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THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Effect of Cold Storage Duration on the Quality of Super Red (Hylocerus costaricensis) and White (Hylocerus undatus) Dragon Fruits Latif, Fahmi Nur Aida; Nurchayati, Yulita; Hastuti, Endah Dwi
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 1 (2025): February 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i1.39-48

Abstract

Postharvest storage is carried out to control the metabolic rate so the quality of fruits can be maintained properly until it reaches the costumers. Cold storage can reduce metabolic reactions, such as respiration and transpiration. However, storing too long in cold temperatures can cause the quality of the fruit to deteriorate. Each type of dragon fruit has different chemical contents that will affect the quality of fruits after storage. This study aims to determine the effect of the type of dragon fruit, cold storage duration, and interaction between the two treatments. Both type of dragon fruit are packed in PP plastic and then stored at 10°C. Observation were made at different storage durations. This study used RAL, with the first being super red and white dragon fruits. The second factor was storage duration of 0, 7, 14, and 21 days. Data were analyzed using ANOVA and continued with DMRT if it had a significant effect. The result of the study showed that the treatment of fruit type had significant effect on antioxidant activity, vitamin C content, and total sugar level. Storage duration treatment had significant effect on antioxidant activity and vitamin C content. Keywords: Antioxidant activity, Cold storage, Dragon fruit, Post-harvest, Vitamin c.
The Physiological Response of Germination and Growth in Solanaceae Plants (Capsicum frutescens, Solanum melongena, Solanum lycopersicum) at Different Salinity Levels Della, Nelly Vikiladyla; Hastuti, Endah Dwi; Setiari, Nintya
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1352-1360

Abstract

High salinity causes osmotic stress and ion imbalance that can reduce plant productivity. Solanaceae can be developed for cultivation in saline land, but its growth is influenced by the type of species. This study aims to examine the tolerance level of three Solanaceae plants to salinity stress through observation of physiological responses of germination and growth. This study used a 3 x 4 factorial Completely Randomized Design (CRD). The first factor is salinity: 0 ppm, 2,500 ppm, 5,000 ppm and 7,500 ppm. The second factor is the Solanaceae species, namely Capsicum frutescens, Solanum melongena, and Solanum lycopersicum. Germination parameters include germination power, wet weight and dry weight. The growth parameters observed include plant height, root length, stem diameter, leaf area, number of leaves, wet weight of leaves, roots and stems and dry weight of leaves, roots and stems. The results of the study showed that C. frutescens is a plant that is more tolerant to salinity up to a concentration of 5,000 ppm when compared to S. melongena and S. lycopersicum whose tolerance is up to 2,500 ppm. Keywords: Germination, Salinity, Solanaceae, Vegetative Growth.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
POTENTIAL OF MANGROVE SEEDLINGS FOR UTILIZATION IN THE MAINTENANCE OF ENVIRONMENTAL QUALITY WITHIN SILVOFISHERY PONDS Hastuti, Endah Dwi; Budihastuti, Rini
BIOTROPIA Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.248 KB) | DOI: 10.11598/btb.2016.23.1.606

Abstract

Silvofishery system has been applied to aquaculture activities and it has been developed in the coastal area of Semarang City, Indonesia. However, information on the initial development of silvofishery ponds concerning the functionality of mangrove seedlings on environmental quality of fish ponds had not been studied. This experiment aimed to determine the environmental conditions of silvofishery ponds and to analyze the effect of seedling stands of mangrove on environmental quality control. The presence of mangrove seedlings caused the decrease of temperature and the increase of salinity. ANOVA showed that mangrove species significantly affected water salinity, while canal width and mangrove species significantly affected turbidity and pH. Regression analysis showed that the height of Rhizophora mucronata had partially significant effect on Total Suspended Solids (TSS), Organic Matter (OM), as well as Nitrogen (N) and Phosphorus (P) concentrations. Diameter of R. mucronata affected temperature. The height and diameter of Avicennia marina affected Dissolved Oxygen (DO). Mixed populations of A. marina and R. mucronata had an effect on water turbidity, while population of only A. marina had a partial effect on water salinity. R. mucronata seedlings had dominant effect on the environmental quality. Mangrove seedlings can be used as environmental quality control within silvofishery ponds to maintain optimal conditions for fish growth. The application of silvofishery in early stage of mangrove seedlings should consider the more abundant plantation of R. mucronata compared to A. marina.
INCREASING RISK OF HEAVY METAL CONTAMINATION IN SILVOFISHERY PONDS Hastuti, Endah Dwi
BIOTROPIA Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.6 KB) | DOI: 10.11598/btb.0.0.0.983

Abstract

This research aimed to determine the concentration of organic matter, Pb, and Cd found in a silvofishery pond, to assess the toxicity level, to analyze changes in their concentrations within a one-year period, and to analyze the correlation between these concentrations and their changes. The research was conducted through five field observation activities and laboratory analyses from May 2016 to July 2017. Data were analyzed using ANOVA and correlation tests. The concentration of organic matter, Pb, and Cd increased across all five observations. Throughout the research, the concentration ranges were 1.60–3.30 mg/kg for organic matter, 3.130–8.230 mg/kg for Pb, and 1.089–2.820 mg/kg for Cd. In all observations, the toxicity assessment showed that Cd concentration in the sediment exceeded the standards recommended by the US EPA (≤1.0 mg/kg) and ANZECC & ARMCANZ (≤1.5 mg/kg), while Pb concentration remained within the safe limits (≤21 mg/kg and ≤50 mg/kg, respectively). Moreover, the concentration and accumulation of Pb and Cd were strongly correlated, indicating the possibility of originating from the same pollutant sources. Therefore, a better management plan is recommended to prevent heavy metal accumulation in silvofishery ponds, including arranging mangrove plants along inlet canals and implementing periodic pruning to prevent heavy metals from re-entering the environment through litter fall.