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Journal : agriTECH

Karakteristik Whey Limbah Dangke dan Potensinya Sebagai Produk Minuman dengan Menggunakan Lactobacillus acidophilus FNCC 0051 Fatma Fatma; Soeparno Soeparno; Nurliyani Nurliyani; Chusnul Hidayat; Muhammad Taufik
agriTECH Vol 32, No 4 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.638 KB) | DOI: 10.22146/agritech.9577

Abstract

Dangke whey has not been widely utilized.  Dangke  whey handling is required  for the prevention of environmental pollution, especially  in Enrekang. Evaluating the characteristics of dangke whey is the initial step of dangke  whey handling. Complete data about the dangke whey would be  scientific information for researchers or the public to further the utilization. Nutritional value of dangke whey allows the use of  Lactobacillus acidophilus FNCC 0051 to be processed into  beverage  products. This research objectives were to investigate the characteristics of whey from dangke waste, which were obtained from milk protein separation by using lyophilization of papaya latex, and it also to evaluate the potential of dangke whey as beverage product by using Lactobacillus acidophilus FNCC 0051. A commercial fermented beverages product is used as a comparison, based on the activity  of growth/bacteria numbers, lactic acid and pH. Milk was added with enzyme solution (lyophilized latex) on the level of 0.4 to 1% (v/v). The best level was used for the production of the fermented whey beverage products. Whey was mixed with tapioca level of 0, 0.35, 0.7, 1.05, and 1.4%, and was heated by stirring for 5 minutes at temperature of 70oC. The whey was pasteurized at the temperature of 80oC for 30 minutes. The whey was cooled, and inoculated with L.acidophilus at the level of 1, 3, 5, and 7%, and incubated at 37°C for 8, 12, 16, 18, and 24 hours, respectively. The characteristics of whey dangke were as follows: the solid total was 6.95 ± 0.23%, lactic acid was 0.1 ± 0.003%, fat was 0.2 ± 0.05%, protein was  0.63 ± 0.009%, lactose was 5.08 ± 0.009%, pH was 6.31 ± 0.01, and viscocity was 0.19 ± 0.004 poise. Dangke whey is potential as a beverage products with the addition of  Lactobacillus acidophilus with quality similar to the commercial fermented beverage after incubated for 16 hours, with the addition of inoculum level of 5% and tapioca level of 0.35% based on the activity of growth, lactic acid and pH. ABSTRAKWhey dangke belum banyak dimanfaatkan. Penanganan whey dangke sangat diperlukan untuk pencegahan pencemaran lingkungan khususnya di Kabupaten Enrekang. Evaluasi karakteristik whey dangke merupakan langkah awal penanganan whey dangke. Data yang lengkap tentang karakteristik whey dangke akan menjadi informasi ilmiah bagi para peneliti ataupun masyarakat untuk pemanfaatan whey dangke lebih lanjut. Nutrisi whey memungkinkan penggunaaan Lactobacillus acidophilus FNCC 0051 untuk diolah menjadi produk minuman. Penelitian ini  bertujuan mengetahui karakteristik whey limbah dangke, diperoleh dari pemisahan protein susu dengan menggunakan getah pepaya liofilisasi serta mengetahui potensi whey dangke sebagai produk minuman menggunakan Lactobacillus acidophilus FNCC 0051 dengan menggunakan produk minuman fermentasi komersial sebagai pembanding, berdasarkan aktivitas pertumbuhan/jumlah bakteri, kandungan asam laktat dan pH. Susu ditambahkan larutan enzim (getah liofilisasi) level 0,4 - 1% (v/v), dan level terbaik digunakan untuk  pembuatan produk minuman whey fermentasi. Whey dicampur tepung tapioka  level 0, 0,35., 0,7., 1,05 dan 1,4% dan dipanaskan sambil diaduk selama 5 menit suhu 70oC. Whey selanjutnya di pasteurisasi suhu 80oC selama 30 menit. Setelah dingin diinokulasi L.acidophilus level 1, 3, 5, dan 7% serta diinkubasi  suhu  37oC selama 8, 12, 16, 18 dan 24 jam. Karakteristik whey dangke adalah; total padatan 6,95±0,23%, asam laktat 0,1±0,003%, lemak 0,2±0,05%, protein 0,63± 0,009%, laktosa 5,08 ±0,009%, pH 6,31±0,01 dan viskositas 0,19±0,004 poise. Whey dangke berpotensi sebagai produk minuman dengan penambahan Lactobacillus acidophilus dengan kualitas yang hampir sama dengan produk minuman fermentasi komersial setelah diinkubasi selama 16 jam, penambahan level inokulum 5%  dan penambahan level tapioka 0,35% berdasarkan aktivitas pertumbuhan, asam laktat dan pH.
Characteristics of Red Palm Oil Oleogel Based on Beeswax and Cocoa Butter and Its Application in Red Chocolate Spread Kurniaditya, Viki Hendi; Setiowati, Arima Diah; Hidayat, Chusnul
agriTECH Vol 44, No 4 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.81434

Abstract

Red palm oil (RPO) is a product obtained from crude palm oil with a high carotenoid content. Despite the potential, the susceptibility of the constituent β-carotene to degradation and liquid state at room temperature poses a significant challenge to its use by affecting the texture of the final chocolate spread. Several studies have proposed using the oleo gelation technique to overcome this challenge, which regulates the structure of oil and fats mixture using an oleo gelator. Therefore, this study aims to evaluate the physicochemical characteristics of RPO oleogel produced using an oleo gelator from a beeswax and cocoa butter blend. The best product obtained was then applied to produce a red chocolate spread. The results showed that RPO oleogel obtained at a high beeswax-cocoa butter ratio had higher hardness values, β-carotene content, and melting points. In addition, the crystal microstructure of the product was needle-form with larger crystal sizes, leading to a significant increase in the oil binding capacity. Combining 9% beeswax and 1% cocoa butter (BW9-LK1) was the best formula for producing a chocolate spread. The characteristics of the red chocolate spread obtained from the best oleogel were 61.47 brightness level (L*), 5.81 red intensity (a*), and 57.81 yellowish (b*), with an oil binding capacity of 99.95%. The melting temperature was 31.89-41.67 ºC, indicating that it could melt at body temperature and was stable at room temperature. During 5 weeks of storage, the hardness and β-carotene content were 6.016.21 N and 469.07-302.67 ppm, respectively. Based on these results, the oleo gelator from beeswax and cocoa butter blend improved the hardness, melting points, and β-carotene content of RPO oleogel. This indicated that it potentially could be used as an alternative to produce a carotenoid-rich chocolate spread.
Boiler Ash of Oil Palm Shell as Adsorbent for Lead Adsorption Nurdiansyah, Nurdiansyah; Hidayat, Chusnul; Suroto, Dian Anggraini
agriTECH Vol 45, No 2 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.93514

Abstract

Palm oil shells and fibers are widely used as fuel for factory boiler furnaces. However, boiler ash residue produced is often underutilized. This study aims to explore the use of boiler bottom ash (BBA) as an adsorbent for the adsorption of lead (Pb) and its subsequent application in palm oil mill effluent (POME) purification for water dilution in crude palm oil (CPO) processing. BBA was activated using 0.2, 0.4, 0.6, and 0.8 mol/L potassium hydroxide (KOH) solutions for 24 hours, and the carbonation was conducted at 400°C for 60 minutes. Factors, such as the concentration of KOH for BBA activation, pH levels, adsorption temperature, adsorption kinetics, and the application of the adsorbent in POME purification for water dilution in the CPO processing model, were evaluated. The results showed that the optimal KOH concentration was 0.4 mol/L, as determined by SEM, EDX, and lead adsorption analysis. The maximum adsorbent capacity of approximately 0.43 mg/g was obtained at 50°C and pH 4.6, with an adsorption rate constant of 5.97 per minute. The results also showed that the adsorption process followed the Langmuir model. In addition, the adsorption activation energy and the Arrhenius constant values were -28675.82 J/mol and 0.0001, respectively. The use of POME filtrate for water dilution had no effect on the free fatty acids, water content, impurities, or DOBI (Deterioration Bleachability of Index) in CPO. Lead value showed significant differences in all treatments without dilution. These results indicate that BBA activated with KOH can function as an adsorbent to reduce lead content. POME purified with BBA adsorbent has the potential to be used as diluent water in CPO processing to reduce raw water use and ultimately decrease POME production.
Development of Coconut Protein Concentrate-Xanthan Gum Conjugate by Wet-Dry Heating Method for Red Palm Oil Emulsification Ramadhani, Anis Dwi; Setiowati, Arima Diah; Hidayat, Chusnul
agriTECH Vol 44, No 1 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.76632

Abstract

Protein-polysaccharide conjugation is commonly achieved by wet and dry heating methods. Therefore, this study aimed to produce red palm oil (RPO) emulsifiers by conjugating coconut protein concentrate (CPC) and xanthan gum (XG) through a combination of wet and dry heating method using a cabinet dryer. Several factors, including reaction time (3, 4, 5, 6, and 7 hours), pH (3, 5, 7, 9, and 11), and protein-polysaccharide ratio (1:3, 1:2, 1:1, 2:1, and 3:1) were evaluated for their effect on the Emulsion Activity Index (EAI) and Emulsion Stability Index (ESI). The ability of the obtaining conjugate to emulsify RPO was evaluated, and the results showed that CPC contained 67.40% protein. Reaction time, pH, and protein-XG ratio had a significant effect on EAI and ESI. Meanwhile, optimal conditions for the formation of the CPC-XG conjugate, based on EAI and ESI, were a reaction time of 5 hours, pH 9, and protein-polysaccharide ratio of 2:1. Fourier Transform Infrared (FTIR) analysis showed that the CPC-XG conjugate had a change in absorption at a wavelength number of around 1640 cm -1 , indicating the presence of a Maillard reaction product. Furthermore, the CPC-XG conjugate used in RPO emulsion has a characteristic EAI value of 23.74 m 2 /g, ESI of 271.32 minutes, a droplet size of 790 nm, and a zeta potential of -36.9 mV. These results suggest that the CPC-XG conjugate produced by the wet-dry heating method has the potential for producing stable RPO emulsions.
Effect of Oleogelation Temperature on Physicochemical Properties and Stability of Peanut Oil Oleogel (Arachis hypogaea L.) Wangsa, Chrisnadya Putri; Fitriani, Amalia; Setiowati, Arima Diah; Hidayat, Chusnul
agriTECH Vol 44, No 3 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.78354

Abstract

Oleogelation is a method to transform liquid oil into solid fats without altering the fatty acid profile. Compared to hydrogenation, oleogelation requires a relatively simple process and does not produce trans fats. In oleogelation, temperature has a crucial role in affecting the properties of oleogel. Therefore, this study aimed to examine the effect of oleogelation temperature on the physicochemical properties and stability of peanut oleogel. In this study, peanut oil oleogel was formed at 70 °C, 80 °C, and 90 °C using 3% beeswax as oleogelator agent. The best oleogel obtained was stored for 40 days and evaluated for its stability every 10 days followed by testing as a shortening replacer in a cake. The results showed that the higher oleogelation temperature, the greater the hardness and oil binding of oleogel, leading to lower acid and peroxide values. The best oleogelation temperature was obtained at 90 °C with hardness, oil binding capacity, acid, and peroxide values of 0.08±0.01N, 98.31±0.39%, 0.70±0.03 mg KOH/g, and 22.61±0.33 mek O2 /kg, respectively. During 40 days of storage, the hardness and oil binding capacity decreased while the acid, peroxide, and TBA values increased. Additionally, the crystal structure of peanut oil oleogel was affected during storage. The application in cake resulted in lower viscosity of cake batter and a higher yellow index compared to the control (shortening), but the texture profile of cake formulated with oleogel and shorthening (contorl)  was not significantly different. This implied that shortening replacement with peanut oil oleogel in baked products was feasible.
Ethanolysis Pretreatment of Crude Palm Oil in High Shear Reactor Hidrotunnisa, Hidrotunnisa; Yanti, Rini; Hidayat, Chusnul
agriTECH Vol 45, No 1 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.81499

Abstract

Crude palm oil (CPO) is characterized by high carotenoid content, dissolving in the oil system and causing a difficult extraction process. To overcome this problem, transesterification of CPO with ethanol was performed to improve the carotenoid extractability using sodium hydroxide as a catalyst. Therefore, this study aimed to investigate the effect of ethanolysis parameters on the carotenoid and fatty acid ethyl ester (FAEE) content of CPO. FAEE content was quantified by thin-layer chromatography and total carotenoid content (TCC) was determined through a UV-Vis spectrophotometer. Reaction time (15-150 min), catalyst concentration (0.65-3.25%), reaction temperature (3070 ℃), and stirring speed (500-2000 rpm) were determined by one factor at a time (OFAT) design. The results showed that ethanolysis time and temperature did not affect TCC along the study range, while ethanolysis time of 45 minutes and temperature of 70 ℃ increased FAEE content. Additionally, catalyst concentration of 1.95% and stirring speed at 2000 rpm increased TCC and FAEE. The best ethanolysis parameters were obtained at 45 minutes, the temperature of 50 ℃, catalyst concentration of 1.3%, and stirring speed of 2000 rpm, yielding FAEE 1358±16 ppm and TCC 99.9±0.2%. This showed that ethanolysis produced high FAEE content and improved carotenoids in the oil system.