevrizal Deevrizal Desmelati ' Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desnizarianti ' Dewita Dewita Dian Iriani Dya Surya Rantika Era Yuliana Evrin Septyanti Fajar Syukron Febriana, Tika Feliatra Fitra Suzanti Ghazali, Tengku Muhammad Giffar Ali Firmansyah Gusti Putra Yunius Guswanda Putra Henry Robby Mustaqim Hutagaol, Debby Alvionita Ilham Azhari Nasution Indra Anggraini Ira Sari Irani, Dian Islami Hakim Karnila, Rahman Karnila Kembaren, Bella Billiant J Ananta M Andesba Arifin M Azwar M. Irvansyah, M. Irvansyah Malau, Chandra Yustitia Maskilin, Jippo Maulani, Iffah Mery Sukmiwati Mery Sukmiwati Mohd. Khan A. N Ira Sari N Ira Sari N. Ira Sari N. Ira Sari Nalurita Nalurita Nasib Naibaho, Nasib Nittaya Chaiyanate, Nittaya Nur Wahid, Nur Nurma Yunita Nurul Atasyah Atasyah Putra, Harifa Syah Putra, Muhammad Ridho Putri Alvin, Okthavia Putri, Desrili Niatami Qalbi Salim R Fathul Rahman Rahman, R. Fathul Ramadhan, Tengku Rahmat Reinaldy Pangestu Riana Silalahi Rika Sepia Alvianti Rinanda, Khairunnisa Yulia Rozi Rudy Irawan Ryan Nazmi Sahliana, Sahliana Sahyudi Sahyudi Santa Monika Santhy Wisuda Sidauruk Sastiwi Sastiwi Septyanti, Elvrin Shandy Shandy Sidik, Fawwaz Afifah Sihombing, Kevin Andreas Sri Aryati Rukmana Nasution Stefani Rani Audina Sukirno Mus Sumarto Sumarto Sumarto Sumarto Sumarto, Sumarto Syahrul Syahrul Tia Sundari Titania Tjandrawati Nugroho Tjipto Leksono Tjipto Leksono Tomy Andri Zulpa Trisla Warningsih Umi Habibah W Sidauruk, Santhy Yustiani, Maulida Zein, Fauzan Fadilah