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The Effectiveness o f Entomopathogenic Fungi Beauveria bassiana with the Addition of Insect Growth Regulator Lufenuron f or Controlling Bactrocera carambolae Hadi, Mochammad Syamsul; Himawan, Toto; Aini, Luqman Qurata
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The study of the effectiveness of entomopathogenic fungus Beauveria bassiana (Bals) Vuill. with the addition of an insect growth regulator (IGR) lufenuron to control the fruit fly Bactrocera carambolae (Drew and Hancock) (Diptera: Tephritidae) was conducted at the Laboratory of Toxicology and Laboratory of Entomology, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya Malang from March 2012 until December 2012. This study examined the effect of lufe-nuron to the development and sporulation of B. bassiana and the effectiveness of the fungus B. bassiana in combination with several concentrations of lufenuron (0.5, 1, 1.5 mL/L) on the mortality of fruit fly larvaB. carambolae. The results showed that the addition of lufenuron at the concentration of 1 mL/L could significantly increase the sporulation of B. bassiana. The addition of B. bassiana at 108 spores/mL combined with lufenuron at the concentration of 1 mL/L applied to compost pupation medium of larva of B. carambolae is significantly able to suppress the formation of the fruit fly pupa and imago of B. carambolae
Bioactivity of Sweet Flag (Acorus calamus Linnaeus) Essential Oils against Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Melani, Dewi; Himawan, Toto; Afandhi, Aminudin
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.04

Abstract

The study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a com-pletely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and anti-feedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatog-raphy-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone. Keywords:  Acorus calamus, essential oils, mortality,  antifeedant,  Spodoptera lituraThe study aims to determine the chemical compounds, toxicity, and antifeedant activity of sweet flag (Acorus calamus) essential oils against third instar larvae of Spodoptera litura. The study was conducted using a completely randomized design (CRD) using various concentration of the essential oils (103, 2 × 103, 3 × 103, 4 × 103, 5 × 103 ppm). Mortality and antifeedant activity was observed 24 hours after treatment.  Toxicity and antifeedant activity values were 92.5% and 79.3%, respectively, with an LC50 value 586.96 ppm. Gas chromatography-mass spectrometry analysis showed that essential oil of A. calamus consists of five chemical compounds: methyl isoeugenol, 3.9-decadien-ol-1,3-methyl-6-(1-methylethenyl), 4-pentyl-1-(4propylcyclohexyl)1cyclohexene, γ-asarone and β asarone.
Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Subarjah, Cecep; Himawan, Toto; Puspitarini, Retno Dyah
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Bioactivity of Citrus hystrix D.C. Leaf Extract Against Cigarette Beetle Lasioderma serricorne (F.) Ikawati, Silvi; Dhuha, Moh. Syamsu; Himawan, Toto
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.01

Abstract

The major control of pest stored Lasioderma serricorne for using synthetic pesticides, look like phosphine. Although quite effective, synthetic pesticides have a negative effect on the environment, such as pest re-sistance, deadly natural enemies, residues that are harmful to the environment and human health. The use of botanical pesticides as an alternative control be the focus this study. Botanical pesticides are selective to the target, safe for non-target insects and the environment. This research studies the repellent and fumigant activi-ty of the leaf extract of Citrus hystrix against all L. serricorne life stage. The results of GC-MS analysis of leaf crude extracts of C. hystrix with N-hexane solvent showed the highest percentage in the citronellal compound (86.43%). C. hystrix leaf extract showed stronger fumigant against pupae and eggs, compared adults, and larvae. The toxicity of the leaf extract of C. hystrix increased with increasing concentration. At a concentration of 60 ppm, fumigant activity showed the toxicity of 98.75% (pupae), 93.75% (eggs), 86.25% (adults) and 76.25% (larvae). Sequentially the LC50 value of fumigant activity from the highest to the lowest as follows; larvae 47.56 ppm, adults 43.42 ppm, eggs 31.61 and pupae 29.63 ppm. Extract of leaves of C. hystrix, have character repellent against L. serricorne. At a concentration of 60 ppm the IR value of 66% including repellent class IV, which means strong repellent level. Based on the results of the research, extracts of leaves of C. hystrix has a fumigant activity and repellent for controlling L. serricorne.
Co-Authors Achmad Nasroh Kuswadi Adne Yudansha Adne Yudansha Agus Suryanto Aji Santoso Akhmad Rizali Alorisa Tirta Ardiyati Aminuddin Affandi Aminudin Afandhi Andy Andy Arif Budi Prasetya Asep Ruhiyat Suherlan Avi Damayanthi Avi Damayanthi Bambang Tri Rahardjo Bambang Tri Raharjo BANDUNG SAHARI Choirul Mahdianto Damaiyanti, D.R.R. Dani Muara Histo Dewi Fajarwati Dewi Fajarwati Dhuha, Moh. Syamsu Edi Priyo Utomo Eko Wahyu Budi Darmawan Eko Wahyu Budi Darmawan Emha Dwi Rifqi Rafid Fadhila Herdatiarni Fery Abdul Choliq Fitriani, Ima Gatot Mudjiono Gatot Mudjiono Hagus Tarno Hari Sutrisno Hari Sutrisno Hasan Suprapto Hudiwaku, Syarron Irmawati Irmawati Isnainy Dinul Mursyalati Yus Isnainy Dinul Mursyalati Yus Kolopaking, Budiarto Kristiana Sri Wijayanti Lestari, Martina S Lindia Rahayu Widia Santi Ludji Pantja Astuti Ludji Pantja Astuti Ludji Pantja Astuti Ludji Pantja Astuti Ludji Pantja Astuti Luqman Qurata Aini Maria Ulfa Martina S Lestari Ma’arif, Ridwan Ahmad Melani, Dewi Mochammad Syamsul Hadi Muhamad Ari Bachtiar Muhamad Luthfie Tri Meiadi Nadiah, Annisrien Nely Yuliastanti Nindya Resha Pramesti Nindya Resha Pramesti Novi Dian Nathasia Novita Yuniasari Nur Hayati Octavia, Evy P Wijayanto P Wijayanto Primastya Dinarwika Primastya Dinarwika Putra, Heri Julistiyan Bima Retno Dyah Puspitarini Richa Ratih Damayanti Richa Ratih Damayanti Rina Rachmawati Rina Rachmawati Rizcki Nugraha Laksana Roza Anugraha Wiranata Roza Anugraha Wiranata Rurini Retnowati S. Karindah S. Karindah Saputra, Tri Ichsan Septi Mauludiana Silvi Ikawati Silvi Ikawati, Silvi Sitawati Sitawati Sri Heriza Sri Heriza Sri Karindah Sri Karindah Sri Karindah Subarjah, Cecep Sulistiyono Sulistiyono Sutrisno Sutrisno Sutrisno, Sutrisno Syarron Hudiwaku V. S Reychel Yogo Setiawan Yuliastanti, Nely Yuniasari, Novita