Rudi Heryanto
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia; Advanced Research Laboratory, Institute Of Research And Community Services, IPB University, Jl. Palem Kampus

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Phenolics Profiling and Free Radical Scavenging Activity of Annona muricata, Gynura procumbens, and Typhonium flagelliforme Leaves Extract Dewi Anggraini Septaningsih; Amalia Yunita; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.62124

Abstract

The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelliforme (keladi tikus) have been used as traditional medicines in Indonesia. This study aims to determine the antioxidant capacity and putatively identified phenolics from the leaves of three medicinal plants forementioned above. We used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method for measuring radical scavenging (antioxidant assay) while the phenolics profiling was determined using UHPLC-Q-Orbitrap HRMS. The results showed that the percentage of radical scavenging activity of G. procumbens leaves extract in ethanol was higher than the other two plants. Phenolics profiling of the three medicinal plants was identified with 38 compounds belonging to flavones and flavanols hydroxycinnamic acid, and several other groups. The number of metabolites identified putatively was 12, 31, and 19 metabolites in the extracts of A. muricata, G. procumbens, and T. flagelliforme, respectively. The results confirmed the correlation between the phenolics presence and the antioxidant capacity of three plants used in this study.
OPTIMALISASI PEMBERDAYAAN MASYARAKAT DESA SUKAMANAH MENUJU ERA MODERN Rudi Heryanto; Megasari Putri; Dewa Saepurrahman; Jihad Hari; Elsa Ramadanti; Yudi Nata; Dudih Gustian; Rico Sihotang; Debby Rahmawaty; Muhamad Amar; Aan Setiawati
Jurnal Pengabdian Kepada Masyarakat Abdi Putra Vol 1 No 2 (2021): September 2021
Publisher : Universitas Nusa Putra & Persatuan Insinyur Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1193.693 KB) | DOI: 10.52005/abdiputra.v1i2.94

Abstract

Pemberdayaan pengabdian kepada masyarakat merupakan kegiatan wajib bagi seluruh mahasiswa Universitas Nusa Putra dalam menyelesaikan pendidikan sarjana. Pemberdayaan pengabdian kepada masyarakat diharapkan mampu mendorong kontribusi lingkungan pendidikan terhadap perkembangan kemajuan masyarakat. Pengelolaan jalan lingkungan, administrasi pemerintahan desa setempat, tata cara hidup bersih dan penyadaran akan pentingnya pendidikan untuk kesejahteraan generasi mendatang
Metabolite Profiling of Java Turmeric (Curcuma xanthoriza) Essential Oil with Different Harvest Times Mohamad Rafi; Dewi Anggraini Septaningsih; Rudi Heryanto
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2511.972 KB) | DOI: 10.14710/jksa.21.4.237-241

Abstract

Java turmeric (Curcuma xanthoriza) is one of the native plants from Indonesia. Java turmeric is known as a medicinal plant with a high content of volatile oils. In this study, we investigated the chemical profile of java turmeric essential oil obtained by hydrodistillation associated with different harvesting times of the rhizome (7, 9, and 11 months). The constituent of the essential oil was determined by using gas chromatography-mass spectrometer. About 19, 20 and 34 compounds were identified in 7, 9, and 11-month old java turmeric. Fifteen compounds were found in all samples namely β-elemene, zingiberene, γ-elemene, β-farnesene, α-curcumene, benzofuran, α-cedrene, epicurzerenone, ar-curcumene, germacrone, aromadendrene, α–longipene, trans-caryophilene, curcuphenol, and xanthorrhizol. This study showed the level of all compound detected in 7, 9, and 11 months old of java turmeric is different.
Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique Wulan Tri Wahyuni; Rudi Heryanto; Eti Rohaeti; Achmad Fauzi; Budi Riza Putra
Jurnal Kimia Sains dan Aplikasi Vol 24, No 2 (2021): Volume 24 Issue 2 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3442.057 KB) | DOI: 10.14710/jksa.24.2.43-50

Abstract

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.
Pembuatan Recycle Screen Printed Carbon Electrode dan Aplikasinya untuk Deteksi Asam Galat dengan Teknik Voltammetri Wulan Tri Wahyuni; Rany Zalvianita; Rudi Heryanto
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3056.934 KB) | DOI: 10.14710/jksa.22.5.164-172

Abstract

Gallic acid is phenolic compound found in tea and act as antiradical agent. This compound is electrochemically active and could be detected using voltammetric technique. This study aims to obtain recycle screen printed carbon electrode (rSPCE) and applied it for voltammetric detection of gallic acid. rSPCE was prepared by modification of used SPCE working electrode using a mixture of graphite:polystyrene at ratio of 95:5, 90:10, and 85:15 (w/w). Graphite:polystyrene was suspended in chloroform and deposited on SPCE by drop casting method. Phosphate buffer 0.1 M at pH of 2.5, 3.5, and 7 were used as electrolyte solution in voltammetric detection of gallic acid. The optimum condition of gallic acid detection was obtained when phosphate buffer 0,1 M at pH of 2.5 and rSPCE in composition of graphite:polystyrene (95:5) used as electrolyte and working electrode, respectively. Gallic acid has 2 oxidation peaks at potential of 0.26 V and 0.63 V vs Ag/AgCl, respectively. At concentration of 1 – 5 mM, gallic acid and oxidation currents provide linear regression with the coefficient determination of 0.9947 and 0.9864, consecutively for peak number 1 and 2. Measurement of gallic acid at rSPCE 95:5 shows good precision with %RSD < 5%. rSPCE was successfully applied for voltammetric detection of gallic acid in standard solution with accuracy > 96%, however further development is needed before its application for gallic acid measurement in real sample such as tea extract.
Phytochemical Profiling of Gynura procumbens (Lour.) Merr. Leaves and Stem Extracts Using UHPLC-Q-Orbitrap HRMS Dewi Anggraini Septaningsih; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.74236

Abstract

In Indonesia, Gynura procumbens (Lour.) Merr., known as Longevity Spinach or Sambung Nyawa, is commonly grown in tropical and subtropical Asian countries. Many biological activities of G. procumbens have been reported. As we know, the composition and concentration of metabolites, as well as plant parts will significantly affect the biological activities. In this work, UHPLC-Q-Orbitrap-HRMS was used for the putative identification of metabolites present in 70% ethanol extract of G. procumbens leaves and stem extract. Also, we performed clustering of G. procumbens leaves and stem extracts using principal component analysis (PCA) with the peak area of the identified metabolites as the variable. Thirty-one metabolites were identified, and the number of identified peaks in the leaves is higher than in the stem. Those identified metabolites are phenolics, fatty acids, oxo monocarboxylic acids, porphyrins, and chlorophyll fragments. The PCA results showed that the leaves and stem extracts could be grouped, indicating that the composition and concentration of detected compounds differed.
Profil Kimia dan Toksisitas Jamu Berpotensi Antidiabetes yang Diformulasi dengan Metode Statistika dan Machine Learning Norma Nur Azizah; Rudi Heryanto; Wisnu Ananta Kusuma
Jurnal Jamu Indonesia Vol. 3 No. 1 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1327.466 KB) | DOI: 10.29244/jji.v3i1.47

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Bahan alam yang dapat menghambat spermatogenesis merupakan suatu alternatif untuk kontrasepsi pria. Penelitian ini bertujuan mengevaluasi formulasi optimum berbasis biji jarak pagar dan buah pare yang dapat menginhibisi spermatogenesis. Bahan sampel dimaserasi alkohol 70% untuk memperoleh ekstrak kasar. Ekstrak diujikan pada tikus Wistar jantan dewasa sebanyak 30 ekor dan dibagi menjadi enam kelompok secara acak (n=5 ekor). Kelompok I sebagai kontrol memperoleh pelarut akuades; kelompok II dan III secara berurutan memperoleh ekstrak biji jarak dan pare dengan dosis 50 mg/kgBB. Tiga kelompok lainnya, yaitu IV, V, dan VI memperoleh formulasi gabungan dengan rasio ekstrak biji jarak pagar dan pare 1:3, 3:1 serta 1:1, secara berurutan. Perlakuan diberikan per oral satu hari sekali selama 48 hari sesuai dengan siklus spermatogenesis. Pada akhir perlakuan, pemeriksaan sperma untuk konsentrasi dan kualitasnya serta bobot testis. Hasil uji rendemen ekstrak biji jarak pagar dan buah pare secara berurutan sebesar 6.11 % dan 3.32 %. Senyawa fitokimia yang terdapat pada ekstrak pare dan biji jarak pagar antara lain, alkaloid, fenol, flavonoid, tanin, saponin, triterpenoid, dan steroid. Efek ekstrak terhadap bobot testis dan konsentrasi sperma tidak ada perbedaan yang nyata antar kelompok (ANOVA P >0.05) sedangkan untuk kualitas motilitas sperma ada kecenderungan menurun pada formulasi ekstrak biji jarak dan buah pare (1:3).
Spektrofotometer Quali-Vis dan Kemometrika untuk Klasifikasi Kualitas Daun Wungu (Graptophyllum pictum) Silvi Agusri Putri; Rudi Heryanto; Eti Rohaeti
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1584.294 KB) | DOI: 10.29244/jji.v3i3.66

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Spektrofotometer Quali-Vis digunakan untuk mengklasifikasikan 3 sampel daun wungu dengan usia panen berbeda. Penelitian ini bertujuan mengevaluasi model klasifikasi menggunakan spektrofotometer Quali-Vis secara kualitatif. Model dikembangkan dengan akuisisi data menggunakan principle component analysis (PCA) dan partial least square-discriminant analysis (PLS-DA). Karakteristik awal sampel daun wungu ditentukan dengan analisis sidik jari kromatografi lapis tipis (KLT) yang menampilkan profil yang berbeda pada masing-masing usia panen. Perbedaan ini menjadi dasar klasifikasi untuk model PLS-DA yang terdiri atas model usia 1 bulan, 2 bulan, dan 3 bulan, yang menghasilkan rerata R2pred = 0.7794 dan root mean square error predict (RMSEP) = 0.2180. Model divalidasi dengan menentukan nilai parameter sensitivitas, spesifitas, presisi, dan akurasi yang menghasilkan nilai rerata masing-masing 0.95, 0.99, 0.98, dan 0.98 kesesuaian prediksi. Nilai-nilai ini menunjukkan bahwa model yang dikembangkan memiliki kemampuan klasifikasi yang cukup baik.
Effect of Roasting Process on Antioxidant Activity of Carica Seeds Coffee (Carica pubescens) Sukowati, Siski Andini; Arif, Zulhan; Heryanto, Rudi
Indonesian Journal of Chemical Studies Vol. 1 No. 2 (2022): Indones. J. Chem. Stud., December 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (918.459 KB) | DOI: 10.55749/ijcs.v1i2.13

Abstract

Carica seeds (Carica pubescens), one of the diversification of coffee drinks, served as antioxidants. The roasting process generated degradation of secondary metabolic compounds but produced Maillard reactions products (MRPs). The study aimed to determine the effect of the roasting process on the antioxidant activity of Carica coffee beans and its correlation to the Maillard reaction. The samples roasting temperatures were 100–115, 150–165, and 200–215 °C. The samples were analyzed for proximate, total phenolic, antioxidant, and FTIR. The moisture and ash content of dry seeds were according to SNI 01-2907-2008. The total phenolic content was 3.21–8.99 µgGAE/g, with the highest contents at 200–215 °C. The antioxidant activity was 83–459.67 ppm AEAC. The high phenolic content and antioxidant at 200–215 °C were thought to come from MRPs compounds. The FTIR spectra showed stretching of OH, C=C (aromatic), C=N, C=O, and C-OH (phenol). The spectrum intensity weakened at higher temperatures. However, only the nC–H stretching at 200–215 °C became stronger. The Cluster Ward analysis showed resemblance spectra at roasting temperature levels of 100–115 and 150–165 °C. The roasting process affected the antioxidant activity of Carica coffee beans, thereby increasing the roasting temperature level. This condition resulted in higher levels of antioxidants.
A Brief Review on Fabrication of Screen-Printed Carbon Electrode: Materials and Techniques Wahyuni, Wulan Tri; Putra, Budi Riza; Fauzi, Achmad; Ramadhanti, Desi; Rohaeti, Eti; Heryanto, Rudi
Indonesian Journal of Chemical Research Vol 8 No 3 (2021): Edition for January 2021
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2021.8-wul

Abstract

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.