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All Journal Vegetalika Natural Science: Journal of Science and Technology AL KAUNIYAH Jurnal Biologi Universitas Andalas Biocelebes Kultivasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Agrotek Tropika Jurnal Hilirisasi IPTEKS (JHI) Agro Bali: Agricultural Journal Bioscientist : Jurnal Ilmiah Biologi Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat
Suwirmen, Suwirmen
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Physics Public Health Social Sciences Veterinary Other

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Enhancing the Growth of Water Spinach (Ipomoea reptans L.) through the Application of Chicken Bone Waste-Based Fertilizer in Ultisol Soil Rahima Taher; Suwirmen; Zozy Aneloi Noli
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.20025

Abstract

This study aimed to evaluate the effects of the dosage and application frequency of chicken bone meal on the growth of water spinach (Ipomoea reptans L.). The experiment employed a factorial design within a completely randomized design (CRD) consisting of two factors, namely chicken bone meal dosage and application frequency. The observed parameters included plant height, number of leaves, and plant dry weight. The results showed that both the dosage and application frequency of chicken bone meal significantly affected the growth of water spinach. A dosage of 60 g produced the highest dry weight, reaching 20.76 g, compared with 6.10 g in the control treatment, while an application frequency of once per week resulted in better plant growth. The interaction effect indicated that the A3B1 combination produced the best growth performance, with dry weight reaching 24.97 g. Therefore, the application of chicken bone meal at an appropriate dosage and frequency can optimize the growth of water spinach.
Effect of Botanical Pesticides on Inhibiting Colletotrichum sp. Causing Anthracnose in Avocado (Persea americana Mill.) In Vitro Anisa Rahman Rusiati; Feskaharny Alamsjah; Riska; Suwirmen
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.14567

Abstract

Anthracnose caused by Colletotrichum sp. is a major postharvest disease of avocado (Persea americana Mill.) that leads to significant economic losses during storage. This study aimed to evaluate the antifungal efficacy of essential oil-based botanical pesticides derived from lemongrass (Cymbopogon nardus L.), cinnamon (Cinnamomum burmannii L.), and wild betel (Piper aduncum L.) against Colletotrichum sp. in vitro. The experiment was arranged in a Completely Randomized Design (CRD) with nine treatments and three replications, using essential oil concentrations up to 1000 ppm in both single and combined formulations. Antifungal activity was assessed based on colony diameter and fungal biomass, and data were analyzed using Analysis of Variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT). All treatments significantly inhibited fungal growth compared to the control. The smallest colony diameter was recorded in the lemongrass treatment (12.80 mm), with the highest inhibition rate of 85.77%. The lowest fungal biomass was observed in the cinnamon treatment (20 mg). These results demonstrate very strong antifungal activity of the tested essential oils.This study highlights the novelty of utilizing locally sourced essential oils as botanical pesticide formulations targeting Colletotrichum sp., suggesting their potential as environmentally friendly alternatives for sustainable postharvest disease management.
Design and Experimental Validation of qPCR Primer for Drought-Responsive CaHsfA2 Gene in Capsicum annuum for Sustainable Crop Production Suwirmen; Zozy Aneloi Noli; Anthonie Agustien; Djong Hon Tjong; Amanda Nurhafitri
Jurnal Penelitian Pendidikan IPA Vol 12 No 5 (2026): In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i5.14897

Abstract

Drought stress severely reduces plant productivity by disrupting photosynthesis and cellular homeostasis. Plants respond through regulatory networks involving stress-responsive genes, including heat shock proteins (HSPs), regulated by heat shock transcription factors (HSFs). Among these, HsfA2 plays an important role in abiotic stress tolerance, including drought. Although CaHsfA2 expression has been reported in Capsicum annuum, validated primers for quantitative PCR (qPCR) analysis remain limited. This study aimed to design and validate specific primers for CaHsfA2 expression analysis. Primer candidates were designed from sequence NM_001324561.1 using Primer-BLAST and evaluated based on length, GC content, melting temperature (Tm), and self-complementarity. Total RNA was extracted from chili roots subjected to drought stress (50% field capacity) and nanopriming with Padina minor, followed by cDNA synthesis, gradient PCR and agarose gel electrophoresis.. Secondary structures were analyzed using OligoAnalyzer. Of ten primer pairs, four met the criteria, and primer set 1 (1_CaHsfA2) showed optimal characteristics (%GC 55%, Tm 60°C, product length 190 bp) and minimal secondary structure potential (ΔG > −9 kcal/mol). Gradient PCR (55.3–59.2°C) produced a single band (~193 bp), consistent with the predicted size (190 bp), with an optimal annealing temperature of 57.3°C, confirming its specificity and suitability for qPCR-based gene expression analysis under drought conditions.
Co-Authors - Zulhilmi . Mansyurdin Agusti Apriliani Akhyar Salim Amanda Nurhafitri Anisa Rahman Rusiati Anita Sari Anita Tri Astuti Anthoni Agustien Anthonie Agustien Asih Maharani Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Chairul Cleopatra Deswati Deswati Detty Intan Sari, Detty Intan Dezi Meutya Dapersal Dina Hayati Putri Djong Hon Tjong Efrizal Efrizal Efrizal Efrizal Elfans Bawalsyah S.A. Elvina Sari Emelta, Citra Endah Mutia Sari Erni Febriyanti Erni Febriyanti Ferry Lismanto Syaiful Feskaharny Alamsjah Fira Julia Putri Gian Wulandari Hanifah - Aini Henny Herwina Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Ivo Octaviani Izmiarti Izmiarti, Izmiarti Janra, Muhammad Nazri Jelita Putri Adisti Julita Julita Karimi, Kasman Kiki Ayunda Putri Kurniadi Ilham M. Idris M. Idris Mairawita, Mairawita Marta, Fepi Dwi Mayola Arda Media Media Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Nasril Nasir Nasril Nasir Naura Muthiah Arli Nazhira - Fadhilah Netty W Surya Novia Rika Deli Nurmiati Nurmiati Nurmiati Nurmiati Putri Aliyyanti Putri, Shintiya Rahmadhani Rahima Taher Rahmayati, Riesca Salsabilah Rahmi Rini Dwi Putri Resti Rahayu Reza Oktavia Ridha Permata Sari Riesca Salsabilah Rahmayati Rio Febrianto Riska Rizqa Zidna Chairafahmi Rusnam Rusnam Shayen, Millania Putri Sherin Dien Salsabila Solfiyeni Solfiyeni Suci Rahmadani Putri Syamsuardi Syamsuardi Tesri Maideliza Tesri Maideliza Tibrani Tibrani, Tibrani Titiek Rukmini Tressa Pratywi Gupitasari Wenny Rahma Gusni Winny Rabbial Firsti Aswendri Yella Prastika Yuda Yossi Eka Saputri Yulianda, Aldi Zakaria, Indra Junadi Zozi Aneloi Noli Zozy Aneloi Noli Zuhri Syam Zuhri Syam