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Induksi PLB Anggrek Vanda sumatrana Schltr. Liar Pada Media MS dengan Penambahan BAP dan NAA serta Ploidisasi dengan Kolkisin Hanifah - Aini; Mansyurdin - Mansyurdin; Suwirmen - Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 4 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.4.208-215.2015

Abstract

The study about PLB induction of wild Vanda sumatrana Schltr. on MS media suplement with BAP and NAA and ploidisation by colchicine treatment was conducted from December 2014 until November 2015 at the Laboratory of Genetics and Cell Biology and Laboratory of Plant Physiology and Tissue Culture, Biology department, Faculty of Mathematic and Natural Science, Andalas University, Padang. The study aimed to 1) knowing the best concentration of 6-Benzyl amino purin (BAP) and α-Naphtalene acetic acid (NAA) for Protocorm Like Bodies (PLB)  induction from shoot tip of V. sumatrana, 2) knowing the PLB response of V. sumatrana to concentrations and soak period of colchicine and 3) find the effective concentrations and soak period of colchicine to induce tetraploid on PLB of V. sumatrana. Shoot tips from in-vitro cultured of V. sumatrana  were subcultured on Murashinge and Skoog (MS) medium supplement with 3 mg/l BAP + 0,5 mg/l NAA, 3 mg/l BAP and 1,5 mg/l BAP. PLB of diploid V. sumatrana from the best treatment were soaked in 0.05% and 0.1% colchicine for 24 and 48 hours respectively in MS liquid medium, as control were set PLB without colchicine treatment. The results showed that MS medium supplemented with 1.5 mg/l BAP was the best formula to induce PLB. The highest percentage of survival rate of PLB and percentage of survived PLB regenerated shoot was obtained from 0.05% colchicine with 24 hours soak period treatment. The effective treatment to induce tetraploid on PLB of V. sumatrana Schltr. was obtained from 0.05% colchicine solution for 24 hours soak period.
Pengaruh Pupuk N dan Cekaman Kekeringan terhadap Pertumbuhan dan Kandungan Artemisinin Tanaman Artemisia vulgaris L. Elvina Sari; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 6, No 2 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.2.71-78.2018

Abstract

The research about effect of Nitrogen fertilizer and stress water on the growth and artemisinin content of Artemisia vulgaris L. has been conducted from December 2015 to March 2016 in Screen House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University, Padang. The purpose of this research were to the effect of  Nitrogen fertilizer, stress water, and their interaction between on the growth and artemisinin content of A. vulgaris. This research used Completely Randomized Design (CRD) in factorial with  four treatments ad three replications. The result showed that the number of leaves, plant height, length of roots, fresh weight, dry weight and of Artemisia vulgaris L. were increased significantly on N fertilizer treatments. The treatment of stress water 60% of volume capacity gave significantly effect on numbers of leaves and the highest content of Artemisinin was 80% volume capacity. The interaction of  N fertilizer and stress water did not showed significantly effect on the growth and artemisinin content of Artemisia vulgaris L.
Aklimatisasi Planlet Kantong Semar (Nepenthes gracilis Korth.) pada berbagai Campuran Media Tanam Tanah Ultisol Rio Febrianto; Suwirmen Suwirmen; Syamsuardi Syamsuardi
Jurnal Biologi Universitas Andalas Vol 4, No 2 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.2.%p.2015

Abstract

The research about Acclimatization of Pitcher Plant (Nepenthes gracilis Korth.) Planlet on Variety of Mixed Planting Medium Ultisol Soil had been conducted from May to August 2014 in Greenhouse and Plant Physiology Laboratory Department of Biology, Faculty of Natural Sciences, Andalas University. This study aimed to determine the influence of the media composition on the growth of seedlings of Nepenthes gracilis Korth. This study used completely randomized design (CRD) method with 5 treatments and 5 replications. The treatments of planting medium were consisted of A. Ultisol soil (control), B. Ultisol soil : manure : sand (1:1:1), C. Ultisol soil : manure : husk (1:1:1), D. Ultisol soil : manure : sand (2:1:1) and E. Ultisol soil : manure : husk (2:1:1). The results showed that treatment A i.e. ultisol soil (control) was the best growing medium for Nepenthes seedlings growth during acclimatization.
Aplikasi Pupuk Organik Cair Menggunakan Bioaktivator Mikroorganisme Indigenous HPPB Untuk Pertumbuhan Desmodium heterophyllum pada Tanah Bekas Tambang Batu Kapur PT. Semen Padang Rizqa Zidna Chairafahmi; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 6, No 1 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.1.6-14.2018

Abstract

The research about application of liquid organic fertilizers with indigenous microorganism bioactivator of HPPB for growth of Desmodium heterophyllum on limestone mined land at PT. Semen Padang, had been conducted from May to August 2015 in Nursery and Reforestation, Laboratory of Plant Physiology Department of Biology, and the Laboratory of Soil Science Faculty of Agriculture, Andalas University, Padang. The research aimed to determine the effect and concentration of Liquid Organic Fertilizers (LOF) by using indigenous microorganism bioactivator originated from HPPB in limestone mined land ofPT. Semen Padang. This study used Completely Randomized Design (CRD) with five treatments and four replications. The treatments were control without LOF (A), 10% LOF (B), 20% LOF (C), 30% LOF (D) and 40% LOF (E). The results showed that 10% LOF was the best concentration to increase the number of leaves (36,44), percentage of land cover (6,71%) and fresh weight of plants (5,15g).
Pemberian Beberapa Jenis Dan Konsentrasi Auksin Untuk Menginduksi Perakaran Pada Stek Pucuk Bayur (Pterospermum javanicum Jungh.) Dalam Upaya Perbanyakan Tanaman Revegetasi Agusti Apriliani; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

The research on effect of types and concentration of auxin on root induction of apical shoots Bayur (Pterospermum javanicum jungh.) in attempt to propagate of revegetation plants was conducted from May to August 2015 in the Nursery and Greening of Andalas University, and Laboratory of Plant Physiology Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The aimed to know the response of apical shoots Bayur (P. javanicum) on  root induction by providing some type and concentration of Auxin. This research used method of Complete Randomized Design (CRD) with seven treatments and three replications. The treatments consist of control, IBA 100 ppm, IBA 200 ppm, NAA 100 ppm, NAA 200 ppm, IAA 100 ppm, IAA 200 ppm. The result of this study showed that there was no respons of any treatments in inducing root of  apical shoots of Bayur (P. javanicum).Keywords: Auxin, Pterospermum javanicum, revegetation, root induction
Induksi Kalus Tanaman Puspa (Schima wallichii (DC.) Korth) dengan Penambahan Beberapa Konsentrasi Benzyl Amino Purin (BAP) dan 2,4-Diklorofenoksiasetat (2,4-D) Husri Meli; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 7, No 1 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.1.1-5.2019

Abstract

The research about Callus Induction of Puspa (Schima wallichii (DC.) Korth) with several concentrations addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetid acid  (2,4-D) had been done from October until November 2016 at Plant Physiology dan Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Science, Andalas University. The aim of this researh is to get the combination of 2,4-D and BAP to induce the best callus of Schima wallichii. This research used a Completely Randomized Design Method with 10 treatments and 3 replications. The result showed that combination of 2 ppm 2,4-D + 0,75 ppm BAP was the best concentration to induce callus of Schima wallichii.
The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in Various Planting Medium Suci Rahmadani Putri; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 7, No 2 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.2.91-99.2019

Abstract

The research about The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in various planting medium was held from May until August 2018 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was to find out the effects of various planting medium to the growth of shoot cutting Ant-nest Plant. This research used Completely Randomized Design (CRD) with five treatments (sand, husk charcoal, media moss, coconut fiber and fern’s root) and six replications. The results showed that shoot cutting that planted in sand, husk charcoal and fern had a highest life percentage (100%). Shoot cutting that planted in coconut fiber showed a highest height accretion. Shoot cutting that planted in media moss showed the highest root amount, longest root length and containing clorophyl level.
Pertumbuhan Bibit Karet (Hevea brasiliensis Mull Arg.) Setelah Pemberian Beberapa Dosis Fungi Mikoriza Arbuskula (FMA) Indigineous Dari Hutan Pendidikan Dan Penelitian Biologi (HPPB) Universitas Andalas Padang Akhyar Salim; Zozi Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

An experimental study on the growth of rubber seedling (Hevea brasiliensis Mull Arg.) after inoculated with various doses of indigineous Arbuscular Mycorrizhal Fungy (AMF) from Biology Forest Research Center (FRCB) Andalas University Padang has been done from February to September 2013 in wire house and Plant Physiology Laboratory, Biology Department, Andalas University, Padang. The study aimed to find appropriate doses of indigineous Arbuscular Mycorrizhal Fungy (AMF) to growth rubber seedling. The experimental used Completely Randomized Design (CRD) with four treatments (control, 10, 20, and 30 g inoculants) and six replications. The results showed that 10 g inoculant gave significant effect to growth and dry weight of rubber stem due to high percentage of root infection. However rubber assosiatian with mycorrizha are less dependency. Keywords : rubber, AMF, inoculant, seedling growth
Induksi kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4-Dichlorophenoxyacetic Acid(2,4-D) Nazhira - Fadhilah; Zozy Aneloi Noli; Suwirmen - Suwirmen
Jurnal Biologi Universitas Andalas Vol 4, No 4 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.4.216-222.2015

Abstract

The research about callus induction Artemisia vulgaris L. by giving several concentration 2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015 in Plant Physiology Laboratory and Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Andalas. The aim of this studywas found the effective concentration of 2,4-D to induce callus of A. vulgaris. The research used Completely Randomized Design (CRD) with 7 treatments and 4 replications. The treatments were : without 2,4-D (control); 0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L 2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe resultingcallusisyellowish green, brownish-green, yellow-brown, whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto increase fresh weight of callus.
Pengaruh Ekstrak Daun Tumbuhan Mikania micrantha Kunth. (Invasif) dan Cosmos sulphureus Cav. (Non Invasif) Terhadap Perkecambahan Jagung (Zea mays L.) Ayu Utami Rezki; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 6, No 2 (2018)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.6.2.79-83.2018

Abstract

The Research about the effects of extract from the invasive plant leaves of Mikania micrantha Kunth. and non-invasive plant leaves of Cosmos sulphureus Cav. on the germination of corn (Zea mays L.) has been conducted in July 2016 in Plant Physiology and Tissue Culture Laboratory, Biology Department, Mathematics and Natural Science Faculty, Andalas University, Padang. The aim of this research was to compare the effect of extract from the leaves of plants M. micrantha and C. sulphureus with several concentrations on the germination of corn. The research used experimental method with Completely Randomized Design (CRD) on Nested, 9 treatments and 4 replications. The treatments were factor A (type of plants, a1= Mikania micrantha and a2= Cosmos sulphureus) and factor B (leaf extract concentration, b0= 0%, b1= 20%, b= 40%, b3= 60%, b4= 80%). The results showed that the extract of the leaves from M. micrantha affected to reduced the fresh weight plants at concentration of 20%, where as in leaf extract of C. sulphureus affected to reduced the fresh weight plants at concentration of 40%.
Co-Authors - Zulhilmi . Mansyurdin Agusti Apriliani Akhyar Salim Anita Sari Anita Tri Astuti Anthoni Agustien Asih Maharani Aulya, Nailul Rahmi Ayola Pajrita Ayu Utami Rezki Azharia Khalida Chairul Chairul Chairul Chairul Chairul Cleopatra Deswati Deswati Detty Intan Sari, Detty Intan Dezi Meutya Dapersal Dina Hayati Putri Efrizal Efrizal Efrizal Efrizal Elfans Bawalsyah S.A. Elvina Sari Emelta, Citra Endah Mutia Sari Erni Febriyanti Erni Febriyanti Ferry Lismanto Syaiful Feskaharny Alamsjah Fira Julia Putri Gian Wulandari Hanifah - Aini Henny Herwina Hesti Dwi Marcellinna Husri Meli Iga Permata Hany Ilham, Kurniadi Ivo Octaviani Izmiarti Izmiarti, Izmiarti Janra, Muhammad Nazri Jelita Putri Adisti Julita Julita Karimi, Kasman Kiki Ayunda Putri Kurniadi Ilham M. Idris M. Idris Mairawita, Mairawita Marta, Fepi Dwi Mayola Arda Media Media Mildawati Mildawati Millania Putri Shayen Millania Putri Shayen Nasril Nasir Nasril Nasir Naura Muthiah Arli Nazhira - Fadhilah Netty W Surya Novia Rika Deli Nurmiati Nurmiati Nurmiati Nurmiati Putri Aliyyanti Putri, Shintiya Rahmadhani Rahmayati, Riesca Salsabilah Rahmi Rini Dwi Putri Resti Rahayu Reza Oktavia Ridha Permata Sari Riesca Salsabilah Rahmayati Rio Febrianto Rizqa Zidna Chairafahmi Rusnam Rusnam Shayen, Millania Putri Sherin Dien Salsabila Solfiyeni Solfiyeni Suci Rahmadani Putri Syamsuardi Syamsuardi Tesri Maideliza Tesri Maideliza Tibrani Tibrani, Tibrani Titiek Rukmini Tressa Pratywi Gupitasari Wenny Rahma Gusni Yella Prastika Yuda Yossi Eka Saputri Yulianda, Aldi Zakaria, Indra Junadi Zozi Aneloi Noli Zozy Aneloi Noli Zuhri Syam Zuhri Syam