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Uji Daya Hambat Ekstrak Daun Jamblang (Syzygium Cumini (L.) Skeels) Terhadap Pertumbuhan Bakteri Streptococcus Pyogenes dan Proteus Mirabilis Venny Patricia; Wawan Sofwan Zaini; Ahmad Yani; Siti Atikah
Jaringan Laboratorium Medis Vol 3, No 1 (2021): May 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i1.7932

Infectious diseases are a problem for public health and can increase patient morbidity and mortality. Bacteria that cause infectious diseases include Streptococcus pyogenes and Proteus mirabilis. Inappropriate use of antibiotics can cause resistance problems and the effects of undesirable drugs. Jamblang (Syzygium cumini) leaf extract can be used as a natural antibacterial drug. There is a study showing the content of active compounds in the leaves of Jamblang are Flavonoids, tannins, alkaloids, and saponins are components of chemical compounds that are suspected as antibacterial. This study aims to determine the antibacterial activity of Jamblang leaf extract against Streptococcus pyogenes and Proteus mirabilis bacteria. The method used is the Minimum Inhibitory Concentration method using well diffusion. The study used Jamblang leaf extract with various concentrations of 10%, 20%, 30%, and 40%, the test bacteria used were Streptococcus pyogenes and Proteus mirabilis, using positive control of chloramphenicol, and negative control of 10% DMSO. The results showed that Jamblang leaf extract can inhibit the growth of Streptococcus pyogenes and Proteus mirabilis bacteria in all concentrations. In the Kruskall Wallis Non-Parametric statistical test the two bacteria obtained P 0.05.In Streptococcus pyogenes bacteria the value of P = 0.031, and Proteus mirabilis bacteria the value of P = 0.015. These results indicate a real difference in this study.

Identifikasi Bakteri pada Luka Penderita Diabetes Melitus di Rumah Perawatan Luka Diabetes Venny Patricia; Ahmad Yani; Salwa Salsabila; Rr. Sri Isjworowati
Jaringan Laboratorium Medis Vol 5, No 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.8780

Diabetes mellitus (DM) is a metabolic disease that occurs due to hyperglycemia. In Indonesia it self, DM has a relatively high prevalence, this is evidenced because Indonesia is in the category of the top 10 countries in the world. The type of research used is descriptive research, namely identifying bacteria and testing sensitivity on wounds of diabetics with diabetes mellitus by performing cultures that are examined macroscopically and microscopically with the number of respondents obtained by as many as 17 people in 4 weeks. The results of this study obtained the prevalence of the types of bacteria that are (82.35%) gram-negative and (17.65%) gram-positive. The gram negatives included Proteus mirabilis (17.65%), Proteus morgani (5.88%), Citrobacter diversus (23.54%), Pseudomonas aeruginosa (11.76%), Escherichia coli (5.88%), Enterobacter agglomerans (5.88%) and Enterobacter cloacae (11.76%). While the gram-positive include Staphylococcus aureus (17.65%) with antibiotic sensitivity results whose sensitivity is in the yellow zone, which is around 50-75%, namely ciprofloxacin and meropenem antibiotics are in the green zone, which is around 76-100%.

Potensi Media Transport Modifikasi Sebagai Penunjang Pemeriksaan Kultur Bakteri Patricia, Venny; Yani, Ahmad; Nur fathonah, Amanda Kukuh
Jaringan Laboratorium Medis Vol 6, No 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10574

Bacterial culture sample transport is a critical point in the culture chain from sampling to final diagnosis. The ideal transport medium should be able to nurture the ability of bacteria to thrive coming from the sample without allowing them to grow. Transport media that already exist in Indonesia, namely in the form of powder, tend to be very expensive in price and transport media that can be used directly besides being expensive have a fast expiration period, which will increase the cost of treatment and services in hospitals, an innovation is needed by making alternative transport media from materials that are easily available around us that can be useful for taking samples. The purpose of this study is to see the potential of alternative transport media so that it can support diagnostics in bacterial culture. The study method uses an experimental laboratory with a true-experimental post-test-only design. In testing alternative transport media, sampling from diabetic foot ulcer patients using tofu pulp transport media (MTAT) and ICU swab beds with coconut charcoal transport media (MTAK). Samples of each media were taken, and as many as 20 samples were taken. In addition to treatment samples, each sample is confirmed using standard transport media as its control. This study obtained dominant bacteria from diabetic ulcer samples on alternative MTAT Citrobacter sp 6 (30%) and Control Citrobacter sp 8 (40%). Meanwhile, the ICU swab bed samples obtained dominant bacteria MTAK Bacillus sp 10 (50%) and Control 11 (55%). The conclusion of these two alternative transport media can be used as alternative transport media because they have the same growth results compared to standard media, and it can be ascertained that it is really bacteria that usually grow without contamination.

Uji Daya Hambat Ekstrak Daun Jamblang (Syzygium Cumini (L.) Skeels) Terhadap Pertumbuhan Bakteri Streptococcus Pyogenes dan Proteus Mirabilis Patricia, Venny; Zaini, Wawan Sofwan; Yani, Ahmad; Atikah, Siti
Jaringan Laboratorium Medis Vol. 3 No. 1 (2021): May 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i1.7932

Infectious diseases are a problem for public health and can increase patient morbidity and mortality. Bacteria that cause infectious diseases include Streptococcus pyogenes and Proteus mirabilis. Inappropriate use of antibiotics can cause resistance problems and the effects of undesirable drugs. Jamblang (Syzygium cumini) leaf extract can be used as a natural antibacterial drug. There is a study showing the content of active compounds in the leaves of Jamblang are Flavonoids, tannins, alkaloids, and saponins are components of chemical compounds that are suspected as antibacterial. This study aims to determine the antibacterial activity of Jamblang leaf extract against Streptococcus pyogenes and Proteus mirabilis bacteria. The method used is the Minimum Inhibitory Concentration method using well diffusion. The study used Jamblang leaf extract with various concentrations of 10%, 20%, 30%, and 40%, the test bacteria used were Streptococcus pyogenes and Proteus mirabilis, using positive control of chloramphenicol, and negative control of 10% DMSO. The results showed that Jamblang leaf extract can inhibit the growth of Streptococcus pyogenes and Proteus mirabilis bacteria in all concentrations. In the Kruskall Wallis Non-Parametric statistical test the two bacteria obtained P <0.05.In Streptococcus pyogenes bacteria the value of P = 0.031, and Proteus mirabilis bacteria the value of P = 0.015. These results indicate a real difference in this study.

Identifikasi Bakteri pada Luka Penderita Diabetes Melitus di Rumah Perawatan Luka Diabetes Patricia, Venny; Yani, Ahmad; Salsabila, Salwa; Isjworowati, Rr. Sri
Jaringan Laboratorium Medis Vol. 5 No. 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.8780

Diabetes mellitus (DM) is a metabolic disease that occurs due to hyperglycemia. In Indonesia it self, DM has a relatively high prevalence, this is evidenced because Indonesia is in the category of the top 10 countries in the world. The type of research used is descriptive research, namely identifying bacteria and testing sensitivity on wounds of diabetics with diabetes mellitus by performing cultures that are examined macroscopically and microscopically with the number of respondents obtained by as many as 17 people in 4 weeks. The results of this study obtained the prevalence of the types of bacteria that are (82.35%) gram-negative and (17.65%) gram-positive. The gram negatives included Proteus mirabilis (17.65%), Proteus morgani (5.88%), Citrobacter diversus (23.54%),Â Pseudomonas aeruginosa (11.76%), Escherichia coli (5.88%), Enterobacter agglomerans (5.88%) and Enterobacter cloacae (11.76%). While the gram-positive include Staphylococcus aureus (17.65%) with antibiotic sensitivity results whose sensitivity is in the yellow zone, which is around 50-75%, namely ciprofloxacin and meropenem antibiotics are in the green zone, which is around 76-100%.

Potensi Media Transport Modifikasi Sebagai Penunjang Pemeriksaan Kultur Bakteri Patricia, Venny; Yani, Ahmad; Nur fathonah, Amanda Kukuh
Jaringan Laboratorium Medis Vol. 6 No. 1 (2024): May 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i1.10574

Bacterial culture sample transport is a critical point in the culture chain from sampling to final diagnosis. The ideal transport medium should be able to nurture the ability of bacteria to thrive coming from the sample without allowing them to grow. Transport media that already exist in Indonesia, namely in the form of powder, tend to be very expensive in price and transport media that can be used directly besides being expensive have a fast expiration period, which will increase the cost of treatment and services in hospitals, an innovation is needed by making alternative transport media from materials that are easily available around us that can be useful for taking samples. The purpose of this study is to see the potential of alternative transport media so that it can support diagnostics in bacterial culture. The study method uses an experimental laboratory with a true-experimental post-test-only design. In testing alternative transport media, sampling from diabetic foot ulcer patients using tofu pulp transport media (MTAT) and ICU swab beds with coconut charcoal transport media (MTAK). Samples of each media were taken, and as many as 20 samples were taken. In addition to treatment samples, each sample is confirmed using standard transport media as its control. This study obtained dominant bacteria from diabetic ulcer samples on alternative MTAT Citrobacter sp 6 (30%) and Control Citrobacter sp 8 (40%). Meanwhile, the ICU swab bed samples obtained dominant bacteria MTAK Bacillus sp 10 (50%) and Control 11 (55%). The conclusion of these two alternative transport media can be used as alternative transport media because they have the same growth results compared to standard media, and it can be ascertained that it is really bacteria that usually grow without contamination.

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