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Journal : Folia Medica Indonesiana

Effects of Arak Bali Administration on Spermatozoa DNA Fragmentation and Testosterone Level of Rats (Rattus norvegicus) Alfiah Hayati; Dwi Winarni; Ni Wayan Sukma Antari; Inne Ninda Resita; Erlyn Nurul Fauziah
Folia Medica Indonesiana Vol. 54 No. 1 (2018): March
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.883 KB) | DOI: 10.20473/fmi.v54i1.8051

Abstract

This study aimed to determine the effects of arak bali on the fragmentation of spermatozoa and testosterone in rats (Rattus norvegicus). This study used 24 rats (170-200 g), divided into four groups: one control and three treatments (receiving arak bali containing 40% alcohol as much as 0.1 and 0.5 mL and synthetic alcohol (40%) as much as 0.1 mL, for 45 days). The observation of DNA fragmentation was done using acridine orange staining and the measurement of testosterone level used ELISA method. The results showed that the provision of arak bali in experimental animals increased the occurrence of spermatozoa DNA fragmentation. The higher the volume of arak bali given, the higher the fragmentation of spermatozoa DNA. The administration of arak bali also decreased testosterone level. The higher the volume of arak bali given, the lower the rats' testosterone level.
Effects of Red Fruit (Pandanus conoideus Lam) Oil on Malondialdehyde Level and Spermatozoa Quality in Mice (Mus musculus) Exposed to Monosodium Glutamate Widayati Agustina; Widjiati Widjiati; Alfiah Hayati
Folia Medica Indonesiana Vol. 54 No. 2 (2018): June
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.602 KB) | DOI: 10.20473/fmi.v54i2.8855

Abstract

This study aimed to determine the effects of red fruit (Pandanus conoideus Lam) oil on MDA levels and spermatozoa quality in mice (Mus musculus) exposed to MSG. The quality includes motility, viability, concentration, and morphology of spermatozoa. This experimental study used randomized post-test only control group design. The subjects of this study were 25 mice (Mus musculus), divided into 5 groups (5 mice per group). K- group received distilled water for 35 days. K+ group received 4 mg/g BW MSG for 21 days. P1, P2, and P3 treatment groups received 4 mg/g BW MSG for 21 days and 0.02; 0.04; 0.08 ml/g BW red fruit oil, respectively, from day 22 to 35. The results showed that mean spermatozoa morphology in K-, K+, P1, P2, P3 groups were as follows: 0.86; 0.56; 0.67; 0.61; and 0.87 (%). The spermatozoa concentrations were sequentially as follows: 21; 10; 15; 32,8,19 (107 cells/ml). The spermatozoa's vitalities were as follows: 0,64; 0,14; 0,24; P2: 0.36; 0.68 (%). MDA levels were respectively: 0.29; 0.60; 0.35; 0.23; and 0.19 (nm). As a conclusion, testicular MDA levels in mice exposed to MSG and given with red fruit oil were lower than those in mice exposed to MSG without receiving red fruit oil. The quality of spermatozoa in mice exposed to MSG and receiving red fruit oil was higher than that of mice exposed to MSG without being given with red fruit oil.
PROVISION OF ARAK BALI REDUCES SPERMATOZOA QUALITY OF WHITE RATS (Rattus norvegicus) Sukma Antari, Ni Wayan; Hayati, Alfiah; Winarni, Dwi
Folia Medica Indonesiana Vol. 52 No. 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.306 KB) | DOI: 10.20473/fmi.v52i4.5468

Abstract

This study aimed to determine the effect of arak bali on the quality of spermatozoa include morphology, motility, viability, membrane integrity of spermatozoa rat (Rattus norvegicus). The study was conducted in two phases: the first phase of the deployment of questionnaires conducted in five districts in Bali to determine the type and frequency of arak bali consumption and phase II made arak bali giving treatment in experimental animals. This study used 24 male rats (170-200 grams), divided into four groups: one control and three treatments (by arak bali containing 40% alcohol as much as 0.1 and 0.5 mL and 0.1 mL much alcohol synthesis, for 45 days. the results showed that of the five districts in Bali, most people consume arak bali commercial and most of the frequency of consumption of the week more than one bottle (350 mL). the provision of arak bali in experimental animals, degrade the quality (morphology, motility, viability, membrane integrity), the greater the volume given declining spermatozoa quality.
Co-Authors Adelah Putri Milenia Sari Affandi, Moch Agoes Soegianto Agustina, Widayati Amin, M. Hilman F. Analisa, Izza Analisa, Nur Izzah Ansori, Arif NM. Antien Rekyan Seta Aucky Hinting Aunurohim Aurira Thrisna Dwi Aprianti Azizaturrohmah Bayyinatul Muchtaromah Berlian Rustantina Binti Mar`atus Solikha Demircan, Turan Dewi, Firli RP. Dewi, Putri Alicia Duhita, Maharani R. Dwi Winarni Ely Nuril Fajriyah Erlyn Nurul Fauziah Erna Agustina Fajriyah, Ely Nuril Farah Annisa Nurbani Fatma Aulia Nadiyah Fatmi Indah Hati Firdaus, Ana MK. Firli Rahmah Primula Dewi Gebby Agnessya Esa Oktavia Habibi, Soraya Hana Widyana Hanik Faizah I.B. Rai Pidada Idris, Muhammad F. Iman Dary Supriyadi Putra Inesavira Rindaputri Inne Ninda Resita Jamil, Arniza KM. Khaleyla, Firas Khusnita Giarti Ladiya Fitri Ma’rufah Laili Musyarofah Lestari, Lita I.D. Litazkiyyah, Litazkiyyah Manikya Pramudya Maya Safitri Maya Safitri Minarno, Eko B. Muhamad Fadhil Mirza Muhamad Hilman Fu'adil Muhamad Maulana Abdizen MUHAMMAD FAHMI Muhammad Hilman Fu'adil Amin Mujahidin Ahmad Nabilla, Nithasya Nashrulloh, Allam Thoriq Nazil Dwi Rahayuningtyas Ni Wayan Sukma Antari Nina Puspita Ninik Fadhillah Nita Yuliarini Nur Izzah Analisa Nurbani, Farah Annisa Nureka Tiantono Nuris Auliya Nurul Mahmudati Prilya Dewi Fitriasari Princessa Nandita Febrionny Risanda Ulinnuha Rohmatika, Aulia U. Rosmanida Rosyidah, A'liyatur S. Sugiharto Septiani, Adiibtia Setiyowati, Putri Ayu Ika Soesanto Mangkoewidjojo Soraya Habibi Sri Puji Astuti Wahyuningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sugiharto Suhestri Suryaningsih Sukarti Moeljopawiro Trisnadi Widyaleksono Catur Putranto Umi Nur Alfiah Ummi Zubaidah Virid Gibson, Virid Vuanghao Lim Vuanghao, Lim Widi Pangestu Wilujeng Widjiati Widjiati Yuli Winarsih