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Journal : Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)

Effect of breeds on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center Novianti, Irida; Purwantara, Bambang; Herwijanti, Enniek; Nugraha, Chairdin Dwi; Putri, Rafika Febriani; Furqon, Ahmad; Septian, Wike Andre; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 2 (2020): August 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.02.10

Abstract

Madura and Bali cattle are two of Indonesian indigenous breeds. One possible method to increase their productivity is through artificial insemination (AI) using frozen semen. This study conducted at the Singosari National Artificial Insemination Center, East Java, Indonesia, to evaluate the semen characteristics of different bull breeds ranging in age from 7 to 10 years and to provide useful recommendations regarding the efficacy of older cattle. A total of 2016 ejaculation data recorded from Indonesian indigenous cattle (Madura and Bali bulls) and Bos taurus bulls (Simmental) were used in this study to analyze semen volume, sperm motility, and sperm concentration. Results revealed differences in semen volume, sperm motility, and sperm concentration among bull breeds (p < 0.05). Simmental bulls have the highest semen volume (p < 0.05) compared with Bali and Madura bulls. Base on sperm motility, Bali bulls showed the highest average value (p < 0.05) compared with Simmental and Madura bulls. Sperm concentration was the highest (p < 0.05) among Bali bulls compared with Simmental and Madura bulls. Another important finding was that the average semen volume, sperm motility, and sperm concentration of older bulls fulfilled the standard criteria established for frozen semen processing. Therefore, it would be possible to improve the performance of these breeds through artificial insemination using frozen semen of Madura and Bali bulls, which can be more widely distributed to farmers to increase their herd productivity. This study also indicates that Indonesian indigenous bulls have superior semen characteristics than Simmental bulls.
Novel Mutations in The Coding Region of The Third Exon of Myostatin (MSTN) Gene in Madura Cattle irida novianti; Rafika Febriani Putri; Doni Herviyanto; Ahmad Furqon; Wike Andre Septian; Kuswati Kuswati; suyadi suyadi
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.08

Abstract

Molecular information using gene variants could support livestock selection to improve livestock productivity. This study aimed to identify the variants in part of the MSTN gene (part of the third exon coding region), a gene known for its significant effect on muscle growth. DNA was isolated from 95 Madura cattle blood samples collected in Pamekasan, Madura. PCR was used to amplify to MSTN region in part of the third exon, and one-way sequencing was conducted to obtain the sequence from the amplified product. Six SNPs were identified. Four SNPs were novel mutations, and the other two SNPs were recorded in the Ensembl database (rs466598800 and rs211583837). Among the identified novel mutations, one mutation in c.1301G/T caused a stop codon for the TT genotype cattle. However, no cattle carried the TT genotype. Three other mutations in c.1324, c.1373, and c.1399 were missense mutations that change the amino acids Q311L, A318P, and L335B, respectively. Most of the cattle had heterozygote genotypes for all the identified SNPs. It could also be noted that there were sequence differences in the part of the third exon identified between Madura cattle and Zebu cattle (by Tantia et al., 2006).