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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Hemera Zoa Media Veteriner Jurnal Veteriner Current Biochemistry REKAYASA JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Pengetahuan dan Teknologi (IPTEK)
Fachriyan Hasmi Pasaribu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Electrical & Electronics Engineering Engineering Industrial & Manufacturing Engineering Physics Veterinary

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Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER Hapsari Mahatmi; Agus Setiyono; Retno Damayanti Soejoedono; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.
Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS) Iwan Harjono Utama; Aisjah Girindra; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan; Endhie D. Setiawan; Gatut Ashadi; Aida Louise Tenden Rompis
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS)
Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.
Preparasi Imunoglobulin G Kelinci sebagai Antigen Penginduksi Antibodi Spesifik Terhadap Virus Avian Influenza H5N1 Strain Legok Ketut Karuni Nyanakumari Natih; Retno Damayanti Soejoedono; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to prepare rabbit Immunoglobulin G as anti-idiotype antibody (Ab2) ofAvian Influenza Virus (AIV) H5N1. A polyclonal antibody was collected from guinea pigs immunized withinactivated AI vaccine H5N1of Legok strain. Antibody of H5N1 AI in serum was detected by Agar gelprecipitation test (AGPT) and an Inhibition Hemmaglutination test (IHT). The highest titre of antibodywas obtained one week after the third immunization. Serum of guinea pigs containing IgG was purifiedusing the Montage Antibody purification kit & spin column with Prosep A media (Millipore). The AI H5N1IgG concentration was 8 mg/ml. AI H5N1 IgG, was then digested with pepsin to obtain F(ab)2 fraction andwas called Ab1. The concentration of IgG and F(ab)2 and purity of IgG were determined by UVspectrophotometer which showed Ab1 concentration 1 mg/ml. Molecular weight was estimated by sodiumdodecyl sulfate- polyacrilamide gel electrophoresis (SDS-PAGE). Ab2 was produced by immunization ofrabbit with Ab1. The first immunization was carried out by subcutaneous injection with 500 ?g of Ab1emulsified in Complete Freund Adjuvant. The immunization was repeated with the same dose of Ab1emulsified in Incomplete Freund Adjuvan at 1 week intervals. One week after the second immunization,rabbit’s serum was harvested and IgG was purified using the Montage Antibody purification kit & spincolumn with Prosep A media (Millipore). The rabbit IgG, called Ab2, was an anti-idiotypic antibody againstAIV-H5N1. In AGPT, a precipitation line appeared between Ab1 and Ab2. A partial reaction appearedbetween Ab2 and the AI H5N1 antigen was also detected. The results indicated that Ab2 is a possiblecandidate of imunogen for protection against an AI virus H5N1 infection.
Cross Reaction of Serum in Salmonella enteritidis- Vaccinated Chicken to Some Salmonella enterica Serotypes (REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) Wyanda Arnafia; Siti Gusti Ningrum; Erfiandini Eka Puspita; Denny Widaya Lukman; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Salmonella spp. has been recognized as the major cause of food-borne illness in humans worldwidecausing remain relevant to public health. Poultry vaccination is one promising strategy to mitigateSalmonella infection in poultry and, in turn, in humans as well. The objective of this study was to assessthe potential of cross-reaction of serum in Salmonella enteritidis-vaccinated chicken to some serotype ofSalmonella enterica. Four female, Isa Brown layer chickens (20 weeks old), were vaccinated with S. enteritidisstrain Sm24/Rif12/Ssq (intra vena) to induced the production of specific antibodies in serum. Crossreactionof serum in S. enteritidis-vaccinated chicken were assess with agar gel immunodiffusion test(AGID) with S. enteritidis, S. pullorum, S. typhimurium, S. typhi, and Escherichia coli antigens. Serumcould react with S. enteritidis and all types of S enterica used in this study (S. pullorum, S. typhimurium,S. typhi), but could not react with E. coli. The potential of cross-reaction of serum in S. enteritidis-vaccinatedchicken to some serotypes of S. enterica may play a role in reducing the infection caused by that serotype.
Aktivitas Antibakteri Isolat Bakteri Endofit dari Tumbuhan Nyawai (Ficus variegata Blume) Shinta Leonita; Maria Bintang; Fachriyan Hasmi Pasaribu
JURNAL ILMU PENGETAHUAN DAN TEKNOLOGI (IPTEK) Vol. 2 No. 1 (2018): Jurnal Ilmu Pengetahuan dan Teknologi (IPTEK)
Publisher : Institut Teknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31543/jii.v2i1.133

Abstract

Bakteri endofit menjadi salah satu alternatif penghasil senyawa antibakteri. Keberadaannya pada tumbuhan berkhasiat obat salah satunya tumbuhan nyawai (Ficus variegata Blume) memungkinkan memproduksi senyawa bioaktif seperti yang terkandung oleh tumbuhan inangnya. Ekstraksi senyawa bioaktif dari tanaman dinilai tidak efisien sebab memerlukan biomassa yang besar, sehingga  cara yang dapat digunakan adalah dengan memanfaatkan bakteri endofit. Penelitian ini bertujuan mengisolasi dan menguji aktivitas antibakteri isolat bakteri endofit dari tumbuhan nyawai terhadap Eschericia coli, Staphylococcus aureus, Bacillus subtilis dan Pseudomonas aureus. Sebanyak 29 isolat bakteri endofit berhasil diisolasi dari tumbuhan nyawai. Berdasarkan uji antibakteri sebanyak 5 isolat bakteri endofit mampu menghambat pertumbuhan E. Coli, 6 isolat mampu menghambat S. aureus, 4 isolat mampu menghambat B. subtilis dan 4 isolat mampu menghambat P. aeruginosa. Kata Kunci : antibakteri, bakteri endofit, Ficus variegata Blume
Resistance of Klebsiella Sp. Isolated From Chicken and Cages to Chloramphenicol Rahmat Hidayat; Fachriyan Hasmi Pasaribu; Sri Mulatsih; Akhmad Arif Amin
Rekayasa Vol 16, No 2: Agustus 2023
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v16i2.20277

Abstract

Klebsiella bacteria is one of the normal flora in poultry that has developed antibiotic resistance. This is reinforced by reports of the incidence of resistance by Klebsiella bacteria in various countries which is quite high. This study aimed to identify the presence of Klebsiella sp. bacteria in chickens and cages, as well as their resistance status to chloramphenicol in relation to the Minister of Agriculture Regulation No. 14/2017. The series of identification included culture, macroscopic and microscopic observations, biochemical tests, and fermentation tests. Antibiotic resistance tests using chloramphenicol disks referring to the Kirby Bauer method. The identification results showed 6 positive samples from 60 samples taken from chickens and 2 positive samples from 21 samples taken from cages. Resistance testing of 8 Klebsiella sp. isolates showed 6 sensitive, 1 intermediate and 1 resistant isolates to chloramphenicol. This is an indication that the Minister of Agriculture Regulation No. 14/2017 is quite effective in prohibiting the use of chloramphenicol in chicken farms.
Characterization and molecular detection of pathogenicity and antibiotic resistance genes in Vibrio parahaemolyticus isolated from Pacific white shrimp Muhammad Arif Mulya; Fachriyan Hasmi Pasaribu; Usamah Afiff; Munti Yuhana
Jurnal Akuakultur Indonesia Vol. 21 No. 1 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.1.81-92

Abstract

ABSTRACT This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus ABSTRAK Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus
Co-Authors Afiff , Usamah Agus Setiyono Agustin Indrawati Aida Lousie Tenden Rompis Aisjah Girindra Anak Agung Sagung Kendran Aprilia Hardiati Denny Widaya Lukman Dwi Endah Kusumawati Endhie D. Setiawan Erfiandini Eka Puspita Gatut Ashadi Hapsari Mahatmi I wayan Teguh Wibawan Iwan Harjono Utama Ketut Karuni Nyanakumari Natih MARIA BINTANG Muhammad Arif Mulya Muharam Saepulloh MUNTI YUHANA Nabila Swarna Puspa Hermana Nampiah Sukarno Purwanto, Ukhradiya Magharaniq Safira Rahmat Hidayat Retno Damayanti Soejoedono Riza Zainuddin Ahmad Safika S, Safika Shinta Leonita Siti Gusti Ningrum Sri Mulatsih Trioso Purnawarman Triwardhani Cahyaningsih Wyanda Arnafia Yaddi, Yafsin Zumala Nilasari